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Protein

Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Gene

PHKA1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Enzyme regulationi

By phosphorylation of various serine residues and by calcium.

Pathwayi: glycogen metabolism

This protein is involved in the pathway glycogen metabolism, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen metabolism and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

BRENDAi2.7.11.19. 1749.
UniPathwayiUPA00163.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Short name:
Phosphorylase kinase alpha M subunit
Gene namesi
Name:PHKA1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12371237Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoformPRO_0000057729Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei629 – 6291PhosphoserineBy similarity
Modified residuei729 – 7291PhosphoserineBy similarity
Modified residuei735 – 7351PhosphoserineBy similarity
Modified residuei758 – 7581PhosphoserineBy similarity
Modified residuei972 – 9721Phosphoserine; by autocatalysis2 Publications
Modified residuei981 – 9811PhosphoserineBy similarity
Modified residuei985 – 9851Phosphoserine; by autocatalysis2 Publications
Modified residuei1007 – 10071Phosphoserine; by autocatalysis2 Publications
Modified residuei1018 – 10181Phosphoserine; by PKA3 Publications
Modified residuei1020 – 10201Phosphoserine2 Publications
Modified residuei1023 – 10231Phosphoserine2 Publications
Modified residuei1030 – 10301Phosphoserine2 Publications
Modified residuei1127 – 11271PhosphoserineBy similarity
Lipidationi1234 – 12341S-farnesyl cysteine1 Publication

Post-translational modificationi

Phosphorylation of Ser-1018 by PKA stimulates the dephosphorylation of the beta subunit and, thus, reverses the initial stimulation of PHK by the faster beta-subunit phosphorylation by PKA, that occurs in muscle in response to adrenaline.2 Publications
Cys-1234 is farnesylated, but the C-terminal tripeptide is not removed and the cysteine carboxyl is not methylated.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1234 – 12341Not methylated1 Publication

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

PTM databases

iPTMnetiP18688.

Expressioni

Tissue specificityi

Isoform 1 predominates in muscle, heart, brain and testis. Isoforms 1 and 2 are expressed in similar quantities in the other tissues. Isoform 3 is highly expressed in slow muscle and heart.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Protein-protein interaction databases

DIPiDIP-44274N.
MINTiMINT-5209269.
STRINGi9986.ENSOCUP00000024040.

