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Reviewed, UniProtKB/Swiss-Prot P18847 (ATF3_HUMAN)

Last modified January 19, 2010. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cyclic AMP-dependent transcription factor ATF-3
      Short name=cAMP-dependent transcription factor ATF-3
Alternative name(s):
    Activating transcription factor 3
Gene names
Name: ATF3
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Subunit structure

Binds DNA as a homodimer or a heterodimer.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.

Contains 1 bZIP domain.

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Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandDNA-binding
   Molecular functionRepressor
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtranscription

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentnucleolus

Inferred from direct assay. Source: HPA

   Molecular functionidentical protein binding

Inferred from physical interaction. Source: IntAct

protein dimerization activity

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

transcription corepressor activity Ref.1

Traceable author statement. Source: ProtInc

transcription factor activity Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-712767,EBI-712767
FHL2Q141921EBI-712767,EBI-701903
RELAQ042061EBI-712767,EBI-73886

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P18847-1)

Also known as: ATF3;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P18847-2)

Also known as: ATF3-delta-Zip;

The sequence of this isoform differs from the canonical sequence as follows:
     116-181: KESEKLESVNAELKAQIEELKNEKQHLIYMLNLHRPTCIVRAQNGRTPEDERNLFIQQIKEGTLQS → LQY
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181Cyclic AMP-dependent transcription factor ATF-3
PRO_0000076581

Regions

Domain114 – 14229Leucine-zipper
DNA binding88 – 11023Basic motif

Natural variations

Alternative sequence116 – 18166KESEK…GTLQS → LQY in isoform 2.
VSP_000592
Natural variant381T → M: dbSNP rs11571541. Ref.2
VAR_048442

Experimental info

Sequence conflict1321I → L Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ATF3) [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: EC5D8F065EEE2D9C

FASTA18120,576
        10         20         30         40         50         60 
MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS 

        70         80         90        100        110        120 
ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK 

       130        140        150        160        170        180 
LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ 


S

« Hide

Isoform 2 (ATF3-delta-Zip).

Checksum: E0444ABC236FA838
Show »

FASTA11813,266

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References

« Hide 'large scale' references
[1]"ATF3 and ATF3 delta Zip. Transcriptional repression versus activation by alternatively spliced isoforms."
Chen B.P.C., Liang G., Whelan J., Hai T.
J. Biol. Chem. 269:15819-15826(1994) [PubMed: 7515060] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]NIEHS SNPs program
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT MET-38.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Muscle.
[4]"Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers."
Hai T., Liu F., Coukos W.J., Green M.R.
Genes Dev. 3:2083-2090(1989) [PubMed: 2516827] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-181.
[5]Erratum
Hai T., Liu F., Coukos W.J., Green M.R.
Genes Dev. 4:682-682(1990)
[6]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
+Additional computationally mapped references.

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Web resources

NIEHS-SNPs

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L19871 mRNA. Translation: AAA20506.1.
AY426987 Genomic DNA. Translation: AAQ93358.1.
BC006322 mRNA. Translation: AAH06322.1.
IPIIPI00002502.
IPI00413177.
PIRA54025.
B54025.
C34223.
RefSeqNP_001025458.1.
NP_001665.1.
UniGeneHs.460

3D structure databases

SMRP18847. Positions 90-140.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-254N.
DIP-344N.
IntActP18847. 10 interactions.
STRINGP18847.

Proteomic databases

PRIDEP18847.

Genome annotation databases

EnsemblENST00000341491; ENSP00000344352; ENSG00000162772; Homo sapiens. [Genome view]
ENST00000366987; ENSP00000355954; ENSG00000162772; Homo sapiens. [Genome view]
GeneID467.
KEGGhsa:467.
UCSCuc001hjf.1. human.

Organism-specific databases

CTD467.
GeneCardsGC01P210805.
H-InvDBHIX0001571.
HGNCHGNC:785. ATF3.
HPAHPA001562.
MIM603148. gene.
PharmGKBPA25085.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG11208.
HOGENOMHBG444691.
HOVERGENP18847.
InParanoidP18847.
OMASDRPLEM.
PhylomeDBP18847.

Enzyme and pathway databases

Pathway_Interaction_DBsmad2_3nuclearpathway. Regulation of nuclear SMAD2/3 signaling.
ReactomeREACT_15380. Diabetes pathways.

Gene expression databases

ArrayExpressP18847.
BgeeP18847.
CleanExHS_ATF3.
GenevestigatorP18847.
GermOnlineENSG00000162772. Homo sapiens.

Family and domain databases

InterProIPR011616. bZIP_1.
IPR008917. Euk_TF_DNA_bd.
IPR000837. Leuzip_Fos.
IPR004827. TF_bZIP.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
[Graphical view]
PRINTSPR00042. LEUZIPPRFOS.
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio1925.
SOURCESearch...

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Entry information

Entry nameATF3_HUMAN
AccessionPrimary (citable) accession number: P18847
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1995
Last modified: January 19, 2010
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents