Reviewed, UniProtKB/Swiss-Prot P18888 (SNF6_YEAST)

Names and origin

Protein names

Recommended name:
    Transcription regulatory protein SNF6
Alternative name(s):
    SWI/SNF complex component SNF6

Gene names

Name:	SNF6
Ordered Locus Names:	YHL025W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in transcriptional activation. Component of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.
Subunit structure	Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.
Subcellular location	Nucleus.
Miscellaneous	Present with 2900 molecules/cell in log phase SD medium. Ref.3

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative initiation
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP-dependent chromatin remodeling Inferred from direct assay. Source: SGD nucleotide-excision repair Inferred from mutant phenotype. Source: SGD positive regulation of transcription, DNA-dependent Inferred from direct assay. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	SWI/SNF complex Inferred from direct assay. Source: SGD
Molecular function	general RNA polymerase II transcription factor activity Traceable author statement. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAD23	P32628	1	EBI-17550,EBI-14668
RAD4	P14736	1	EBI-17550,EBI-14766
SNF11	P38956	1	EBI-17550,EBI-17560
TAF14	P35189	1	EBI-17550,EBI-18920

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Select]

Isoform Long (identifier: P18888-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P18888-2)

The sequence of this isoform is not available.

Note: Partially functional.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Transcription regulatory protein SNF6		PRO_0000072006
Regions
	Motif	2 – 8	7	Nuclear localization signal Potential
	Compositional bias	264 – 276	13	Gln-rich
Amino acid modifications
	Modified residue	165	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1990. Version 1. Checksum: ED9EA9F313F9079F	FASTA	332	37,607
	10 20 30 40 50 60 MGVIKKKRSH HGKASRQQYY SGVQVGGVGS MGAINNNIPS LTSFAEENNY QYGYSGSSAG 70 80 90 100 110 120 MNGRSLTYAQ QQLNKQRQDF ERVRLRPEQL SNIIHDESDT ISFRSNLLKN FISSNDAFNM 130 140 150 160 170 180 LSLTTVPCDR IEKSRLFSEK TIRYLMQKQH EMKTQAAELQ EKPLTPLKYT KLIAAAEDGS 190 200 210 220 230 240 RSTKDMIDAV FEQDSHLRYQ PDGVVVHRDD PALVGKLRGD LREAPADYWT HAYRDVLAQY 250 260 270 280 290 300 HEAKERIRQK EVTAGEAQDE ASLQQQQQQD LQQQQQVVTT VASQSPHATA TEKEPVPAVV 310 320 330 DDPLENMFGD YSNEPFNTNF DDEFGDLDAV FF
	Isoform Short (Sequence not available).	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae." Estruch F., Carlson M. Mol. Cell. Biol. 10:2544-2553(1990) [PubMed: 2188093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-165, MASS SPECTROMETRY.
[5]	"Structural analysis of the yeast SWI/SNF chromatin remodeling complex." Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L., Peterson C.L. Nat. Struct. Biol. 10:141-145(2003) [PubMed: 12524530] [Abstract] Cited for: 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, ELECTRON MICROSCOPY OF THE SWI/SNF COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M37132 Genomic DNA. Translation: AAA35063.1. M32028 Genomic DNA. Translation: AAA35064.1. U11582 Genomic DNA. Translation: AAB65078.1.
PIR	A36313.
RefSeq	NP_011838.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1226N.
IntAct	P18888. 36 interactions.
STRING	P18888.
Proteomic databases
PeptideAtlas	P18888.
Genome annotation databases
Ensembl	YHL025W; YHL025W; YHL025W; Saccharomyces cerevisiae. [Genome view]
GeneID	856360.
KEGG	sce:YHL025W.
NMPDR	fig|4932.3.peg.2976.
Organism-specific databases
CYGD	YHL025w.
SGD	S000001017. SNF6.
Phylogenomic databases
OMA	HDESDTI
OrthoDB	EOG9NZW9F
Gene expression databases
ArrayExpress	P18888.
Genevestigator	P18888.
GermOnline	YHL025W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	981817.

Entry information

Entry name

SNF6_YEAST

Accession

Primary (citable) accession number: P18888

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	November 24, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)