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Protein

Esterase

Gene

est

Organism
Acinetobacter lwoffii
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491PROSITE-ProRule annotationBy similarity
Active sitei244 – 2441By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

ESTHERiacica-este1. Hormone-sensitive_lipase_like_1.

Names & Taxonomyi

Protein namesi
Recommended name:
Esterase (EC:3.1.1.-)
Gene namesi
Name:est
OrganismiAcinetobacter lwoffii
Taxonomic identifieri28090 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

Subcellular locationi

  • Secreted Curated

  • Note: External to the cytoplasmic membrane.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303EsterasePRO_0000071554Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP18773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi79 – 813Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity

Sequence similaritiesi

Belongs to the 'GDXG' lipolytic enzyme family.Curated

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR002168. Lipase_GDXG_HIS_AS.
IPR033140. Lipase_GDXG_put_SER_AS.
[Graphical view]
PfamiPF07859. Abhydrolase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01173. LIPASE_GDXG_HIS. 1 hit.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFGTVWKYY FTESLLKATI RTPSQLNLAP NALRPVLDQL CRLFPQNPTV
60 70 80 90 100
QIRPIRLAGV RGEEIKAQAS ATQLIFHIHG GAFFLGSLNT HRALMTDLAS
110 120 130 140 150
RTQMQVIHVD YPLAPEHPYP EAIDAIFDVY QALLVQGIKP KDIIISGDSC
160 170 180 190 200
GANLALALSL RLKQQPELMP SGLILMSPYL DLTLTSESLR FNQKHDALLS
210 220 230 240 250
IEALQAGIKH YLTDDIQPGD PRVSPLFDDL DGLPPTLVQV GSKEILLDDS
260 270 280 290 300
KRFREKAEQA DVKVHFKLYT GMWNNFQMFN AWFPEAKQAL ADIAEFATSL

DLD
Length:303
Mass (Da):33,911
Last modified:February 1, 1996 - v2
Checksum:i3B038A5A15BA81B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24890 Genomic DNA. Translation: AAA02895.1.
PIRiJS0202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24890 Genomic DNA. Translation: AAA02895.1.
PIRiJS0202.

3D structure databases

ProteinModelPortaliP18773.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERiacica-este1. Hormone-sensitive_lipase_like_1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR002168. Lipase_GDXG_HIS_AS.
IPR033140. Lipase_GDXG_put_SER_AS.
[Graphical view]
PfamiPF07859. Abhydrolase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01173. LIPASE_GDXG_HIS. 1 hit.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinetobacter calcoaceticus RAG-1."
    Reddy P.G., Allon R., Mevarech M., Mendelovitz S., Sato Y., Gutnick D.L.
    Gene 76:145-152(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RAG-1 / ATCC 31012.
  2. Gutnick D.L.
    Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiEST_ACILW
AccessioniPrimary (citable) accession number: P18773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Acinetobacter lwoffii RAG-1 has the ability to grow on simple triglycerides such as triacetin; mutants defective in esterase lose this ability, but retain the ability to grow on acetate.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.