Guanine nucleotide-binding protein G(o) subunit alpha

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

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P18872 (GNAO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Guanine nucleotide-binding protein G(o) subunit alpha

Gene names

Name:	Gnao1
Synonyms:	Gna0, Gnao

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear. Ref.4
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS14. Ref.4
Sequence similarities	Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Keywords
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Myristate Palmitate
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cAMP nucleotide second messenger Inferred from Biological aspect of Ancestor. Source: RefGenome dopamine receptor signaling pathway Inferred from mutant phenotype. Source: MGI locomotory behavior Inferred from mutant phenotype. Source: MGI regulation of heart contraction Inferred from mutant phenotype. Source: MGI
Cellular component	heterotrimeric G-protein complex Traceable author statement. Source: MGI
Molecular function	G-protein beta/gamma-subunit complex binding Inferred from Biological aspect of Ancestor. Source: RefGenome G-protein coupled serotonin receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome GTP binding Inferred from mutant phenotype. Source: MGI GTPase activity Inferred from direct assay Ref.4. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Initiator methionine

Removed By similarity

Chain

2 – 354

353

Guanine nucleotide-binding protein G(o) subunit alpha

PRO_0000203704

Nucleotide binding

40 – 47

GTP By similarity

Nucleotide binding

176 – 182

GTP By similarity

Nucleotide binding

201 – 205

GTP By similarity

Nucleotide binding

270 – 273

GTP By similarity

Metal binding

Magnesium By similarity

Metal binding

182

Magnesium By similarity

Binding site

326

GTP; via amide nitrogen By similarity

Lipidation

N-myristoyl glycine By similarity

Lipidation

S-palmitoyl cysteine By similarity

Alternative sequence

249 – 354

106

MLFDS…GCGLY → KLFDSICNNKWFTDTSIILF LNKKDIFEEKIKKSPLTICF PEYTGPSAFTEAVAHIQGQY ESKNKSAHKEVYSHVTCATD TNNIQFVFDAVTDVIIAKNL RGCGLY in isoform Alpha-2.

VSP_031251

354

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform Alpha-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B73A84F3BDB09F2C Show »	FASTA	354	40,085
10 20 30 40 50 60 MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV KQMKIIHEDG 70 80 90 100 110 120 FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER KTDSKMVCDV VSRMEDTEPF 130 140 150 160 170 180 SAELLSAMMR LWGDSGIQEC FNRSREYQLN DSAKYYLDSL DRIGAGDYQP TEQDILRTRV 190 200 210 220 230 240 KTTGIVETHF TFKNLHFRLF DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET 250 260 270 280 290 300 TNRMHESLML FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YPGSNTYEDA 310 320 330 340 350 AAYIQTQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG CGLY « Hide
Isoform Alpha-2 [UniParc] [UniParc]. Checksum: FCCD80172A673F46 Show »	FASTA	354	40,036
10 20 30 40 50 60 MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV KQMKIIHEDG 70 80 90 100 110 120 FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER KTDSKMVCDV VSRMEDTEPF 130 140 150 160 170 180 SAELLSAMMR LWGDSGIQEC FNRSREYQLN DSAKYYLDSL DRIGAGDYQP TEQDILRTRV 190 200 210 220 230 240 KTTGIVETHF TFKNLHFRLF DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET 250 260 270 280 290 300 TNRMHESLKL FDSICNNKWF TDTSIILFLN KKDIFEEKIK KSPLTICFPE YTGPSAFTEA 310 320 330 340 350 VAHIQGQYES KNKSAHKEVY SHVTCATDTN NIQFVFDAVT DVIIAKNLRG CGLY « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go." Strathmann M., Wilkie T.M., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 87:6477-6481(1990) [PubMed: 1697681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-1 AND ALPHA-2). Tissue: Brain and Spermatid.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-1). Strain: C57BL/6. Tissue: Brain.
[3]	Lubec G., Klug S., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 22-32; 36-46; 55-100; 106-143; 146-177; 182-193; 199-206 AND 244-272, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain and Hippocampus.
[4]	"RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of galphao." Traver S., Bidot C., Spassky N., Baltauss T., De Tand M.F., Thomas J.L., Zalc B., Janoueix-Lerosey I., Gunzburg J.D. Biochem. J. 350:19-29(2000) [PubMed: 10926822] [Abstract] Cited for: FUNCTION, INTERACTION WITH RGS14.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

M36777 mRNA. Translation: AAA37645.1.
M36778 mRNA. Translation: AAA74566.1.
BC051989 mRNA. Translation: AAH51989.1.

IPI

IPI00115546.
IPI00230192.

PIR

RGMSO1. A36038.
RGMSO2. B36038.

RefSeq

NP_001106855.1. NM_001113384.1.
NP_034438.1. NM_010308.3.

UniGene

Mm.251445.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C7K	X-ray	2.90	A/C	22-354	[»]

ProteinModelPortal

P18872.

SMR

P18872. Positions 35-345.

ModBase

Search...

DIP

DIP-29921N.

IntAct

P18872. 4 interactions.

STRING

P18872.

PhosphoSite

P18872.

PRIDE

P18872.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENSMUST00000034198; ENSMUSP00000034198; ENSMUSG00000031748.
ENSMUST00000125716; ENSMUSP00000114144; ENSMUSG00000031748.

GeneID

14681.

KEGG

mmu:14681.

UCSC

uc009mvl.1. mouse.

CTD

2775.

MGI

MGI:95775. Gnao1.

HOVERGEN

HBG063184.

OMA

LERSRMI.

OrthoDB

EOG44F69B.

PhylomeDB

P18872.

ArrayExpress

P18872.

Bgee

P18872.

CleanEx

MM_GNAO1.

Genevestigator

P18872.

GermOnline

ENSMUSG00000031748. Mus musculus.

InterPro

IPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
[Graphical view]

Gene3D

G3DSA:1.10.400.10. GproteinA_insert. 1 hit.

K04534.

PANTHER

PTHR10218. Gprotein_alph_bd. 1 hit.

Pfam

PF00503. G-alpha. 1 hit.
[Graphical view]

PRINTS

PR00318. GPROTEINA.
PR00441. GPROTEINAI.

SMART

SM00275. G_alpha. 1 hit.
[Graphical view]

SUPFAM

SSF47895. Transducn_insert. 1 hit.

ProtoNet

Search...

NextBio

286592.

SOURCE

Search...

Entry name

GNAO_MOUSE

Accession

Primary (citable) accession number: P18872
Secondary accession number(s): P18873

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 128 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha-1 (identifier: P18872-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha-2 (identifier: P18872-2)

The sequence of this isoform differs from the canonical sequence as follows:
249-354: MLFDSICNNK...ANNLRGCGLY → KLFDSICNNK...AKNLRGCGLY