Reviewed, UniProtKB/Swiss-Prot P18708 (NSF_CRIGR)

Names and origin

Protein names

Recommended name:
    Vesicle-fusing ATPase
    EC=3.6.4.6
Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein
      Short name=NEM-sensitive fusion protein
    Vesicular-fusion protein NSF

Gene names

Name:

NSF

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Ref.2
Catalytic activity	ATP + H2O = ADP + phosphate.
Cofactor	Binds 1 magnesium ion per subunit.
Subunit structure	Interacts with GABARAP and GABARAPL2 By similarity. Homohexamer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GABARAPL1	Q9H0R8	1	EBI-925742,EBI-746969	From a different organism.
Gria2	P19491	1	EBI-925742,EBI-77718	From a different organism.
Gria3	P19492	1	EBI-925742,EBI-77764	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 744

744

Vesicle-fusing ATPase

PRO_0000084562

Regions

Nucleotide binding

260 – 267

8

ATP Potential

Nucleotide binding

543 – 550

8

ATP Potential

Sites

Metal binding

550

1

Magnesium

Amino acid modifications

Modified residue

259

1

Phosphotyrosine By similarity

Secondary structure

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744

Helix Strand Turn

Details...

Beta strand

4 – 10

7

Helix

14 – 18

5

Beta strand

22 – 24

3

Turn

26 – 28

3

Beta strand

34 – 40

7

Beta strand

43 – 51

9

Beta strand

59 – 62

4

Helix

64 – 70

7

Beta strand

77 – 83

7

Helix

87 – 90

4

Beta strand

91 – 102

12

Helix

104 – 106

3

Beta strand

111 – 113

3

Helix

114 – 125

12

Beta strand

129 – 131

3

Beta strand

135 – 140

6

Beta strand

143 – 154

12

Beta strand

173 – 176

4

Beta strand

182 – 187

6

Beta strand

194 – 200

7

Turn

500 – 502

3

Helix

512 – 530

19

Beta strand

535 – 542

8

Helix

549 – 560

12

Beta strand

563 – 568

6

Helix

570 – 572

3

Helix

578 – 593

16

Beta strand

595 – 602

8

Helix

605 – 608

4

Turn

613 – 616

4

Helix

620 – 629

10

Beta strand

639 – 647

9

Helix

649 – 654

6

Turn

658 – 660

3

Beta strand

661 – 666

6

Beta strand

670 – 672

3

Helix

673 – 683

11

Helix

688 – 698

11

Beta strand

701 – 705

5

Helix

707 – 717

11

Helix

722 – 724

3

Helix

725 – 735

11

Sequences

	Sequence		Length	Mass (Da)
	P18708-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: F3E0CEB2CF1BD582	FASTA	744	82,536
	10 20 30 40 50 60 MAGRSMQAAR CPTDELSLSN CAVVSEKDYQ SGQHVIVRTS PNHKYIFTLR THPSVVPGSV 70 80 90 100 110 120 AFSLPQRKWA GLSIGQEIEV ALYSFDKAKQ CIGTMTIEID FLQKKNIDSN PYDTDKMAAE 130 140 150 160 170 180 FIQQFNNQAF SVGQQLVFSF NDKLFGLLVK DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN 190 200 210 220 230 240 SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF 250 260 270 280 290 300 PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 310 320 330 340 350 360 NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG 370 380 390 400 410 420 VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL 430 440 450 460 470 480 SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF 490 500 510 520 530 540 LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL 550 560 570 580 590 600 LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 610 620 630 640 650 660 VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA 670 680 690 700 710 720 FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM 730 740 DPEYRVRKFL ALLREEGASP LDFD

References

[1]	"A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast." Wilson D.W., Wilcox C.A., Flynn G.C., Chen E., Kuang W.-J., Henzel W.J., Block M.R., Ullrich A., Rothman J.E. Nature 339:355-359(1989) [PubMed: 2657434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Ovary.
[2]	"Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein." Beckers C.J.M., Block M.R., Glick B.S., Rothman J.E., Balch W.E. Nature 339:397-398(1989) [PubMed: 2542798] [Abstract] Cited for: FUNCTION.
[3]	"Crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein." Lenzen C.U., Steinmann D., Whiteheart S.W., Weis W.I. Cell 94:525-536(1998) [PubMed: 9727495] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 497-742.
[4]	Erratum Lenzen C.U., Steinmann D., Whiteheart S.W., Weis W.I. Cell 95:289-289(1998)
[5]	"Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP." Yu R.C., Hanson P.I., Jahn R., Bruenger A.T. Nat. Struct. Biol. 5:803-811(1998) [PubMed: 9731775] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 489-735.
[6]	"NSF N-terminal domain crystal structure: models of NSF function." Yu R.C., Jahn R., Brunger A.T. Mol. Cell 4:97-107(1999) [PubMed: 10445031] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-205.
[7]	"Crystal structure of the amino-terminal domain of N-ethylmaleimide-sensitive fusion protein." May A.P., Misura K.M., Whiteheart S.W., Weis W.I. Nat. Cell Biol. 1:175-182(1999) [PubMed: 10559905] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-203.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X15652 mRNA. Translation: CAA33678.1. Different initiation.

PIR

S04235.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D2N	X-ray	1.75	A	487-743	[»]
1NSF	X-ray	1.90	A	478-744	[»]
1QCS	X-ray	1.90	A	1-205	[»]
1QDN	X-ray	2.30	A/B/C	1-203	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P18708. 3 interactions.

Phylogenomic databases

HOVERGEN

P18708.

Enzyme and pathway databases

BRENDA

3.6.4.6. 18.

Family and domain databases

InterPro

IPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR003338. ATPase_AAA_VAT_N.
IPR004201. Cell_division_protein_CDC48_2.
IPR001984. Peptidase_S16_C.
[Graphical view]

Pfam

PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]

PRINTS

PR00830. ENDOLAPTASE.

SMART

SM00382. AAA. 2 hits.
[Graphical view]

PROSITE

PS00674. AAA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NSF_CRIGR

Accession

Primary (citable) accession number: P18708

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)