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P18872 (GNAO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein G(o) subunit alpha
Gene names
Name:Gnao1
Synonyms:Gna0, Gnao
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear. Ref.4

Subunit structure

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS14. Ref.4

Subcellular location

Cell membrane Ref.5.

Sequence similarities

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionTransducer
   PTMLipoprotein
Myristate
Palmitate
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 9501252. Source: MGI

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

aging

Inferred from electronic annotation. Source: Ensembl

cellular process

Inferred from mutant phenotype PubMed 10570206. Source: MGI

dopamine receptor signaling pathway

Inferred from mutant phenotype PubMed 11248120. Source: MGI

forebrain development

Inferred from electronic annotation. Source: Ensembl

locomotory behavior

Inferred from mutant phenotype PubMed 9501252. Source: MGI

negative regulation of calcium ion transport

Inferred from electronic annotation. Source: Ensembl

neuron projection development

Inferred from electronic annotation. Source: Ensembl

positive regulation of GTPase activity

Inferred from electronic annotation. Source: Ensembl

regulation of heart contraction

Inferred from mutant phenotype PubMed 9050846. Source: MGI

response to cytokine

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to hydrogen peroxide

Inferred from electronic annotation. Source: Ensembl

response to morphine

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextrinsic component of cytoplasmic side of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

heterotrimeric G-protein complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

intracellular

Inferred from direct assay PubMed 16805845. Source: MGI

membrane

Traceable author statement PubMed 8078103. Source: MGI

neuron projection

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionG-protein beta/gamma-subunit complex binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

G-protein coupled serotonin receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

GTP binding

Inferred from mutant phenotype PubMed 11248120. Source: MGI

GTPase activity

Inferred from direct assay Ref.4. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

signal transducer activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha-1 (identifier: P18872-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha-2 (identifier: P18872-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-354: MLFDSICNNK...ANNLRGCGLY → KLFDSICNNK...AKNLRGCGLY

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 354353Guanine nucleotide-binding protein G(o) subunit alpha
PRO_0000203704

Regions

Nucleotide binding40 – 478GTP By similarity
Nucleotide binding176 – 1827GTP By similarity
Nucleotide binding201 – 2055GTP By similarity
Nucleotide binding270 – 2734GTP By similarity

Sites

Metal binding471Magnesium By similarity
Metal binding1821Magnesium By similarity
Binding site3261GTP; via amide nitrogen By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine By similarity
Lipidation31S-palmitoyl cysteine By similarity

Natural variations

Alternative sequence249 – 354106MLFDS…GCGLY → KLFDSICNNKWFTDTSIILF LNKKDIFEEKIKKSPLTICF PEYTGPSAFTEAVAHIQGQY ESKNKSAHKEVYSHVTCATD TNNIQFVFDAVTDVIIAKNL RGCGLY in isoform Alpha-2.
VSP_031251

Secondary structure

.................................................. 354
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B73A84F3BDB09F2C

FASTA35440,085
        10         20         30         40         50         60 
MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV KQMKIIHEDG 

        70         80         90        100        110        120 
FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER KTDSKMVCDV VSRMEDTEPF 

       130        140        150        160        170        180 
SAELLSAMMR LWGDSGIQEC FNRSREYQLN DSAKYYLDSL DRIGAGDYQP TEQDILRTRV 

       190        200        210        220        230        240 
KTTGIVETHF TFKNLHFRLF DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET 

       250        260        270        280        290        300 
TNRMHESLML FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YPGSNTYEDA 

       310        320        330        340        350 
AAYIQTQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG CGLY 

« Hide

Isoform Alpha-2 [UniParc] [UniParc].

Checksum: FCCD80172A673F46
Show »

FASTA35440,036

References

« Hide 'large scale' references
[1]"Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go."
Strathmann M., Wilkie T.M., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 87:6477-6481(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-1 AND ALPHA-2).
Tissue: Brain and Spermatid.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-1).
Strain: C57BL/6.
Tissue: Brain.
[3]Lubec G., Klug S., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 22-32; 36-46; 55-100; 106-143; 146-177; 182-193; 199-206 AND 244-272, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain and Hippocampus.
[4]"RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of galphao."
Traver S., Bidot C., Spassky N., Baltauss T., De Tand M.F., Thomas J.L., Zalc B., Janoueix-Lerosey I., Gunzburg J.D.
Biochem. J. 350:19-29(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RGS14.
[5]"Functional characterization of Galphao signaling through G protein-regulated inducer of neurite outgrowth 1."
Nakata H., Kozasa T.
Mol. Pharmacol. 67:695-702(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M36777 mRNA. Translation: AAA37645.1.
M36778 mRNA. Translation: AAA74566.1.
BC051989 mRNA. Translation: AAH51989.1.
PIRRGMSO1. A36038.
RGMSO2. B36038.
RefSeqNP_001106855.1. NM_001113384.1.
NP_034438.1. NM_010308.3.
UniGeneMm.251445.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90A/C22-354[»]
ProteinModelPortalP18872.
SMRP18872. Positions 35-345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199970. 7 interactions.
DIPDIP-29921N.
IntActP18872. 8 interactions.
MINTMINT-89378.

PTM databases

PhosphoSiteP18872.

Proteomic databases

PaxDbP18872.
PRIDEP18872.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034198; ENSMUSP00000034198; ENSMUSG00000031748. [P18872-1]
ENSMUST00000125716; ENSMUSP00000114144; ENSMUSG00000031748. [P18872-1]
GeneID14681.
KEGGmmu:14681.
UCSCuc009mvk.2. mouse. [P18872-2]
uc009mvl.1. mouse. [P18872-1]

Organism-specific databases

CTD2775.
MGIMGI:95775. Gnao1.

Phylogenomic databases

eggNOGNOG322962.
HOGENOMHOG000038730.
HOVERGENHBG063184.
KOK04534.
OMAKEIYCHQ.
PhylomeDBP18872.
TreeFamTF300673.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

ArrayExpressP18872.
BgeeP18872.
CleanExMM_GNAO1.
GenevestigatorP18872.

Family and domain databases

Gene3D1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10218. PTHR10218. 1 hit.
PfamPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

ChiTaRSGNAO1. mouse.
EvolutionaryTraceP18872.
NextBio286592.
PROP18872.
SOURCESearch...

Entry information

Entry nameGNAO_MOUSE
AccessionPrimary (citable) accession number: P18872
Secondary accession number(s): P18873
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot