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P18759 (SEC18_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular-fusion protein SEC18
Gene names
Name:SEC18
Ordered Locus Names:YBR080C
ORF Names:YBR0736
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Subunit structure

Homohexamer. Binds to SEC17.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 5920 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the AAA ATPase family.

Sequence caution

The sequence AAA35031.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 758758Vesicular-fusion protein SEC18
PRO_0000084571

Regions

Nucleotide binding281 – 2888ATP Potential
Nucleotide binding564 – 5718ATP Potential

Amino acid modifications

Modified residue2261Phosphoserine Ref.7 Ref.8

Experimental info

Sequence conflict3811Missing in AAA35030. Ref.1
Sequence conflict3811Missing in AAA35031. Ref.1

Secondary structure

................................. 758
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18759 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: AD6CE77EA3674B3C

FASTA75884,056
        10         20         30         40         50         60 
MFKIPGFGKA AANHTPPDMT NMDTRTRHLK VSNCPNNSYA LANVAAVSPN DFPNNIYIII 

        70         80         90        100        110        120 
DNLFVFTTRH SNDIPPGTIG FNGNQRTWGG WSLNQDVQAK AFDLFKYSGK QSYLGSIDID 

       130        140        150        160        170        180 
ISFRARGKAV STVFDQDELA KQFVRCYESQ IFSPTQYLIM EFQGHFFDLK IRNVQAIDLG 

       190        200        210        220        230        240 
DIEPTSAVAT GIETKGILTK QTQINFFKGR DGLVNLKSSN SLRPRSNAVI RPDFKFEDLG 

       250        260        270        280        290        300 
VGGLDKEFTK IFRRAFASRI FPPSVIEKLG ISHVKGLLLY GPPGTGKTLI ARKIGTMLNA 

       310        320        330        340        350        360 
KEPKIVNGPE ILSKYVGSSE ENIRNLFKDA EAEYRAKGEE SSLHIIIFDE LDSVFKQRGS 

       370        380        390        400        410        420 
RGDGTGVGDN VVNQLLAKMD GVDQLNNILV IGMTNRKDLI DSALLRPGRF EVQVEIHLPD 

       430        440        450        460        470        480 
EKGRLQIFDI QTKKMRENNM MSDDVNLAEL AALTKNFSGA EIEGLVKSAS SFAINKTVNI 

       490        500        510        520        530        540 
GKGATKLNTK DIAKLKVTRE DFLNALNDVT PAFGISEEDL KTCVEGGMML YSERVNSILK 

       550        560        570        580        590        600 
NGARYVRQVR ESDKSRLVSL LIHGPAGSGK TALAAEIALK SGFPFIRLIS PNELSGMSES 

       610        620        630        640        650        660 
AKIAYIDNTF RDAYKSPLNI LVIDSLETLV DWVPIGPRFS NNILQMLKVA LKRKPPQDRR 

       670        680        690        700        710        720 
LLIMTTTSAY SVLQQMDILS CFDNEIAVPN MTNLDELNNV MIESNFLDDA GRVKVINELS 

       730        740        750 
RSCPNFNVGI KKTLTNIETA RHDEDPVNEL VELMTQSA 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product."
Eakle K.A., Bernstein M., Emr S.D.
Mol. Cell. Biol. 8:4098-4109(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II."
van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y.
Yeast 10:959-964(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]Vornlocher H.-P., Hanachi P., Hershey J.W.B.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 566-758.
Strain: ATCC 200060 / W303.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Crystal structure of the Sec18p N-terminal domain."
Babor S.M., Fass D.
Proc. Natl. Acad. Sci. U.S.A. 96:14759-14764(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 22-210.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20662 Genomic DNA. Translation: AAA35030.1.
M20662 Genomic DNA. Translation: AAA35031.1. Different initiation.
X76294 Genomic DNA. Translation: CAA53939.1.
Z35949 Genomic DNA. Translation: CAA85025.1.
AF004912 Genomic DNA. Translation: AAB82417.1.
BK006936 Genomic DNA. Translation: DAA07199.1.
PIRS45477.
RefSeqNP_009636.3. NM_001178428.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CR5X-ray2.30A/B/C22-210[»]
ProteinModelPortalP18759.
SMRP18759. Positions 26-210, 231-740.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32782. 129 interactions.
DIPDIP-2497N.
IntActP18759. 13 interactions.
MINTMINT-707733.
STRING4932.YBR080C.

Proteomic databases

MaxQBP18759.
PaxDbP18759.
PRIDEP18759.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR080C; YBR080C; YBR080C.
GeneID852372.
KEGGsce:YBR080C.

Organism-specific databases

CYGDYBR080c.
SGDS000000284. SEC18.

Phylogenomic databases

eggNOGCOG0464.
GeneTreeENSGT00530000064085.
HOGENOMHOG000198544.
KOK06027.
OMAFKDKERS.
OrthoDBEOG72ZCPJ.

Enzyme and pathway databases

BioCycYEAST:G3O-29048-MONOMER.

Gene expression databases

GenevestigatorP18759.

Family and domain databases

Gene3D3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP18759.
NextBio971158.
PROP18759.

Entry information

Entry nameSEC18_YEAST
AccessionPrimary (citable) accession number: P18759
Secondary accession number(s): D6VQ79, Q07067
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references