Structurei

3D structure databases

ProteinModelPortaliP18688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni810 – 84031Calmodulin-bindingSequence analysisAdd
BLAST
Regioni1060 – 110041Calmodulin-bindingSequence analysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3635. Eukaryota.
ENOG410XPJZ. LUCA.
HOGENOMiHOG000231478.
HOVERGENiHBG000273.
InParanoidiP18688.
KOiK07190.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011613. Glyco_hydro_15/PHK.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERiPTHR10749. PTHR10749. 2 hits.
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P18688-1) [UniParc]FASTAAdd to basket
Also known as: ABC, Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSRSNSGVR LDSYARLVQQ TILCHQNPVT GLLPASYDQK DAWVRDNVYS
60 70 80 90 100
ILAVWGLGLA YRKNADRDED KAKAYELEQS VVKLMRGLLH CMIRQVDKVE
110 120 130 140 150
SFKYSQSTKD SLHAKYNTKT CATVVGDDQW GHLQLDATSV YLLFLAQMTA
160 170 180 190 200
SGLHIIHSLD EVNFIQNLVF YIEAAYKTAD FGIWERGDKT NQGISELNAS
210 220 230 240 250
SVGMAKAALE ALDELDLFGV KGGPQSVIHV LADEVQHCQS ILNSLLPRAS
260 270 280 290 300
TSKEVDASLL SVISFPAFAV EDSKLVEITK QEIITKLQGR YGCCRFLRDG
310 320 330 340 350
YKTPKEDPNR LYYEPAELKL FENIECEWPL FWTYFILDGV FSGNAEQVQE
360 370 380 390 400
YREALEAVLI KGKNGVPLLP ELYSVPPDKV DEEYQNPHTV DRVPMGKLPH
410 420 430 440 450
MWGQSLYILG SLMAEGFLAP GEIDPLNRRF STVPKPDVVV QVSILAETEE
460 470 480 490 500
IKAILKDKGI NVETIAEVYP IRVQPARILS HIYSSLGCNN RMKLSGRPYR
510 520 530 540 550
HMGVLGTSKL YDIRKTIFTF TPQFIDQQQF YLALDNKMIV EMLRTDLSYL
560 570 580 590 600
CSRWRMTGQP TITFPISQTM LDEDGTSLNS SILAALRKMQ DGYFGGARIQ
610 620 630 640 650
TGKLSEFLTT SCCTHLSFMD PGPEGKLYSE DYDDNYDELE SGDWMDGYNS
660 670 680 690 700
TSTARCGDEV ARYLDHLLAH TAPHPKLAPA SQKGGLNRFR AAVQTTCDLM
710 720 730 740 750
SLVTKAKELH VQNVHMYLPT KLFQASRPSL NLLDSSHPSQ EDQVPTVRVE
760 770 780 790 800
VHLPRDQSGE VDFQALVLQL KETSSLQEQA DILYMLYTMK GPDWDTELYE
810 820 830 840 850
EGSATVRELL TELYGKVGKI RHWGLIRYIS GILRKKVEAL DEACTDLLSH
860 870 880 890 900
QKHLTVGLPP EPREKTISAP LPYEALTRLI EEACEGDMNI SILTQEIMVY
910 920 930 940 950
LAMYMRTQPG LFAEMFRLRI GLIIQVMATE LAHSLRCSAE EATEGLMNLS
960 970 980 990 1000
PSAMKNLLHH ILSGKEFGVE RSVRPTDSNV SPAISIHEIG AVGATKTERT
1010 1020 1030 1040 1050
GIMQLKSEIK QVEFRRLSIS TESQPNGGHS LGADLMSPSF LSPGTSVTPS
1060 1070 1080 1090 1100
SGSFPGHHTS KDSRQGQWQR RRRLDGALNR VPIGFYQKVW KVLQKCHGLS
1110 1120 1130 1140 1150
VEGFVLPSST TREMTPGEIK FSVHVESVLN RVPQPEYRQL LVEAILVLTM
1160 1170 1180 1190 1200
LADIEIHSIG SIIAVEKIVH IANDLFLQEQ KTLGADDIML AKDPASGICT
1210 1220 1230
LLYDSAPSGR FGTMTYLSKA AATYVQEFLP HSICAMQ
Length:1,237
Mass (Da):138,413
Last modified:November 1, 1990 - v1
Checksum:i9B53EBA29D4B33FF
GO
Isoform 2 (identifier: P18688-2) [UniParc]FASTAAdd to basket
Also known as: BC

The sequence of this isoform differs from the canonical sequence as follows:
     1012-1024: Missing.

Show »
Length:1,224
Mass (Da):136,880
Checksum:i65024910AF8BFAF0
GO
Isoform 3 (identifier: P18688-3) [UniParc]FASTAAdd to basket
Also known as: Alpha'

The sequence of this isoform differs from the canonical sequence as follows:
     654-712: Missing.
     713-713: N → D

Show »
Length:1,178
Mass (Da):131,996
Checksum:i7D5DDCA1107A1CA1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei654 – 71259Missing in isoform 3. 1 PublicationVSP_004699Add
BLAST
Alternative sequencei713 – 7131N → D in isoform 3. 1 PublicationVSP_004700
Alternative sequencei1012 – 102413Missing in isoform 2. CuratedVSP_004701Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03247 mRNA. Translation: AAA31446.1. Sequence problems.
M64656 mRNA. Translation: AAC23909.1.
PIRiA31334.
RefSeqiNP_001159389.1. NM_001165917.1. [P18688-3]
UniGeneiOcu.2209.

Genome annotation databases

GeneIDi100303771.
KEGGiocu:100303771.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03247 mRNA. Translation: AAA31446.1. Sequence problems.
M64656 mRNA. Translation: AAC23909.1.
PIRiA31334.
RefSeqiNP_001159389.1. NM_001165917.1. [P18688-3]
UniGeneiOcu.2209.

3D structure databases

ProteinModelPortaliP18688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-44274N.
MINTiMINT-5209269.
STRINGi9986.ENSOCUP00000024040.

PTM databases

iPTMnetiP18688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100303771.
KEGGiocu:100303771.

Organism-specific databases

CTDi5255.

Phylogenomic databases

eggNOGiKOG3635. Eukaryota.
ENOG410XPJZ. LUCA.
HOGENOMiHOG000231478.
HOVERGENiHBG000273.
InParanoidiP18688.
KOiK07190.

Enzyme and pathway databases

UniPathwayiUPA00163.
BRENDAi2.7.11.19. 1749.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011613. Glyco_hydro_15/PHK.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERiPTHR10749. PTHR10749. 2 hits.
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKPB1_RABIT
AccessioniPrimary (citable) accession number: P18688
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: July 6, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.