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Protein

Methane monooxygenase component A beta chain

Gene

mmoY

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.

Catalytic activityi

Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

GO - Molecular functioni

  • methane monooxygenase activity Source: JCVI

GO - Biological processi

  • cellular aromatic compound metabolic process Source: InterPro
  • methane metabolic process Source: JCVI
  • one-carbon metabolic process Source: UniProtKB-KW
  • oxidation-reduction process Source: JCVI

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciMCAP243233:G1G0X-1194-MONOMER
MetaCyc:MONOMER-3862
BRENDAi1.14.13.25 3305

Names & Taxonomyi

Protein namesi
Recommended name:
Methane monooxygenase component A beta chain (EC:1.14.13.25)
Alternative name(s):
Methane hydroxylase
Gene namesi
Name:mmoY
Ordered Locus Names:MCA1195
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
Proteomesi

Subcellular locationi

GO - Cellular componenti

  • methane monooxygenase complex Source: JCVI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000964072 – 389Methane monooxygenase component A beta chainAdd BLAST388

Interactioni

Subunit structurei

M.capsulatus has two forms of methane monooxygenase, a soluble and a membrane-bound type. The soluble type consists of four components (A to D): protein A, comprising three chains, in an alpha-2, beta-2, gamma-2 configuration, is a nonheme iron protein containing an unusual mu-hydroxo bridge structure at its active site and interacts with both oxygen and methane.

Binary interactionsi

WithEntry#Exp.IntActNotes
mmoXP228695EBI-9023802,EBI-9023796

Protein-protein interaction databases

DIPiDIP-59862N
IntActiP18798, 1 interactor
STRINGi243233.MCA1195

Structurei

Secondary structure

1389
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni10 – 12Combined sources3
Helixi14 – 23Combined sources10
Turni35 – 38Combined sources4
Beta strandi42 – 45Combined sources4
Helixi48 – 53Combined sources6
Helixi85 – 87Combined sources3
Helixi95 – 97Combined sources3
Helixi106 – 129Combined sources24
Helixi132 – 135Combined sources4
Helixi138 – 142Combined sources5
Helixi143 – 147Combined sources5
Helixi148 – 161Combined sources14
Helixi164 – 169Combined sources6
Helixi173 – 203Combined sources31
Helixi212 – 220Combined sources9
Beta strandi222 – 224Combined sources3
Helixi225 – 236Combined sources12
Helixi241 – 250Combined sources10
Helixi252 – 262Combined sources11
Helixi265 – 268Combined sources4
Helixi269 – 272Combined sources4
Helixi276 – 299Combined sources24
Helixi300 – 304Combined sources5
Turni307 – 309Combined sources3
Helixi310 – 335Combined sources26
Helixi336 – 341Combined sources6
Helixi349 – 366Combined sources18
Helixi368 – 371Combined sources4
Helixi377 – 385Combined sources9
Turni386 – 388Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FYZX-ray2.15C/D1-389[»]
1FZ0X-ray2.07C/D1-389[»]
1FZ1X-ray1.96C/D1-389[»]
1FZ2X-ray2.15C/D1-389[»]
1FZ3X-ray2.03C/D1-389[»]
1FZ4X-ray2.38C/D1-389[»]
1FZ5X-ray2.40C/D1-389[»]
1FZ6X-ray2.05C/D1-389[»]
1FZ7X-ray1.96C/D1-389[»]
1FZ8X-ray2.10C/D1-389[»]
1FZ9X-ray2.30C/D1-389[»]
1FZHX-ray2.60C/D1-389[»]
1FZIX-ray3.30C/D1-389[»]
1MMOX-ray2.20B/C6-389[»]
1MTYX-ray1.70B/C6-362[»]
1XMFX-ray2.32C/D2-389[»]
1XMGX-ray2.10C/D2-389[»]
1XMHX-ray2.32C/D2-389[»]
1XU3X-ray2.30C/D1-389[»]
1XU5X-ray1.96C/D1-389[»]
1XVBX-ray1.80C/D1-389[»]
1XVCX-ray2.00C/D1-389[»]
1XVDX-ray2.30C/D1-389[»]
1XVEX-ray2.40C/D1-389[»]
1XVFX-ray2.00C/D1-389[»]
1XVGX-ray1.96C/D1-389[»]
4GAMX-ray2.90B/G/L/Q1-389[»]
ProteinModelPortaliP18798
SMRiP18798
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18798

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105CKQ Bacteria
ENOG410XP26 LUCA
HOGENOMiHOG000086315
KOiK16158
OMAiKFHGGRP
OrthoDBiPOG091H11FY

Family and domain databases

CDDicd01058 AAMH_B, 1 hit
Gene3Di1.10.620.20, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR012078 MP_mOase_hydro
IPR003430 Phenol_Hydrox
IPR012348 RNR-like
PfamiView protein in Pfam
PF02332 Phenol_Hydrox, 1 hit
PIRSFiPIRSF000040 MMOH_comp, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMLGERRRG LTDPEMAAVI LKALPEAPLD GNNKMGYFVT PRWKRLTEYE
60 70 80 90 100
ALTVYAQPNA DWIAGGLDWG DWTQKFHGGR PSWGNETTEL RTVDWFKHRD
110 120 130 140 150
PLRRWHAPYV KDKAEEWRYT DRFLQGYSAD GQIRAMNPTW RDEFINRYWG
160 170 180 190 200
AFLFNEYGLF NAHSQGAREA LSDVTRVSLA FWGFDKIDIA QMIQLERGFL
210 220 230 240 250
AKIVPGFDES TAVPKAEWTN GEVYKSARLA VEGLWQEVFD WNESAFSVHA
260 270 280 290 300
VYDALFGQFV RREFFQRLAP RFGDNLTPFF INQAQTYFQI AKQGVQDLYY
310 320 330 340 350
NCLGDDPEFS DYNRTVMRNW TGKWLEPTIA ALRDFMGLFA KLPAGTTDKE
360 370 380
EITASLYRVV DDWIEDYASR IDFKADRDQI VKAVLAGLK
Length:389
Mass (Da):45,133
Last modified:January 23, 2007 - v4
Checksum:i1737FEA30EEA9875
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti370R → A in AAB62393 (PubMed:2505721).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90050 Genomic DNA Translation: AAB62393.2
AE017282 Genomic DNA Translation: AAU92727.1
PIRiA61412
JL0101
RefSeqiWP_010960483.1, NC_002977.6

Genome annotation databases

EnsemblBacteriaiAAU92727; AAU92727; MCA1195
KEGGimca:MCA1195

Similar proteinsi

Entry informationi

Entry nameiMEMB_METCA
AccessioniPrimary (citable) accession number: P18798
Secondary accession number(s): Q609N7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 134 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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