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P18852

- GBG_YEAST

UniProt

P18852 - GBG_YEAST

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Protein

Guanine nucleotide-binding protein subunit gamma

Gene
STE18, YJR086W, J1866
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Implicated in the pheromone A- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response.

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. signal transducer activity Source: SGD

GO - Biological processi

  1. heterotrimeric G-protein complex cycle Source: SGD
  2. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Pheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-31714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit gamma
Gene namesi
Name:STE18
Ordered Locus Names:YJR086W
ORF Names:J1866
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR086w.
SGDiS000003846. STE18.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. heterotrimeric G-protein complex Source: SGD
  3. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi106 – 1061C → S: Partial loss of function. 1 Publication
Mutagenesisi107 – 1071C → X: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 107107Guanine nucleotide-binding protein subunit gammaPRO_0000194812Add
BLAST
Propeptidei108 – 1103Removed in mature formPRO_0000396775

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi106 – 1061S-palmitoyl cysteine2 Publications
Modified residuei107 – 1071Cysteine methyl ester1 Publication
Lipidationi107 – 1071S-farnesyl cysteine2 Publications

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

MaxQBiP18852.
PaxDbiP18852.

Expressioni

Gene expression databases

GenevestigatoriP18852.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. The beta-gamma subunit complex (STE4-STE18 complex) interacts with PLP1 and PLP2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
STE4P188513EBI-7397,EBI-7390

Protein-protein interaction databases

BioGridi33841. 12 interactions.
DIPiDIP-314N.
IntActiP18852. 2 interactions.
MINTiMINT-394213.
STRINGi4932.YJR086W.

Structurei

Secondary structure

1
110
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 4615
Helixi53 – 6614

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SCGmodel-G1-110[»]
ProteinModelPortaliP18852.
SMRiP18852. Positions 38-72.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 2212Gln-richAdd
BLAST
Compositional biasi96 – 1049Asn/Ser-rich

Sequence similaritiesi

Belongs to the G protein gamma family.

Phylogenomic databases

eggNOGiNOG39072.
HOGENOMiHOG000210889.
KOiK07973.
OMAiHEETSAC.
OrthoDBiEOG7D5B1T.

Family and domain databases

InterProiIPR015898. G-protein_gamma-like_dom.
[Graphical view]
PfamiPF00631. G-gamma. 1 hit.
[Graphical view]
SMARTiSM00224. GGL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18852-1 [UniParc]FASTAAdd to Basket

« Hide

MTSVQNSPRL QQPQEQQQQQ QQLSLKIKQL KLKRINELNN KLRKELSRER    50
ITASNACLTI INYTSNTKDY TLPELWGYPV AGSNHFIEGL KNAQKNSQMS 100
NSNSVCCTLM 110
Length:110
Mass (Da):12,625
Last modified:November 1, 1990 - v1
Checksum:i6A78E946DA5059FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23983 Genomic DNA. Translation: AAA35110.1.
L47993 Genomic DNA. Translation: AAB39309.1.
Z49586 Genomic DNA. Translation: CAA89613.1.
AY557888 Genomic DNA. Translation: AAS56214.1.
BK006943 Genomic DNA. Translation: DAA08871.1.
PIRiB30102.
RefSeqiNP_012619.1. NM_001181743.1.

Genome annotation databases

EnsemblFungiiYJR086W; YJR086W; YJR086W.
GeneIDi853548.
KEGGisce:YJR086W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23983 Genomic DNA. Translation: AAA35110.1 .
L47993 Genomic DNA. Translation: AAB39309.1 .
Z49586 Genomic DNA. Translation: CAA89613.1 .
AY557888 Genomic DNA. Translation: AAS56214.1 .
BK006943 Genomic DNA. Translation: DAA08871.1 .
PIRi B30102.
RefSeqi NP_012619.1. NM_001181743.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SCG model - G 1-110 [» ]
ProteinModelPortali P18852.
SMRi P18852. Positions 38-72.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33841. 12 interactions.
DIPi DIP-314N.
IntActi P18852. 2 interactions.
MINTi MINT-394213.
STRINGi 4932.YJR086W.

Proteomic databases

MaxQBi P18852.
PaxDbi P18852.

Protocols and materials databases

DNASUi 853548.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR086W ; YJR086W ; YJR086W .
GeneIDi 853548.
KEGGi sce:YJR086W.

Organism-specific databases

CYGDi YJR086w.
SGDi S000003846. STE18.

Phylogenomic databases

eggNOGi NOG39072.
HOGENOMi HOG000210889.
KOi K07973.
OMAi HEETSAC.
OrthoDBi EOG7D5B1T.

Enzyme and pathway databases

BioCyci YEAST:G3O-31714-MONOMER.

Miscellaneous databases

NextBioi 974278.

Gene expression databases

Genevestigatori P18852.

Family and domain databases

InterProi IPR015898. G-protein_gamma-like_dom.
[Graphical view ]
Pfami PF00631. G-gamma. 1 hit.
[Graphical view ]
SMARTi SM00224. GGL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein."
    Whiteway M., Hougan L., Dignard D., Thomas D.Y., Bell L., Saari G.C., Grant F.J., O'Hara P., Mackay V.L.
    Cell 56:467-477(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Site-directed mutations altering the CAAX box of Ste18, the yeast pheromone-response pathway G gamma subunit."
    Whiteway M.S., Thomas D.Y.
    Genetics 137:967-976(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-107.
  7. "Dual lipid modification of the yeast gamma subunit Ste18p determines membrane localization of Gbetagamma."
    Hirschman J.E., Jenness D.D.
    Mol. Cell. Biol. 19:7705-7711(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-107, METHYLATION AT CYS-107, PALMITOYLATION AT CYS-106, MUTAGENESIS OF CYS-106.
  8. "Dual lipid modification motifs in G(alpha) and G(gamma) subunits are required for full activity of the pheromone response pathway in Saccharomyces cerevisiae."
    Manahan C.L., Patnana M., Blumer K.J., Linder M.E.
    Mol. Biol. Cell 11:957-968(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-107, PALMITOYLATION AT CYS-106.
  9. "Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast."
    Flanary P.L., DiBello P.R., Estrada P., Dohlman H.G.
    J. Biol. Chem. 275:18462-18469(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLP1 AND PLP2.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Gaitatzes C.G., Neer E.J., Smith T.F.
    Submitted (FEB-1998) to the PDB data bank
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiGBG_YEAST
AccessioniPrimary (citable) accession number: P18852
Secondary accession number(s): D6VWQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5550 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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