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Protein

Vesicular-fusion protein SEC18

Gene

SEC18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi281 – 2888ATPSequence Analysis
Nucleotide bindingi564 – 5718ATPSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. autophagic vacuole assembly Source: SGD
  2. Golgi to plasma membrane protein transport Source: SGD
  3. Golgi vesicle docking Source: SGD
  4. inter-Golgi cisterna vesicle-mediated transport Source: SGD
  5. SNARE complex disassembly Source: SGD
  6. vacuole fusion, non-autophagic Source: SGD
  7. vesicle fusion with Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29048-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular-fusion protein SEC18
Gene namesi
Name:SEC18
Ordered Locus Names:YBR080C
ORF Names:YBR0736
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBR080c.
SGDiS000000284. SEC18.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 758758Vesicular-fusion protein SEC18PRO_0000084571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei226 – 2261Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18759.
PaxDbiP18759.
PRIDEiP18759.

Expressioni

Gene expression databases

GenevestigatoriP18759.

Interactioni

Subunit structurei

Homohexamer. Binds to SEC17.

Protein-protein interaction databases

BioGridi32782. 132 interactions.
DIPiDIP-2497N.
IntActiP18759. 15 interactions.
MINTiMINT-707733.
STRINGi4932.YBR080C.

Structurei

Secondary structure

1
758
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 337Combined sources
Helixi37 – 404Combined sources
Turni41 – 433Combined sources
Beta strandi44 – 474Combined sources
Turni49 – 513Combined sources
Beta strandi57 – 604Combined sources
Turni61 – 633Combined sources
Beta strandi64 – 707Combined sources
Beta strandi78 – 814Combined sources
Helixi83 – 897Combined sources
Beta strandi96 – 1016Combined sources
Helixi104 – 1085Combined sources
Beta strandi113 – 12311Combined sources
Helixi136 – 14712Combined sources
Beta strandi157 – 1626Combined sources
Beta strandi165 – 17713Combined sources
Beta strandi181 – 1833Combined sources
Beta strandi203 – 2086Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CR5X-ray2.30A/B/C22-210[»]
ProteinModelPortaliP18759.
SMRiP18759. Positions 26-210, 231-740.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18759.

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
InParanoidiP18759.
KOiK06027.
OMAiFKDKERS.
OrthoDBiEOG72ZCPJ.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKIPGFGKA AANHTPPDMT NMDTRTRHLK VSNCPNNSYA LANVAAVSPN
60 70 80 90 100
DFPNNIYIII DNLFVFTTRH SNDIPPGTIG FNGNQRTWGG WSLNQDVQAK
110 120 130 140 150
AFDLFKYSGK QSYLGSIDID ISFRARGKAV STVFDQDELA KQFVRCYESQ
160 170 180 190 200
IFSPTQYLIM EFQGHFFDLK IRNVQAIDLG DIEPTSAVAT GIETKGILTK
210 220 230 240 250
QTQINFFKGR DGLVNLKSSN SLRPRSNAVI RPDFKFEDLG VGGLDKEFTK
260 270 280 290 300
IFRRAFASRI FPPSVIEKLG ISHVKGLLLY GPPGTGKTLI ARKIGTMLNA
310 320 330 340 350
KEPKIVNGPE ILSKYVGSSE ENIRNLFKDA EAEYRAKGEE SSLHIIIFDE
360 370 380 390 400
LDSVFKQRGS RGDGTGVGDN VVNQLLAKMD GVDQLNNILV IGMTNRKDLI
410 420 430 440 450
DSALLRPGRF EVQVEIHLPD EKGRLQIFDI QTKKMRENNM MSDDVNLAEL
460 470 480 490 500
AALTKNFSGA EIEGLVKSAS SFAINKTVNI GKGATKLNTK DIAKLKVTRE
510 520 530 540 550
DFLNALNDVT PAFGISEEDL KTCVEGGMML YSERVNSILK NGARYVRQVR
560 570 580 590 600
ESDKSRLVSL LIHGPAGSGK TALAAEIALK SGFPFIRLIS PNELSGMSES
610 620 630 640 650
AKIAYIDNTF RDAYKSPLNI LVIDSLETLV DWVPIGPRFS NNILQMLKVA
660 670 680 690 700
LKRKPPQDRR LLIMTTTSAY SVLQQMDILS CFDNEIAVPN MTNLDELNNV
710 720 730 740 750
MIESNFLDDA GRVKVINELS RSCPNFNVGI KKTLTNIETA RHDEDPVNEL

VELMTQSA
Length:758
Mass (Da):84,056
Last modified:September 30, 1994 - v2
Checksum:iAD6CE77EA3674B3C
GO

Sequence cautioni

The sequence AAA35031.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti381 – 3811Missing in AAA35030 (PubMed:3054509).Curated
Sequence conflicti381 – 3811Missing in AAA35031 (PubMed:3054509).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20662 Genomic DNA. Translation: AAA35030.1.
M20662 Genomic DNA. Translation: AAA35031.1. Different initiation.
X76294 Genomic DNA. Translation: CAA53939.1.
Z35949 Genomic DNA. Translation: CAA85025.1.
AF004912 Genomic DNA. Translation: AAB82417.1.
BK006936 Genomic DNA. Translation: DAA07199.1.
PIRiS45477.
RefSeqiNP_009636.3. NM_001178428.3.

Genome annotation databases

EnsemblFungiiYBR080C; YBR080C; YBR080C.
GeneIDi852372.
KEGGisce:YBR080C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20662 Genomic DNA. Translation: AAA35030.1.
M20662 Genomic DNA. Translation: AAA35031.1. Different initiation.
X76294 Genomic DNA. Translation: CAA53939.1.
Z35949 Genomic DNA. Translation: CAA85025.1.
AF004912 Genomic DNA. Translation: AAB82417.1.
BK006936 Genomic DNA. Translation: DAA07199.1.
PIRiS45477.
RefSeqiNP_009636.3. NM_001178428.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CR5X-ray2.30A/B/C22-210[»]
ProteinModelPortaliP18759.
SMRiP18759. Positions 26-210, 231-740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32782. 132 interactions.
DIPiDIP-2497N.
IntActiP18759. 15 interactions.
MINTiMINT-707733.
STRINGi4932.YBR080C.

Proteomic databases

MaxQBiP18759.
PaxDbiP18759.
PRIDEiP18759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR080C; YBR080C; YBR080C.
GeneIDi852372.
KEGGisce:YBR080C.

Organism-specific databases

CYGDiYBR080c.
SGDiS000000284. SEC18.

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
InParanoidiP18759.
KOiK06027.
OMAiFKDKERS.
OrthoDBiEOG72ZCPJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29048-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP18759.
NextBioi971158.
PROiP18759.

Gene expression databases

GenevestigatoriP18759.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product."
    Eakle K.A., Bernstein M., Emr S.D.
    Mol. Cell. Biol. 8:4098-4109(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II."
    van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y.
    Yeast 10:959-964(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Vornlocher H.-P., Hanachi P., Hershey J.W.B.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 566-758.
    Strain: ATCC 200060 / W303.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Crystal structure of the Sec18p N-terminal domain."
    Babor S.M., Fass D.
    Proc. Natl. Acad. Sci. U.S.A. 96:14759-14764(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 22-210.

Entry informationi

Entry nameiSEC18_YEAST
AccessioniPrimary (citable) accession number: P18759
Secondary accession number(s): D6VQ79, Q07067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1990
Last sequence update: September 30, 1994
Last modified: March 3, 2015
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5920 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.