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P18872

- GNAO_MOUSE

UniProt

P18872 - GNAO_MOUSE

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Protein

Guanine nucleotide-binding protein G(o) subunit alpha

Gene

Gnao1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumBy similarity
Metal bindingi182 – 1821MagnesiumBy similarity
Binding sitei326 – 3261GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478GTPBy similarity
Nucleotide bindingi176 – 1827GTPBy similarity
Nucleotide bindingi201 – 2055GTPBy similarity
Nucleotide bindingi270 – 2734GTPBy similarity

GO - Molecular functioni

  1. corticotropin-releasing hormone receptor 1 binding Source: RefGenome
  2. G-protein beta/gamma-subunit complex binding Source: RefGenome
  3. G-protein coupled serotonin receptor binding Source: RefGenome
  4. GTPase activity Source: UniProtKB
  5. GTP binding Source: MGI
  6. metal ion binding Source: UniProtKB-KW
  7. mu-type opioid receptor binding Source: RefGenome
  8. signal transducer activity Source: RefGenome

GO - Biological processi

  1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
  2. aging Source: Ensembl
  3. cellular process Source: MGI
  4. dopamine receptor signaling pathway Source: MGI
  5. forebrain development Source: Ensembl
  6. G-protein coupled receptor signaling pathway Source: MGI
  7. locomotory behavior Source: MGI
  8. negative regulation of calcium ion transport Source: Ensembl
  9. neuron projection development Source: Ensembl
  10. positive regulation of GTPase activity Source: Ensembl
  11. regulation of heart contraction Source: MGI
  12. response to cytokine Source: Ensembl
  13. response to drug Source: Ensembl
  14. response to hydrogen peroxide Source: Ensembl
  15. response to morphine Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_205726. G-protein activation.
REACT_221970. Ca2+ pathway.
REACT_253342. PLC beta mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(o) subunit alpha
Gene namesi
Name:Gnao1
Synonyms:Gna0, Gnao
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:95775. Gnao1.

Subcellular locationi

Cell membrane 1 Publication

GO - Cellular componenti

  1. heterotrimeric G-protein complex Source: RefGenome
  2. intracellular Source: MGI
  3. membrane Source: MGI
  4. neuron projection Source: Ensembl
  5. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 354353Guanine nucleotide-binding protein G(o) subunit alphaPRO_0000203704Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

MaxQBiP18872.
PaxDbiP18872.
PRIDEiP18872.

PTM databases

PhosphoSiteiP18872.

Expressioni

Gene expression databases

BgeeiP18872.
CleanExiMM_GNAO1.
ExpressionAtlasiP18872. baseline and differential.
GenevestigatoriP18872.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS14.1 Publication

Protein-protein interaction databases

BioGridi199970. 7 interactions.
DIPiDIP-29921N.
IntActiP18872. 9 interactions.
MINTiMINT-89378.

Structurei

Secondary structure

1
354
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 416Combined sources
Turni46 – 494Combined sources
Helixi50 – 523Combined sources
Helixi63 – 9129Combined sources
Helixi100 – 11314Combined sources
Turni114 – 1163Combined sources
Helixi122 – 13110Combined sources
Helixi135 – 1417Combined sources
Turni142 – 1454Combined sources
Helixi153 – 1575Combined sources
Helixi160 – 1634Combined sources
Turni172 – 1765Combined sources
Beta strandi186 – 1883Combined sources
Beta strandi199 – 2013Combined sources
Turni206 – 2083Combined sources
Helixi209 – 2124Combined sources
Helixi214 – 2163Combined sources
Beta strandi220 – 2256Combined sources
Helixi228 – 2325Combined sources
Beta strandi239 – 2424Combined sources
Helixi243 – 25513Combined sources
Helixi258 – 2603Combined sources
Beta strandi263 – 2686Combined sources
Helixi272 – 2798Combined sources
Helixi284 – 2863Combined sources
Helixi297 – 31014Combined sources
Helixi329 – 34416Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90A/C22-354[»]
ProteinModelPortaliP18872.
SMRiP18872. Positions 35-345.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18872.

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiNOG322962.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP18872.
KOiK04534.
OMAiKEIYCHQ.
PhylomeDBiP18872.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Alpha-1 (identifier: P18872-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER
110 120 130 140 150
KTDSKMVCDV VSRMEDTEPF SAELLSAMMR LWGDSGIQEC FNRSREYQLN
160 170 180 190 200
DSAKYYLDSL DRIGAGDYQP TEQDILRTRV KTTGIVETHF TFKNLHFRLF
210 220 230 240 250
DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET TNRMHESLML
260 270 280 290 300
FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YPGSNTYEDA
310 320 330 340 350
AAYIQTQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG

CGLY
Length:354
Mass (Da):40,085
Last modified:January 23, 2007 - v3
Checksum:iB73A84F3BDB09F2C
GO
Isoform Alpha-2 (identifier: P18872-2) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-354: MLFDSICNNK...ANNLRGCGLY → KLFDSICNNK...AKNLRGCGLY

