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Protein

Guanine nucleotide-binding protein G(o) subunit alpha

Gene

Gnao1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47MagnesiumBy similarity1
Metal bindingi182MagnesiumBy similarity1
Binding sitei326GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47GTPBy similarity8
Nucleotide bindingi176 – 182GTPBy similarity7
Nucleotide bindingi201 – 205GTPBy similarity5
Nucleotide bindingi270 – 273GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • aging Source: Ensembl
  • cellular process Source: MGI
  • dopamine receptor signaling pathway Source: MGI
  • forebrain development Source: Ensembl
  • G-protein coupled receptor signaling pathway Source: MGI
  • locomotory behavior Source: MGI
  • negative regulation of calcium ion transport Source: Ensembl
  • neuron projection development Source: Ensembl
  • positive regulation of GTPase activity Source: Ensembl
  • regulation of heart contraction Source: MGI
  • response to cytokine Source: Ensembl
  • response to drug Source: Ensembl
  • response to hydrogen peroxide Source: Ensembl
  • response to morphine Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-202040. G-protein activation.
R-MMU-4086398. Ca2+ pathway.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(o) subunit alpha
Gene namesi
Name:Gnao1
Synonyms:Gna0, Gnao
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:95775. Gnao1.

Subcellular locationi

  • Cell membrane 1 Publication
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  • cell body Source: MGI
  • dendrite Source: MGI
  • heterotrimeric G-protein complex Source: MGI
  • membrane Source: MGI
  • myelin sheath Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002037042 – 354Guanine nucleotide-binding protein G(o) subunit alphaAdd BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi3S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiP18872.
PeptideAtlasiP18872.
PRIDEiP18872.

PTM databases

iPTMnetiP18872.
PhosphoSitePlusiP18872.
SwissPalmiP18872.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031748.
CleanExiMM_GNAO1.
ExpressionAtlasiP18872. baseline and differential.
GenevisibleiP18872. MM.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS14.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199970. 8 interactors.
DIPiDIP-29921N.
IntActiP18872. 9 interactors.
MINTiMINT-89378.
STRINGi10090.ENSMUSP00000034198.

Structurei

Secondary structure

1354
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 41Combined sources6
Turni46 – 49Combined sources4
Helixi50 – 52Combined sources3
Helixi63 – 91Combined sources29
Helixi100 – 113Combined sources14
Turni114 – 116Combined sources3
Helixi122 – 131Combined sources10
Helixi135 – 141Combined sources7
Turni142 – 145Combined sources4
Helixi153 – 157Combined sources5
Helixi160 – 163Combined sources4
Turni172 – 176Combined sources5
Beta strandi186 – 188Combined sources3
Beta strandi199 – 201Combined sources3
Turni206 – 208Combined sources3
Helixi209 – 212Combined sources4
Helixi214 – 216Combined sources3
Beta strandi220 – 225Combined sources6
Helixi228 – 232Combined sources5
Beta strandi239 – 242Combined sources4
Helixi243 – 255Combined sources13
Helixi258 – 260Combined sources3
Beta strandi263 – 268Combined sources6
Helixi272 – 279Combined sources8
Helixi284 – 286Combined sources3
Helixi297 – 310Combined sources14
Helixi329 – 344Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90A/C22-354[»]
ProteinModelPortaliP18872.
SMRiP18872.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18872.

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP18872.
KOiK04534.
OMAiDSKMVCD.
OrthoDBiEOG091G0VUT.
PhylomeDBiP18872.
TreeFamiTF300673.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha-1 (identifier: P18872-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER
110 120 130 140 150
KTDSKMVCDV VSRMEDTEPF SAELLSAMMR LWGDSGIQEC FNRSREYQLN
160 170 180 190 200
DSAKYYLDSL DRIGAGDYQP TEQDILRTRV KTTGIVETHF TFKNLHFRLF
210 220 230 240 250
DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET TNRMHESLML
260 270 280 290 300
FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YPGSNTYEDA
310 320 330 340 350
AAYIQTQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG

