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P18826 (KPB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Short name=Phosphorylase kinase alpha M subunit
Gene names
Name:Phka1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1241 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Enzyme regulation

By phosphorylation of various serine residues and by calcium.

Pathway

Glycan biosynthesis; glycogen metabolism.

Subunit structure

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Tissue specificity

Both isoforms are expressed in muscle.

Post-translational modification

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated By similarity.

Involvement in disease

Defects in Phka1 are the cause of phosphorylase kinase deficiency in I-strain mice.

Sequence similarities

Belongs to the phosphorylase b kinase regulatory chain family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glycogen metabolism
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   LigandCalmodulin-binding
   Molecular functionMuscle protein
   PTMLipoprotein
Phosphoprotein
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycogen metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhydrolase activity, hydrolyzing O-glycosyl compounds

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P18826-1)

Also known as: ABC;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P18826-2)

Also known as: AC;

The sequence of this isoform differs from the canonical sequence as follows:
     1026-1042: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12411241Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
PRO_0000057727

Regions

Region811 – 84131Calmodulin-binding Potential
Region1064 – 110441Calmodulin-binding Potential

Amino acid modifications

Modified residue7361Phosphoserine By similarity
Modified residue7591Phosphoserine By similarity
Modified residue9731Phosphoserine; by autocatalysis By similarity
Modified residue9861Phosphoserine; by autocatalysis By similarity
Modified residue10081Phosphoserine; by autocatalysis By similarity
Modified residue10191Phosphoserine; by PKA By similarity
Modified residue10211Phosphoserine By similarity
Modified residue10241Phosphoserine By similarity
Lipidation12381S-farnesyl cysteine By similarity

Natural variations

Alternative sequence1026 – 104217Missing in isoform 2.
VSP_004698

Experimental info

Sequence conflict1091K → P in CAA52687. Ref.1
Sequence conflict3091N → Q in CAA52687. Ref.1
Sequence conflict3091N → Q in AAA39927. Ref.3
Sequence conflict3141E → N in CAA52687. Ref.1
Sequence conflict3141E → N in AAA39927. Ref.3
Sequence conflict7191L → I in CAA52687. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ABC) [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 954FFF4155CF1F3A

FASTA1,241138,825
        10         20         30         40         50         60 
MRSRSNSGVR LDGYARLVHQ TILCHQNPVT GLLPASYDQK DAWVRDNVYS ILAVWGLGLA 

        70         80         90        100        110        120 
YRKNADRDED KAKAYELEQS VVKLMRGLLH CMIRQVDKVE SFKYSQSTKD SLHAKYNTKT 

       130        140        150        160        170        180 
CATVVGDDQW GHLQLDATSV YLLFLAQMTA SGLHIIHSLD EVNFIQNLVF YIEAAYKTAD 

       190        200        210        220        230        240 
FGIWERGDKT NQGISELNAS SVGMAKAALE ALDELDLFGV KGGPQSVIHV LADEVQHCQS 

       250        260        270        280        290        300 
ILNSLLPRAS TSKEVDASLL SVVSFPAFAV EDSHLVELTK QEIITKLQGR YGCCRFLRDG 

       310        320        330        340        350        360 
YKTPKEDPNR LYYEPAELKL FENIECEWPL FWTYFILDGI FSGNVEQVQE YREALDAVLI 

       370        380        390        400        410        420 
KGKNGVPLLP ELYSVPPDRV DEEYQNPHTV DRVPMGKLPH MWGQSLYILG SLMAEGFLAP 

       430        440        450        460        470        480 
GEIDPLNRRF STVPKPDVVV QVSILAETEE IKAILKDKGI DVETIAEVYP IRVQPARILS 

       490        500        510        520        530        540 
HIYSSLGCNS RMKLSGRPYR LMGVLGTSKL YDIRKTIFTF TPQFIDQQQF YLALDNQMIV 

       550        560        570        580        590        600 
EMLRTDLSYL CSRWRMTGQP TITFPISHTM LDEDGTSLNS SILAALRKMQ DGYFGGARIQ 

       610        620        630        640        650        660 
TGKLSEFLTT SCCTHLSFMD PGPEGKLYSE DYDEDYEDDL DSGNWMDSYD STSNARCGDE 

       670        680        690        700        710        720 
VARYLDRLLA HTVPHPKLAP TSRKGGLDRF RAAVQTTCDL MSLVAKAKEL HIQNVHMYLP 

