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Protein

Cystathionine gamma-lyase

Gene

Cth

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function (By similarity).By similarity

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (Cbs)
  2. Cystathionine gamma-lyase (Cth)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611SubstrateBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei338 – 3381SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Calmodulin-binding, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8584.
ReactomeiR-RNO-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
UniPathwayiUPA00136; UER00202.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-lyase (EC:4.4.1.1)
Alternative name(s):
Cysteine-protein sulfhydrase
Gamma-cystathionase
Probasin-related antigen
Short name:
PRB-RA
Gene namesi
Name:Cth
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2443. Cth.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Cystathionine gamma-lyasePRO_0000114752Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineCombined sources
Modified residuei211 – 2111N6-(pyridoxal phosphate)lysine

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP18757.
PRIDEiP18757.

PTM databases

iPTMnetiP18757.
PhosphoSiteiP18757.

Interactioni

Subunit structurei

Homotetramer. Interacts with CALM in a calcium-dependent manner (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4576462.
STRINGi10116.ENSRNOP00000058914.

Structurei

3D structure databases

ProteinModelPortaliP18757.
SMRiP18757. Positions 9-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
HOGENOMiHOG000246415.
HOVERGENiHBG005322.
InParanoidiP18757.
KOiK01758.
PhylomeDBiP18757.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKDASSSGF LPSFQHFATQ AIHVGPEPEQ WSSRAVVLPI SLATTFKQDS
60 70 80 90 100
PGQSSGFVYS RSGNPTRNCL EKAVAALDGA KHCLTFARGL AATTTITHLL
110 120 130 140 150
KAGDEVICMD EVYGGTNRYF RRVASEFGLK ISFVDCSKTK LLEAAITPQT
160 170 180 190 200
KLVWIETPTN PTLKLADIKA CAQIVHKHKD IILVVDNTFM SAYFQRPLAL
210 220 230 240 250
GADICMCSAT KYMNGHSDVV MGLVSVTSDD LNERLRFLQN SLGAVPSPFD
260 270 280 290 300
CYLCCRGLKT LQIRMEKHFR NGMAVARFLE SNPRVEKVIY PGLPSHPQHE
310 320 330 340 350
LAKRQCTGCP GMVSFYIKGT LQHAQVFLKN IKLFALAESL GGYESLAELP
360 370 380 390
AIMTHASVPE KDRATLGISD TLIRLSVGLE DEKDLLEDLG QALKAAHP
Length:398
Mass (Da):43,605
Last modified:October 1, 1996 - v2
Checksum:iC6043988B03D725F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 417AVVLPIS → CCGAAH AA sequence (PubMed:2201285).Curated
Sequence conflicti227 – 2271T → N in CAA37547 (PubMed:2201285).Curated
Sequence conflicti260 – 27112TLQIR…KHFRN → HCRSGWRNTFQD in CAA37547 (PubMed:2201285).CuratedAdd
BLAST
Sequence conflicti305 – 3084QCTG → SARA in CAA37547 (PubMed:2201285).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17370 mRNA. Translation: BAA04189.1.
X53460 mRNA. Translation: CAA37547.1.
PIRiA49864.
RefSeqiNP_058770.1. NM_017074.1.
UniGeneiRn.3881.

Genome annotation databases

GeneIDi24962.
KEGGirno:24962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17370 mRNA. Translation: BAA04189.1.
X53460 mRNA. Translation: CAA37547.1.
PIRiA49864.
RefSeqiNP_058770.1. NM_017074.1.
UniGeneiRn.3881.

3D structure databases

ProteinModelPortaliP18757.
SMRiP18757. Positions 9-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4576462.
STRINGi10116.ENSRNOP00000058914.

PTM databases

iPTMnetiP18757.
PhosphoSiteiP18757.

Proteomic databases

PaxDbiP18757.
PRIDEiP18757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24962.
KEGGirno:24962.

Organism-specific databases

CTDi1491.
RGDi2443. Cth.

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
HOGENOMiHOG000246415.
HOVERGENiHBG005322.
InParanoidiP18757.
KOiK01758.
PhylomeDBiP18757.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00202.
BioCyciMetaCyc:MONOMER-8584.
ReactomeiR-RNO-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

PROiP18757.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCGL_RAT
AccessioniPrimary (citable) accession number: P18757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.