CGLY
Length:354
Mass (Da):40,085
Last modified:January 23, 2007 - v3
Checksum:iB73A84F3BDB09F2C
GO
Isoform Alpha-2 (identifier: P18872-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-354: MLFDSICNNK...ANNLRGCGLY → KLFDSICNNK...AKNLRGCGLY

Show »
Length:354
Mass (Da):40,036
Checksum:iFCCD80172A673F46
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031251249 – 354MLFDS…GCGLY → KLFDSICNNKWFTDTSIILF LNKKDIFEEKIKKSPLTICF PEYTGPSAFTEAVAHIQGQY ESKNKSAHKEVYSHVTCATD TNNIQFVFDAVTDVIIAKNL RGCGLY in isoform Alpha-2. 1 PublicationAdd BLAST106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36777 mRNA. Translation: AAA37645.1.
M36778 mRNA. Translation: AAA74566.1.
BC051989 mRNA. Translation: AAH51989.1.
CCDSiCCDS22532.1. [P18872-1]
PIRiA36038. RGMSO1.
B36038. RGMSO2.
RefSeqiNP_001106855.1. NM_001113384.1. [P18872-2]
NP_034438.1. NM_010308.3. [P18872-1]
UniGeneiMm.251445.

Genome annotation databases

EnsembliENSMUST00000034198; ENSMUSP00000034198; ENSMUSG00000031748. [P18872-1]
ENSMUST00000125716; ENSMUSP00000114144; ENSMUSG00000031748. [P18872-1]
ENSMUST00000138659; ENSMUSP00000148550; ENSMUSG00000031748. [P18872-2]
GeneIDi14681.
KEGGimmu:14681.
UCSCiuc009mvk.2. mouse. [P18872-2]
uc009mvl.1. mouse. [P18872-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36777 mRNA. Translation: AAA37645.1.
M36778 mRNA. Translation: AAA74566.1.
BC051989 mRNA. Translation: AAH51989.1.
CCDSiCCDS22532.1. [P18872-1]
PIRiA36038. RGMSO1.
B36038. RGMSO2.
RefSeqiNP_001106855.1. NM_001113384.1. [P18872-2]
NP_034438.1. NM_010308.3. [P18872-1]
UniGeneiMm.251445.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90A/C22-354[»]
ProteinModelPortaliP18872.
SMRiP18872.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199970. 8 interactors.
DIPiDIP-29921N.
IntActiP18872. 9 interactors.
MINTiMINT-89378.
STRINGi10090.ENSMUSP00000034198.

PTM databases

iPTMnetiP18872.
PhosphoSitePlusiP18872.
SwissPalmiP18872.

Proteomic databases

PaxDbiP18872.
PeptideAtlasiP18872.
PRIDEiP18872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034198; ENSMUSP00000034198; ENSMUSG00000031748. [P18872-1]
ENSMUST00000125716; ENSMUSP00000114144; ENSMUSG00000031748. [P18872-1]
ENSMUST00000138659; ENSMUSP00000148550; ENSMUSG00000031748. [P18872-2]
GeneIDi14681.
KEGGimmu:14681.
UCSCiuc009mvk.2. mouse. [P18872-2]
uc009mvl.1. mouse. [P18872-1]

Organism-specific databases

CTDi2775.
MGIiMGI:95775. Gnao1.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP18872.
KOiK04534.
OMAiDSKMVCD.
OrthoDBiEOG091G0VUT.
PhylomeDBiP18872.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-202040. G-protein activation.
R-MMU-4086398. Ca2+ pathway.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

ChiTaRSiGnao1. mouse.
EvolutionaryTraceiP18872.
PROiP18872.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031748.
CleanExiMM_GNAO1.
ExpressionAtlasiP18872. baseline and differential.
GenevisibleiP18872. MM.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGNAO_MOUSE
AccessioniPrimary (citable) accession number: P18872
Secondary accession number(s): P18873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 173 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.