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P18843

- NADE_ECOLI

UniProt

P18843 - NADE_ECOLI

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Protein
NH(3)-dependent NAD(+) synthetase
Gene
nadE, efg, ntrL, b1740, JW1729
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331NAD
Binding sitei82 – 821ATP
Binding sitei88 – 881ATP By similarity
Binding sitei140 – 1401NAD
Binding sitei160 – 1601ATP
Binding sitei211 – 2111ATP By similarity
Binding sitei261 – 2611NAD

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538ATPUniRule annotation
Nucleotide bindingi170 – 18011NADUniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. NAD+ synthase (glutamine-hydrolyzing) activity Source: InterPro
  3. NAD+ synthase activity Source: EcoCyc

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
  2. NAD salvage Source: EcoCyc
  3. cellular response to DNA damage stimulus Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NAD-SYNTH-MONOMER.
ECOL316407:JW1729-MONOMER.
MetaCyc:NAD-SYNTH-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetase (EC:6.3.1.5)
Alternative name(s):
Nicotinamide adenine dinucleotide synthetase
Short name:
NADS
Nitrogen regulatory protein
Gene namesi
Name:nadE
Synonyms:efg, ntrL
Ordered Locus Names:b1740, JW1729
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10663. nadE.

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275NH(3)-dependent NAD(+) synthetaseUniRule annotation
PRO_0000152167Add
BLAST

Post-translational modificationi

May be phosphorylated.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP18843.
PRIDEiP18843.

2D gel databases

SWISS-2DPAGEP18843.

Expressioni

Gene expression databases

GenevestigatoriP18843.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-10295N.
IntActiP18843. 480 interactions.
MINTiMINT-1232772.
STRINGi511145.b1740.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 119
Helixi19 – 3618
Beta strandi42 – 465
Helixi51 – 7121
Beta strandi77 – 826
Beta strandi85 – 873
Helixi91 – 10111
Beta strandi104 – 1085
Helixi112 – 12514
Helixi131 – 15222
Beta strandi155 – 1584
Helixi163 – 1664
Turni167 – 1693
Turni173 – 1775
Turni183 – 1864
Helixi189 – 19810
Helixi203 – 2053
Helixi224 – 2274
Helixi231 – 2388
Helixi245 – 25713
Helixi259 – 2624
Helixi272 – 2743

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WXEX-ray1.90A1-275[»]
1WXFX-ray2.30A1-275[»]
1WXGX-ray1.90A1-275[»]
1WXHX-ray1.90A1-275[»]
1WXIX-ray1.70A1-275[»]
ProteinModelPortaliP18843.
SMRiP18843. Positions 2-275.

Miscellaneous databases

EvolutionaryTraceiP18843.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.

Phylogenomic databases

eggNOGiCOG0171.
HOGENOMiHOG000238070.
KOiK01916.
OMAiGQILREM.
OrthoDBiEOG64JFM7.
PhylomeDBiP18843.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

P18843-1 [UniParc]FASTAAdd to Basket

« Hide

MTLQQQIIKA LGAKPQINAE EEIRRSVDFL KSYLQTYPFI KSLVLGISGG    50
QDSTLAGKLC QMAINELRLE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF 100
IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA 150
GMTSGVVVGT DHAAEAITGF FTKYGDGGTD INPLYRLNKR QGKQLLAALA 200
CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG KNVPQQVART 250
IENWYLKTEH KRRPPITVFD DFWKK 275
Length:275
Mass (Da):30,637
Last modified:November 1, 1997 - v2
Checksum:i85EE6EE01C648282
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 3119AKPQI…VDFLK → ENRRLMLKRKFVVVSISE in AAA79852. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15328 Genomic DNA. Translation: AAA79852.1.
U00096 Genomic DNA. Translation: AAC74810.1.
AP009048 Genomic DNA. Translation: BAA15529.1.
PIRiD64933.
RefSeqiNP_416254.1. NC_000913.3.
YP_490001.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74810; AAC74810; b1740.
BAA15529; BAA15529; BAA15529.
GeneIDi12933235.
946946.
KEGGiecj:Y75_p1715.
eco:b1740.
PATRICi32118789. VBIEscCol129921_1812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15328 Genomic DNA. Translation: AAA79852.1 .
U00096 Genomic DNA. Translation: AAC74810.1 .
AP009048 Genomic DNA. Translation: BAA15529.1 .
PIRi D64933.
RefSeqi NP_416254.1. NC_000913.3.
YP_490001.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WXE X-ray 1.90 A 1-275 [» ]
1WXF X-ray 2.30 A 1-275 [» ]
1WXG X-ray 1.90 A 1-275 [» ]
1WXH X-ray 1.90 A 1-275 [» ]
1WXI X-ray 1.70 A 1-275 [» ]
ProteinModelPortali P18843.
SMRi P18843. Positions 2-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10295N.
IntActi P18843. 480 interactions.
MINTi MINT-1232772.
STRINGi 511145.b1740.

Chemistry

DrugBanki DB00131. Adenosine monophosphate.

2D gel databases

SWISS-2DPAGE P18843.

Proteomic databases

PaxDbi P18843.
PRIDEi P18843.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74810 ; AAC74810 ; b1740 .
BAA15529 ; BAA15529 ; BAA15529 .
GeneIDi 12933235.
946946.
KEGGi ecj:Y75_p1715.
eco:b1740.
PATRICi 32118789. VBIEscCol129921_1812.

Organism-specific databases

EchoBASEi EB0657.
EcoGenei EG10663. nadE.

Phylogenomic databases

eggNOGi COG0171.
HOGENOMi HOG000238070.
KOi K01916.
OMAi GQILREM.
OrthoDBi EOG64JFM7.
PhylomeDBi P18843.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00333 .
BioCyci EcoCyc:NAD-SYNTH-MONOMER.
ECOL316407:JW1729-MONOMER.
MetaCyc:NAD-SYNTH-MONOMER.

Miscellaneous databases

EvolutionaryTracei P18843.
PROi P18843.

Gene expression databases

Genevestigatori P18843.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00193. NadE.
InterProi IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF02540. NAD_synthase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00552. nadE. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complementation of nitrogen-regulatory (ntr-like) mutations in Rhodobacter capsulatus by an Escherichia coli gene: cloning and sequencing of the gene and characterization of the gene product."
    Allibert P., Willison J.C., Vignais P.M.
    J. Bacteriol. 169:260-271(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
    Strain: K12 / EMG2.
  6. "An essential gene (efg) located at 38.1 minutes on the Escherichia coli chromosome."
    Willison J.C.
    J. Bacteriol. 174:5765-5766(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE MAPPING.
  7. "The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase."
    Willison J.C., Tissot G.
    J. Bacteriol. 176:3400-3402(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements."
    Jauch R., Humm A., Huber R., Wahl M.C.
    J. Biol. Chem. 280:15131-15140(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF APOPROTEIN AND COMPLEX WITH SUBSTRATES; PRODUCTS AND COFACTOR.

Entry informationi

Entry nameiNADE_ECOLI
AccessioniPrimary (citable) accession number: P18843
Secondary accession number(s): P78235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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