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Protein

Vesicular-fusion protein SEC18

Gene

SEC18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi281 – 288ATPSequence analysis8
Nucleotide bindingi564 – 571ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • phosphatidic acid binding Source: SGD
  • SNARE binding Source: SGD

GO - Biological processi

  • autophagosome assembly Source: SGD
  • Golgi to plasma membrane protein transport Source: SGD
  • Golgi vesicle docking Source: SGD
  • inter-Golgi cisterna vesicle-mediated transport Source: SGD
  • macroautophagy Source: SGD
  • SNARE complex disassembly Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vesicle fusion with Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29048-MONOMER.
ReactomeiR-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811438. Intra-Golgi traffic.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Protein family/group databases

TCDBi1.F.1.1.2. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular-fusion protein SEC18
Gene namesi
Name:SEC18
Ordered Locus Names:YBR080C
ORF Names:YBR0736
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR080C.
SGDiS000000284. SEC18.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • Golgi stack Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845711 – 758Vesicular-fusion protein SEC18Add BLAST758

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18759.
PRIDEiP18759.

PTM databases

iPTMnetiP18759.

Interactioni

Subunit structurei

Homohexamer. Binds to SEC17.

GO - Molecular functioni

  • SNARE binding Source: SGD

Protein-protein interaction databases

BioGridi32782. 137 interactors.
DIPiDIP-2497N.
IntActiP18759. 15 interactors.
MINTiMINT-707733.

Structurei

Secondary structure

1758
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 33Combined sources7
Helixi37 – 40Combined sources4
Turni41 – 43Combined sources3
Beta strandi44 – 47Combined sources4
Turni49 – 51Combined sources3
Beta strandi57 – 60Combined sources4
Turni61 – 63Combined sources3
Beta strandi64 – 70Combined sources7
Beta strandi78 – 81Combined sources4
Helixi83 – 89Combined sources7
Beta strandi96 – 101Combined sources6
Helixi104 – 108Combined sources5
Beta strandi113 – 123Combined sources11
Helixi136 – 147Combined sources12
Beta strandi157 – 162Combined sources6
Beta strandi165 – 177Combined sources13
Beta strandi181 – 183Combined sources3
Beta strandi203 – 208Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CR5X-ray2.30A/B/C22-210[»]
ProteinModelPortaliP18759.
SMRiP18759.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18759.

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
InParanoidiP18759.
KOiK06027.
OMAiFRRAFNS.
OrthoDBiEOG092C0YZZ.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKIPGFGKA AANHTPPDMT NMDTRTRHLK VSNCPNNSYA LANVAAVSPN
60 70 80 90 100
DFPNNIYIII DNLFVFTTRH SNDIPPGTIG FNGNQRTWGG WSLNQDVQAK
110 120 130 140 150
AFDLFKYSGK QSYLGSIDID ISFRARGKAV STVFDQDELA KQFVRCYESQ
160 170 180 190 200
IFSPTQYLIM EFQGHFFDLK IRNVQAIDLG DIEPTSAVAT GIETKGILTK
210 220 230 240 250
QTQINFFKGR DGLVNLKSSN SLRPRSNAVI RPDFKFEDLG VGGLDKEFTK
260 270 280 290 300
IFRRAFASRI FPPSVIEKLG ISHVKGLLLY GPPGTGKTLI ARKIGTMLNA
310 320 330 340 350
KEPKIVNGPE ILSKYVGSSE ENIRNLFKDA EAEYRAKGEE SSLHIIIFDE
360 370 380 390 400
LDSVFKQRGS RGDGTGVGDN VVNQLLAKMD GVDQLNNILV IGMTNRKDLI
410 420 430 440 450
DSALLRPGRF EVQVEIHLPD EKGRLQIFDI QTKKMRENNM MSDDVNLAEL
460 470 480 490 500
AALTKNFSGA EIEGLVKSAS SFAINKTVNI GKGATKLNTK DIAKLKVTRE
510 520 530 540 550
DFLNALNDVT PAFGISEEDL KTCVEGGMML YSERVNSILK NGARYVRQVR
560 570 580 590 600
ESDKSRLVSL LIHGPAGSGK TALAAEIALK SGFPFIRLIS PNELSGMSES
610 620 630 640 650
AKIAYIDNTF RDAYKSPLNI LVIDSLETLV DWVPIGPRFS NNILQMLKVA
660 670 680 690 700
LKRKPPQDRR LLIMTTTSAY SVLQQMDILS CFDNEIAVPN MTNLDELNNV
710 720 730 740 750
MIESNFLDDA GRVKVINELS RSCPNFNVGI KKTLTNIETA RHDEDPVNEL

VELMTQSA
Length:758
Mass (Da):84,056
Last modified:October 1, 1994 - v2
Checksum:iAD6CE77EA3674B3C
GO

Sequence cautioni

The sequence AAA35031 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti381Missing in AAA35030 (PubMed:3054509).Curated1
Sequence conflicti381Missing in AAA35031 (PubMed:3054509).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20662 Genomic DNA. Translation: AAA35030.1.
M20662 Genomic DNA. Translation: AAA35031.1. Different initiation.
X76294 Genomic DNA. Translation: CAA53939.1.
Z35949 Genomic DNA. Translation: CAA85025.1.
AF004912 Genomic DNA. Translation: AAB82417.1.
BK006936 Genomic DNA. Translation: DAA07199.1.
PIRiS45477.
RefSeqiNP_009636.3. NM_001178428.3.

Genome annotation databases

EnsemblFungiiYBR080C; YBR080C; YBR080C.
GeneIDi852372.
KEGGisce:YBR080C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20662 Genomic DNA. Translation: AAA35030.1.
M20662 Genomic DNA. Translation: AAA35031.1. Different initiation.
X76294 Genomic DNA. Translation: CAA53939.1.
Z35949 Genomic DNA. Translation: CAA85025.1.
AF004912 Genomic DNA. Translation: AAB82417.1.
BK006936 Genomic DNA. Translation: DAA07199.1.
PIRiS45477.
RefSeqiNP_009636.3. NM_001178428.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CR5X-ray2.30A/B/C22-210[»]
ProteinModelPortaliP18759.
SMRiP18759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32782. 137 interactors.
DIPiDIP-2497N.
IntActiP18759. 15 interactors.
MINTiMINT-707733.

Protein family/group databases

TCDBi1.F.1.1.2. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

iPTMnetiP18759.

Proteomic databases

MaxQBiP18759.
PRIDEiP18759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR080C; YBR080C; YBR080C.
GeneIDi852372.
KEGGisce:YBR080C.

Organism-specific databases

EuPathDBiFungiDB:YBR080C.
SGDiS000000284. SEC18.

Phylogenomic databases

GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
InParanoidiP18759.
KOiK06027.
OMAiFRRAFNS.
OrthoDBiEOG092C0YZZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29048-MONOMER.
ReactomeiR-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811438. Intra-Golgi traffic.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

EvolutionaryTraceiP18759.
PROiP18759.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEC18_YEAST
AccessioniPrimary (citable) accession number: P18759
Secondary accession number(s): D6VQ79, Q07067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5920 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.