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P18851

- GBB_YEAST

UniProt

P18851 - GBB_YEAST

Protein

Guanine nucleotide-binding protein subunit beta

Gene

STE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Implicated in the a- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. signal transducer activity Source: SGD

    GO - Biological processi

    1. heterotrimeric G-protein complex cycle Source: SGD
    2. invasive growth in response to glucose limitation Source: SGD
    3. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
    4. regulation of transposition, RNA-mediated Source: SGD

    Keywords - Molecular functioni

    Transducer

    Keywords - Biological processi

    Pheromone response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33714-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein subunit beta
    Gene namesi
    Name:STE4
    Ordered Locus Names:YOR212W
    ORF Names:YOR50-2
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR212w.
    SGDiS000005738. STE4.

    Subcellular locationi

    GO - Cellular componenti

    1. heterotrimeric G-protein complex Source: SGD
    2. mating projection Source: SGD
    3. plasma membrane Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423Guanine nucleotide-binding protein subunit betaPRO_0000127720Add
    BLAST

    Proteomic databases

    MaxQBiP18851.
    PaxDbiP18851.
    PeptideAtlasiP18851.

    Expressioni

    Gene expression databases

    GenevestigatoriP18851.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. The beta-gamma subunit complex (STE4-STE18 complex) interacts with PLP1 and PLP2. Interacts with SYG1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AKR1P390103EBI-7390,EBI-2421
    GPA1P085398EBI-7390,EBI-7376
    STE18P188523EBI-7390,EBI-7397
    STE20Q034973EBI-7390,EBI-18285
    STE5P329172EBI-7390,EBI-18373

    Protein-protein interaction databases

    BioGridi34607. 68 interactions.
    DIPiDIP-954N.
    IntActiP18851. 17 interactions.
    MINTiMINT-364784.
    STRINGi4932.YOR212W.

    Structurei

    Secondary structure

    1
    423
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi40 – 6223
    Helixi67 – 715
    Turni72 – 732
    Beta strandi84 – 874
    Turni102 – 1032
    Beta strandi106 – 1083
    Turni112 – 1132
    Beta strandi115 – 1206
    Turni121 – 1233
    Beta strandi126 – 1316
    Beta strandi137 – 1426
    Turni144 – 1452
    Beta strandi148 – 1536
    Turni154 – 1552
    Beta strandi158 – 1625
    Turni163 – 1642
    Turni166 – 1672
    Beta strandi175 – 1773
    Beta strandi184 – 1918
    Turni192 – 1932
    Beta strandi194 – 1996
    Turni200 – 2023
    Beta strandi203 – 2086
    Helixi209 – 2113
    Turni212 – 2121
    Beta strandi213 – 2197
    Beta strandi225 – 2295
    Beta strandi242 – 2476
    Beta strandi251 – 2566
    Beta strandi262 – 2676
    Beta strandi275 – 2784
    Turni280 – 2812
    Beta strandi284 – 2885
    Beta strandi294 – 2985
    Turni299 – 3024
    Beta strandi303 – 3086
    Turni310 – 3123
    Helixi315 – 3173
    Turni319 – 3224
    Helixi323 – 3264
    Helixi329 – 3324
    Helixi334 – 3407
    Beta strandi355 – 3573
    Turni359 – 3602
    Beta strandi363 – 3664
    Beta strandi374 – 3774
    Turni378 – 3803
    Beta strandi383 – 3853
    Beta strandi394 – 3996
    Turni401 – 4022
    Beta strandi406 – 4105
    Turni411 – 4122
    Beta strandi415 – 4184

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SCGmodel-B1-423[»]
    ProteinModelPortaliP18851.
    SMRiP18851. Positions 37-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati90 – 12031WD 1Add
    BLAST
    Repeati132 – 16231WD 2Add
    BLAST
    Repeati179 – 20830WD 3Add
    BLAST
    Repeati220 – 25637WD 4Add
    BLAST
    Repeati268 – 29831WD 5Add
    BLAST
    Repeati348 – 37730WD 6Add
    BLAST
    Repeati389 – 41931WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat G protein beta family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115438.
    HOGENOMiHOG000176356.
    KOiK04536.
    OMAiATSFTGH.
    OrthoDBiEOG7JT75V.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 5 hits.
    [Graphical view]
    PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
    PRINTSiPR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18851-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH    50
    AQINKAKHKI QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW 100
    SRDSKRILSA SQDGFMLIWD SASGLKQNAI PLDSQWVLSC AISPSSTLVA 150
    SAGLNNNCTI YRVSKENRVA QNVASIFKGH TCYISDIEFT DNAHILTASG 200
    DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS NTFASCGSDG 250
    YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR 300
    SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ 350
    GVVSLDFSAS GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS 400
    PDGLAVCTGS WDSTMKIWSP GYQ 423
    Length:423
    Mass (Da):46,581
    Last modified:February 1, 1996 - v2
    Checksum:i4B0E26040AAA83E5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331D → E in AAA35114. (PubMed:2536595)Curated
    Sequence conflicti236 – 2361S → L in AAA35114. (PubMed:2536595)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23982 Genomic DNA. Translation: AAA35114.1.
    X92441 Genomic DNA. Translation: CAA63175.1.
    Z75120 Genomic DNA. Translation: CAA99427.1.
    BK006948 Genomic DNA. Translation: DAA10984.1.
    PIRiS60939.
    RefSeqiNP_014855.3. NM_001183631.3.

    Genome annotation databases

    EnsemblFungiiYOR212W; YOR212W; YOR212W.
    GeneIDi854387.
    KEGGisce:YOR212W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23982 Genomic DNA. Translation: AAA35114.1 .
    X92441 Genomic DNA. Translation: CAA63175.1 .
    Z75120 Genomic DNA. Translation: CAA99427.1 .
    BK006948 Genomic DNA. Translation: DAA10984.1 .
    PIRi S60939.
    RefSeqi NP_014855.3. NM_001183631.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SCG model - B 1-423 [» ]
    ProteinModelPortali P18851.
    SMRi P18851. Positions 37-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34607. 68 interactions.
    DIPi DIP-954N.
    IntActi P18851. 17 interactions.
    MINTi MINT-364784.
    STRINGi 4932.YOR212W.

    Proteomic databases

    MaxQBi P18851.
    PaxDbi P18851.
    PeptideAtlasi P18851.

    Protocols and materials databases

    DNASUi 854387.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR212W ; YOR212W ; YOR212W .
    GeneIDi 854387.
    KEGGi sce:YOR212W.

    Organism-specific databases

    CYGDi YOR212w.
    SGDi S000005738. STE4.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115438.
    HOGENOMi HOG000176356.
    KOi K04536.
    OMAi ATSFTGH.
    OrthoDBi EOG7JT75V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33714-MONOMER.

    Miscellaneous databases

    NextBioi 976538.

    Gene expression databases

    Genevestigatori P18851.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 5 hits.
    [Graphical view ]
    PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
    PRINTSi PR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein."
      Whiteway M., Hougan L., Dignard D., Thomas D.Y., Bell L., Saari G.C., Grant F.J., O'Hara P., Mackay V.L.
      Cell 56:467-477(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
      Galisson F., Dujon B.
      Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit."
      Spain B.H., Koo D., Ramakrishnan M., Dzudzor B., Colicelli J.
      J. Biol. Chem. 270:25435-25444(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYG1.
    6. "Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast."
      Flanary P.L., DiBello P.R., Estrada P., Dohlman H.G.
      J. Biol. Chem. 275:18462-18469(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PLP1 AND PLP2.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Gaitatzes C.G., Neer E.J., Smith T.F.
      Submitted (FEB-1998) to the PDB data bank
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiGBB_YEAST
    AccessioniPrimary (citable) accession number: P18851
    Secondary accession number(s): D6W2R8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2050 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3