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P18826

- KPB1_MOUSE

UniProt

P18826 - KPB1_MOUSE

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Protein

Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Gene
Phka1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Enzyme regulationi

By phosphorylation of various serine residues and by calcium.

Pathwayi

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

UniPathwayiUPA00163.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Short name:
Phosphorylase kinase alpha M subunit
Gene namesi
Name:Phka1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:97576. Phka1.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Phka1 are the cause of phosphorylase kinase deficiency in I-strain mice.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12411241Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoformPRO_0000057727Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei736 – 7361Phosphoserine By similarity
Modified residuei759 – 7591Phosphoserine By similarity
Modified residuei973 – 9731Phosphoserine; by autocatalysis By similarity
Modified residuei986 – 9861Phosphoserine; by autocatalysis By similarity
Modified residuei1008 – 10081Phosphoserine; by autocatalysis By similarity
Modified residuei1019 – 10191Phosphoserine; by PKA By similarity
Modified residuei1021 – 10211Phosphoserine By similarity
Modified residuei1024 – 10241Phosphoserine By similarity
Lipidationi1238 – 12381S-farnesyl cysteine By similarity

Post-translational modificationi

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated By similarity.

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP18826.
PaxDbiP18826.
PRIDEiP18826.

PTM databases

PhosphoSiteiP18826.

Expressioni

Tissue specificityi

Both isoforms are expressed in muscle.

Gene expression databases

ArrayExpressiP18826.
BgeeiP18826.
CleanExiMM_PHKA1.
GenevestigatoriP18826.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Structurei

3D structure databases

ProteinModelPortaliP18826.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni811 – 84131Calmodulin-binding Reviewed predictionAdd
BLAST
Regioni1064 – 110441Calmodulin-binding Reviewed predictionAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG82518.
GeneTreeiENSGT00520000055553.
HOGENOMiHOG000231478.
HOVERGENiHBG000273.
KOiK07190.
OMAiLYSEDYD.
OrthoDBiEOG73V6JF.
TreeFamiTF313970.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011613. Glyco_hydro_15.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERiPTHR10749. PTHR10749. 1 hit.
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P18826-1) [UniParc]FASTAAdd to Basket

Also known as: ABC

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRSRSNSGVR LDGYARLVHQ TILCHQNPVT GLLPASYDQK DAWVRDNVYS     50
ILAVWGLGLA YRKNADRDED KAKAYELEQS VVKLMRGLLH CMIRQVDKVE 100
SFKYSQSTKD SLHAKYNTKT CATVVGDDQW GHLQLDATSV YLLFLAQMTA 150
SGLHIIHSLD EVNFIQNLVF YIEAAYKTAD FGIWERGDKT NQGISELNAS 200
SVGMAKAALE ALDELDLFGV KGGPQSVIHV LADEVQHCQS ILNSLLPRAS 250
TSKEVDASLL SVVSFPAFAV EDSHLVELTK QEIITKLQGR YGCCRFLRDG 300
YKTPKEDPNR LYYEPAELKL FENIECEWPL FWTYFILDGI FSGNVEQVQE 350
YREALDAVLI KGKNGVPLLP ELYSVPPDRV DEEYQNPHTV DRVPMGKLPH 400
MWGQSLYILG SLMAEGFLAP GEIDPLNRRF STVPKPDVVV QVSILAETEE 450
IKAILKDKGI DVETIAEVYP IRVQPARILS HIYSSLGCNS RMKLSGRPYR 500
LMGVLGTSKL YDIRKTIFTF TPQFIDQQQF YLALDNQMIV EMLRTDLSYL 550
CSRWRMTGQP TITFPISHTM LDEDGTSLNS SILAALRKMQ DGYFGGARIQ 600
TGKLSEFLTT SCCTHLSFMD PGPEGKLYSE DYDEDYEDDL DSGNWMDSYD 650
STSNARCGDE VARYLDRLLA HTVPHPKLAP TSRKGGLDRF RAAVQTTCDL 700
MSLVAKAKEL HIQNVHMYLP TKLFQPSRPS LNLLDSPESP QDSQVPSVHV 750
EVHLPRDQSG EVDFQSLVSQ LKETSSLQEQ ADILYMLYSM KGPDWNTELY 800
EEGGATVREL LSELYVKVGE IRHWGLIRYI SGILRKKVEA LDEACTDLLS 850
YQKHLTVGLP PEPREKTISA PLPYEALTKL IDEASEGDMS ISTLTQEIMV 900
YLAMYMRTQP GLFAEMFRLR IGLIIQVMAT ELAHSLRCSA EEATEGLMNL 950
SPSAMKNLLH HILSGKEFGV ERSVRPTDSN VSPAISIHEI GAVGATKTER 1000
TGIMQLKSEI KQVEFRRLSV SMESQTSGGH PSGVDLMSPS FLSPAACIAA 1050
SSGSFPTVCD HQTSKDSRQG QWQRRRRLDG ALNRVPIGFY QKVWKILQKC 1100
HGLSVEGFVL PSSTTREMTP GEIKFSVHVE SVLNRVPQPE YRQLLVEAIL 1150
VLTMLADIEI HSIGSIIAVE KIVHIANDLF LQEQKTLGAD DTMLAKDPAS 1200
GICTLLYDSA PSGRFGTMTY LSKAAATYVQ EFLPHSLCAM Q 1241
Length:1,241
Mass (Da):138,825
Last modified:July 27, 2011 - v3
Checksum:i954FFF4155CF1F3A
GO
Isoform 2 (identifier: P18826-2) [UniParc]FASTAAdd to Basket

Also known as: AC

The sequence of this isoform differs from the canonical sequence as follows:
     1026-1042: Missing.

