Reviewed, UniProtKB/Swiss-Prot P18773 (EST_ACILW)

Names and origin

Protein names

Recommended name:
    Esterase
    EC=3.1.1.-

Gene names

Name:

est

Organism

Acinetobacter lwoffii

Taxonomic identifier

28090 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Subcellular location	Secreted Potential. Note: External to the cytoplasmic membrane.
Miscellaneous	Acinetobacter lwoffii RAG-1 has the ability to grow on simple triglycerides such as triacetin; mutants defective in esterase lose this ability, but retain the ability to grow on acetate.
Sequence similarities	Belongs to the 'GDXG' lipolytic enzyme family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Serine esterase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Esterase		PRO_0000071554
Sites
	Active site	79	1	Potential
	Active site	149	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P18773-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 3B038A5A15BA81B3	FASTA	303	33,911
	10 20 30 40 50 60 MKFGTVWKYY FTESLLKATI RTPSQLNLAP NALRPVLDQL CRLFPQNPTV QIRPIRLAGV 70 80 90 100 110 120 RGEEIKAQAS ATQLIFHIHG GAFFLGSLNT HRALMTDLAS RTQMQVIHVD YPLAPEHPYP 130 140 150 160 170 180 EAIDAIFDVY QALLVQGIKP KDIIISGDSC GANLALALSL RLKQQPELMP SGLILMSPYL 190 200 210 220 230 240 DLTLTSESLR FNQKHDALLS IEALQAGIKH YLTDDIQPGD PRVSPLFDDL DGLPPTLVQV 250 260 270 280 290 300 GSKEILLDDS KRFREKAEQA DVKVHFKLYT GMWNNFQMFN AWFPEAKQAL ADIAEFATSL DLD

References

[1]	"Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinetobacter calcoaceticus RAG-1." Reddy P.G., Allon R., Mevarech M., Mendelovitz S., Sato Y., Gutnick D.L. Gene 76:145-152(1989) [PubMed: 2545531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RAG-1 / ATCC 31012.
[2]	Gutnick D.L. Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M24890 Genomic DNA. Translation: AAA02895.1.
PIR	JS0202.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013094. AB_hydrolase_3. IPR002168. Lipase_GDXG_AS. [Graphical view]
Pfam	PF07859. Abhydrolase_3. 1 hit. [Graphical view]
PROSITE	PS01173. LIPASE_GDXG_HIS. 1 hit. PS01174. LIPASE_GDXG_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EST_ACILW

Accession

Primary (citable) accession number: P18773

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)