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P18843

- NADE_ECOLI

UniProt

P18843 - NADE_ECOLI

Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction.

    Catalytic activityi

    ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331NAD
    Binding sitei82 – 821ATP
    Binding sitei88 – 881ATPBy similarity
    Binding sitei140 – 1401NAD
    Binding sitei160 – 1601ATP
    Binding sitei211 – 2111ATPBy similarity
    Binding sitei261 – 2611NAD

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 538ATP
    Nucleotide bindingi170 – 18011NADAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. NAD+ synthase (glutamine-hydrolyzing) activity Source: InterPro
    3. NAD+ synthase activity Source: EcoCyc

    GO - Biological processi

    1. 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
    2. cellular response to DNA damage stimulus Source: EcoliWiki
    3. NAD salvage Source: EcoCyc

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, NAD, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:NAD-SYNTH-MONOMER.
    ECOL316407:JW1729-MONOMER.
    MetaCyc:NAD-SYNTH-MONOMER.
    UniPathwayiUPA00253; UER00333.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NH(3)-dependent NAD(+) synthetase (EC:6.3.1.5)
    Alternative name(s):
    Nicotinamide adenine dinucleotide synthetase
    Short name:
    NADS
    Nitrogen regulatory protein
    Gene namesi
    Name:nadE
    Synonyms:efg, ntrL
    Ordered Locus Names:b1740, JW1729
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10663. nadE.

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275NH(3)-dependent NAD(+) synthetasePRO_0000152167Add
    BLAST

    Post-translational modificationi

    May be phosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP18843.
    PRIDEiP18843.

    2D gel databases

    SWISS-2DPAGEP18843.

    Expressioni

    Gene expression databases

    GenevestigatoriP18843.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-10295N.
    IntActiP18843. 480 interactions.
    MINTiMINT-1232772.
    STRINGi511145.b1740.

    Structurei

    Secondary structure

    1
    275
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 119
    Helixi19 – 3618
    Beta strandi42 – 465
    Helixi51 – 7121
    Beta strandi77 – 826
    Beta strandi85 – 873
    Helixi91 – 10111
    Beta strandi104 – 1085
    Helixi112 – 12514
    Helixi131 – 15222
    Beta strandi155 – 1584
    Helixi163 – 1664
    Turni167 – 1693
    Turni173 – 1775
    Turni183 – 1864
    Helixi189 – 19810
    Helixi203 – 2053
    Helixi224 – 2274
    Helixi231 – 2388
    Helixi245 – 25713
    Helixi259 – 2624
    Helixi272 – 2743

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WXEX-ray1.90A1-275[»]
    1WXFX-ray2.30A1-275[»]
    1WXGX-ray1.90A1-275[»]
    1WXHX-ray1.90A1-275[»]
    1WXIX-ray1.70A1-275[»]
    ProteinModelPortaliP18843.
    SMRiP18843. Positions 2-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18843.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0171.
    HOGENOMiHOG000238070.
    KOiK01916.
    OMAiGQILREM.
    OrthoDBiEOG64JFM7.
    PhylomeDBiP18843.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00193. NadE.
    InterProiIPR022310. NAD/GMP_synthase.
    IPR003694. NAD_synthase.
    IPR022926. NH(3)-dep_NAD(+)_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF02540. NAD_synthase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00552. nadE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18843-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLQQQIIKA LGAKPQINAE EEIRRSVDFL KSYLQTYPFI KSLVLGISGG    50
    QDSTLAGKLC QMAINELRLE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF 100
    IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA 150
    GMTSGVVVGT DHAAEAITGF FTKYGDGGTD INPLYRLNKR QGKQLLAALA 200
    CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG KNVPQQVART 250
    IENWYLKTEH KRRPPITVFD DFWKK 275
    Length:275
    Mass (Da):30,637
    Last modified:November 1, 1997 - v2
    Checksum:i85EE6EE01C648282
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 3119AKPQI…VDFLK → ENRRLMLKRKFVVVSISE in AAA79852. (PubMed:3025172)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15328 Genomic DNA. Translation: AAA79852.1.
    U00096 Genomic DNA. Translation: AAC74810.1.
    AP009048 Genomic DNA. Translation: BAA15529.1.
    PIRiD64933.
    RefSeqiNP_416254.1. NC_000913.3.
    YP_490001.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74810; AAC74810; b1740.
    BAA15529; BAA15529; BAA15529.
    GeneIDi12933235.
    946946.
    KEGGiecj:Y75_p1715.
    eco:b1740.
    PATRICi32118789. VBIEscCol129921_1812.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15328 Genomic DNA. Translation: AAA79852.1 .
    U00096 Genomic DNA. Translation: AAC74810.1 .
    AP009048 Genomic DNA. Translation: BAA15529.1 .
    PIRi D64933.
    RefSeqi NP_416254.1. NC_000913.3.
    YP_490001.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WXE X-ray 1.90 A 1-275 [» ]
    1WXF X-ray 2.30 A 1-275 [» ]
    1WXG X-ray 1.90 A 1-275 [» ]
    1WXH X-ray 1.90 A 1-275 [» ]
    1WXI X-ray 1.70 A 1-275 [» ]
    ProteinModelPortali P18843.
    SMRi P18843. Positions 2-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10295N.
    IntActi P18843. 480 interactions.
    MINTi MINT-1232772.
    STRINGi 511145.b1740.

    Chemistry

    DrugBanki DB00131. Adenosine monophosphate.

    2D gel databases

    SWISS-2DPAGE P18843.

    Proteomic databases

    PaxDbi P18843.
    PRIDEi P18843.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74810 ; AAC74810 ; b1740 .
    BAA15529 ; BAA15529 ; BAA15529 .
    GeneIDi 12933235.
    946946.
    KEGGi ecj:Y75_p1715.
    eco:b1740.
    PATRICi 32118789. VBIEscCol129921_1812.

    Organism-specific databases

    EchoBASEi EB0657.
    EcoGenei EG10663. nadE.

    Phylogenomic databases

    eggNOGi COG0171.
    HOGENOMi HOG000238070.
    KOi K01916.
    OMAi GQILREM.
    OrthoDBi EOG64JFM7.
    PhylomeDBi P18843.

    Enzyme and pathway databases

    UniPathwayi UPA00253 ; UER00333 .
    BioCyci EcoCyc:NAD-SYNTH-MONOMER.
    ECOL316407:JW1729-MONOMER.
    MetaCyc:NAD-SYNTH-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P18843.
    PROi P18843.

    Gene expression databases

    Genevestigatori P18843.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00193. NadE.
    InterProi IPR022310. NAD/GMP_synthase.
    IPR003694. NAD_synthase.
    IPR022926. NH(3)-dep_NAD(+)_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF02540. NAD_synthase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00552. nadE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complementation of nitrogen-regulatory (ntr-like) mutations in Rhodobacter capsulatus by an Escherichia coli gene: cloning and sequencing of the gene and characterization of the gene product."
      Allibert P., Willison J.C., Vignais P.M.
      J. Bacteriol. 169:260-271(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-12.
      Strain: K12 / EMG2.
    6. "An essential gene (efg) located at 38.1 minutes on the Escherichia coli chromosome."
      Willison J.C.
      J. Bacteriol. 174:5765-5766(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE MAPPING.
    7. "The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase."
      Willison J.C., Tissot G.
      J. Bacteriol. 176:3400-3402(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. "Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements."
      Jauch R., Humm A., Huber R., Wahl M.C.
      J. Biol. Chem. 280:15131-15140(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF APOPROTEIN AND COMPLEX WITH SUBSTRATES; PRODUCTS AND COFACTOR.

    Entry informationi

    Entry nameiNADE_ECOLI
    AccessioniPrimary (citable) accession number: P18843
    Secondary accession number(s): P78235
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3