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P18859

- ATP5J_HUMAN

UniProt

P18859 - ATP5J_HUMAN

Protein

ATP synthase-coupling factor 6, mitochondrial

Gene

ATP5J

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.

    GO - Molecular functioni

    1. hydrogen ion transmembrane transporter activity Source: InterPro
    2. transmembrane transporter activity Source: UniProtKB
    3. transporter activity Source: ProtInc

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. cellular metabolic process Source: Reactome
    3. mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
    4. respiratory electron transport chain Source: Reactome
    5. small molecule metabolic process Source: Reactome
    6. substantia nigra development Source: UniProt

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_6759. Formation of ATP by chemiosmotic coupling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP synthase-coupling factor 6, mitochondrial
    Short name:
    ATPase subunit F6
    Gene namesi
    Name:ATP5J
    Synonyms:ATP5A, ATPM
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:847. ATP5J.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Reactome
    2. mitochondrial proton-transporting ATP synthase complex Source: UniProtKB
    3. mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) Source: InterPro
    4. mitochondrion Source: UniProt

    Keywords - Cellular componenti

    CF(0), Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25137.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232Mitochondrion1 PublicationAdd
    BLAST
    Chaini33 – 10876ATP synthase-coupling factor 6, mitochondrialPRO_0000002527Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411N6-acetyllysine1 Publication
    Modified residuei46 – 461N6-acetyllysine1 Publication
    Modified residuei79 – 791N6-acetyllysineBy similarity
    Modified residuei94 – 941N6-acetyllysine; alternateBy similarity
    Modified residuei94 – 941N6-succinyllysine; alternateBy similarity
    Modified residuei99 – 991N6-acetyllysine; alternate1 Publication
    Modified residuei99 – 991N6-succinyllysine; alternateBy similarity
    Modified residuei105 – 1051N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP18859.
    PaxDbiP18859.
    PRIDEiP18859.

    2D gel databases

    SWISS-2DPAGEP18859.

    PTM databases

    PhosphoSiteiP18859.

    Expressioni

    Gene expression databases

    ArrayExpressiP18859.
    BgeeiP18859.
    CleanExiHS_ATP5J.
    GenevestigatoriP18859.

    Organism-specific databases

    HPAiHPA031069.

    Interactioni

    Subunit structurei

    F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF0 seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5F1, ATP5G1, ATP5E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5A1, ATP5B, ATP5D, ATP5C1, ATP5O, ATP5L, USMG5 and MP68 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi107006. 10 interactions.
    IntActiP18859. 6 interactions.
    MINTiMINT-3009016.
    STRINGi9606.ENSP00000389649.

    Structurei

    3D structure databases

    ProteinModelPortaliP18859.
    SMRiP18859. Positions 36-101.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG265662.
    HOGENOMiHOG000261672.
    HOVERGENiHBG062261.
    KOiK02131.
    OMAiDMNTFPN.
    PhylomeDBiP18859.
    TreeFamiTF318998.

    Family and domain databases

    InterProiIPR008387. ATPase_F0-cplx_f6su_mt.
    IPR016349. ATPase_F0-cplx_f6su_mt_subgr.
    [Graphical view]
    PANTHERiPTHR12441. PTHR12441. 1 hit.
    PfamiPF05511. ATP-synt_F6. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002455. ATP_synthase_coupling_factor_6. 1 hit.
    SUPFAMiSSF111357. SSF111357. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P18859-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MILQRLFRFS SVIRSAVSVH LRRNIGVTAV AFNKELDPIQ KLFVDKIREY    50
    KSKRQTSGGP VDASSEYQQE LERELFKLKQ MFGNADMNTF PTFKFEDPKF 100
    EVIEKPQA 108
    Length:108
    Mass (Da):12,588
    Last modified:November 1, 1990 - v1
    Checksum:iEDC1A14F01A10F17
    GO
    Isoform 2 (identifier: P18859-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MHCDGGISM

    Show »
    Length:116
    Mass (Da):13,388
    Checksum:i2E376C23E0F75171
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681Q → H in AAA58630. (PubMed:1830479)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MHCDGGISM in isoform 2. 1 PublicationVSP_046187

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37104 mRNA. Translation: AAA51807.1.
    M73031 mRNA. Translation: AAA58630.1.
    AL110183 mRNA. Translation: CAB53667.1.
    BT007244 mRNA. Translation: AAP35908.1.
    DT217787 mRNA. No translation available.
    AP001694 Genomic DNA. No translation available.
    BC001178 mRNA. Translation: AAH01178.1.
    CCDSiCCDS13574.1. [P18859-1]
    CCDS46637.1. [P18859-2]
    PIRiJT0563.
    RefSeqiNP_001003696.1. NM_001003696.1. [P18859-1]
    NP_001003697.1. NM_001003697.1. [P18859-1]
    NP_001003701.1. NM_001003701.1. [P18859-2]
    NP_001003703.1. NM_001003703.1. [P18859-1]
    NP_001676.2. NM_001685.4. [P18859-1]
    XP_005261049.1. XM_005260992.2. [P18859-1]
    UniGeneiHs.246310.

