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Protein

Interleukin-10

Gene

Il10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

GO - Molecular functioni

  1. cytokine activity Source: MGI
  2. interleukin-10 receptor binding Source: GO_Central

GO - Biological processi

  1. aging Source: Ensembl
  2. branching involved in labyrinthine layer morphogenesis Source: MGI
  3. cellular response to estradiol stimulus Source: Ensembl
  4. cellular response to lipopolysaccharide Source: MGI
  5. defense response to bacterium Source: MGI
  6. immune response Source: Ensembl
  7. inflammatory response Source: MGI
  8. negative regulation of apoptotic process Source: Ensembl
  9. negative regulation of B cell proliferation Source: MGI
  10. negative regulation of chronic inflammatory response to antigenic stimulus Source: MGI
  11. negative regulation of cytokine secretion involved in immune response Source: MGI
  12. negative regulation of growth of symbiont in host Source: MGI
  13. negative regulation of inflammatory response Source: MGI
  14. negative regulation of interferon-gamma production Source: MGI
  15. negative regulation of interleukin-12 production Source: MGI
  16. negative regulation of interleukin-6 production Source: MGI
  17. negative regulation of membrane protein ectodomain proteolysis Source: MGI
  18. negative regulation of myeloid dendritic cell activation Source: MGI
  19. negative regulation of nitric oxide biosynthetic process Source: Ensembl
  20. negative regulation of tumor necrosis factor biosynthetic process Source: Ensembl
  21. negative regulation of tumor necrosis factor production Source: MGI
  22. positive regulation of B cell apoptotic process Source: MGI
  23. positive regulation of cytokine secretion Source: MGI
  24. positive regulation of JAK-STAT cascade Source: GO_Central
  25. positive regulation of MHC class II biosynthetic process Source: MGI
  26. positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  27. positive regulation of transcription, DNA-templated Source: MGI
  28. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  29. receptor biosynthetic process Source: MGI
  30. regulation of gene expression Source: MGI
  31. regulation of sensory perception of pain Source: Ensembl
  32. response to activity Source: Ensembl
  33. response to carbon monoxide Source: Ensembl
  34. response to drug Source: Ensembl
  35. response to glucocorticoid Source: MGI
  36. response to inactivity Source: Ensembl
  37. response to insulin Source: Ensembl
  38. response to molecule of bacterial origin Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-10
Short name:
IL-10
Alternative name(s):
Cytokine synthesis inhibitory factor
Short name:
CSIF
Gene namesi
Name:Il10
Synonyms:Il-10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:96537. Il10.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 178160Interleukin-10PRO_0000015367Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi30 ↔ 1261 Publication
Disulfide bondi80 ↔ 1321 Publication
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP18893.

PTM databases

PhosphoSiteiP18893.

Expressioni

Gene expression databases

CleanExiMM_IL10.
GenevestigatoriP18893.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi200603. 1 interaction.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4X51X-ray2.05A/B27-178[»]
ProteinModelPortaliP18893.
SMRiP18893. Positions 39-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG43085.
GeneTreeiENSGT00510000048535.
HOGENOMiHOG000072692.
HOVERGENiHBG004110.
InParanoidiP18893.
KOiK05443.
OMAiPHMLREL.
OrthoDBiEOG7C2R33.
TreeFamiTF333253.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000098. IL-10.
IPR020443. IL-10/19/20/24/26_fam.
IPR020423. IL-10_CS.
[Graphical view]
PANTHERiPTHR11585. PTHR11585. 1 hit.
PfamiPF00726. IL10. 1 hit.
[Graphical view]
PRINTSiPR01294. INTRLEUKIN10.
SMARTiSM00188. IL10. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00520. INTERLEUKIN_10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGSALLCCL LLLTGMRISR GQYSREDNNC THFPVGQSHM LLELRTAFSQ
60 70 80 90 100
VKTFFQTKDQ LDNILLTDSL MQDFKGYLGC QALSEMIQFY LVEVMPQAEK
110 120 130 140 150
HGPEIKEHLN SLGEKLKTLR MRLRRCHRFL PCENKSKAVE QVKSDFNKLQ
160 170
DQGVYKAMNE FDIFINCIEA YMMIKMKS
Length:178
Mass (Da):20,641
Last modified:October 3, 2012 - v2
Checksum:i9A283E6FFC99312B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37897 mRNA. Translation: AAA39274.1.
M84340 Genomic DNA. Translation: AAA39275.1.
AL513351 Genomic DNA. Translation: CAM14013.1.
CH466520 Genomic DNA. Translation: EDL39722.1.
BC120612 mRNA. Translation: AAI20613.1.
BC137844 mRNA. Translation: AAI37845.1.
CCDSiCCDS15265.1.
PIRiA34853.
RefSeqiNP_034678.1. NM_010548.2.
UniGeneiMm.874.