Show »
Length:1,224
Mass (Da):137,154
Checksum:iC944C375B59FFDD8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1026 – 104217Missing in isoform 2. VSP_004698Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091K → P in CAA52687. 1 Publication
Sequence conflicti309 – 3091N → Q in CAA52687. 1 Publication
Sequence conflicti309 – 3091N → Q in AAA39927. 1 Publication
Sequence conflicti314 – 3141E → N in CAA52687. 1 Publication
Sequence conflicti314 – 3141E → N in AAA39927. 1 Publication
Sequence conflicti719 – 7191L → I in CAA52687. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74616 mRNA. Translation: CAA52687.1.
X73877 Transcribed RNA. Translation: CAA52085.1.
AL732405, AL805925 Genomic DNA. Translation: CAM15410.1.
AL805925, AL732405 Genomic DNA. Translation: CAM17599.1.
M28867 mRNA. Translation: AAA39927.1.
CCDSiCCDS30323.1. [P18826-1]
PIRiS40528.
RefSeqiNP_032858.2. NM_008832.2. [P18826-1]
UniGeneiMm.212889.
Mm.454789.
Mm.489402.

Genome annotation databases

EnsembliENSMUST00000052012; ENSMUSP00000061991; ENSMUSG00000034055. [P18826-1]
ENSMUST00000113611; ENSMUSP00000109241; ENSMUSG00000034055. [P18826-2]
GeneIDi18679.
KEGGimmu:18679.
UCSCiuc009tyr.1. mouse. [P18826-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74616 mRNA. Translation: CAA52687.1 .
X73877 Transcribed RNA. Translation: CAA52085.1 .
AL732405 , AL805925 Genomic DNA. Translation: CAM15410.1 .
AL805925 , AL732405 Genomic DNA. Translation: CAM17599.1 .
M28867 mRNA. Translation: AAA39927.1 .
CCDSi CCDS30323.1. [P18826-1 ]
PIRi S40528.
RefSeqi NP_032858.2. NM_008832.2. [P18826-1 ]
UniGenei Mm.212889.
Mm.454789.
Mm.489402.

3D structure databases

ProteinModelPortali P18826.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P18826.

Proteomic databases

MaxQBi P18826.
PaxDbi P18826.
PRIDEi P18826.

Protocols and materials databases

DNASUi 18679.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052012 ; ENSMUSP00000061991 ; ENSMUSG00000034055 . [P18826-1 ]
ENSMUST00000113611 ; ENSMUSP00000109241 ; ENSMUSG00000034055 . [P18826-2 ]
GeneIDi 18679.
KEGGi mmu:18679.
UCSCi uc009tyr.1. mouse. [P18826-1 ]

Organism-specific databases

CTDi 5255.
MGIi MGI:97576. Phka1.

Phylogenomic databases

eggNOGi NOG82518.
GeneTreei ENSGT00520000055553.
HOGENOMi HOG000231478.
HOVERGENi HBG000273.
KOi K07190.
OMAi LYSEDYD.
OrthoDBi EOG73V6JF.
TreeFami TF313970.

Enzyme and pathway databases

UniPathwayi UPA00163 .

Miscellaneous databases

NextBioi 294706.
PROi P18826.
SOURCEi Search...

Gene expression databases

ArrayExpressi P18826.
Bgeei P18826.
CleanExi MM_PHKA1.
Genevestigatori P18826.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011613. Glyco_hydro_15.
IPR008734. PHK_A/B_su.
[Graphical view ]
PANTHERi PTHR10749. PTHR10749. 1 hit.
Pfami PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Phosphorylase kinase deficiency in I-strain mice is associated with a frameshift mutation in the alpha subunit muscle isoform."
    Schneider A., Davidson J.J., Wuellrich A., Kilimann M.W.
    Nat. Genet. 5:381-385(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: BALB/c.
    Tissue: Skeletal muscle.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "I/Lyn mouse phosphorylase kinase deficiency: mutation disrupts expression of the alpha/alpha'-subunit mRNAs."
    Bender P.K., Lalley P.A.
    Proc. Natl. Acad. Sci. U.S.A. 86:9996-10000(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 243-579.

Entry informationi

Entry nameiKPB1_MOUSE
AccessioniPrimary (citable) accession number: P18826
Secondary accession number(s): A2AI90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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