P18797 (MMOB_METCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methane monooxygenase regulatory protein B

Gene names

Name:	mmoB
Ordered Locus Names:	MCA1196

Organism

Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]

Taxonomic identifier

243233 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The B protein acts as a regulator of electron flow through the soluble mmo complex, switching the enzyme from an oxidase to a hydroxylase in the presence of the substrate.
Subunit structure	The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/mmoX, beta/mmoY and gamma/mmoZ), B/MMOB (mmoB), C/MMOR (mmoC) and D/MMOD (mmoD).

Ontologies

Keywords
Molecular function	Monooxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 141

141

Methane monooxygenase regulatory protein B

PRO_0000096507

Experimental info

Sequence conflict

12

1

I → F Ref.1

Sequence conflict

12

1

I → F Ref.4

Secondary structure

1

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141

Helix Strand Turn

Details...

Turn

9 – 17

9

Turn

22 – 24

3

Beta strand

38 – 42

5

Helix

46 – 63

18

Beta strand

69 – 72

4

Beta strand

74 – 87

14

Helix

91 – 95

5

Turn

96 – 99

4

Turn

103 – 106

4

Beta strand

107 – 118

12

Beta strand

123 – 127

5

Sequences

	Sequence		Length	Mass (Da)
	P18797 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 86A1496846C5840F	FASTA	141	15,984
	10 20 30 40 50 60 MSVNSNAYDA GIMGLKGKDF ADQFFADENQ VVHESDTVVL VLKKSDEINT FIEEILLTDY 70 80 90 100 110 120 KKNVNPTVNV EDRAGYWWIK ANGKIEVDCD EISELLGRQF NVYDFLVDVS STIGRAYTLG 130 140 NKFTITSELM GLDRKLEDYH A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of methane monooxygenase from Methylococcus capsulatus (Bath)." Stainthorpe A.C., Murrell J.C., Salmond G.P.C., Dalton H., Lees V. Arch. Microbiol. 152:154-159(1989) [PubMed: 2505721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath.
[2]	McDonald I., Murrell J.C. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)." Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A. PLoS Biol. 2:1616-1628(2004) [PubMed: 15383840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33009 / NCIMB 11132 / Bath.
[4]	"Identification of the gene encoding the regulatory protein B of soluble methane monooxygenase." Pilkington S.J., Salmond G.P.C., Murrell J.C., Dalton H. FEMS Microbiol. Lett. 72:345-348(1990) Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 3-19.
[5]	"Structure of the soluble methane monooxygenase regulatory protein B." Walters K.J., Gassner G.T., Lippard S.J., Wagner G. Proc. Natl. Acad. Sci. U.S.A. 96:7877-7882(1999) [PubMed: 10393915] [Abstract] Cited for: STRUCTURE BY NMR.
[6]	"Mutational and structural analyses of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath)." Brandstetter H., Whittington D.A., Lippard S.J., Frederick C.A. Chem. Biol. 6:441-449(1999) [PubMed: 10381404] [Abstract] Cited for: CIRCULAR DICHROISM ANALYSIS, 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M90050 Genomic DNA. Translation: AAF04158.2.
AE017282 Genomic DNA. Translation: AAU92726.1.

PIR

JL0103.

RefSeq

YP_113661.1. NC_002977.6.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CKV	NMR	-	A	1-141	[»]

ProteinModelPortal

P18797.

SMR

P18797. Positions 1-141.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3103283.

GenomeReviews

Gene locus MCA1196 in contig AE017282_GR.

KEGG

mca:MCA1196.

NMPDR

fig|243233.4.peg.363.

PATRIC

22606224. VBIMetCap22254_1228.

TIGR

MCA1196.

Phylogenomic databases

HOGENOM

HBG553991.

OMA

STIGRAY.

ProtClustDB

CLSK2395508.

Enzyme and pathway databases

BioCyc

MCAP243233:MCA_1196-MONOMER.
MetaCyc:MONOMER-3865.

Family and domain databases

InterPro

IPR003454. mOase_MmoB_DmpM.
[Graphical view]

Gene3D

G3DSA:3.90.56.10. mOase_MmoB_DmpM. 1 hit.

KO

K08684.

Pfam

PF02406. MmoB_DmpM. 1 hit.
[Graphical view]

SUPFAM

SSF56029. MmoB_DmpM. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MMOB_METCA

Accession

Primary (citable) accession number: P18797
Secondary accession number(s): Q609N6, Q9LCT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program