       730        740        750        760        770        780 
TKLFQPSRPS LNLLDSPESP QDSQVPSVHV EVHLPRDQSG EVDFQSLVSQ LKETSSLQEQ 

       790        800        810        820        830        840 
ADILYMLYSM KGPDWNTELY EEGGATVREL LSELYVKVGE IRHWGLIRYI SGILRKKVEA 

       850        860        870        880        890        900 
LDEACTDLLS YQKHLTVGLP PEPREKTISA PLPYEALTKL IDEASEGDMS ISTLTQEIMV 

       910        920        930        940        950        960 
YLAMYMRTQP GLFAEMFRLR IGLIIQVMAT ELAHSLRCSA EEATEGLMNL SPSAMKNLLH 

       970        980        990       1000       1010       1020 
HILSGKEFGV ERSVRPTDSN VSPAISIHEI GAVGATKTER TGIMQLKSEI KQVEFRRLSV 

      1030       1040       1050       1060       1070       1080 
SMESQTSGGH PSGVDLMSPS FLSPAACIAA SSGSFPTVCD HQTSKDSRQG QWQRRRRLDG 

      1090       1100       1110       1120       1130       1140 
ALNRVPIGFY QKVWKILQKC HGLSVEGFVL PSSTTREMTP GEIKFSVHVE SVLNRVPQPE 

      1150       1160       1170       1180       1190       1200 
YRQLLVEAIL VLTMLADIEI HSIGSIIAVE KIVHIANDLF LQEQKTLGAD DTMLAKDPAS 

      1210       1220       1230       1240 
GICTLLYDSA PSGRFGTMTY LSKAAATYVQ EFLPHSLCAM Q 

« Hide

Isoform 2 (AC) [UniParc].

Checksum: C944C375B59FFDD8
Show »

FASTA1,224137,154

References

« Hide 'large scale' references
[1]"Phosphorylase kinase deficiency in I-strain mice is associated with a frameshift mutation in the alpha subunit muscle isoform."
Schneider A., Davidson J.J., Wuellrich A., Kilimann M.W.
Nat. Genet. 5:381-385(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: BALB/c.
Tissue: Skeletal muscle.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"I/Lyn mouse phosphorylase kinase deficiency: mutation disrupts expression of the alpha/alpha'-subunit mRNAs."
Bender P.K., Lalley P.A.
Proc. Natl. Acad. Sci. U.S.A. 86:9996-10000(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 243-579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74616 mRNA. Translation: CAA52687.1.
X73877 Transcribed RNA. Translation: CAA52085.1.
AL732405, AL805925 Genomic DNA. Translation: CAM15410.1.
AL805925, AL732405 Genomic DNA. Translation: CAM17599.1.
M28867 mRNA. Translation: AAA39927.1.
PIRS40528.
RefSeqNP_032858.2. NM_008832.2.
UniGeneMm.212889.
Mm.454789.
Mm.489402.

3D structure databases

ProteinModelPortalP18826.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP18826.

Proteomic databases

PaxDbP18826.
PRIDEP18826.

Protocols and materials databases

DNASU18679.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000052012; ENSMUSP00000061991; ENSMUSG00000034055. [P18826-1]
ENSMUST00000113611; ENSMUSP00000109241; ENSMUSG00000034055. [P18826-2]
GeneID18679.
KEGGmmu:18679.
UCSCuc009tyr.1. mouse. [P18826-1]

Organism-specific databases

CTD5255.
MGIMGI:97576. Phka1.

Phylogenomic databases

eggNOGNOG82518.
GeneTreeENSGT00520000055553.
HOGENOMHOG000231478.
HOVERGENHBG000273.
KOK07190.
OMALYSEDYD.
OrthoDBEOG73V6JF.
TreeFamTF313970.

Enzyme and pathway databases

UniPathwayUPA00163.

Gene expression databases

ArrayExpressP18826.
BgeeP18826.
CleanExMM_PHKA1.
GenevestigatorP18826.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011613. Glyco_hydro_15.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERPTHR10749. PTHR10749. 1 hit.
PfamPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
ProtoNetSearch...

Other

NextBio294706.
PROP18826.
SOURCESearch...

Entry information

Entry nameKPB1_MOUSE
AccessionPrimary (citable) accession number: P18826
Secondary accession number(s): A2AI90
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 27, 2011
Last modified: March 19, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot