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P18826

- KPB1_MOUSE

UniProt

P18826 - KPB1_MOUSE

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Protein

Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Gene

Phka1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Enzyme regulationi

By phosphorylation of various serine residues and by calcium.

Pathwayi

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiREACT_256123. Glycogen breakdown (glycogenolysis).
UniPathwayiUPA00163.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Short name:
Phosphorylase kinase alpha M subunit
Gene namesi
Name:Phka1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:97576. Phka1.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Phka1 are the cause of phosphorylase kinase deficiency in I-strain mice.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12411241Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoformPRO_0000057727Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei736 – 7361PhosphoserineBy similarity
Modified residuei759 – 7591PhosphoserineBy similarity
Modified residuei973 – 9731Phosphoserine; by autocatalysisBy similarity
Modified residuei986 – 9861Phosphoserine; by autocatalysisBy similarity
Modified residuei1008 – 10081Phosphoserine; by autocatalysisBy similarity
Modified residuei1019 – 10191Phosphoserine; by PKABy similarity
Modified residuei1021 – 10211PhosphoserineBy similarity
Modified residuei1024 – 10241PhosphoserineBy similarity
Lipidationi1238 – 12381S-farnesyl cysteineBy similarity

Post-translational modificationi

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.By similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP18826.
PaxDbiP18826.
PRIDEiP18826.

PTM databases

PhosphoSiteiP18826.

Expressioni

Tissue specificityi

Both isoforms are expressed in muscle.

Gene expression databases

BgeeiP18826.
CleanExiMM_PHKA1.
ExpressionAtlasiP18826. baseline and differential.
GenevestigatoriP18826.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Structurei

3D structure databases

ProteinModelPortaliP18826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni811 – 84131Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni1064 – 110441Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG82518.
GeneTreeiENSGT00520000055553.
HOGENOMiHOG000231478.
HOVERGENiHBG000273.
InParanoidiP18826.
KOiK07190.
OMAiLYSEDYD.
OrthoDBiEOG73V6JF.
TreeFamiTF313970.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011613. Glyco_hydro_15.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERiPTHR10749. PTHR10749. 1 hit.
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P18826-1) [UniParc]FASTAAdd to Basket

Also known as: ABC

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSRSNSGVR LDGYARLVHQ TILCHQNPVT GLLPASYDQK DAWVRDNVYS
60 70 80 90 100
ILAVWGLGLA YRKNADRDED KAKAYELEQS VVKLMRGLLH CMIRQVDKVE
110 120 130 140 150
SFKYSQSTKD SLHAKYNTKT CATVVGDDQW GHLQLDATSV YLLFLAQMTA
160 170 180 190 200
SGLHIIHSLD EVNFIQNLVF YIEAAYKTAD FGIWERGDKT NQGISELNAS
210 220 230 240 250
SVGMAKAALE ALDELDLFGV KGGPQSVIHV LADEVQHCQS ILNSLLPRAS
260 270 280 290 300
TSKEVDASLL SVVSFPAFAV EDSHLVELTK QEIITKLQGR YGCCRFLRDG
310 320 330 340 350
YKTPKEDPNR LYYEPAELKL FENIECEWPL FWTYFILDGI FSGNVEQVQE
360 370 380 390 400
YREALDAVLI KGKNGVPLLP ELYSVPPDRV DEEYQNPHTV DRVPMGKLPH
410 420 430 440 450
MWGQSLYILG SLMAEGFLAP GEIDPLNRRF STVPKPDVVV QVSILAETEE
460 470 480 490 500
IKAILKDKGI DVETIAEVYP IRVQPARILS HIYSSLGCNS RMKLSGRPYR
510 520 530 540 550
LMGVLGTSKL YDIRKTIFTF TPQFIDQQQF YLALDNQMIV EMLRTDLSYL
560 570 580 590 600
CSRWRMTGQP TITFPISHTM LDEDGTSLNS SILAALRKMQ DGYFGGARIQ
610 620 630 640 650
TGKLSEFLTT SCCTHLSFMD PGPEGKLYSE DYDEDYEDDL DSGNWMDSYD
660 670 680 690 700
STSNARCGDE VARYLDRLLA HTVPHPKLAP TSRKGGLDRF RAAVQTTCDL
710 720 730 740 750
MSLVAKAKEL HIQNVHMYLP TKLFQPSRPS LNLLDSPESP QDSQVPSVHV
760 770 780 790 800
EVHLPRDQSG EVDFQSLVSQ LKETSSLQEQ ADILYMLYSM KGPDWNTELY
810 820 830 840 850
EEGGATVREL LSELYVKVGE IRHWGLIRYI SGILRKKVEA LDEACTDLLS
860 870 880 890 900
YQKHLTVGLP PEPREKTISA PLPYEALTKL IDEASEGDMS ISTLTQEIMV
910 920 930 940 950
YLAMYMRTQP GLFAEMFRLR IGLIIQVMAT ELAHSLRCSA EEATEGLMNL
960 970 980 990 1000
SPSAMKNLLH HILSGKEFGV ERSVRPTDSN VSPAISIHEI GAVGATKTER
1010 1020 1030 1040 1050
TGIMQLKSEI KQVEFRRLSV SMESQTSGGH PSGVDLMSPS FLSPAACIAA
1060 1070 1080 1090 1100
SSGSFPTVCD HQTSKDSRQG QWQRRRRLDG ALNRVPIGFY QKVWKILQKC
1110 1120 1130 1140 1150
HGLSVEGFVL PSSTTREMTP GEIKFSVHVE SVLNRVPQPE YRQLLVEAIL
1160 1170 1180 1190 1200
VLTMLADIEI HSIGSIIAVE KIVHIANDLF LQEQKTLGAD DTMLAKDPAS
1210 1220 1230 1240
GICTLLYDSA PSGRFGTMTY LSKAAATYVQ EFLPHSLCAM Q
Length:1,241
Mass (Da):138,825
Last modified:July 27, 2011 - v3
Checksum:i954FFF4155CF1F3A
GO
Isoform 2 (identifier: P18826-2) [UniParc]FASTAAdd to Basket

Also known as: AC

The sequence of this isoform differs from the canonical sequence as follows:
     1026-1042: Missing.

Show »
Length:1,224
Mass (Da):137,154
Checksum:iC944C375B59FFDD8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091K → P in CAA52687. (PubMed:8298647)Curated
Sequence conflicti309 – 3091N → Q in CAA52687. (PubMed:8298647)Curated
Sequence conflicti309 – 3091N → Q in AAA39927. (PubMed:2602386)Curated
Sequence conflicti314 – 3141E → N in CAA52687. (PubMed:8298647)Curated
Sequence conflicti314 – 3141E → N in AAA39927. (PubMed:2602386)Curated
Sequence conflicti719 – 7191L → I in CAA52687. (PubMed:8298647)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1026 – 104217Missing in isoform 2. 1 PublicationVSP_004698Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74616 mRNA. Translation: CAA52687.1.
X73877 Transcribed RNA. Translation: CAA52085.1.
AL732405, AL805925 Genomic DNA. Translation: CAM15410.1.
AL805925, AL732405 Genomic DNA. Translation: CAM17599.1.
M28867 mRNA. Translation: AAA39927.1.
CCDSiCCDS30323.1. [P18826-1]
PIRiS40528.
RefSeqiNP_032858.2. NM_008832.2. [P18826-1]
UniGeneiMm.212889.
Mm.454789.
Mm.489402.

Genome annotation databases

EnsembliENSMUST00000052012; ENSMUSP00000061991; ENSMUSG00000034055. [P18826-1]
ENSMUST00000113611; ENSMUSP00000109241; ENSMUSG00000034055. [P18826-2]
GeneIDi18679.
KEGGimmu:18679.
UCSCiuc009tyr.1. mouse. [P18826-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74616 mRNA. Translation: CAA52687.1 .
X73877 Transcribed RNA. Translation: CAA52085.1 .
AL732405 , AL805925 Genomic DNA. Translation: CAM15410.1 .
AL805925 , AL732405 Genomic DNA. Translation: CAM17599.1 .
M28867 mRNA. Translation: AAA39927.1 .
CCDSi CCDS30323.1. [P18826-1 ]
PIRi S40528.
RefSeqi NP_032858.2. NM_008832.2. [P18826-1 ]
UniGenei Mm.212889.
Mm.454789.
Mm.489402.

3D structure databases

ProteinModelPortali P18826.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P18826.

Proteomic databases

MaxQBi P18826.
PaxDbi P18826.
PRIDEi P18826.

Protocols and materials databases

DNASUi 18679.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052012 ; ENSMUSP00000061991 ; ENSMUSG00000034055 . [P18826-1 ]
ENSMUST00000113611 ; ENSMUSP00000109241 ; ENSMUSG00000034055 . [P18826-2 ]
GeneIDi 18679.
KEGGi mmu:18679.
UCSCi uc009tyr.1. mouse. [P18826-1 ]

Organism-specific databases

CTDi 5255.
MGIi MGI:97576. Phka1.

Phylogenomic databases

eggNOGi NOG82518.
GeneTreei ENSGT00520000055553.
HOGENOMi HOG000231478.
HOVERGENi HBG000273.
InParanoidi P18826.
KOi K07190.
OMAi LYSEDYD.
OrthoDBi EOG73V6JF.
TreeFami TF313970.

Enzyme and pathway databases

UniPathwayi UPA00163 .
Reactomei REACT_256123. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi 294706.
PROi P18826.
SOURCEi Search...

Gene expression databases

Bgeei P18826.
CleanExi MM_PHKA1.
ExpressionAtlasi P18826. baseline and differential.
Genevestigatori P18826.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011613. Glyco_hydro_15.
IPR008734. PHK_A/B_su.
[Graphical view ]
PANTHERi PTHR10749. PTHR10749. 1 hit.
Pfami PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Phosphorylase kinase deficiency in I-strain mice is associated with a frameshift mutation in the alpha subunit muscle isoform."
    Schneider A., Davidson J.J., Wuellrich A., Kilimann M.W.
    Nat. Genet. 5:381-385(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: BALB/c.
    Tissue: Skeletal muscle.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "I/Lyn mouse phosphorylase kinase deficiency: mutation disrupts expression of the alpha/alpha'-subunit mRNAs."
    Bender P.K., Lalley P.A.
    Proc. Natl. Acad. Sci. U.S.A. 86:9996-10000(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 243-579.

Entry informationi

Entry nameiKPB1_MOUSE
AccessioniPrimary (citable) accession number: P18826
Secondary accession number(s): A2AI90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3