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P18882

- RDRP_I79A4

UniProt

P18882 - RDRP_I79A4

Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Kiev/59/1979 H1N1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
    Alternative name(s):
    Polymerase basic protein 1
    Short name:
    PB1
    RNA-directed RNA polymerase subunit P1
    Gene namesi
    Name:PB1
    OrganismiInfluenza A virus (strain A/Kiev/59/1979 H1N1)
    Taxonomic identifieri384495 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757RNA-directed RNA polymerase catalytic subunitPRO_0000078754Add
    BLAST

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini286 – 483198RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni249 – 2568Promoter-binding siteBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi187 – 1959Nuclear localization signalBy similarity
    Motifi203 – 21614Nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view]
    PfamiPF00602. Flu_PB1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18882-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS    50
    EKGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 100
    GIFENSCLET MEVVQQTRVD RLTQGRQTYD WTLNRNQPAA TALANTIEVF 150
    RSNGLTANES GRLIDFLKDV MESMDKEEIE ITTHFQRKRR VRDNMTKKMV 200
    TQRTIGKKKQ RVNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 250
    FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF 300
    TITGDNTKWN ENQNPRMFLA MITYITKNQP EWFRNILSIA PIMFSNKMAR 350
    LGKGYMFESK RMKLRTQIPA EMLASIDLKY FNESTRKKIE KIRPLLIDGT 400
    ASLSPGMMMG MFNMLSTVLG VSILNLGQKK YTKTTYWWDG LQSSDDFALI 450
    VNAPNHEGIQ AGVDRFYRTC KLVGINMSKK KSYINRTGTF EFTSFFYRYG 500
    FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 550
    FIKDYRYTYR CHRGDTQIQT RRSFELKKLW EQTRSKAGLL VSDGGPNLYN 600
    IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVVMPAHG 650
    PAKSMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF 700
    PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF SEIMKICSTI 750
    EELRRQK 757
    Length:757
    Mass (Da):86,465
    Last modified:November 1, 1990 - v1
    Checksum:i8C9DC40D78642EBB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38376 Genomic RNA. Translation: AAA43647.1.
    PIRiS06212.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38376 Genomic RNA. Translation: AAA43647.1 .
    PIRi S06212.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view ]
    Pfami PF00602. Flu_PB1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the full-size DNA copy of the influenza virus A/Kiev/59/79 (H1N1) PB1 gene protein."
      Petrov N.A., Golovin S.Y., Mamaev L.V., Netesov S.V., Vasilenko S.K.
      Bioorg. Khim. 13:1170-1175(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiRDRP_I79A4
    AccessioniPrimary (citable) accession number: P18882
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3