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P18851

- GBB_YEAST

UniProt

P18851 - GBB_YEAST

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Protein
Guanine nucleotide-binding protein subunit beta
Gene
STE4, YOR212W, YOR50-2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Implicated in the a- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. signal transducer activity Source: SGD

GO - Biological processi

  1. heterotrimeric G-protein complex cycle Source: SGD
  2. invasive growth in response to glucose limitation Source: SGD
  3. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  4. regulation of transposition, RNA-mediated Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Pheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-33714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta
Gene namesi
Name:STE4
Ordered Locus Names:YOR212W
ORF Names:YOR50-2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR212w.
SGDiS000005738. STE4.

Subcellular locationi

GO - Cellular componenti

  1. heterotrimeric G-protein complex Source: SGD
  2. mating projection Source: SGD
  3. plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Guanine nucleotide-binding protein subunit beta
PRO_0000127720Add
BLAST

Proteomic databases

MaxQBiP18851.
PaxDbiP18851.
PeptideAtlasiP18851.

Expressioni

Gene expression databases

GenevestigatoriP18851.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. The beta-gamma subunit complex (STE4-STE18 complex) interacts with PLP1 and PLP2. Interacts with SYG1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AKR1P390103EBI-7390,EBI-2421
GPA1P085398EBI-7390,EBI-7376
STE18P188523EBI-7390,EBI-7397
STE20Q034973EBI-7390,EBI-18285
STE5P329172EBI-7390,EBI-18373

Protein-protein interaction databases

BioGridi34607. 68 interactions.
DIPiDIP-954N.
IntActiP18851. 17 interactions.
MINTiMINT-364784.
STRINGi4932.YOR212W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 6223
Helixi67 – 715
Turni72 – 732
Beta strandi84 – 874
Turni102 – 1032
Beta strandi106 – 1083
Turni112 – 1132
Beta strandi115 – 1206
Turni121 – 1233
Beta strandi126 – 1316
Beta strandi137 – 1426
Turni144 – 1452
Beta strandi148 – 1536
Turni154 – 1552
Beta strandi158 – 1625
Turni163 – 1642
Turni166 – 1672
Beta strandi175 – 1773
Beta strandi184 – 1918
Turni192 – 1932
Beta strandi194 – 1996
Turni200 – 2023
Beta strandi203 – 2086
Helixi209 – 2113
Turni212 – 2121
Beta strandi213 – 2197
Beta strandi225 – 2295
Beta strandi242 – 2476
Beta strandi251 – 2566
Beta strandi262 – 2676
Beta strandi275 – 2784
Turni280 – 2812
Beta strandi284 – 2885
Beta strandi294 – 2985
Turni299 – 3024
Beta strandi303 – 3086
Turni310 – 3123
Helixi315 – 3173
Turni319 – 3224
Helixi323 – 3264
Helixi329 – 3324
Helixi334 – 3407
Beta strandi355 – 3573
Turni359 – 3602
Beta strandi363 – 3664
Beta strandi374 – 3774
Turni378 – 3803
Beta strandi383 – 3853
Beta strandi394 – 3996
Turni401 – 4022
Beta strandi406 – 4105
Turni411 – 4122
Beta strandi415 – 4184

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SCGmodel-B1-423[»]
ProteinModelPortaliP18851.
SMRiP18851. Positions 37-419.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati90 – 12031WD 1
Add
BLAST
Repeati132 – 16231WD 2
Add
BLAST
Repeati179 – 20830WD 3
Add
BLAST
Repeati220 – 25637WD 4
Add
BLAST
Repeati268 – 29831WD 5
Add
BLAST
Repeati348 – 37730WD 6
Add
BLAST
Repeati389 – 41931WD 7
Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00740000115438.
HOGENOMiHOG000176356.
KOiK04536.
OMAiATSFTGH.
OrthoDBiEOG7JT75V.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18851-1 [UniParc]FASTAAdd to Basket

« Hide

MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH    50
AQINKAKHKI QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW 100
SRDSKRILSA SQDGFMLIWD SASGLKQNAI PLDSQWVLSC AISPSSTLVA 150
SAGLNNNCTI YRVSKENRVA QNVASIFKGH TCYISDIEFT DNAHILTASG 200
DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS NTFASCGSDG 250
YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR 300
SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ 350
GVVSLDFSAS GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS 400
PDGLAVCTGS WDSTMKIWSP GYQ 423
Length:423
Mass (Da):46,581
Last modified:February 1, 1996 - v2
Checksum:i4B0E26040AAA83E5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331D → E in AAA35114. 1 Publication
Sequence conflicti236 – 2361S → L in AAA35114. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23982 Genomic DNA. Translation: AAA35114.1.
X92441 Genomic DNA. Translation: CAA63175.1.
Z75120 Genomic DNA. Translation: CAA99427.1.
BK006948 Genomic DNA. Translation: DAA10984.1.
PIRiS60939.
RefSeqiNP_014855.3. NM_001183631.3.

Genome annotation databases

EnsemblFungiiYOR212W; YOR212W; YOR212W.
GeneIDi854387.
KEGGisce:YOR212W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23982 Genomic DNA. Translation: AAA35114.1 .
X92441 Genomic DNA. Translation: CAA63175.1 .
Z75120 Genomic DNA. Translation: CAA99427.1 .
BK006948 Genomic DNA. Translation: DAA10984.1 .
PIRi S60939.
RefSeqi NP_014855.3. NM_001183631.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SCG model - B 1-423 [» ]
ProteinModelPortali P18851.
SMRi P18851. Positions 37-419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34607. 68 interactions.
DIPi DIP-954N.
IntActi P18851. 17 interactions.
MINTi MINT-364784.
STRINGi 4932.YOR212W.

Proteomic databases

MaxQBi P18851.
PaxDbi P18851.
PeptideAtlasi P18851.

Protocols and materials databases

DNASUi 854387.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR212W ; YOR212W ; YOR212W .
GeneIDi 854387.
KEGGi sce:YOR212W.

Organism-specific databases

CYGDi YOR212w.
SGDi S000005738. STE4.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00740000115438.
HOGENOMi HOG000176356.
KOi K04536.
OMAi ATSFTGH.
OrthoDBi EOG7JT75V.

Enzyme and pathway databases

BioCyci YEAST:G3O-33714-MONOMER.

Miscellaneous databases

NextBioi 976538.

Gene expression databases

Genevestigatori P18851.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 5 hits.
[Graphical view ]
PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
PRINTSi PR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 2 hits.
PROSITEi PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein."
    Whiteway M., Hougan L., Dignard D., Thomas D.Y., Bell L., Saari G.C., Grant F.J., O'Hara P., Mackay V.L.
    Cell 56:467-477(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
    Galisson F., Dujon B.
    Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit."
    Spain B.H., Koo D., Ramakrishnan M., Dzudzor B., Colicelli J.
    J. Biol. Chem. 270:25435-25444(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SYG1.
  6. "Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast."
    Flanary P.L., DiBello P.R., Estrada P., Dohlman H.G.
    J. Biol. Chem. 275:18462-18469(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLP1 AND PLP2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Gaitatzes C.G., Neer E.J., Smith T.F.
    Submitted (FEB-1998) to the PDB data bank
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiGBB_YEAST
AccessioniPrimary (citable) accession number: P18851
Secondary accession number(s): D6W2R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2050 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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