Guanine nucleotide-binding protein subunit gamma precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P18852 (GBG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein subunit gamma

Gene names

Name:	STE18
Ordered Locus Names:	YJR086W
ORF Names:	J1866

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in the pheromone A- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response.
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma. The beta-gamma subunit complex (STE4-STE18 complex) interacts with PLP1 and PLP2. Ref.9
Subcellular location	Membrane; Lipid-anchor.
Miscellaneous	Present with 5550 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the G protein gamma family.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Membrane
Molecular function	Transducer
PTM	Lipoprotein Methylation Palmitate Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heterotrimeric G-protein complex cycle Inferred from mutant phenotype PubMed 2107073. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype PubMed 3151178. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 8995254. Source: SGD heterotrimeric G-protein complex Inferred from direct assay PubMed 8995254. Source: SGD
Molecular_function	signal transducer activity Inferred from mutant phenotype PubMed 2107073. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STE4	P18851	3	EBI-7397,EBI-7390

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 107

107

Guanine nucleotide-binding protein subunit gamma

PRO_0000194812

Propeptide

108 – 110

Removed in mature form

PRO_0000396775

Regions

Compositional bias

11 – 22

Gln-rich

Compositional bias

96 – 104

Asn/Ser-rich

Amino acid modifications

Modified residue

107

Cysteine methyl ester

Lipidation

106

S-palmitoyl cysteine Ref.7 Ref.8

Lipidation

107

S-farnesyl cysteine Ref.7 Ref.8

Experimental info

Mutagenesis

106

C → S: Partial loss of function. Ref.7

Mutagenesis

107

C → X: Loss of function. Ref.6

Secondary structure

110

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P18852 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 6A78E946DA5059FA
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FASTA

110

12,625

        10         20         30         40         50         60 
MTSVQNSPRL QQPQEQQQQQ QQLSLKIKQL KLKRINELNN KLRKELSRER ITASNACLTI 

        70         80         90        100        110 
INYTSNTKDY TLPELWGYPV AGSNHFIEGL KNAQKNSQMS NSNSVCCTLM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein." Whiteway M., Hougan L., Dignard D., Thomas D.Y., Bell L., Saari G.C., Grant F.J., O'Hara P., Mackay V.L. Cell 56:467-477(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Site-directed mutations altering the CAAX box of Ste18, the yeast pheromone-response pathway G gamma subunit." Whiteway M.S., Thomas D.Y. Genetics 137:967-976(1994) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF CYS-107.
[7]	"Dual lipid modification of the yeast gamma subunit Ste18p determines membrane localization of Gbetagamma." Hirschman J.E., Jenness D.D. Mol. Cell. Biol. 19:7705-7711(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-107, PALMITOYLATION AT CYS-106, MUTAGENESIS OF CYS-106.
[8]	"Dual lipid modification motifs in G(alpha) and G(gamma) subunits are required for full activity of the pheromone response pathway in Saccharomyces cerevisiae." Manahan C.L., Patnana M., Blumer K.J., Linder M.E. Mol. Biol. Cell 11:957-968(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-107, PALMITOYLATION AT CYS-106.
[9]	"Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast." Flanary P.L., DiBello P.R., Estrada P., Dohlman H.G. J. Biol. Chem. 275:18462-18469(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PLP1 AND PLP2.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	Gaitatzes C.G., Neer E.J., Smith T.F. Submitted (FEB-1998) to the PDB data bank Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M23983 Genomic DNA. Translation: AAA35110.1.
L47993 Genomic DNA. Translation: AAB39309.1.
Z49586 Genomic DNA. Translation: CAA89613.1.
AY557888 Genomic DNA. Translation: AAS56214.1.
BK006943 Genomic DNA. Translation: DAA08871.1.

PIR

B30102.

RefSeq

NP_012619.1. NM_001181743.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SCG	model	-	G	1-110	[»]

ProteinModelPortal

P18852.

SMR

P18852. Positions 37-88.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-314N.

IntAct

P18852. 1 interaction.

MINT

MINT-394213.

STRING

4932.YJR086W.

Proteomic databases

PaxDb

P18852.

Protocols and materials databases

DNASU

853548.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJR086W; YJR086W; YJR086W.

GeneID

853548.

KEGG

sce:YJR086W.

Organism-specific databases

CYGD

YJR086w.

SGD

S000003846. STE18.

Phylogenomic databases

eggNOG

NOG39072.

HOGENOM

HOG000210889.

K07973.

OMA

ACLTIIN.

OrthoDB

EOG42C1KB.

Enzyme and pathway databases

BioCyc

YEAST:G3O-31714-MONOMER.

Gene expression databases

Genevestigator

P18852.

GermOnline

YJR086W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR015898. G-protein_gamma-like_dom.
[Graphical view]

Pfam

PF00631. G-gamma. 1 hit.
[Graphical view]

SMART

SM00224. GGL. 1 hit.
[Graphical view]

PROSITE

PS50058. G_PROTEIN_GAMMA. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

974278.

Entry information

Entry name

GBG_YEAST

Accession

Primary (citable) accession number: P18852
Secondary accession number(s): D6VWQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 29, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents