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Protein

Cyclic AMP-dependent transcription factor ATF-1

Gene

ATF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000123268-MONOMER.
ReactomeiR-HSA-199920. CREB phosphorylation.
SignaLinkiP18846.
SIGNORiP18846.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-dependent transcription factor ATF-1
Short name:
cAMP-dependent transcription factor ATF-1
Alternative name(s):
Activating transcription factor 1
Protein TREB36
Gene namesi
Name:ATF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:783. ATF1.

Subcellular locationi

GO - Cellular componenti

  • ATF1-ATF4 transcription factor complex Source: ParkinsonsUK-UCL
  • nucleoplasm Source: HPA
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Angiomatoid fibrous histiocytoma (AFH)
The gene represented in this entry may be involved in disease pathogenesis. Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
Disease descriptionA distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis.
See also OMIM:612160

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63S → A: Impaired CDK3-mediated phosphorylation and altered transactivation and transcriptional activities. 1 Publication1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110Breakpoint for translocation to form chimeric EWSR1/ATF1 protein1
Sitei112Breakpoint for translocation to form chimeric FUS/ATF1 protein1

Organism-specific databases

DisGeNETi466.
MalaCardsiATF1.
MIMi612160. phenotype.
OpenTargetsiENSG00000123268.
Orphaneti97338. Melanoma of soft parts.
PharmGKBiPA25083.

Chemistry databases

ChEMBLiCHEMBL3255.
DrugBankiDB00852. Pseudoephedrine.

Polymorphism and mutation databases

BioMutaiATF1.
DMDMi1168542.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765751 – 271Cyclic AMP-dependent transcription factor ATF-1Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63Phosphoserine; by CaMK1, CDK3, RPS6KA4 and RPS6KA52 Publications1
Modified residuei198Phosphoserine; by HIPK2PROSITE-ProRule annotationCombined sources1 Publication1

Post-translational modificationi

Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP18846.
MaxQBiP18846.
PaxDbiP18846.
PeptideAtlasiP18846.
PRIDEiP18846.

PTM databases

iPTMnetiP18846.
PhosphoSitePlusiP18846.

Expressioni

Gene expression databases

BgeeiENSG00000123268.
CleanExiHS_ATF1.
ExpressionAtlasiP18846. baseline and differential.
GenevisibleiP18846. HS.

Organism-specific databases

HPAiCAB016222.
HPA055069.
HPA055406.

Interactioni

Subunit structurei

Binds DNA as a dimer. Interacts with HIPK2 and CDK3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P03259-22EBI-852794,EBI-7225021From a different organism.
CREB1P162206EBI-852794,EBI-711855
HBZP0C7462EBI-852794,EBI-10890294From a different organism.
JUNP054122EBI-852794,EBI-852823
MDV005Q9DGW52EBI-852794,EBI-10889526From a different organism.
NFATC1O956443EBI-852794,EBI-6907210
NFIL3Q166492EBI-852794,EBI-3951858

Protein-protein interaction databases

BioGridi106956. 28 interactors.
DIPiDIP-652N.
IntActiP18846. 11 interactors.
MINTiMINT-142718.
STRINGi9606.ENSP00000262053.

Chemistry databases

BindingDBiP18846.

Structurei

3D structure databases

ProteinModelPortaliP18846.
SMRiP18846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 90KIDPROSITE-ProRule annotationAdd BLAST60
Domaini213 – 271bZIPPROSITE-ProRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 239Basic motifPROSITE-ProRule annotationAdd BLAST25
Regioni241 – 262Leucine-zipperPROSITE-ProRule annotationAdd BLAST22

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation
Contains 1 KID (kinase-inducible) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3584. Eukaryota.
ENOG410ZZJZ. LUCA.
GeneTreeiENSGT00390000008655.
HOGENOMiHOG000007365.
HOVERGENiHBG011077.
InParanoidiP18846.
KOiK09053.
OMAiHIAQQMS.
OrthoDBiEOG091G0FTJ.
PhylomeDBiP18846.
TreeFamiTF106464.

Family and domain databases

InterProiIPR029825. ATF1.
IPR004827. bZIP.
IPR003102. Coactivator_CBP_pKID.
IPR001630. Leuzip_CREB.
[Graphical view]
PANTHERiPTHR22952:SF148. PTHR22952:SF148. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
PF02173. pKID. 1 hit.
[Graphical view]
PRINTSiPR00041. LEUZIPPRCREB.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
PS50953. KID. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P18846-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDSHKSTTS ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS
60 70 80 90 100
SQKAHGILAR RPSYRKILKD LSSEDTRGRK GDGENSGVSA AVTSMSVPTP
110 120 130 140 150
IYQTSSGQYI AIAPNGALQL ASPGTDGVQG LQTLTMTNSG STQQGTTILQ
160 170 180 190 200
YAQTSDGQQI LVPSNQVVVQ TASGDMQTYQ IRTTPSATSL PQTVVMTSPV
210 220 230 240 250
TLTSQTTKTD DPQLKREIRL MKNREAAREC RRKKKEYVKC LENRVAVLEN
260 270
QNKTLIEELK TLKDLYSNKS V
Length:271
Mass (Da):29,233
Last modified:November 1, 1995 - v2
Checksum:iBAFECBFFB244FCED
GO
Isoform 2 (identifier: P18846-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: Missing.

