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P18826

- KPB1_MOUSE

UniProt

P18826 - KPB1_MOUSE

Protein

Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Gene

Phka1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

    Enzyme regulationi

    By phosphorylation of various serine residues and by calcium.

    Pathwayi

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Muscle protein

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Keywords - Ligandi

    Calmodulin-binding

    Enzyme and pathway databases

    UniPathwayiUPA00163.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
    Short name:
    Phosphorylase kinase alpha M subunit
    Gene namesi
    Name:Phka1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:97576. Phka1.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Phka1 are the cause of phosphorylase kinase deficiency in I-strain mice.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12411241Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoformPRO_0000057727Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei736 – 7361PhosphoserineBy similarity
    Modified residuei759 – 7591PhosphoserineBy similarity
    Modified residuei973 – 9731Phosphoserine; by autocatalysisBy similarity
    Modified residuei986 – 9861Phosphoserine; by autocatalysisBy similarity
    Modified residuei1008 – 10081Phosphoserine; by autocatalysisBy similarity
    Modified residuei1019 – 10191Phosphoserine; by PKABy similarity
    Modified residuei1021 – 10211PhosphoserineBy similarity
    Modified residuei1024 – 10241PhosphoserineBy similarity
    Lipidationi1238 – 12381S-farnesyl cysteineBy similarity

    Post-translational modificationi

    Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.By similarity

    Keywords - PTMi

    Lipoprotein, Phosphoprotein, Prenylation

    Proteomic databases

    MaxQBiP18826.
    PaxDbiP18826.
    PRIDEiP18826.

    PTM databases

    PhosphoSiteiP18826.

    Expressioni

    Tissue specificityi

    Both isoforms are expressed in muscle.

    Gene expression databases

    ArrayExpressiP18826.
    BgeeiP18826.
    CleanExiMM_PHKA1.
    GenevestigatoriP18826.

    Interactioni

    Subunit structurei

    Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

    Structurei

    3D structure databases

    ProteinModelPortaliP18826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni811 – 84131Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni1064 – 110441Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG82518.
    GeneTreeiENSGT00520000055553.
    HOGENOMiHOG000231478.
    HOVERGENiHBG000273.
    KOiK07190.
    OMAiLYSEDYD.
    OrthoDBiEOG73V6JF.
    TreeFamiTF313970.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR011613. Glyco_hydro_15.
    IPR008734. PHK_A/B_su.
    [Graphical view]
    PANTHERiPTHR10749. PTHR10749. 1 hit.
    PfamiPF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P18826-1) [UniParc]FASTAAdd to Basket

    Also known as: ABC

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRSRSNSGVR LDGYARLVHQ TILCHQNPVT GLLPASYDQK DAWVRDNVYS     50
    ILAVWGLGLA YRKNADRDED KAKAYELEQS VVKLMRGLLH CMIRQVDKVE 100
    SFKYSQSTKD SLHAKYNTKT CATVVGDDQW GHLQLDATSV YLLFLAQMTA 150
    SGLHIIHSLD EVNFIQNLVF YIEAAYKTAD FGIWERGDKT NQGISELNAS 200
    SVGMAKAALE ALDELDLFGV KGGPQSVIHV LADEVQHCQS ILNSLLPRAS 250
    TSKEVDASLL SVVSFPAFAV EDSHLVELTK QEIITKLQGR YGCCRFLRDG 300
    YKTPKEDPNR LYYEPAELKL FENIECEWPL FWTYFILDGI FSGNVEQVQE 350
    YREALDAVLI KGKNGVPLLP ELYSVPPDRV DEEYQNPHTV DRVPMGKLPH 400
    MWGQSLYILG SLMAEGFLAP GEIDPLNRRF STVPKPDVVV QVSILAETEE 450
    IKAILKDKGI DVETIAEVYP IRVQPARILS HIYSSLGCNS RMKLSGRPYR 500
    LMGVLGTSKL YDIRKTIFTF TPQFIDQQQF YLALDNQMIV EMLRTDLSYL 550
    CSRWRMTGQP TITFPISHTM LDEDGTSLNS SILAALRKMQ DGYFGGARIQ 600
    TGKLSEFLTT SCCTHLSFMD PGPEGKLYSE DYDEDYEDDL DSGNWMDSYD 650
    STSNARCGDE VARYLDRLLA HTVPHPKLAP TSRKGGLDRF RAAVQTTCDL 700
    MSLVAKAKEL HIQNVHMYLP TKLFQPSRPS LNLLDSPESP QDSQVPSVHV 750
    EVHLPRDQSG EVDFQSLVSQ LKETSSLQEQ ADILYMLYSM KGPDWNTELY 800
    EEGGATVREL LSELYVKVGE IRHWGLIRYI SGILRKKVEA LDEACTDLLS 850
    YQKHLTVGLP PEPREKTISA PLPYEALTKL IDEASEGDMS ISTLTQEIMV 900
    YLAMYMRTQP GLFAEMFRLR IGLIIQVMAT ELAHSLRCSA EEATEGLMNL 950
    SPSAMKNLLH HILSGKEFGV ERSVRPTDSN VSPAISIHEI GAVGATKTER 1000
    TGIMQLKSEI KQVEFRRLSV SMESQTSGGH PSGVDLMSPS FLSPAACIAA 1050
    SSGSFPTVCD HQTSKDSRQG QWQRRRRLDG ALNRVPIGFY QKVWKILQKC 1100
    HGLSVEGFVL PSSTTREMTP GEIKFSVHVE SVLNRVPQPE YRQLLVEAIL 1150
    VLTMLADIEI HSIGSIIAVE KIVHIANDLF LQEQKTLGAD DTMLAKDPAS 1200
    GICTLLYDSA PSGRFGTMTY LSKAAATYVQ EFLPHSLCAM Q 1241
    Length:1,241
    Mass (Da):138,825
    Last modified:July 27, 2011 - v3
    Checksum:i954FFF4155CF1F3A
    GO
    Isoform 2 (identifier: P18826-2) [UniParc]FASTAAdd to Basket