    Genome annotation databases

    EnsembliENST00000284971; ENSP00000284971; ENSG00000154723. [P18859-1]
    ENST00000400087; ENSP00000382959; ENSG00000154723. [P18859-1]
    ENST00000400090; ENSP00000382962; ENSG00000154723. [P18859-1]
    ENST00000400093; ENSP00000382965; ENSG00000154723. [P18859-1]
    ENST00000400094; ENSP00000382966; ENSG00000154723. [P18859-1]
    ENST00000457143; ENSP00000389649; ENSG00000154723. [P18859-2]
    GeneIDi522.
    KEGGihsa:522.
    UCSCiuc002yls.3. human. [P18859-1]

    Polymorphism databases

    DMDMi114688.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37104 mRNA. Translation: AAA51807.1 .
    M73031 mRNA. Translation: AAA58630.1 .
    AL110183 mRNA. Translation: CAB53667.1 .
    BT007244 mRNA. Translation: AAP35908.1 .
    DT217787 mRNA. No translation available.
    AP001694 Genomic DNA. No translation available.
    BC001178 mRNA. Translation: AAH01178.1 .
    CCDSi CCDS13574.1. [P18859-1 ]
    CCDS46637.1. [P18859-2 ]
    PIRi JT0563.
    RefSeqi NP_001003696.1. NM_001003696.1. [P18859-1 ]
    NP_001003697.1. NM_001003697.1. [P18859-1 ]
    NP_001003701.1. NM_001003701.1. [P18859-2 ]
    NP_001003703.1. NM_001003703.1. [P18859-1 ]
    NP_001676.2. NM_001685.4. [P18859-1 ]
    XP_005261049.1. XM_005260992.2. [P18859-1 ]
    UniGenei Hs.246310.

    3D structure databases

    ProteinModelPortali P18859.
    SMRi P18859. Positions 36-101.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107006. 10 interactions.
    IntActi P18859. 6 interactions.
    MINTi MINT-3009016.
    STRINGi 9606.ENSP00000389649.

    PTM databases

    PhosphoSitei P18859.

    Polymorphism databases

    DMDMi 114688.

    2D gel databases

    SWISS-2DPAGE P18859.

    Proteomic databases

    MaxQBi P18859.
    PaxDbi P18859.
    PRIDEi P18859.

    Protocols and materials databases

    DNASUi 522.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000284971 ; ENSP00000284971 ; ENSG00000154723 . [P18859-1 ]
    ENST00000400087 ; ENSP00000382959 ; ENSG00000154723 . [P18859-1 ]
    ENST00000400090 ; ENSP00000382962 ; ENSG00000154723 . [P18859-1 ]
    ENST00000400093 ; ENSP00000382965 ; ENSG00000154723 . [P18859-1 ]
    ENST00000400094 ; ENSP00000382966 ; ENSG00000154723 . [P18859-1 ]
    ENST00000457143 ; ENSP00000389649 ; ENSG00000154723 . [P18859-2 ]
    GeneIDi 522.
    KEGGi hsa:522.
    UCSCi uc002yls.3. human. [P18859-1 ]

    Organism-specific databases

    CTDi 522.
    GeneCardsi GC21M027088.
    HGNCi HGNC:847. ATP5J.
    HPAi HPA031069.
    MIMi 603152. gene.
    neXtProti NX_P18859.
    PharmGKBi PA25137.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG265662.
    HOGENOMi HOG000261672.
    HOVERGENi HBG062261.
    KOi K02131.
    OMAi DMNTFPN.
    PhylomeDBi P18859.
    TreeFami TF318998.

    Enzyme and pathway databases

    Reactomei REACT_6759. Formation of ATP by chemiosmotic coupling.

    Miscellaneous databases

    GeneWikii ATP5J.
    GenomeRNAii 522.
    NextBioi 2163.
    PROi P18859.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P18859.
    Bgeei P18859.
    CleanExi HS_ATP5J.
    Genevestigatori P18859.

    Family and domain databases

    InterProi IPR008387. ATPase_F0-cplx_f6su_mt.
    IPR016349. ATPase_F0-cplx_f6su_mt_subgr.
    [Graphical view ]
    PANTHERi PTHR12441. PTHR12441. 1 hit.
    Pfami PF05511. ATP-synt_F6. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002455. ATP_synthase_coupling_factor_6. 1 hit.
    SUPFAMi SSF111357. SSF111357. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Human mitochondrial ATP synthase: cloning cDNA for the nuclear-encoded precursor of coupling factor 6."
      Javed A.A., Ogata K., Sanadi D.R.
      Gene 97:307-310(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Molecular cloning of cDNA for the import precursor of human coupling factor 6 of H(+)-ATP synthase in mitochondria."
      Higuti T., Tsurumi C., Kawamura Y., Tsujita H., Osaka F., Yoshihara Y., Tani I., Tanaka K., Ichihara A.
      Biochem. Biophys. Res. Commun. 178:793-799(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Kidney.
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Identification of genes related to Parkinson's disease using expressed sequence tags."
      Kim J.M., Lee K.H., Jeon Y.J., Oh J.H., Jeong S.Y., Song I.S., Kim J.M., Lee D.S., Kim N.S.
      DNA Res. 13:275-286(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. "The DNA sequence of human chromosome 21."
      Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
      , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
      Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Eye.
    8. "Human liver protein map: a reference database established by microsequencing and gel comparison."
      Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
      Electrophoresis 13:992-1001(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 33-43.
      Tissue: Liver.
    9. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-41; LYS-46; LYS-99 AND LYS-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiATP5J_HUMAN
    AccessioniPrimary (citable) accession number: P18859
    Secondary accession number(s): J3KQ83
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 148 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3