Note: No experimental confirmation available.
Show »
Length:136
Mass (Da):15,295
Checksum:i86343F1CF592889F
GO

Sequence cautioni

The sequence CAC15058 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55H → A no nucleotide entry (PubMed:2516827).Curated1
Sequence conflicti227Missing no nucleotide entry (PubMed:2516827).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024382191P → A.Corresponds to variant rs2230674dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0555591 – 135Missing in isoform 2. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55544 Genomic DNA. Translation: CAA39150.1.
AK299240 mRNA. Translation: BAG61271.1.
AC013244 Genomic DNA. No translation available.
BC029619 mRNA. Translation: AAH29619.1.
AJ295163 mRNA. Translation: CAC15058.1. Different initiation.
CCDSiCCDS8803.1. [P18846-1]
PIRiS12560.
RefSeqiNP_005162.1. NM_005171.4. [P18846-1]
XP_011536688.1. XM_011538386.2. [P18846-1]
XP_011536689.1. XM_011538387.2. [P18846-1]
XP_016874821.1. XM_017019332.1. [P18846-1]
XP_016874822.1. XM_017019333.1. [P18846-1]
XP_016874823.1. XM_017019334.1. [P18846-1]
UniGeneiHs.648565.

Genome annotation databases

EnsembliENST00000262053; ENSP00000262053; ENSG00000123268. [P18846-1]
GeneIDi466.
KEGGihsa:466.
UCSCiuc001rww.5. human. [P18846-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55544 Genomic DNA. Translation: CAA39150.1.
AK299240 mRNA. Translation: BAG61271.1.
AC013244 Genomic DNA. No translation available.
BC029619 mRNA. Translation: AAH29619.1.
AJ295163 mRNA. Translation: CAC15058.1. Different initiation.
CCDSiCCDS8803.1. [P18846-1]
PIRiS12560.
RefSeqiNP_005162.1. NM_005171.4. [P18846-1]
XP_011536688.1. XM_011538386.2. [P18846-1]
XP_011536689.1. XM_011538387.2. [P18846-1]
XP_016874821.1. XM_017019332.1. [P18846-1]
XP_016874822.1. XM_017019333.1. [P18846-1]
XP_016874823.1. XM_017019334.1. [P18846-1]
UniGeneiHs.648565.

3D structure databases

ProteinModelPortaliP18846.
SMRiP18846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106956. 28 interactors.
DIPiDIP-652N.
IntActiP18846. 11 interactors.
MINTiMINT-142718.
STRINGi9606.ENSP00000262053.

Chemistry databases

BindingDBiP18846.
ChEMBLiCHEMBL3255.
DrugBankiDB00852. Pseudoephedrine.

PTM databases

iPTMnetiP18846.
PhosphoSitePlusiP18846.

Polymorphism and mutation databases

BioMutaiATF1.
DMDMi1168542.

Proteomic databases

EPDiP18846.
MaxQBiP18846.
PaxDbiP18846.
PeptideAtlasiP18846.
PRIDEiP18846.

Protocols and materials databases

DNASUi466.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262053; ENSP00000262053; ENSG00000123268. [P18846-1]
GeneIDi466.
KEGGihsa:466.
UCSCiuc001rww.5. human. [P18846-1]

Organism-specific databases

CTDi466.
DisGeNETi466.
GeneCardsiATF1.
HGNCiHGNC:783. ATF1.
HPAiCAB016222.
HPA055069.
HPA055406.
MalaCardsiATF1.
MIMi123803. gene.
612160. phenotype.
neXtProtiNX_P18846.
OpenTargetsiENSG00000123268.
Orphaneti97338. Melanoma of soft parts.
PharmGKBiPA25083.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3584. Eukaryota.
ENOG410ZZJZ. LUCA.
GeneTreeiENSGT00390000008655.
HOGENOMiHOG000007365.
HOVERGENiHBG011077.
InParanoidiP18846.
KOiK09053.
OMAiHIAQQMS.
OrthoDBiEOG091G0FTJ.
PhylomeDBiP18846.
TreeFamiTF106464.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000123268-MONOMER.
ReactomeiR-HSA-199920. CREB phosphorylation.
SignaLinkiP18846.
SIGNORiP18846.

Miscellaneous databases

ChiTaRSiATF1. human.
GeneWikiiATF1.
GenomeRNAii466.
PROiP18846.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123268.
CleanExiHS_ATF1.
ExpressionAtlasiP18846. baseline and differential.
GenevisibleiP18846. HS.

Family and domain databases

InterProiIPR029825. ATF1.
IPR004827. bZIP.
IPR003102. Coactivator_CBP_pKID.
IPR001630. Leuzip_CREB.
[Graphical view]
PANTHERiPTHR22952:SF148. PTHR22952:SF148. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
PF02173. pKID. 1 hit.
[Graphical view]
PRINTSiPR00041. LEUZIPPRCREB.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
PS50953. KID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATF1_HUMAN
AccessioniPrimary (citable) accession number: P18846
Secondary accession number(s): B4DRF9, P25168, Q9H4A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.