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Protein

Methane monooxygenase regulatory protein B

Gene

mmoB

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The B protein acts as a regulator of electron flow through the soluble mmo complex, switching the enzyme from an oxidase to a hydroxylase in the presence of the substrate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3865.

Names & Taxonomyi

Protein namesi
Recommended name:
Methane monooxygenase regulatory protein B
Gene namesi
Name:mmoB
Ordered Locus Names:MCA1196
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
ProteomesiUP000006821 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 141141Methane monooxygenase regulatory protein BPRO_0000096507Add
BLAST

Interactioni

Subunit structurei

The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD).

Protein-protein interaction databases

DIPiDIP-60130N.
STRINGi243233.MCA1196.

Structurei

Secondary structure

1
141
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Turni11 – 144Combined sources
Helixi18 – 258Combined sources
Helixi28 – 303Combined sources
Beta strandi37 – 437Combined sources
Helixi46 – 549Combined sources
Turni55 – 584Combined sources
Helixi59 – 635Combined sources
Beta strandi69 – 724Combined sources
Beta strandi74 – 8815Combined sources
Helixi89 – 968Combined sources
Helixi102 – 1054Combined sources
Beta strandi108 – 12821Combined sources
Turni130 – 1323Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CKVNMR-A1-141[»]
4GAMX-ray2.90D/I/N/S1-141[»]
ProteinModelPortaliP18797.
SMRiP18797. Positions 1-141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18797.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG249775.
HOGENOMiHOG000086316.
KOiK16160.
OMAiITSELMG.
OrthoDBiEOG6J48RG.

Family and domain databases

Gene3Di3.90.56.10. 1 hit.
InterProiIPR003454. mOase_MmoB_DmpM.
[Graphical view]
PfamiPF02406. MmoB_DmpM. 1 hit.
[Graphical view]
SUPFAMiSSF56029. SSF56029. 1 hit.

Sequencei

Sequence statusi: Complete.

P18797-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVNSNAYDA GIMGLKGKDF ADQFFADENQ VVHESDTVVL VLKKSDEINT
60 70 80 90 100
FIEEILLTDY KKNVNPTVNV EDRAGYWWIK ANGKIEVDCD EISELLGRQF
110 120 130 140
NVYDFLVDVS STIGRAYTLG NKFTITSELM GLDRKLEDYH A
Length:141
Mass (Da):15,984
Last modified:December 7, 2004 - v2
Checksum:i86A1496846C5840F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121I → F (PubMed:2505721).Curated
Sequence conflicti12 – 121I → F (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90050 Genomic DNA. Translation: AAF04158.2.
AE017282 Genomic DNA. Translation: AAU92726.1.
PIRiJL0103.
RefSeqiWP_010960484.1. NC_002977.6.
YP_113661.1. NC_002977.6.

Genome annotation databases

EnsemblBacteriaiAAU92726; AAU92726; MCA1196.
KEGGimca:MCA1196.
PATRICi22606224. VBIMetCap22254_1228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90050 Genomic DNA. Translation: AAF04158.2.
AE017282 Genomic DNA. Translation: AAU92726.1.
PIRiJL0103.
RefSeqiWP_010960484.1. NC_002977.6.
YP_113661.1. NC_002977.6.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CKVNMR-A1-141[»]
4GAMX-ray2.90D/I/N/S1-141[»]
ProteinModelPortaliP18797.
SMRiP18797. Positions 1-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60130N.
STRINGi243233.MCA1196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU92726; AAU92726; MCA1196.
KEGGimca:MCA1196.
PATRICi22606224. VBIMetCap22254_1228.

Phylogenomic databases

eggNOGiNOG249775.
HOGENOMiHOG000086316.
KOiK16160.
OMAiITSELMG.
OrthoDBiEOG6J48RG.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3865.

Miscellaneous databases

EvolutionaryTraceiP18797.

Family and domain databases

Gene3Di3.90.56.10. 1 hit.
InterProiIPR003454. mOase_MmoB_DmpM.
[Graphical view]
PfamiPF02406. MmoB_DmpM. 1 hit.
[Graphical view]
SUPFAMiSSF56029. SSF56029. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of methane monooxygenase from Methylococcus capsulatus (Bath)."
    Stainthorpe A.C., Murrell J.C., Salmond G.P.C., Dalton H., Lees V.
    Arch. Microbiol. 152:154-159(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 33009 / NCIMB 11132 / Bath.
  2. McDonald I., Murrell J.C.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33009 / NCIMB 11132 / Bath.
  4. "Identification of the gene encoding the regulatory protein B of soluble methane monooxygenase."
    Pilkington S.J., Salmond G.P.C., Murrell J.C., Dalton H.
    FEMS Microbiol. Lett. 72:345-348(1990)
    Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 3-19.
  5. "Structure of the soluble methane monooxygenase regulatory protein B."
    Walters K.J., Gassner G.T., Lippard S.J., Wagner G.
    Proc. Natl. Acad. Sci. U.S.A. 96:7877-7882(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  6. "Mutational and structural analyses of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath)."
    Brandstetter H., Whittington D.A., Lippard S.J., Frederick C.A.
    Chem. Biol. 6:441-449(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CIRCULAR DICHROISM ANALYSIS, 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiMMOB_METCA
AccessioniPrimary (citable) accession number: P18797
Secondary accession number(s): Q609N6, Q9LCT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 7, 2004
Last modified: May 27, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.