    Also known as: AC

    The sequence of this isoform differs from the canonical sequence as follows:
         1026-1042: Missing.

    Show »
    Length:1,224
    Mass (Da):137,154
    Checksum:iC944C375B59FFDD8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091K → P in CAA52687. (PubMed:8298647)Curated
    Sequence conflicti309 – 3091N → Q in CAA52687. (PubMed:8298647)Curated
    Sequence conflicti309 – 3091N → Q in AAA39927. (PubMed:2602386)Curated
    Sequence conflicti314 – 3141E → N in CAA52687. (PubMed:8298647)Curated
    Sequence conflicti314 – 3141E → N in AAA39927. (PubMed:2602386)Curated
    Sequence conflicti719 – 7191L → I in CAA52687. (PubMed:8298647)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1026 – 104217Missing in isoform 2. 1 PublicationVSP_004698Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74616 mRNA. Translation: CAA52687.1.
    X73877 Transcribed RNA. Translation: CAA52085.1.
    AL732405, AL805925 Genomic DNA. Translation: CAM15410.1.
    AL805925, AL732405 Genomic DNA. Translation: CAM17599.1.
    M28867 mRNA. Translation: AAA39927.1.
    CCDSiCCDS30323.1. [P18826-1]
    PIRiS40528.
    RefSeqiNP_032858.2. NM_008832.2. [P18826-1]
    UniGeneiMm.212889.
    Mm.454789.
    Mm.489402.

    Genome annotation databases

    EnsembliENSMUST00000052012; ENSMUSP00000061991; ENSMUSG00000034055. [P18826-1]
    ENSMUST00000113611; ENSMUSP00000109241; ENSMUSG00000034055. [P18826-2]
    GeneIDi18679.
    KEGGimmu:18679.
    UCSCiuc009tyr.1. mouse. [P18826-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74616 mRNA. Translation: CAA52687.1 .
    X73877 Transcribed RNA. Translation: CAA52085.1 .
    AL732405 , AL805925 Genomic DNA. Translation: CAM15410.1 .
    AL805925 , AL732405 Genomic DNA. Translation: CAM17599.1 .
    M28867 mRNA. Translation: AAA39927.1 .
    CCDSi CCDS30323.1. [P18826-1 ]
    PIRi S40528.
    RefSeqi NP_032858.2. NM_008832.2. [P18826-1 ]
    UniGenei Mm.212889.
    Mm.454789.
    Mm.489402.

    3D structure databases

    ProteinModelPortali P18826.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P18826.

    Proteomic databases

    MaxQBi P18826.
    PaxDbi P18826.
    PRIDEi P18826.

    Protocols and materials databases

    DNASUi 18679.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052012 ; ENSMUSP00000061991 ; ENSMUSG00000034055 . [P18826-1 ]
    ENSMUST00000113611 ; ENSMUSP00000109241 ; ENSMUSG00000034055 . [P18826-2 ]
    GeneIDi 18679.
    KEGGi mmu:18679.
    UCSCi uc009tyr.1. mouse. [P18826-1 ]

    Organism-specific databases

    CTDi 5255.
    MGIi MGI:97576. Phka1.

    Phylogenomic databases

    eggNOGi NOG82518.
    GeneTreei ENSGT00520000055553.
    HOGENOMi HOG000231478.
    HOVERGENi HBG000273.
    KOi K07190.
    OMAi LYSEDYD.
    OrthoDBi EOG73V6JF.
    TreeFami TF313970.

    Enzyme and pathway databases

    UniPathwayi UPA00163 .

    Miscellaneous databases

    NextBioi 294706.
    PROi P18826.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P18826.
    Bgeei P18826.
    CleanExi MM_PHKA1.
    Genevestigatori P18826.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR011613. Glyco_hydro_15.
    IPR008734. PHK_A/B_su.
    [Graphical view ]
    PANTHERi PTHR10749. PTHR10749. 1 hit.
    Pfami PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Phosphorylase kinase deficiency in I-strain mice is associated with a frameshift mutation in the alpha subunit muscle isoform."
      Schneider A., Davidson J.J., Wuellrich A., Kilimann M.W.
      Nat. Genet. 5:381-385(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Strain: BALB/c.
      Tissue: Skeletal muscle.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "I/Lyn mouse phosphorylase kinase deficiency: mutation disrupts expression of the alpha/alpha'-subunit mRNAs."
      Bender P.K., Lalley P.A.
      Proc. Natl. Acad. Sci. U.S.A. 86:9996-10000(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 243-579.

    Entry informationi

    Entry nameiKPB1_MOUSE
    AccessioniPrimary (citable) accession number: P18826
    Secondary accession number(s): A2AI90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3