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Protein

Guanine nucleotide-binding protein subunit beta

Gene

STE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Implicated in the a- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Miscellaneous

Present with 2050 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • signal transducer activity, downstream of receptor Source: SGD

GO - Biological processi

  • chemotropism Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  • regulation of transposition, RNA-mediated Source: SGD

Keywordsi

Molecular functionTransducer
Biological processPheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-33714-MONOMER
ReactomeiR-SCE-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta
Gene namesi
Name:STE4
Ordered Locus Names:YOR212W
ORF Names:YOR50-2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR212W
SGDiS000005738 STE4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001277201 – 423Guanine nucleotide-binding protein subunit betaAdd BLAST423

Proteomic databases

MaxQBiP18851
PaxDbiP18851
PRIDEiP18851

PTM databases

iPTMnetiP18851

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. The beta-gamma subunit complex (STE4-STE18 complex) interacts with PLP1 and PLP2. Interacts with SYG1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34607, 85 interactors
DIPiDIP-954N
IntActiP18851, 65 interactors
MINTiP18851
STRINGi4932.YOR212W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SCGmodel-B1-423[»]
ProteinModelPortaliP18851
SMRiP18851
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati90 – 120WD 1Add BLAST31
Repeati132 – 162WD 2Add BLAST31
Repeati179 – 208WD 3Add BLAST30
Repeati220 – 256WD 4Add BLAST37
Repeati268 – 298WD 5Add BLAST31
Repeati348 – 377WD 6Add BLAST30
Repeati389 – 419WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00760000119239
HOGENOMiHOG000176356
InParanoidiP18851
KOiK04536
OMAiTCNVWDT
OrthoDBiEOG092C490G

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR016346 Guanine_nucleotide-bd_bsu
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19850 PTHR19850, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 4 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 2 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P18851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH
60 70 80 90 100
AQINKAKHKI QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW
110 120 130 140 150
SRDSKRILSA SQDGFMLIWD SASGLKQNAI PLDSQWVLSC AISPSSTLVA
160 170 180 190 200
SAGLNNNCTI YRVSKENRVA QNVASIFKGH TCYISDIEFT DNAHILTASG
210 220 230 240 250
DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS NTFASCGSDG
260 270 280 290 300
YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR
310 320 330 340 350
SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ
360 370 380 390 400
GVVSLDFSAS GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS
410 420
PDGLAVCTGS WDSTMKIWSP GYQ
Length:423
Mass (Da):46,581
Last modified:February 1, 1996 - v2
Checksum:i4B0E26040AAA83E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33D → E in AAA35114 (PubMed:2536595).Curated1
Sequence conflicti236S → L in AAA35114 (PubMed:2536595).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23982 Genomic DNA Translation: AAA35114.1
X92441 Genomic DNA Translation: CAA63175.1
Z75120 Genomic DNA Translation: CAA99427.1
BK006948 Genomic DNA Translation: DAA10984.1
PIRiS60939
RefSeqiNP_014855.3, NM_001183631.3

Genome annotation databases

EnsemblFungiiYOR212W; YOR212W; YOR212W
GeneIDi854387
KEGGisce:YOR212W

Similar proteinsi

Entry informationi

Entry nameiGBB_YEAST
AccessioniPrimary (citable) accession number: P18851
Secondary accession number(s): D6W2R8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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