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Protein

Guanine nucleotide-binding protein subunit beta

Gene

STE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in the a- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  1. signal transducer activity Source: SGD

GO - Biological processi

  1. chemotropism Source: SGD
  2. heterotrimeric G-protein complex cycle Source: SGD
  3. invasive growth in response to glucose limitation Source: SGD
  4. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  5. regulation of transposition, RNA-mediated Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Pheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-33714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta
Gene namesi
Name:STE4
Ordered Locus Names:YOR212W
ORF Names:YOR50-2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR212w.
SGDiS000005738. STE4.

Subcellular locationi

GO - Cellular componenti

  1. heterotrimeric G-protein complex Source: SGD
  2. mating projection Source: SGD
  3. plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Guanine nucleotide-binding protein subunit betaPRO_0000127720Add
BLAST

Proteomic databases

MaxQBiP18851.
PaxDbiP18851.
PeptideAtlasiP18851.

Expressioni

Gene expression databases

GenevestigatoriP18851.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. The beta-gamma subunit complex (STE4-STE18 complex) interacts with PLP1 and PLP2. Interacts with SYG1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AKR1P390103EBI-7390,EBI-2421
GPA1P085398EBI-7390,EBI-7376
STE18P188523EBI-7390,EBI-7397
STE20Q034973EBI-7390,EBI-18285
STE5P329172EBI-7390,EBI-18373

Protein-protein interaction databases

BioGridi34607. 69 interactions.
DIPiDIP-954N.
IntActiP18851. 17 interactions.
MINTiMINT-364784.
STRINGi4932.YOR212W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SCGmodel-B1-423[»]
ProteinModelPortaliP18851.
SMRiP18851. Positions 37-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati90 – 12031WD 1Add
BLAST
Repeati132 – 16231WD 2Add
BLAST
Repeati179 – 20830WD 3Add
BLAST
Repeati220 – 25637WD 4Add
BLAST
Repeati268 – 29831WD 5Add
BLAST
Repeati348 – 37730WD 6Add
BLAST
Repeati389 – 41931WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
InParanoidiP18851.
KOiK04536.
OMAiITSSGDC.
OrthoDBiEOG7JT75V.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18851-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH
60 70 80 90 100
AQINKAKHKI QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW
110 120 130 140 150
SRDSKRILSA SQDGFMLIWD SASGLKQNAI PLDSQWVLSC AISPSSTLVA
160 170 180 190 200
SAGLNNNCTI YRVSKENRVA QNVASIFKGH TCYISDIEFT DNAHILTASG
210 220 230 240 250
DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS NTFASCGSDG
260 270 280 290 300
YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR
310 320 330 340 350
SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ
360 370 380 390 400
GVVSLDFSAS GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS
410 420
PDGLAVCTGS WDSTMKIWSP GYQ
Length:423
Mass (Da):46,581
Last modified:February 1, 1996 - v2
Checksum:i4B0E26040AAA83E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331D → E in AAA35114. (PubMed:2536595)Curated
Sequence conflicti236 – 2361S → L in AAA35114. (PubMed:2536595)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23982 Genomic DNA. Translation: AAA35114.1.
X92441 Genomic DNA. Translation: CAA63175.1.
Z75120 Genomic DNA. Translation: CAA99427.1.
BK006948 Genomic DNA. Translation: DAA10984.1.
PIRiS60939.
RefSeqiNP_014855.3. NM_001183631.3.

Genome annotation databases

EnsemblFungiiYOR212W; YOR212W; YOR212W.
GeneIDi854387.
KEGGisce:YOR212W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23982 Genomic DNA. Translation: AAA35114.1.
X92441 Genomic DNA. Translation: CAA63175.1.
Z75120 Genomic DNA. Translation: CAA99427.1.
BK006948 Genomic DNA. Translation: DAA10984.1.
PIRiS60939.
RefSeqiNP_014855.3. NM_001183631.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SCGmodel-B1-423[»]
ProteinModelPortaliP18851.
SMRiP18851. Positions 37-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34607. 69 interactions.
DIPiDIP-954N.
IntActiP18851. 17 interactions.
MINTiMINT-364784.
STRINGi4932.YOR212W.

Proteomic databases

MaxQBiP18851.
PaxDbiP18851.
PeptideAtlasiP18851.

Protocols and materials databases

DNASUi854387.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR212W; YOR212W; YOR212W.
GeneIDi854387.
KEGGisce:YOR212W.

Organism-specific databases

CYGDiYOR212w.
SGDiS000005738. STE4.

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
InParanoidiP18851.
KOiK04536.
OMAiITSSGDC.
OrthoDBiEOG7JT75V.

Enzyme and pathway databases

BioCyciYEAST:G3O-33714-MONOMER.

Miscellaneous databases

NextBioi976538.

Gene expression databases

GenevestigatoriP18851.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein."
    Whiteway M., Hougan L., Dignard D., Thomas D.Y., Bell L., Saari G.C., Grant F.J., O'Hara P., Mackay V.L.
    Cell 56:467-477(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
    Galisson F., Dujon B.
    Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit."
    Spain B.H., Koo D., Ramakrishnan M., Dzudzor B., Colicelli J.
    J. Biol. Chem. 270:25435-25444(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SYG1.
  6. "Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast."
    Flanary P.L., DiBello P.R., Estrada P., Dohlman H.G.
    J. Biol. Chem. 275:18462-18469(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLP1 AND PLP2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Gaitatzes C.G., Neer E.J., Smith T.F.
    Submitted (FEB-1998) to the PDB data bank
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiGBB_YEAST
AccessioniPrimary (citable) accession number: P18851
Secondary accession number(s): D6W2R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.