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P18759

- SEC18_YEAST

UniProt

P18759 - SEC18_YEAST

Protein

Vesicular-fusion protein SEC18

Gene

SEC18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi281 – 2888ATPSequence Analysis
    Nucleotide bindingi564 – 5718ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. autophagic vacuole assembly Source: SGD
    3. Golgi to plasma membrane protein transport Source: SGD
    4. Golgi vesicle docking Source: SGD
    5. inter-Golgi cisterna vesicle-mediated transport Source: SGD
    6. SNARE complex disassembly Source: SGD
    7. vacuole fusion, non-autophagic Source: SGD
    8. vesicle fusion with Golgi apparatus Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29048-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicular-fusion protein SEC18
    Gene namesi
    Name:SEC18
    Ordered Locus Names:YBR080C
    ORF Names:YBR0736
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR080c.
    SGDiS000000284. SEC18.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 758758Vesicular-fusion protein SEC18PRO_0000084571Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei226 – 2261Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP18759.
    PaxDbiP18759.
    PRIDEiP18759.

    Expressioni

    Gene expression databases

    GenevestigatoriP18759.

    Interactioni

    Subunit structurei

    Homohexamer. Binds to SEC17.

    Protein-protein interaction databases

    BioGridi32782. 129 interactions.
    DIPiDIP-2497N.
    IntActiP18759. 13 interactions.
    MINTiMINT-707733.
    STRINGi4932.YBR080C.

    Structurei

    Secondary structure

    1
    758
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi27 – 337
    Helixi37 – 404
    Turni41 – 433
    Beta strandi44 – 474
    Turni49 – 513
    Beta strandi57 – 604
    Turni61 – 633
    Beta strandi64 – 707
    Beta strandi78 – 814
    Helixi83 – 897
    Beta strandi96 – 1016
    Helixi104 – 1085
    Beta strandi113 – 12311
    Helixi136 – 14712
    Beta strandi157 – 1626
    Beta strandi165 – 17713
    Beta strandi181 – 1833
    Beta strandi203 – 2086

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CR5X-ray2.30A/B/C22-210[»]
    ProteinModelPortaliP18759.
    SMRiP18759. Positions 26-210, 231-740.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18759.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00530000064085.
    HOGENOMiHOG000198544.
    KOiK06027.
    OMAiFKDKERS.
    OrthoDBiEOG72ZCPJ.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18759-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKIPGFGKA AANHTPPDMT NMDTRTRHLK VSNCPNNSYA LANVAAVSPN    50
    DFPNNIYIII DNLFVFTTRH SNDIPPGTIG FNGNQRTWGG WSLNQDVQAK 100
    AFDLFKYSGK QSYLGSIDID ISFRARGKAV STVFDQDELA KQFVRCYESQ 150
    IFSPTQYLIM EFQGHFFDLK IRNVQAIDLG DIEPTSAVAT GIETKGILTK 200
    QTQINFFKGR DGLVNLKSSN SLRPRSNAVI RPDFKFEDLG VGGLDKEFTK 250
    IFRRAFASRI FPPSVIEKLG ISHVKGLLLY GPPGTGKTLI ARKIGTMLNA 300
    KEPKIVNGPE ILSKYVGSSE ENIRNLFKDA EAEYRAKGEE SSLHIIIFDE 350
    LDSVFKQRGS RGDGTGVGDN VVNQLLAKMD GVDQLNNILV IGMTNRKDLI 400
    DSALLRPGRF EVQVEIHLPD EKGRLQIFDI QTKKMRENNM MSDDVNLAEL 450
    AALTKNFSGA EIEGLVKSAS SFAINKTVNI GKGATKLNTK DIAKLKVTRE 500
    DFLNALNDVT PAFGISEEDL KTCVEGGMML YSERVNSILK NGARYVRQVR 550
    ESDKSRLVSL LIHGPAGSGK TALAAEIALK SGFPFIRLIS PNELSGMSES 600
    AKIAYIDNTF RDAYKSPLNI LVIDSLETLV DWVPIGPRFS NNILQMLKVA 650
    LKRKPPQDRR LLIMTTTSAY SVLQQMDILS CFDNEIAVPN MTNLDELNNV 700
    MIESNFLDDA GRVKVINELS RSCPNFNVGI KKTLTNIETA RHDEDPVNEL 750
    VELMTQSA 758
    Length:758
    Mass (Da):84,056
    Last modified:October 1, 1994 - v2
    Checksum:iAD6CE77EA3674B3C
    GO

    Sequence cautioni

    The sequence AAA35031.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti381 – 3811Missing in AAA35030. (PubMed:3054509)Curated
    Sequence conflicti381 – 3811Missing in AAA35031. (PubMed:3054509)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20662 Genomic DNA. Translation: AAA35030.1.
    M20662 Genomic DNA. Translation: AAA35031.1. Different initiation.
    X76294 Genomic DNA. Translation: CAA53939.1.
    Z35949 Genomic DNA. Translation: CAA85025.1.
    AF004912 Genomic DNA. Translation: AAB82417.1.
    BK006936 Genomic DNA. Translation: DAA07199.1.
    PIRiS45477.
    RefSeqiNP_009636.3. NM_001178428.3.

    Genome annotation databases

    EnsemblFungiiYBR080C; YBR080C; YBR080C.
    GeneIDi852372.
    KEGGisce:YBR080C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20662 Genomic DNA. Translation: AAA35030.1 .
    M20662 Genomic DNA. Translation: AAA35031.1 . Different initiation.
    X76294 Genomic DNA. Translation: CAA53939.1 .
    Z35949 Genomic DNA. Translation: CAA85025.1 .
    AF004912 Genomic DNA. Translation: AAB82417.1 .
    BK006936 Genomic DNA. Translation: DAA07199.1 .
    PIRi S45477.
    RefSeqi NP_009636.3. NM_001178428.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CR5 X-ray 2.30 A/B/C 22-210 [» ]
    ProteinModelPortali P18759.
    SMRi P18759. Positions 26-210, 231-740.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32782. 129 interactions.
    DIPi DIP-2497N.
    IntActi P18759. 13 interactions.
    MINTi MINT-707733.
    STRINGi 4932.YBR080C.

    Proteomic databases

    MaxQBi P18759.
    PaxDbi P18759.
    PRIDEi P18759.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR080C ; YBR080C ; YBR080C .
    GeneIDi 852372.
    KEGGi sce:YBR080C.

    Organism-specific databases

    CYGDi YBR080c.
    SGDi S000000284. SEC18.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00530000064085.
    HOGENOMi HOG000198544.
    KOi K06027.
    OMAi FKDKERS.
    OrthoDBi EOG72ZCPJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29048-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P18759.
    NextBioi 971158.
    PROi P18759.

    Gene expression databases

    Genevestigatori P18759.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product."
      Eakle K.A., Bernstein M., Emr S.D.
      Mol. Cell. Biol. 8:4098-4109(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II."
      van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y.
      Yeast 10:959-964(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Vornlocher H.-P., Hanachi P., Hershey J.W.B.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 566-758.
      Strain: ATCC 200060 / W303.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Crystal structure of the Sec18p N-terminal domain."
      Babor S.M., Fass D.
      Proc. Natl. Acad. Sci. U.S.A. 96:14759-14764(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 22-210.

    Entry informationi

    Entry nameiSEC18_YEAST
    AccessioniPrimary (citable) accession number: P18759
    Secondary accession number(s): D6VQ79, Q07067
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 147 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5920 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3