Reviewed, UniProtKB/Swiss-Prot P18819 (DHE4_EMENI)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:	gdhA
ORF Names:	AN4376

Organism

Emericella nidulans (Aspergillus nidulans)

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	NADP-specific glutamate dehydrogenase		PRO_0000182785
Sites
	Active site	114	1	By similarity
Experimental info
	Sequence conflict	206	1	E → Q in CAA34252. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P18819-1 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: 6825E0399C056F7D	FASTA	459	49,609
	10 20 30 40 50 60 MSNLPVEPEF EQAYKELAST LENSTLFEQH PEYRRALQVV SVPERVIQFR VVWENDKGEV 70 80 90 100 110 120 QINRGYRVQF NSALGPYKGG LRFHPSVNLS ILKFLGFEQI FKNALTGLNM GGGKGGSDFD 130 140 150 160 170 180 PKGKSDSEIR RFCTAFMTEL CKHIGADTDV PAGDIGVTGR EVGFLFGQYR RIRNQWEGVL 190 200 210 220 230 240 TGKGGSWGGS LIRPEATGYG VVYYVEHMIK HVTGGKESFA GKRVAISGSG NVAQYAALKV 250 260 270 280 290 300 IELGGSVVSL SDSKGSLIVK DESASFTPEE IALIADLKVA RKQLSELATS SAFAGKFTYI 310 320 330 340 350 360 PDARPWTNIP GKFEVALPSA TQNEVSGEEA EHLIKSGVRY IAEGSNMGCT QAAIDIFEAH 370 380 390 400 410 420 RNANPGDAIW YAPGKAANAG GVAVSGLEMA QNSARLSWTS EEVDARLKGI MEDCFKNGLE 430 440 450 TAQKFATPAK GVLPSLVTGS NIAGFTKVAE AMKDQGDWW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and regulation of expression of the Aspergillus nidulans gdhA gene encoding NADP dependent glutamate dehydrogenase." Hawkins A.R., Gurr S.J., Montague P., Kinghorn J.R. Mol. Gen. Genet. 218:105-111(1989) [PubMed: 2550758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16121 Genomic DNA. Translation: CAA34252.1. AACD01000076 Genomic DNA. Translation: EAA60293.1.
PIR	S04904.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 3859.
Family and domain databases
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_EMENI

Accession

Primary (citable) accession number: P18819
Secondary accession number(s): Q5B504

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	May 26, 2009
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)