P18797 (MMOB_METCA) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 73.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Methane monooxygenase regulatory protein B | ||||
| Gene names |
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| Organism | Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 243233 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus |
Protein attributes
| Sequence length | 141 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | The B protein acts as a regulator of electron flow through the soluble mmo complex, switching the enzyme from an oxidase to a hydroxylase in the presence of the substrate. |
| Subunit structure | The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/mmoX, beta/mmoY and gamma/mmoZ), B/MMOB (mmoB), C/MMOR (mmoC) and D/MMOD (mmoD). |
Ontologies
| Keywords | |
|---|---|
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | 3D-structure Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 141 | 141 | Methane monooxygenase regulatory protein B | PRO_0000096507 | |||||||||||||||||||||||||
Experimental info | |||||||||||||||||||||||||||||
| Sequence conflict | 12 | 1 | I → F Ref.1 | ||||||||||||||||||||||||||
| Sequence conflict | 12 | 1 | I → F Ref.4 | ||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||
| Turn | 9 – 17 | 9 | |||||||||||||||||||||||||||
| Turn | 22 – 24 | 3 | |||||||||||||||||||||||||||
| Beta strand | 38 – 42 | 5 | |||||||||||||||||||||||||||
| Helix | 46 – 63 | 18 | |||||||||||||||||||||||||||
| Beta strand | 69 – 72 | 4 | |||||||||||||||||||||||||||
| Beta strand | 74 – 87 | 14 | |||||||||||||||||||||||||||
| Helix | 91 – 95 | 5 | |||||||||||||||||||||||||||
| Turn | 96 – 99 | 4 | |||||||||||||||||||||||||||
| Turn | 103 – 106 | 4 | |||||||||||||||||||||||||||
| Beta strand | 107 – 118 | 12 | |||||||||||||||||||||||||||
| Beta strand | 123 – 127 | 5 | |||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular analysis of methane monooxygenase from Methylococcus capsulatus (Bath)." Stainthorpe A.C., Murrell J.C., Salmond G.P.C., Dalton H., Lees V. Arch. Microbiol. 152:154-159(1989) [PubMed: 2505721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath. |
| [2] | McDonald I., Murrell J.C. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. Eisen J.A.PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath. |
| [4] | "Identification of the gene encoding the regulatory protein B of soluble methane monooxygenase." Pilkington S.J., Salmond G.P.C., Murrell J.C., Dalton H. FEMS Microbiol. Lett. 72:345-348(1990) Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 3-19. |
| [5] | "Structure of the soluble methane monooxygenase regulatory protein B." Walters K.J., Gassner G.T., Lippard S.J., Wagner G. Proc. Natl. Acad. Sci. U.S.A. 96:7877-7882(1999) [PubMed: 10393915] [Abstract] Cited for: STRUCTURE BY NMR. |
| [6] | "Mutational and structural analyses of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath)." Brandstetter H., Whittington D.A., Lippard S.J., Frederick C.A. Chem. Biol. 6:441-449(1999) [PubMed: 10381404] [Abstract] Cited for: CIRCULAR DICHROISM ANALYSIS, 3D-STRUCTURE MODELING. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | M90050 Genomic DNA. Translation: AAF04158.2. AE017282 Genomic DNA. Translation: AAU92726.1. | ||||||||||||
| PIR | JL0103. | ||||||||||||
| RefSeq | YP_113661.1. NC_002977.6. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | P18797. | ||||||||||||
| SMR | P18797. Positions 1-141. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| GeneID | 3103283. | ||||||||||||
| GenomeReviews | Gene locus MCA1196 in contig AE017282_GR. | ||||||||||||
| KEGG | mca:MCA1196. | ||||||||||||
| NMPDR | fig|243233.4.peg.363. | ||||||||||||
| PATRIC | 22606224. VBIMetCap22254_1228. | ||||||||||||
| TIGR | MCA1196. | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | HBG553991. | ||||||||||||
| OMA | STIGRAY. | ||||||||||||
| ProtClustDB | CLSK2395508. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BioCyc | MCAP243233:MCA_1196-MONOMER. MetaCyc:MONOMER-3865. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR003454. mOase_MmoB_DmpM. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.90.56.10. mOase_MmoB_DmpM. 1 hit. | ||||||||||||
| KO | K08684. | ||||||||||||
| Pfam | PF02406. MmoB_DmpM. 1 hit. [Graphical view] | ||||||||||||
| SUPFAM | SSF56029. MmoB_DmpM. 1 hit. | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | MMOB_METCA | ||||||||
| Accession | Primary (citable) accession number: P18797 Secondary accession number(s): Q609N6, Q9LCT7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |

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