P18847 (ATF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclic AMP-dependent transcription factor ATF-3
Short name=cAMP-dependent transcription factor ATF-3

Alternative name(s):
Activating transcription factor 3

Gene names

Name:

ATF3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Subunit structure	Binds DNA as a homodimer or a heterodimer.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from direct assay. Source: HPA nucleoplasm Traceable author statement. Source: Reactome
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Traceable author statement. Source: ProtInc transcription corepressor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-712767,EBI-712767
FHL2	Q14192	3	EBI-712767,EBI-701903
RELA	Q04206	4	EBI-712767,EBI-73886

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P18847-1)

Also known as: ATF3;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P18847-2)

Also known as: ATF3-delta-Zip;

The sequence of this isoform differs from the canonical sequence as follows:
     116-181: KESEKLESVNAELKAQIEELKNEKQHLIYMLNLHRPTCIVRAQNGRTPEDERNLFIQQIKEGTLQS → LQY

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Cyclic AMP-dependent transcription factor ATF-3		PRO_0000076581
Regions
	Domain	114 – 142	29	Leucine-zipper
	DNA binding	88 – 110	23	Basic motif
Natural variations
	Alternative sequence	116 – 181	66	KESEK…GTLQS → LQY in isoform 2.		VSP_000592
	Natural variant	38	1	T → M. Ref.2 Corresponds to variant rs11571541 [ dbSNP | Ensembl ].		VAR_048442
Experimental info
	Sequence conflict	132	1	I → L no nucleotide entry Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ATF3) [UniParc]. Last modified November 1, 1995. Version 2. Checksum: EC5D8F065EEE2D9C	FASTA	181	20,576
	10 20 30 40 50 60 MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS 70 80 90 100 110 120 ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK 130 140 150 160 170 180 LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S
	Isoform 2 (ATF3-delta-Zip) [UniParc]. Checksum: E0444ABC236FA838	FASTA	118	13,266
	10 20 30 40 50 60 MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS 70 80 90 100 110 ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQLQY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ATF3 and ATF3 delta Zip. Transcriptional repression versus activation by alternatively spliced isoforms." Chen B.P.C., Liang G., Whelan J., Hai T. J. Biol. Chem. 269:15819-15826(1994) [PubMed: 7515060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	NIEHS SNPs program Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT MET-38.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
[4]	"Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers." Hai T., Liu F., Coukos W.J., Green M.R. Genes Dev. 3:2083-2090(1989) [PubMed: 2516827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-181.
[5]	Erratum Hai T., Liu F., Coukos W.J., Green M.R. Genes Dev. 4:682-682(1990)
[6]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19871 mRNA. Translation: AAA20506.1. AY426987 Genomic DNA. Translation: AAQ93358.1. BC006322 mRNA. Translation: AAH06322.1.
IPI	IPI00002502. IPI01009242.
PIR	A54025. B54025. C34223.
RefSeq	NP_001025458.1. NM_001030287.3. NP_001035709.1. NM_001040619.2. NP_001193413.2. NM_001206484.2. NP_001193415.1. NM_001206486.2. NP_001193417.2. NM_001206488.2. NP_001665.1. NM_001674.3.
UniGene	Hs.460.
3D structure databases
ProteinModelPortal	P18847.
SMR	P18847. Positions 87-149.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-254N. DIP-344N.
IntAct	P18847. 11 interactions.
MINT	MINT-4649546.
STRING	P18847.
Polymorphism databases
DMDM	1168543.
Proteomic databases
PRIDE	P18847.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000341491; ENSP00000344352; ENSG00000162772. ENST00000366987; ENSP00000355954; ENSG00000162772.
GeneID	467.
KEGG	hsa:467.
UCSC	uc001hjf.1. human.
Organism-specific databases
CTD	467.
GeneCards	GC01P212738.
H-InvDB	HIX0001571.
HGNC	HGNC:785. ATF3.
HPA	HPA001562.
MIM	603148. gene.
neXtProt	NX_P18847.
PharmGKB	PA25085.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11208.
HOGENOM	HBG444691.
HOVERGEN	HBG003381.
InParanoid	P18847.
OMA	ATAIVPC.
OrthoDB	EOG41NTN9.
PhylomeDB	P18847.
Enzyme and pathway databases
Pathway_Interaction_DB	smad2_3nuclearpathway. Regulation of nuclear SMAD2/3 signaling.
Reactome	REACT_15380. Diabetes pathways.
Gene expression databases
ArrayExpress	P18847.
Bgee	P18847.
CleanEx	HS_ATF3.
Genevestigator	P18847.
GermOnline	ENSG00000162772. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. IPR011616. bZIP_1. IPR000837. Leuzip_Fos. [Graphical view]
KO	K09032.
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
PRINTS	PR00042. LEUZIPPRFOS.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	1925.
SOURCE	Search...

Entry information

Entry name

ATF3_HUMAN

Accession

Primary (citable) accession number: P18847

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.