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Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141PROSITE-ProRule annotation

GO - Molecular functioni

  1. glutamate dehydrogenase (NADP+) activity Source: ASPGD

GO - Biological processi

  1. cellular amino acid metabolic process Source: InterPro
  2. cellular response to farnesol Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Alternative name(s):
NADP-dependent glutamate dehydrogenase
Gene namesi
Name:gdhA
ORF Names:AN4376
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome III

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: ASPGD
  2. extracellular region Source: ASPGD
  3. intracellular Source: ASPGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459NADP-specific glutamate dehydrogenasePRO_0000182785Add
BLAST

Proteomic databases

PRIDEiP18819.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi162425.CADANIAP00006082.

Structurei

3D structure databases

ProteinModelPortaliP18819.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243799.
InParanoidiP18819.
KOiK00262.
OMAiKGKSEHE.
OrthoDBiEOG7XSTPW.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18819-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNLPVEPEF EQAYKELAST LENSTLFEQH PEYRRALQVV SVPERVIQFR
60 70 80 90 100
VVWENDKGEV QINRGYRVQF NSALGPYKGG LRFHPSVNLS ILKFLGFEQI
110 120 130 140 150
FKNALTGLNM GGGKGGSDFD PKGKSDSEIR RFCTAFMTEL CKHIGADTDV
160 170 180 190 200
PAGDIGVTGR EVGFLFGQYR RIRNQWEGVL TGKGGSWGGS LIRPEATGYG
210 220 230 240 250
VVYYVEHMIK HVTGGKESFA GKRVAISGSG NVAQYAALKV IELGGSVVSL
260 270 280 290 300
SDSKGSLIVK DESASFTPEE IALIADLKVA RKQLSELATS SAFAGKFTYI
310 320 330 340 350
PDARPWTNIP GKFEVALPSA TQNEVSGEEA EHLIKSGVRY IAEGSNMGCT
360 370 380 390 400
QAAIDIFEAH RNANPGDAIW YAPGKAANAG GVAVSGLEMA QNSARLSWTS
410 420 430 440 450
EEVDARLKGI MEDCFKNGLE TAQKFATPAK GVLPSLVTGS NIAGFTKVAE

AMKDQGDWW
Length:459
Mass (Da):49,609
Last modified:May 26, 2009 - v2
Checksum:i6825E0399C056F7D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti206 – 2061E → Q in CAA34252. (PubMed:2550758)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16121 Genomic DNA. Translation: CAA34252.1.
AACD01000076 Genomic DNA. Translation: EAA60293.1.
BN001303 Genomic DNA. Translation: CBF77653.1.
PIRiS04904.
RefSeqiXP_661980.1. XM_656888.1.

Genome annotation databases

EnsemblFungiiCADANIAT00006082; CADANIAP00006082; CADANIAG00006082.
GeneIDi2872176.
KEGGiani:AN4376.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16121 Genomic DNA. Translation: CAA34252.1.
AACD01000076 Genomic DNA. Translation: EAA60293.1.
BN001303 Genomic DNA. Translation: CBF77653.1.
PIRiS04904.
RefSeqiXP_661980.1. XM_656888.1.

3D structure databases

ProteinModelPortaliP18819.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi162425.CADANIAP00006082.

Proteomic databases

PRIDEiP18819.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00006082; CADANIAP00006082; CADANIAG00006082.
GeneIDi2872176.
KEGGiani:AN4376.2.

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243799.
InParanoidiP18819.
KOiK00262.
OMAiKGKSEHE.
OrthoDBiEOG7XSTPW.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and regulation of expression of the Aspergillus nidulans gdhA gene encoding NADP dependent glutamate dehydrogenase."
    Hawkins A.R., Gurr S.J., Montague P., Kinghorn J.R.
    Mol. Gen. Genet. 218:105-111(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiDHE4_EMENI
AccessioniPrimary (citable) accession number: P18819
Secondary accession number(s): C8V8W7, Q5B504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 26, 2009
Last modified: January 7, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.