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P18872

- GNAO_MOUSE

UniProt

P18872 - GNAO_MOUSE

Protein

Guanine nucleotide-binding protein G(o) subunit alpha

Gene

Gnao1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471MagnesiumBy similarity
    Metal bindingi182 – 1821MagnesiumBy similarity
    Binding sitei326 – 3261GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 478GTPBy similarity
    Nucleotide bindingi176 – 1827GTPBy similarity
    Nucleotide bindingi201 – 2055GTPBy similarity
    Nucleotide bindingi270 – 2734GTPBy similarity

    GO - Molecular functioni

    1. corticotropin-releasing hormone receptor 1 binding Source: RefGenome
    2. G-protein beta/gamma-subunit complex binding Source: RefGenome
    3. G-protein coupled serotonin receptor binding Source: RefGenome
    4. GTPase activity Source: UniProtKB
    5. GTP binding Source: MGI
    6. metal ion binding Source: UniProtKB-KW
    7. mu-type opioid receptor binding Source: RefGenome
    8. protein binding Source: UniProtKB
    9. signal transducer activity Source: RefGenome

    GO - Biological processi

    1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
    2. aging Source: Ensembl
    3. cellular process Source: MGI
    4. dopamine receptor signaling pathway Source: MGI
    5. forebrain development Source: Ensembl
    6. G-protein coupled receptor signaling pathway Source: MGI
    7. locomotory behavior Source: MGI
    8. negative regulation of calcium ion transport Source: Ensembl
    9. neuron projection development Source: Ensembl
    10. positive regulation of GTPase activity Source: Ensembl
    11. regulation of heart contraction Source: MGI
    12. response to cytokine Source: Ensembl
    13. response to drug Source: Ensembl
    14. response to hydrogen peroxide Source: Ensembl
    15. response to morphine Source: Ensembl

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_205726. G-protein activation.
    REACT_221970. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(o) subunit alpha
    Gene namesi
    Name:Gnao1
    Synonyms:Gna0, Gnao
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:95775. Gnao1.

    Subcellular locationi

    Cell membrane 1 Publication

    GO - Cellular componenti

    1. heterotrimeric G-protein complex Source: RefGenome
    2. intracellular Source: MGI
    3. membrane Source: MGI
    4. neuron projection Source: Ensembl
    5. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 354353Guanine nucleotide-binding protein G(o) subunit alphaPRO_0000203704Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate

    Proteomic databases

    MaxQBiP18872.
    PaxDbiP18872.
    PRIDEiP18872.

    PTM databases

    PhosphoSiteiP18872.

    Expressioni

    Gene expression databases

    ArrayExpressiP18872.
    BgeeiP18872.
    CleanExiMM_GNAO1.
    GenevestigatoriP18872.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS14.1 Publication

    Protein-protein interaction databases

    BioGridi199970. 7 interactions.
    DIPiDIP-29921N.
    IntActiP18872. 9 interactions.
    MINTiMINT-89378.

    Structurei

    Secondary structure

    1
    354
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 416
    Turni46 – 494
    Helixi50 – 523
    Helixi63 – 9129
    Helixi100 – 11314
    Turni114 – 1163
    Helixi122 – 13110
    Helixi135 – 1417
    Turni142 – 1454
    Helixi153 – 1575
    Helixi160 – 1634
    Turni172 – 1765
    Beta strandi186 – 1883
    Beta strandi199 – 2013
    Turni206 – 2083
    Helixi209 – 2124
    Helixi214 – 2163
    Beta strandi220 – 2256
    Helixi228 – 2325
    Beta strandi239 – 2424
    Helixi243 – 25513
    Helixi258 – 2603
    Beta strandi263 – 2686
    Helixi272 – 2798
    Helixi284 – 2863
    Helixi297 – 31014
    Helixi329 – 34416

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3C7KX-ray2.90A/C22-354[»]
    ProteinModelPortaliP18872.
    SMRiP18872. Positions 35-345.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18872.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG322962.
    HOGENOMiHOG000038730.
    HOVERGENiHBG063184.
    KOiK04534.
    OMAiKEIYCHQ.
    PhylomeDBiP18872.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Alpha-1 (identifier: P18872-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV    50
    KQMKIIHEDG FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER 100
    KTDSKMVCDV VSRMEDTEPF SAELLSAMMR LWGDSGIQEC FNRSREYQLN 150
    DSAKYYLDSL DRIGAGDYQP TEQDILRTRV KTTGIVETHF TFKNLHFRLF 200
    DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET TNRMHESLML 250
    FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YPGSNTYEDA 300
    AAYIQTQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG 350
    CGLY 354
    Length:354
    Mass (Da):40,085
    Last modified:January 23, 2007 - v3
    Checksum:iB73A84F3BDB09F2C
    GO
    Isoform Alpha-2 (identifier: P18872-2) [UniParc] [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         249-354: MLFDSICNNK...ANNLRGCGLY → KLFDSICNNK...AKNLRGCGLY

