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P18892 (BT1A1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Butyrophilin subfamily 1 member A1

Short name=BT
Gene names
Name:BTN1A1
Synonyms:BTN
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane. Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion By similarity.

Subunit structure

Seems to associate with xanthine dehydrogenase/oxidase By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein. Note: Secreted in association with the milk-fat-globule membrane during lactation By similarity.

Tissue specificity

Expressed in mammary tissue.

Post-translational modification

N-glycosylated By similarity. Ref.6

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Contains 1 B30.2/SPRY domain.

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.1
Chain27 – 526500Butyrophilin subfamily 1 member A1
PRO_0000014526

Regions

Topological domain27 – 242216Extracellular Potential
Transmembrane243 – 26927Helical; Potential
Topological domain270 – 526257Cytoplasmic Potential
Domain27 – 140114Ig-like V-type 1
Domain148 – 23487Ig-like V-type 2
Domain285 – 479195B30.2/SPRY

Amino acid modifications

Glycosylation551N-linked (GlcNAc...) (complex) Ref.6
Glycosylation2151N-linked (GlcNAc...) (hybrid) Ref.6
Disulfide bond50 ↔ 124 By similarity
Disulfide bond164 ↔ 218 By similarity

Experimental info

Sequence conflict161F → Y in AAB92578. Ref.4
Sequence conflict351Q → P in CAB07533. Ref.3
Sequence conflict2301E → D Ref.1
Sequence conflict2301E → D Ref.4

Secondary structure

....................................... 526
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18892 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: A14126802BD19284

FASTA52659,277
        10         20         30         40         50         60 
MAVFPNSCLA GCLLIFILLQ LPKLDSAPFD VIGPQEPILA VVGEDAELPC RLSPNVSAKG 

        70         80         90        100        110        120 
MELRWFREKV SPAVFVSREG QEQEGEEMAE YRGRVSLVED HIAEGSVAVR IQEVKASDDG 

       130        140        150        160        170        180 
EYRCFFRQDE NYEEAIVHLK VAALGSDPHI SMKVQESGEI QLECTSVGWY PEPQVQWRTH 

       190        200        210        220        230        240 
RGEEFPSMSE SRNPDEEGLF TVRASVIIRD SSMKNVSCCI RNLLLGQEKE VEVSIPASFF 

       250        260        270        280        290        300 
PRLTPWMVAV AVILVVLGLL TIGSIFFTWR LYKERSRQRR NEFSSKEKLL EELKWKRATL 

       310        320        330        340        350        360 
HAVDVTLDPD TAHPHLFLYE DSKSVRLEDS RQKLPEKPER FDSWPCVMGR EAFTSGRHYW 

       370        380        390        400        410        420 
EVEVGDRTDW AIGVCRENVM KKGFDPMTPE NGFWAVELYG NGYWALTPLR TPLPLAGPPR 

       430        440        450        460        470        480 
RVGVFLDYES GDIFFYNMTD GSHIYTFSKA SFSGPLRPFF CLWSCGKKPL TICPVTDGLE 

       490        500        510        520 
GVMVVADAKD ISKEIPLSPM GEDSASGDIE TLHSKLIPLQ PSQGVP 

« Hide

References

[1]"Cloning and analysis of cDNA encoding bovine butyrophilin, an apical glycoprotein expressed in mammary tissue and secreted in association with the milk-fat globule membrane during lactation."
Jack L.J.W., Mather I.H.
J. Biol. Chem. 265:14481-14486(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-47.
[2]Davey H.W., Ogg S.L., Husaini Y., Snell R.G., Korobko I.V., Mather I.H., Wilkins R.J.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Holstein-Friesian.
[3]Seyfert H.-M., Luethen F.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Blood.
[4]"Identification of five allelic polymorphisms in the bovine butyrophilin gene."
Husaini Y., Wilkins R.J., Davey H.W.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-286.
[5]Bhattacharya T.K., Misra S.S., Sheikh F.D., Dayal S., Vohra V., Kumar P., Sharma A.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 397-526.
Tissue: Blood.
[6]"Site-specific glycosylation of bovine butyrophilin."
Sato T., Takio K., Kobata A., Greenwalt D.E., Furukawa K.
J. Biochem. 117:147-157(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-55 AND ASN-215.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35551 mRNA. Translation: AAB39766.1.
AF005497 Genomic DNA. Translation: AAB62889.1.
Z93323 Genomic DNA. Translation: CAB07533.1.
AF037402 Genomic DNA. Translation: AAB92578.2.
AY491469 Genomic DNA. Translation: AAR85488.1.
PIRA37821.
RefSeqNP_776933.1. NM_174508.2.
UniGeneBt.59359.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HH8X-ray2.30A27-238[»]
ProteinModelPortalP18892.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000042450.

Proteomic databases

PaxDbP18892.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282157.
KEGGbta:282157.

Organism-specific databases

CTD696.

Phylogenomic databases

eggNOGNOG45068.
HOGENOMHOG000230860.
HOVERGENHBG050747.
InParanoidP18892.
KOK06712.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013162. CD80_C2-set.
IPR008985. ConA-like_lec_gl_sf.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
IPR006574. PRY.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSPR01407. BUTYPHLNCDUF.
SMARTSM00406. IGv. 1 hit.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805991.

Entry information

Entry nameBT1A1_BOVIN
AccessionPrimary (citable) accession number: P18892
Secondary accession number(s): O18955 expand/collapse secondary AC list , O18959, O46535, Q6RUS3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 15, 1998
Last modified: February 19, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references