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P18797

- MMOB_METCA

UniProt

P18797 - MMOB_METCA

Protein

Methane monooxygenase regulatory protein B

Gene

mmoB

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    The B protein acts as a regulator of electron flow through the soluble mmo complex, switching the enzyme from an oxidase to a hydroxylase in the presence of the substrate.

    GO - Molecular functioni

    1. monooxygenase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular aromatic compound metabolic process Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-3865.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methane monooxygenase regulatory protein B
    Gene namesi
    Name:mmoB
    Ordered Locus Names:MCA1196
    OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
    Taxonomic identifieri243233 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
    ProteomesiUP000006821: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 141141Methane monooxygenase regulatory protein BPRO_0000096507Add
    BLAST

    Interactioni

    Subunit structurei

    The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD).

    Protein-protein interaction databases

    DIPiDIP-60130N.
    STRINGi243233.MCA1196.

    Structurei

    Secondary structure

    1
    141
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Turni11 – 144
    Helixi18 – 258
    Helixi28 – 303
    Beta strandi37 – 437
    Helixi46 – 549
    Turni55 – 584
    Helixi59 – 635
    Beta strandi69 – 724
    Beta strandi74 – 8815
    Helixi89 – 968
    Helixi102 – 1054
    Beta strandi108 – 12821
    Turni130 – 1323

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CKVNMR-A1-141[»]
    4GAMX-ray2.90D/I/N/S1-141[»]
    ProteinModelPortaliP18797.
    SMRiP18797. Positions 1-141.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18797.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG249775.
    HOGENOMiHOG000086316.
    KOiK16160.
    OMAiSTIGRAY.
    OrthoDBiEOG6J48RG.

    Family and domain databases

    Gene3Di3.90.56.10. 1 hit.
    InterProiIPR003454. mOase_MmoB_DmpM.
    [Graphical view]
    PfamiPF02406. MmoB_DmpM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56029. SSF56029. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18797-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVNSNAYDA GIMGLKGKDF ADQFFADENQ VVHESDTVVL VLKKSDEINT    50
    FIEEILLTDY KKNVNPTVNV EDRAGYWWIK ANGKIEVDCD EISELLGRQF 100
    NVYDFLVDVS STIGRAYTLG NKFTITSELM GLDRKLEDYH A 141
    Length:141
    Mass (Da):15,984
    Last modified:December 7, 2004 - v2
    Checksum:i86A1496846C5840F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121I → F(PubMed:2505721)Curated
    Sequence conflicti12 – 121I → F1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90050 Genomic DNA. Translation: AAF04158.2.
    AE017282 Genomic DNA. Translation: AAU92726.1.
    PIRiJL0103.
    RefSeqiWP_010960484.1. NC_002977.6.
    YP_113661.1. NC_002977.6.

    Genome annotation databases

    EnsemblBacteriaiAAU92726; AAU92726; MCA1196.
    GeneIDi3103283.
    KEGGimca:MCA1196.
    PATRICi22606224. VBIMetCap22254_1228.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90050 Genomic DNA. Translation: AAF04158.2 .
    AE017282 Genomic DNA. Translation: AAU92726.1 .
    PIRi JL0103.
    RefSeqi WP_010960484.1. NC_002977.6.
    YP_113661.1. NC_002977.6.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CKV NMR - A 1-141 [» ]
    4GAM X-ray 2.90 D/I/N/S 1-141 [» ]
    ProteinModelPortali P18797.
    SMRi P18797. Positions 1-141.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60130N.
    STRINGi 243233.MCA1196.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU92726 ; AAU92726 ; MCA1196 .
    GeneIDi 3103283.
    KEGGi mca:MCA1196.
    PATRICi 22606224. VBIMetCap22254_1228.

    Phylogenomic databases

    eggNOGi NOG249775.
    HOGENOMi HOG000086316.
    KOi K16160.
    OMAi STIGRAY.
    OrthoDBi EOG6J48RG.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-3865.

    Miscellaneous databases

    EvolutionaryTracei P18797.

    Family and domain databases

    Gene3Di 3.90.56.10. 1 hit.
    InterProi IPR003454. mOase_MmoB_DmpM.
    [Graphical view ]
    Pfami PF02406. MmoB_DmpM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56029. SSF56029. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of methane monooxygenase from Methylococcus capsulatus (Bath)."
      Stainthorpe A.C., Murrell J.C., Salmond G.P.C., Dalton H., Lees V.
      Arch. Microbiol. 152:154-159(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33009 / NCIMB 11132 / Bath.
    2. McDonald I., Murrell J.C.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33009 / NCIMB 11132 / Bath.
    4. "Identification of the gene encoding the regulatory protein B of soluble methane monooxygenase."
      Pilkington S.J., Salmond G.P.C., Murrell J.C., Dalton H.
      FEMS Microbiol. Lett. 72:345-348(1990)
      Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 3-19.
    5. "Structure of the soluble methane monooxygenase regulatory protein B."
      Walters K.J., Gassner G.T., Lippard S.J., Wagner G.
      Proc. Natl. Acad. Sci. U.S.A. 96:7877-7882(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    6. "Mutational and structural analyses of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath)."
      Brandstetter H., Whittington D.A., Lippard S.J., Frederick C.A.
      Chem. Biol. 6:441-449(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CIRCULAR DICHROISM ANALYSIS, 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiMMOB_METCA
    AccessioniPrimary (citable) accession number: P18797
    Secondary accession number(s): Q609N6, Q9LCT7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3