Genome annotation databases

EnsembliENSMUST00000016673; ENSMUSP00000016673; ENSMUSG00000016529.
GeneIDi16153.
KEGGimmu:16153.
UCSCiuc007cmu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37897 mRNA. Translation: AAA39274.1.
M84340 Genomic DNA. Translation: AAA39275.1.
AL513351 Genomic DNA. Translation: CAM14013.1.
CH466520 Genomic DNA. Translation: EDL39722.1.
BC120612 mRNA. Translation: AAI20613.1.
BC137844 mRNA. Translation: AAI37845.1.
CCDSiCCDS15265.1.
PIRiA34853.
RefSeqiNP_034678.1. NM_010548.2.
UniGeneiMm.874.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4X51X-ray2.05A/B27-178[»]
ProteinModelPortaliP18893.
SMRiP18893. Positions 39-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200603. 1 interaction.

PTM databases

PhosphoSiteiP18893.

Proteomic databases

PRIDEiP18893.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016673; ENSMUSP00000016673; ENSMUSG00000016529.
GeneIDi16153.
KEGGimmu:16153.
UCSCiuc007cmu.2. mouse.

Organism-specific databases

CTDi3586.
MGIiMGI:96537. Il10.

Phylogenomic databases

eggNOGiNOG43085.
GeneTreeiENSGT00510000048535.
HOGENOMiHOG000072692.
HOVERGENiHBG004110.
InParanoidiP18893.
KOiK05443.
OMAiPHMLREL.
OrthoDBiEOG7C2R33.
TreeFamiTF333253.

Miscellaneous databases

NextBioi288951.
PROiP18893.
SOURCEiSearch...

Gene expression databases

CleanExiMM_IL10.
GenevestigatoriP18893.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000098. IL-10.
IPR020443. IL-10/19/20/24/26_fam.
IPR020423. IL-10_CS.
[Graphical view]
PANTHERiPTHR11585. PTHR11585. 1 hit.
PfamiPF00726. IL10. 1 hit.
[Graphical view]
PRINTSiPR01294. INTRLEUKIN10.
SMARTiSM00188. IL10. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00520. INTERLEUKIN_10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI."
    Moore K.W., Vieira P., Fiorentino D.F., Trounstine M.L., Khan T.A., Mosmann T.R.
    Science 248:1230-1234(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure of the mouse IL-10 gene and chromosomal localization of the mouse and human genes."
    Kim J.M., Brannan C.I., Copeland N.G., Jenkins N.A., Khan T.A., Moore K.W.
    J. Immunol. 148:3618-3623(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10."
    Windsor W.T., Syto R., Tsarbopoulos A., Zhang R., Durkin J., Baldwin S., Paliwal S., Mui P.W., Pramanik B., Trotta P.P.
    Biochemistry 32:8807-8815(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiIL10_MOUSE
AccessioniPrimary (citable) accession number: P18893
Secondary accession number(s): Q0VBJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 3, 2012
Last modified: March 4, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.