    Show »
    Length:354
    Mass (Da):40,036
    Checksum:iFCCD80172A673F46
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei249 – 354106MLFDS…GCGLY → KLFDSICNNKWFTDTSIILF LNKKDIFEEKIKKSPLTICF PEYTGPSAFTEAVAHIQGQY ESKNKSAHKEVYSHVTCATD TNNIQFVFDAVTDVIIAKNL RGCGLY in isoform Alpha-2. 1 PublicationVSP_031251Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36777 mRNA. Translation: AAA37645.1.
    M36778 mRNA. Translation: AAA74566.1.
    BC051989 mRNA. Translation: AAH51989.1.
    CCDSiCCDS22532.1. [P18872-1]
    PIRiA36038. RGMSO1.
    B36038. RGMSO2.
    RefSeqiNP_001106855.1. NM_001113384.1. [P18872-2]
    NP_034438.1. NM_010308.3. [P18872-1]
    UniGeneiMm.251445.

    Genome annotation databases

    EnsembliENSMUST00000034198; ENSMUSP00000034198; ENSMUSG00000031748. [P18872-1]
    ENSMUST00000125716; ENSMUSP00000114144; ENSMUSG00000031748. [P18872-1]
    GeneIDi14681.
    KEGGimmu:14681.
    UCSCiuc009mvk.2. mouse. [P18872-2]
    uc009mvl.1. mouse. [P18872-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36777 mRNA. Translation: AAA37645.1 .
    M36778 mRNA. Translation: AAA74566.1 .
    BC051989 mRNA. Translation: AAH51989.1 .
    CCDSi CCDS22532.1. [P18872-1 ]
    PIRi A36038. RGMSO1.
    B36038. RGMSO2.
    RefSeqi NP_001106855.1. NM_001113384.1. [P18872-2 ]
    NP_034438.1. NM_010308.3. [P18872-1 ]
    UniGenei Mm.251445.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3C7K X-ray 2.90 A/C 22-354 [» ]
    ProteinModelPortali P18872.
    SMRi P18872. Positions 35-345.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199970. 7 interactions.
    DIPi DIP-29921N.
    IntActi P18872. 9 interactions.
    MINTi MINT-89378.

    PTM databases

    PhosphoSitei P18872.

    Proteomic databases

    MaxQBi P18872.
    PaxDbi P18872.
    PRIDEi P18872.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034198 ; ENSMUSP00000034198 ; ENSMUSG00000031748 . [P18872-1 ]
    ENSMUST00000125716 ; ENSMUSP00000114144 ; ENSMUSG00000031748 . [P18872-1 ]
    GeneIDi 14681.
    KEGGi mmu:14681.
    UCSCi uc009mvk.2. mouse. [P18872-2 ]
    uc009mvl.1. mouse. [P18872-1 ]

    Organism-specific databases

    CTDi 2775.
    MGIi MGI:95775. Gnao1.

    Phylogenomic databases

    eggNOGi NOG322962.
    HOGENOMi HOG000038730.
    HOVERGENi HBG063184.
    KOi K04534.
    OMAi KEIYCHQ.
    PhylomeDBi P18872.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_205726. G-protein activation.
    REACT_221970. Ca2+ pathway.

    Miscellaneous databases

    ChiTaRSi GNAO1. mouse.
    EvolutionaryTracei P18872.
    NextBioi 286592.
    PROi P18872.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P18872.
    Bgeei P18872.
    CleanExi MM_GNAO1.
    Genevestigatori P18872.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go."
      Strathmann M., Wilkie T.M., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 87:6477-6481(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-1 AND ALPHA-2).
      Tissue: Brain and Spermatid.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-1).
      Strain: C57BL/6.
      Tissue: Brain.
    3. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 22-32; 36-46; 55-100; 106-143; 146-177; 182-193; 199-206 AND 244-272, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    4. "RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of galphao."
      Traver S., Bidot C., Spassky N., Baltauss T., De Tand M.F., Thomas J.L., Zalc B., Janoueix-Lerosey I., Gunzburg J.D.
      Biochem. J. 350:19-29(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RGS14.
    5. "Functional characterization of Galphao signaling through G protein-regulated inducer of neurite outgrowth 1."
      Nakata H., Kozasa T.
      Mol. Pharmacol. 67:695-702(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGNAO_MOUSE
    AccessioniPrimary (citable) accession number: P18872
    Secondary accession number(s): P18873
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 152 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3