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Reviewed, UniProtKB/Swiss-Prot P18848 (ATF4_HUMAN)

Last modified November 3, 2009. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cyclic AMP-dependent transcription factor ATF-4
      Short name=cAMP-dependent transcription factor ATF-4
Alternative name(s):
    Activating transcription factor 4
    DNA-binding protein TAXREB67
    Cyclic AMP-responsive element-binding protein 2
      Short name=cAMP-responsive element-binding protein 2
      Short name=CREB-2
Gene names
Name: ATF4
Synonyms: CREB2, TXREB
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Ref.7

Subunit structure

Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors By similarity. Interacts with the C-terminal region of GABBR1 via the leucine zipper of its C-terminal bZIP domain. Interacts with the C-terminal region of GABBR2 By similarity. Binds DNA as a homo- or heterodimer. Interacts with the N-terminal region of CEP290.

Subcellular location

Cytoplasm. Cell membrane. Nucleus. Note: Colocalizes with GABBR1 in hippocampal neuron dendritic membranes By similarity.

Sequence similarities

Belongs to the bZIP family.

Contains 1 bZIP domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CebpbP280331EBI-492498,EBI-1029979From a different organism.
DISC1Q9NRI53EBI-492498,EBI-529989
TRIB3Q96RU71EBI-492498,EBI-492476

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Cyclic AMP-dependent transcription factor ATF-4
PRO_0000076584

Regions

Domain306 – 33429Leucine-zipper
DNA binding280 – 30021Basic motif
Region305 – 34137Interaction with GABBR1 By similarity
Coiled coil280 – 34061

Natural variations

Natural variant221Q → P: dbSNP rs4894. Ref.1 Ref.2 Ref.4
VAR_028253
Natural variant2581P → A: dbSNP rs1803323.
VAR_029259
Natural variant3221E → D: dbSNP rs1803324.
VAR_014768

Experimental info

Sequence conflict2841K → R Ref.2
Sequence conflict2901T → R Ref.5
Sequence conflict329 – 3313KEI → REK Ref.5
Sequence conflict3381I → L Ref.5

Secondary structure

... 351
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18848-1 [UniParc].

Last modified October 17, 2006. Version 3.
Checksum: 3BBB7379DC3B0D07

FASTA35138,590
        10         20         30         40         50         60 
MTEMSFLSSE VLVGDLMSPF DQSGLGAEES LGLLDDYLEV AKHFKPHGFS SDKAKAGSSE 

        70         80         90        100        110        120 
WLAVDGLVSP SNNSKEDAFS GTDWMLEKMD LKEFDLDALL GIDDLETMPD DLLTTLDDTC 

       130        140        150        160        170        180 
DLFAPLVQET NKQPPQTVNP IGHLPESLTK PDQVAPFTFL QPLPLSPGVL SSTPDHSFSL 

       190        200        210        220        230        240 
ELGSEVDITE GDRKPDYTAY VAMIPQCIKE EDTPSDNDSG ICMSPESYLG SPQHSPSTRG 

       250        260        270        280        290        300 
SPNRSLPSPG VLCGSARPKP YDPPGEKMVA AKVKGEKLDK KLKKMEQNKT AATRYRQKKR 

       310        320        330        340        350 
AEQEALTGEC KELEKKNEAL KERADSLAKE IQYLKDLIEE VRKARGKKRV P 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I."
Tsujimoto A., Nyunoya H., Morita T., Sato T., Shimotohno K.
J. Virol. 65:1420-1426(1991) [PubMed: 1847461] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-22.
Tissue: Fibroblast.
[2]"Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element."
Karpinski B.A., Morle G.D., Huggenvik J., Uhler M.D., Leiden J.M.
Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992) [PubMed: 1534408] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-22.
Tissue: Leukemic T-cell.
[3]"The DNA sequence of human chromosome 22."
Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. expand/collapse author list , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
Nature 402:489-495(1999) [PubMed: 10591208] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-22.
Tissue: Lung and Placenta.
[5]"Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers."
Hai T., Liu F., Coukos W.J., Green M.R.
Genes Dev. 3:2083-2090(1989) [PubMed: 2516827] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 274-341.
[6]Erratum
Hai T., Liu F., Coukos W.J., Green M.R.
Genes Dev. 4:682-682(1990)
[7]"The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4."
Sayer J.A., Otto E.A., O'toole J.F., Nurnberg G., Kennedy M.A., Becker C., Hennies H.C., Helou J., Attanasio M., Fausett B.V., Utsch B., Khanna H., Liu Y., Drummond I., Kawakami I., Kusakabe T., Tsuda M., Ma L. expand/collapse author list , Lee H., Larson R.G., Allen S.J., Wilkinson C.J., Nigg E.A., Shou C., Lillo C., Williams D.S., Hoppe B., Kemper M.J., Neuhaus T., Parisi M.A., Glass I.A., Petry M., Kispert A., Gloy J., Ganner A., Walz G., Zhu X., Goldman D., Nurnberg P., Swaroop A., Leroux M.R., Hildebrandt F.
Nat. Genet. 38:674-681(2006) [PubMed: 16682973] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CEP290.
[8]"Crystal structure of the CCAAT box/enhancer-binding protein beta activating transcription factor-4 basic leucine zipper heterodimer in the absence of DNA."
Podust L.M., Krezel A.M., Kim Y.
J. Biol. Chem. 276:505-513(2001) [PubMed: 11018027] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 279-341 IN COMPLEX WITH MOUSE CEBPB.
+Additional computationally mapped references.

Cross-references

Sequence databases

D90209 mRNA. Translation: BAA14234.1.
M86842 mRNA. Translation: AAA52071.1.
AL022312 Genomic DNA. Translation: CAB45284.1.
BC008090 mRNA. Translation: AAH08090.1.
BC011994 mRNA. Translation: AAH11994.1.
BC024775 mRNA. Translation: AAH24775.1.
BC073990 mRNA. Translation: AAH73990.1.
IPIIPI00002503.
PIRA45377.
RefSeqNP_001666.2.
NP_877962.1.
UniGeneHs.496487

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1CI6X-ray2.60A280-341[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:207N.
DIP:354N.
IntActP18848. 7 interactions.
STRINGP18848.

PTM databases

PhosphoSiteP18848.

Proteomic databases

PRIDEP18848.

Genome annotation databases

EnsemblENST00000337304; ENSP00000336790; ENSG00000128272; Homo sapiens. [Genome view]
ENST00000396680; ENSP00000379912; ENSG00000128272; Homo sapiens. [Genome view]
ENST00000404241; ENSP00000384587; ENSG00000128272; Homo sapiens. [Genome view]
GeneID468.
KEGGhsa:468.
UCSCuc003axz.1. human.

Organism-specific databases

CTD468.
GeneCardsGC17M071736.
GC22P038241.
HGNCHGNC:786. ATF4.
HPACAB011596.
MIM604064. gene.
PharmGKBPA25086.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP18848.
HOVERGENP18848.
OMASYLGSPQ.

Enzyme and pathway databases

ReactomeREACT_15380. Diabetes pathways.

Gene expression databases

BgeeP18848.
CleanExHS_ATF4.
GenevestigatorP18848.
GermOnlineENSG00000128272. Homo sapiens.

Family and domain databases

InterProIPR011616. bZIP_1.
IPR004827. TF_bZIP.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio1935.
SOURCESearch...

Entry information

Entry nameATF4_HUMAN
AccessionPrimary (citable) accession number: P18848
Secondary accession number(s): Q9UH31
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 17, 2006
Last modified: November 3, 2009
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents