Skip Header

Contribute Send feedback
Read comments (?) or add your own

P18851 (GBB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein subunit beta
Gene names
Name:STE4
Ordered Locus Names:YOR212W
ORF Names:YOR50-2
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the a- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit structure

G proteins are composed of 3 units, alpha, beta and gamma. The beta-gamma subunit complex (STE4-STE18 complex) interacts with PLP1 and PLP2. Interacts with SYG1. Ref.5 Ref.6

Miscellaneous

Present with 2050 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GPA1P085398EBI-7390,EBI-7376
STE18P188523EBI-7390,EBI-7397
STE20Q034973EBI-7390,EBI-18285
STE5P329172EBI-7390,EBI-18373

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Guanine nucleotide-binding protein subunit beta
PRO_0000127720

Regions

Repeat90 – 12031WD 1
Repeat132 – 16231WD 2
Repeat179 – 20830WD 3
Repeat220 – 25637WD 4
Repeat268 – 29831WD 5
Repeat348 – 37730WD 6
Repeat389 – 41931WD 7

Amino acid modifications

Modified residue3181Phosphothreonine Ref.8

Experimental info

Sequence conflict331D → E in AAA35114. Ref.1
Sequence conflict2361S → L in AAA35114. Ref.1

Secondary structure

1 ........................................................................................... 423
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18851 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 4B0E26040AAA83E5

FASTA42346,581
        10         20         30         40         50         60 
MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH AQINKAKHKI 

        70         80         90        100        110        120 
QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW SRDSKRILSA SQDGFMLIWD 

       130        140        150        160        170        180 
SASGLKQNAI PLDSQWVLSC AISPSSTLVA SAGLNNNCTI YRVSKENRVA QNVASIFKGH 

       190        200        210        220        230        240 
TCYISDIEFT DNAHILTASG DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS 

       250        260        270        280        290        300 
NTFASCGSDG YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR 

       310        320        330        340        350        360 
SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ GVVSLDFSAS 

       370        380        390        400        410        420 
GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS PDGLAVCTGS WDSTMKIWSP 


GYQ

« Hide

References

« Hide 'large scale' references
[1]"The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein."
Whiteway M., Hougan L., Dignard D., Thomas D.Y., Bell L., Saari G.C., Grant F.J., O'Hara P., Mackay V.L.
Cell 56:467-477(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
Galisson F., Dujon B.
Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit."
Spain B.H., Koo D., Ramakrishnan M., Dzudzor B., Colicelli J.
J. Biol. Chem. 270:25435-25444(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SYG1.
[6]"Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast."
Flanary P.L., DiBello P.R., Estrada P., Dohlman H.G.
J. Biol. Chem. 275:18462-18469(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PLP1 AND PLP2.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-318, MASS SPECTROMETRY.
[9]Gaitatzes C.G., Neer E.J., Smith T.F.
Submitted (FEB-1998) to the PDB data bank
Cited for: 3D-STRUCTURE MODELING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M23982 Genomic DNA. Translation: AAA35114.1.
X92441 Genomic DNA. Translation: CAA63175.1.
Z75120 Genomic DNA. Translation: CAA99427.1.
BK006948 Genomic DNA. Translation: DAA10984.1.
PIRS60939.
RefSeqNP_014855.3. NM_001183631.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SCGmodel-B1-423[»]
ProteinModelPortalP18851.
SMRP18851. Positions 37-419.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-954N.
IntActP18851. 28 interactions.
MINTMINT-364784.
STRING4932.YOR212W.

Proteomic databases

PaxDbP18851.
PeptideAtlasP18851.

Protocols and materials databases

DNASU854387.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR212W; YOR212W; YOR212W.
GeneID854387.
KEGGsce:YOR212W.

Organism-specific databases

CYGDYOR212w.
SGDS000005738. STE4.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00550000074331.
HOGENOMHOG000176356.
KOK07972.
OMAYTHDNIL.
OrthoDBEOG454D7D.

Enzyme and pathway databases

BioCycYEAST:G3O-33714-MONOMER.

Gene expression databases

GenevestigatorP18851.
GermOnlineYOR212W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 5 hits.
[Graphical view]
PIRSFPIRSF002394. GN-bd_beta. 1 hit.
PRINTSPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976538.

Entry information

Entry nameGBB_YEAST
AccessionPrimary (citable) accession number: P18851
Secondary accession number(s): D6W2R8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: May 29, 2013
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents