P18893 (IL10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-10
Short name=IL-10

Alternative name(s):
Cytokine synthesis inhibitory factor
Short name=CSIF

Gene names

Name:	Il10
Synonyms:	Il-10

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-10 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	branching involved in labyrinthine layer morphogenesis Inferred from mutant phenotype PubMed 12842419. Source: MGI cellular response to estradiol stimulus Inferred from electronic annotation. Source: Compara defense response to bacterium Inferred from direct assay PubMed 10899905. Source: MGI immune response Inferred from electronic annotation. Source: Compara inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of B cell proliferation Inferred from sequence orthology PubMed 9184696. Source: MGI negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of chronic inflammatory response to antigenic stimulus Inferred from mutant phenotype PubMed 12452830. Source: MGI negative regulation of cytokine secretion involved in immune response Inferred from electronic annotation. Source: Compara negative regulation of growth of symbiont in host Inferred from mutant phenotype PubMed 12452830. Source: MGI negative regulation of interferon-gamma production Inferred from mutant phenotype PubMed 12452830. Source: MGI negative regulation of interleukin-12 production Inferred from mutant phenotype PubMed 15240714. Source: MGI negative regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara negative regulation of membrane protein ectodomain proteolysis Inferred from electronic annotation. Source: Compara negative regulation of myeloid dendritic cell activation Inferred from mutant phenotype PubMed 15240714. Source: MGI negative regulation of nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of tumor necrosis factor biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of tumor necrosis factor production Inferred from mutant phenotype PubMed 12452830. Source: MGI positive regulation of B cell apoptotic process Inferred from sequence orthology PubMed 9184696. Source: MGI positive regulation of MHC class II biosynthetic process Inferred from direct assay PubMed 11027433. Source: MGI positive regulation of cytokine secretion Inferred from electronic annotation. Source: Compara positive regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15187136. Source: BHF-UCL receptor biosynthetic process Inferred from electronic annotation. Source: Compara regulation of sensory perception of pain Inferred from electronic annotation. Source: Compara response to activity Inferred from electronic annotation. Source: Compara response to carbon monoxide Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to inactivity Inferred from electronic annotation. Source: Compara response to insulin stimulus Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from direct assay PubMed 10545106PubMed 10899905PubMed 10940872PubMed 15220916PubMed 15240714PubMed 16275384PubMed 16949315PubMed 17137799PubMed 19139201. Source: MGI
Molecular_function	cytokine activity Inferred from direct assay PubMed 11027433PubMed 11342597. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 178	160	Interleukin-10		PRO_0000015367
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 126		Ref.6
	Disulfide bond	80 ↔ 132		Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P18893 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 9A283E6FFC99312B	FASTA	178	20,641
	10 20 30 40 50 60 MPGSALLCCL LLLTGMRISR GQYSREDNNC THFPVGQSHM LLELRTAFSQ VKTFFQTKDQ 70 80 90 100 110 120 LDNILLTDSL MQDFKGYLGC QALSEMIQFY LVEVMPQAEK HGPEIKEHLN SLGEKLKTLR 130 140 150 160 170 MRLRRCHRFL PCENKSKAVE QVKSDFNKLQ DQGVYKAMNE FDIFINCIEA YMMIKMKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI." Moore K.W., Vieira P., Fiorentino D.F., Trounstine M.L., Khan T.A., Mosmann T.R. Science 248:1230-1234(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the mouse IL-10 gene and chromosomal localization of the mouse and human genes." Kim J.M., Brannan C.I., Copeland N.G., Jenkins N.A., Khan T.A., Moore K.W. J. Immunol. 148:3618-3623(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10." Windsor W.T., Syto R., Tsarbopoulos A., Zhang R., Durkin J., Baldwin S., Paliwal S., Mui P.W., Pramanik B., Trotta P.P. Biochemistry 32:8807-8815(1993) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37897 mRNA. Translation: AAA39274.1. M84340 Genomic DNA. Translation: AAA39275.1. AL513351 Genomic DNA. Translation: CAM14013.1. CH466520 Genomic DNA. Translation: EDL39722.1. BC120612 mRNA. Translation: AAI20613.1. BC137844 mRNA. Translation: AAI37845.1.
IPI	IPI00115602. IPI00654087.
PIR	A34853.
RefSeq	NP_034678.1. NM_010548.2.
UniGene	Mm.874.
3D structure databases
ProteinModelPortal	P18893.
SMR	P18893. Positions 39-175.
ModBase	Search...
PTM databases
PhosphoSite	P18893.
Proteomic databases
PRIDE	P18893.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000016673; ENSMUSP00000016673; ENSMUSG00000016529.
GeneID	16153.
KEGG	mmu:16153.
Organism-specific databases
CTD	3586.
MGI	MGI:96537. Il10.
Phylogenomic databases
eggNOG	NOG43085.
GeneTree	ENSGT00510000048535.
HOGENOM	HOG000072692.
HOVERGEN	HBG004110.
InParanoid	P18893.
KO	K05443.
OMA	KGYLGCQ.
OrthoDB	EOG4F1X4G.
Gene expression databases
CleanEx	MM_IL10.
Genevestigator	P18893.
GermOnline	ENSMUSG00000016529. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR020443. Interleukin-10/19/20/24. IPR020423. Interleukin-10_CS. IPR000098. Interleukin_10. [Graphical view]
PANTHER	PTHR11585. PTHR11585. 1 hit.
Pfam	PF00726. IL10. 1 hit. [Graphical view]
PRINTS	PR01294. INTRLEUKIN10.
SMART	SM00188. IL10. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00520. INTERLEUKIN_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	288951.
SOURCE	Search...

Entry information

Entry name

IL10_MOUSE

Accession

Primary (citable) accession number: P18893
Secondary accession number(s): Q0VBJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program