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P18708

- NSF_CRIGR

UniProt

P18708 - NSF_CRIGR

Protein

Vesicle-fusing ATPase

Gene

NSF

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi550 – 5501Magnesium

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi505 – 5106ATP
    Nucleotide bindingi545 – 5528ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. nucleoside-triphosphatase activity Source: InterPro

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-fusing ATPase (EC:3.6.4.6)
    Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein
    Short name:
    NEM-sensitive fusion protein
    Vesicular-fusion protein NSF
    Gene namesi
    Name:NSF
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. midbody Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744Vesicle-fusing ATPasePRO_0000084562Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei105 – 1051N6-acetyllysineBy similarity
    Modified residuei259 – 2591PhosphotyrosineBy similarity
    Modified residuei569 – 5691Phosphoserine; by CDK16By similarity

    Post-translational modificationi

    Phosphorylation at Ser-569 interferes with homohexamerization.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP18708.

    Interactioni

    Subunit structurei

    Homohexamer. Interacts with GABARAP and GABARAPL2 By similarity. Interacts with GRIA2 By similarity. Interacts with PLK2, leading to disrupt the interaction with GRIA2 By similarity. Interacts with MUSK; may regulate MUSK endocytosis and activity By similarity. Interacts with CDK16 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-35598N.
    IntActiP18708. 3 interactions.

    Structurei

    Secondary structure

    1
    744
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 107
    Helixi14 – 185
    Beta strandi22 – 243
    Turni26 – 283
    Beta strandi34 – 407
    Beta strandi43 – 519
    Beta strandi59 – 624
    Helixi64 – 707
    Beta strandi77 – 837
    Helixi87 – 904
    Beta strandi91 – 10212
    Helixi104 – 1063
    Beta strandi111 – 1133
    Helixi114 – 12512
    Beta strandi129 – 1313
    Beta strandi135 – 1406
    Beta strandi143 – 15412
    Beta strandi173 – 1764
    Beta strandi182 – 1876
    Beta strandi194 – 2007
    Turni500 – 5023
    Helixi512 – 53019
    Beta strandi535 – 5428
    Helixi549 – 56012
    Beta strandi563 – 5686
    Helixi570 – 5723
    Helixi578 – 59316
    Beta strandi595 – 6028
    Helixi605 – 6084
    Turni613 – 6164
    Helixi620 – 62910
    Beta strandi639 – 6479
    Helixi649 – 6546
    Turni658 – 6603
    Beta strandi661 – 6666
    Beta strandi670 – 6723
    Helixi673 – 68311
    Helixi688 – 69811
    Beta strandi701 – 7055
    Helixi707 – 71711
    Helixi722 – 7243
    Helixi725 – 73511

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1D2NX-ray1.75A487-740[»]
    1NSFX-ray1.90A478-744[»]
    1QCSX-ray1.90A1-205[»]
    1QDNX-ray2.30A/B/C1-203[»]
    ProteinModelPortaliP18708.
    SMRiP18708. Positions 1-201, 489-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18708.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG000324.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18708-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRSMQAAR CPTDELSLSN CAVVSEKDYQ SGQHVIVRTS PNHKYIFTLR    50
    THPSVVPGSV AFSLPQRKWA GLSIGQEIEV ALYSFDKAKQ CIGTMTIEID 100
    FLQKKNIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NDKLFGLLVK 150
    DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK 200
    TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC 250
    KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 300
    NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV 350
    VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE 400
    KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS 450
    TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA 500
    SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT 550
    ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 600
    VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD 650
    VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG 700
    KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALLREEGASP LDFD 744
    Length:744
    Mass (Da):82,536
    Last modified:November 1, 1990 - v1
    Checksum:iF3E0CEB2CF1BD582
    GO

    Sequence cautioni

    The sequence CAA33678.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15652 mRNA. Translation: CAA33678.1. Different initiation.
    PIRiS04235.
    RefSeqiXP_007606206.1. XM_007608016.1.

    Genome annotation databases

    GeneIDi100770898.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15652 mRNA. Translation: CAA33678.1 . Different initiation.
    PIRi S04235.
    RefSeqi XP_007606206.1. XM_007608016.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1D2N X-ray 1.75 A 487-740 [» ]
    1NSF X-ray 1.90 A 478-744 [» ]
    1QCS X-ray 1.90 A 1-205 [» ]
    1QDN X-ray 2.30 A/B/C 1-203 [» ]
    ProteinModelPortali P18708.
    SMRi P18708. Positions 1-201, 489-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35598N.
    IntActi P18708. 3 interactions.

    Proteomic databases

    PRIDEi P18708.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100770898.

    Phylogenomic databases

    HOVERGENi HBG000324.

    Miscellaneous databases

    EvolutionaryTracei P18708.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast."
      Wilson D.W., Wilcox C.A., Flynn G.C., Chen E., Kuang W.-J., Henzel W.J., Block M.R., Ullrich A., Rothman J.E.
      Nature 339:355-359(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Ovary.
    2. "Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein."
      Beckers C.J.M., Block M.R., Glick B.S., Rothman J.E., Balch W.E.
      Nature 339:397-398(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "Crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein."
      Lenzen C.U., Steinmann D., Whiteheart S.W., Weis W.I.
      Cell 94:525-536(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 497-742 IN COMPLEX WITH ATP ANALOG.
    4. Erratum
      Lenzen C.U., Steinmann D., Whiteheart S.W., Weis W.I.
      Cell 95:289-289(1998)
    5. "Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP."
      Yu R.C., Hanson P.I., Jahn R., Bruenger A.T.
      Nat. Struct. Biol. 5:803-811(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 489-735.
    6. "NSF N-terminal domain crystal structure: models of NSF function."
      Yu R.C., Jahn R., Brunger A.T.
      Mol. Cell 4:97-107(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-205.
    7. "Crystal structure of the amino-terminal domain of N-ethylmaleimide-sensitive fusion protein."
      May A.P., Misura K.M., Whiteheart S.W., Weis W.I.
      Nat. Cell Biol. 1:175-182(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-203.

    Entry informationi

    Entry nameiNSF_CRIGR
    AccessioniPrimary (citable) accession number: P18708
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3