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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction.

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NAD1 Publication1
Binding sitei82ATP1 Publication1
Binding sitei88ATPBy similarity1
Binding sitei140NAD1 Publication1
Binding sitei160ATP1 Publication1
Binding sitei211ATPBy similarity1
Binding sitei261NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATP1 Publication8
Nucleotide bindingi170 – 180NAD1 PublicationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

  • 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • NAD salvage Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NAD-SYNTH-MONOMER.
ECOL316407:JW1729-MONOMER.
MetaCyc:NAD-SYNTH-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetase (EC:6.3.1.5)
Alternative name(s):
Nicotinamide adenine dinucleotide synthetase
Short name:
NADS
Nitrogen regulatory protein
Gene namesi
Name:nadE
Synonyms:efg, ntrL
Ordered Locus Names:b1740, JW1729
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10663. nadE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521671 – 275NH(3)-dependent NAD(+) synthetaseAdd BLAST275

Post-translational modificationi

May be phosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP18843.
PaxDbiP18843.
PRIDEiP18843.

2D gel databases

SWISS-2DPAGEP18843.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4262229. 9 interactors.
DIPiDIP-10295N.
IntActiP18843. 480 interactors.
MINTiMINT-1232772.
STRINGi511145.b1740.

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Helixi19 – 36Combined sources18
Beta strandi42 – 46Combined sources5
Helixi51 – 71Combined sources21
Beta strandi77 – 82Combined sources6
Beta strandi85 – 87Combined sources3
Helixi91 – 101Combined sources11
Beta strandi104 – 108Combined sources5
Helixi112 – 125Combined sources14
Helixi131 – 152Combined sources22
Beta strandi155 – 158Combined sources4
Helixi163 – 166Combined sources4
Turni167 – 169Combined sources3
Turni173 – 177Combined sources5
Turni183 – 186Combined sources4
Helixi189 – 198Combined sources10
Helixi203 – 205Combined sources3
Helixi224 – 227Combined sources4
Helixi231 – 238Combined sources8
Helixi245 – 257Combined sources13
Helixi259 – 262Combined sources4
Helixi272 – 274Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXEX-ray1.90A1-275[»]
1WXFX-ray2.30A1-275[»]
1WXGX-ray1.90A1-275[»]
1WXHX-ray1.90A1-275[»]
1WXIX-ray1.70A1-275[»]
ProteinModelPortaliP18843.
SMRiP18843.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18843.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
InParanoidiP18843.
KOiK01916.
OMAiAKIIEGW.
PhylomeDBiP18843.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

P18843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQQQIIKA LGAKPQINAE EEIRRSVDFL KSYLQTYPFI KSLVLGISGG
60 70 80 90 100
QDSTLAGKLC QMAINELRLE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF
110 120 130 140 150
IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA
160 170 180 190 200
GMTSGVVVGT DHAAEAITGF FTKYGDGGTD INPLYRLNKR QGKQLLAALA
210 220 230 240 250
CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG KNVPQQVART
260 270
IENWYLKTEH KRRPPITVFD DFWKK
Length:275
Mass (Da):30,637
Last modified:November 1, 1997 - v2
Checksum:i85EE6EE01C648282
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13 – 31AKPQI…VDFLK → ENRRLMLKRKFVVVSISE in AAA79852 (PubMed:3025172).CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15328 Genomic DNA. Translation: AAA79852.1.
U00096 Genomic DNA. Translation: AAC74810.1.
AP009048 Genomic DNA. Translation: BAA15529.1.
PIRiD64933.
RefSeqiNP_416254.1. NC_000913.3.
WP_000175026.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74810; AAC74810; b1740.
BAA15529; BAA15529; BAA15529.
GeneIDi946946.
KEGGiecj:JW1729.
eco:b1740.
PATRICi32118789. VBIEscCol129921_1812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15328 Genomic DNA. Translation: AAA79852.1.
U00096 Genomic DNA. Translation: AAC74810.1.
AP009048 Genomic DNA. Translation: BAA15529.1.
PIRiD64933.
RefSeqiNP_416254.1. NC_000913.3.
WP_000175026.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXEX-ray1.90A1-275[»]
1WXFX-ray2.30A1-275[»]
1WXGX-ray1.90A1-275[»]
1WXHX-ray1.90A1-275[»]
1WXIX-ray1.70A1-275[»]
ProteinModelPortaliP18843.
SMRiP18843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262229. 9 interactors.
DIPiDIP-10295N.
IntActiP18843. 480 interactors.
MINTiMINT-1232772.
STRINGi511145.b1740.

2D gel databases

SWISS-2DPAGEP18843.

Proteomic databases

EPDiP18843.
PaxDbiP18843.
PRIDEiP18843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74810; AAC74810; b1740.
BAA15529; BAA15529; BAA15529.
GeneIDi946946.
KEGGiecj:JW1729.
eco:b1740.
PATRICi32118789. VBIEscCol129921_1812.

Organism-specific databases

EchoBASEiEB0657.
EcoGeneiEG10663. nadE.

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
InParanoidiP18843.
KOiK01916.
OMAiAKIIEGW.
PhylomeDBiP18843.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciEcoCyc:NAD-SYNTH-MONOMER.
ECOL316407:JW1729-MONOMER.
MetaCyc:NAD-SYNTH-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP18843.
PROiP18843.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_ECOLI
AccessioniPrimary (citable) accession number: P18843
Secondary accession number(s): P78235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.