Show »
Length:354
Mass (Da):40,036
Checksum:iFCCD80172A673F46
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei249 – 354106MLFDS…GCGLY → KLFDSICNNKWFTDTSIILF LNKKDIFEEKIKKSPLTICF PEYTGPSAFTEAVAHIQGQY ESKNKSAHKEVYSHVTCATD TNNIQFVFDAVTDVIIAKNL RGCGLY in isoform Alpha-2. 1 PublicationVSP_031251Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36777 mRNA. Translation: AAA37645.1.
M36778 mRNA. Translation: AAA74566.1.
BC051989 mRNA. Translation: AAH51989.1.
CCDSiCCDS22532.1. [P18872-1]
PIRiA36038. RGMSO1.
B36038. RGMSO2.
RefSeqiNP_001106855.1. NM_001113384.1. [P18872-2]
NP_034438.1. NM_010308.3. [P18872-1]
UniGeneiMm.251445.

Genome annotation databases

EnsembliENSMUST00000034198; ENSMUSP00000034198; ENSMUSG00000031748. [P18872-1]
ENSMUST00000125716; ENSMUSP00000114144; ENSMUSG00000031748. [P18872-1]
GeneIDi14681.
KEGGimmu:14681.
UCSCiuc009mvk.2. mouse. [P18872-2]
uc009mvl.1. mouse. [P18872-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36777 mRNA. Translation: AAA37645.1 .
M36778 mRNA. Translation: AAA74566.1 .
BC051989 mRNA. Translation: AAH51989.1 .
CCDSi CCDS22532.1. [P18872-1 ]
PIRi A36038. RGMSO1.
B36038. RGMSO2.
RefSeqi NP_001106855.1. NM_001113384.1. [P18872-2 ]
NP_034438.1. NM_010308.3. [P18872-1 ]
UniGenei Mm.251445.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3C7K X-ray 2.90 A/C 22-354 [» ]
ProteinModelPortali P18872.
SMRi P18872. Positions 35-345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199970. 7 interactions.
DIPi DIP-29921N.
IntActi P18872. 9 interactions.
MINTi MINT-89378.

PTM databases

PhosphoSitei P18872.

Proteomic databases

MaxQBi P18872.
PaxDbi P18872.
PRIDEi P18872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034198 ; ENSMUSP00000034198 ; ENSMUSG00000031748 . [P18872-1 ]
ENSMUST00000125716 ; ENSMUSP00000114144 ; ENSMUSG00000031748 . [P18872-1 ]
GeneIDi 14681.
KEGGi mmu:14681.
UCSCi uc009mvk.2. mouse. [P18872-2 ]
uc009mvl.1. mouse. [P18872-1 ]

Organism-specific databases

CTDi 2775.
MGIi MGI:95775. Gnao1.

Phylogenomic databases

eggNOGi NOG322962.
HOGENOMi HOG000038730.
HOVERGENi HBG063184.
InParanoidi P18872.
KOi K04534.
OMAi KEIYCHQ.
PhylomeDBi P18872.
TreeFami TF300673.

Enzyme and pathway databases

Reactomei REACT_205726. G-protein activation.
REACT_221970. Ca2+ pathway.
REACT_253342. PLC beta mediated events.

Miscellaneous databases

ChiTaRSi Gnao1. mouse.
EvolutionaryTracei P18872.
NextBioi 286592.
PROi P18872.
SOURCEi Search...

Gene expression databases

Bgeei P18872.
CleanExi MM_GNAO1.
ExpressionAtlasi P18872. baseline and differential.
Genevestigatori P18872.

Family and domain databases

Gene3Di 1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10218. PTHR10218. 1 hit.
Pfami PF00503. G-alpha. 1 hit.
[Graphical view ]
PRINTSi PR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTi SM00275. G_alpha. 1 hit.
[Graphical view ]
SUPFAMi SSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go."
    Strathmann M., Wilkie T.M., Simon M.I.
    Proc. Natl. Acad. Sci. U.S.A. 87:6477-6481(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-1 AND ALPHA-2).
    Tissue: Brain and Spermatid.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-1).
    Strain: C57BL/6.
    Tissue: Brain.
  3. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 22-32; 36-46; 55-100; 106-143; 146-177; 182-193; 199-206 AND 244-272, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.
  4. "RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of galphao."
    Traver S., Bidot C., Spassky N., Baltauss T., De Tand M.F., Thomas J.L., Zalc B., Janoueix-Lerosey I., Gunzburg J.D.
    Biochem. J. 350:19-29(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RGS14.
  5. "Functional characterization of Galphao signaling through G protein-regulated inducer of neurite outgrowth 1."
    Nakata H., Kozasa T.
    Mol. Pharmacol. 67:695-702(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGNAO_MOUSE
AccessioniPrimary (citable) accession number: P18872
Secondary accession number(s): P18873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3