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Protein

Cofilin-1

Gene

Cfl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11809832). Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity). Required for neural tube morphogenesis and neural crest cell migration (PubMed:15649475).By similarity2 Publications

GO - Molecular functioni

  • actin filament binding Source: UniProtKB
  • receptor binding Source: ARUK-UCL

GO - Biological processi

  • actin filament depolymerization Source: UniProtKB
  • actin filament fragmentation Source: UniProtKB
  • actin filament organization Source: MGI
  • cell projection organization Source: MGI
  • cytoskeleton organization Source: UniProtKB
  • establishment of cell polarity Source: MGI
  • mitotic cytokinesis Source: MGI
  • negative regulation of cell size Source: MGI
  • neural crest cell migration Source: MGI
  • neural fold formation Source: MGI
  • platelet degranulation Source: Reactome
  • positive regulation by host of viral process Source: MGI
  • positive regulation of actin filament depolymerization Source: CACAO
  • protein phosphorylation Source: MGI
  • regulation of cell morphogenesis Source: UniProtKB
  • regulation of dendritic spine morphogenesis Source: ParkinsonsUK-UCL
  • response to amino acid Source: MGI
  • response to virus Source: Ensembl

Keywordsi

Molecular functionActin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cofilin-1
Alternative name(s):
Cofilin, non-muscle isoform
Gene namesi
Name:Cfl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:101757. Cfl1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002149002 – 166Cofilin-1Add BLAST165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei3PhosphoserineCombined sources1 Publication1
Modified residuei8PhosphoserineCombined sources1
Modified residuei13N6-acetyllysineBy similarity1
Modified residuei25PhosphothreonineBy similarity1
Modified residuei41PhosphoserineBy similarity1
Modified residuei68PhosphotyrosineCombined sources1
Modified residuei73N6-acetyllysineBy similarity1
Cross-linki132Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei140PhosphotyrosineCombined sources1
Modified residuei144N6-acetyllysineBy similarity1
Modified residuei156PhosphoserineCombined sources1

Post-translational modificationi

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (PubMed:25107909). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2 (By similarity).By similarity1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP18760.
PaxDbiP18760.
PeptideAtlasiP18760.
PRIDEiP18760.
TopDownProteomicsiP18760.

2D gel databases

REPRODUCTION-2DPAGEiP18760.
SWISS-2DPAGEiP18760.

PTM databases

iPTMnetiP18760.
PhosphoSitePlusiP18760.
SwissPalmiP18760.

Expressioni

Tissue specificityi

Widely distributed in various tissues. Not found in skeletal muscle.1 Publication

Developmental stagei

In E10.5 embryo somites is expressed in a ventral cell layer (myotome).1 Publication

Gene expression databases

CleanExiMM_CFL1.
ExpressionAtlasiP18760. baseline and differential.

Interactioni

Subunit structurei

Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.By similarity

GO - Molecular functioni

  • actin filament binding Source: UniProtKB
  • receptor binding Source: ARUK-UCL

Protein-protein interaction databases

BioGridi198684. 8 interactors.
DIPiDIP-38073N.
IntActiP18760. 12 interactors.
MINTiMINT-1862314.
STRINGi10090.ENSMUSP00000112259.

Structurei

3D structure databases

ProteinModelPortaliP18760.
SMRiP18760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 153ADF-HPROSITE-ProRule annotationAdd BLAST150

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi30 – 34Nuclear localization signalSequence analysis5

Sequence similaritiesi

Belongs to the actin-binding proteins ADF family.Curated

Phylogenomic databases

eggNOGiKOG1735. Eukaryota.
ENOG41122P5. LUCA.
GeneTreeiENSGT00440000033289.
HOGENOMiHOG000039697.
HOVERGENiHBG000381.
InParanoidiP18760.
KOiK05765.
PhylomeDBiP18760.

Family and domain databases

CDDicd11286. ADF_cofilin_like. 1 hit.
Gene3Di3.40.20.10. 1 hit.
InterProiView protein in InterPro
IPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom_sf.
IPR017904. ADF/Cofilin.
IPR027234. Cofilin_1.
PANTHERiPTHR11913. PTHR11913. 1 hit.
PTHR11913:SF17. PTHR11913:SF17. 1 hit.
PfamiView protein in Pfam
PF00241. Cofilin_ADF. 1 hit.
PRINTSiPR00006. COFILIN.
SMARTiView protein in SMART
SM00102. ADF. 1 hit.
PROSITEiView protein in PROSITE
PS51263. ADF_H. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE
60 70 80 90 100
EGKEILVGDV GQTVDDPYTT FVKMLPDKDC RYALYDATYE TKESKKEDLV
110 120 130 140 150
FIFWAPENAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA
160
EKLGGSAVIS LEGKPL
Length:166
Mass (Da):18,560
Last modified:January 23, 2007 - v3
Checksum:i19834E8CA80747B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00472 mRNA. Translation: BAA00364.1.
BC046225 mRNA. Translation: AAH46225.1.
BC058726 mRNA. Translation: AAH58726.1.
CCDSiCCDS84409.1.
PIRiS12584.
RefSeqiNP_031713.1. NM_007687.5.
UniGeneiMm.329655.

Genome annotation databases

EnsembliENSMUST00000209469; ENSMUSP00000147514; ENSMUSG00000056201.
GeneIDi12631.
KEGGimmu:12631.
UCSCiuc008gdq.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiCOF1_MOUSE
AccessioniPrimary (citable) accession number: P18760
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 178 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families