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Protein

Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Gene

PHKA1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Enzyme regulationi

By phosphorylation of various serine residues and by calcium.

Pathwayi: glycogen metabolism

This protein is involved in the pathway glycogen metabolism, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen metabolism and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Muscle protein
Biological processCarbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BRENDAi2.7.11.19 1749
UniPathwayiUPA00163

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Short name:
Phosphorylase kinase alpha M subunit
Gene namesi
Name:PHKA1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577291 – 1237Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoformAdd BLAST1237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei629PhosphoserineBy similarity1
Modified residuei729PhosphoserineBy similarity1
Modified residuei735PhosphoserineBy similarity1
Modified residuei758PhosphoserineBy similarity1
Modified residuei972Phosphoserine; by autocatalysis2 Publications1
Modified residuei981PhosphoserineBy similarity1
Modified residuei985Phosphoserine; by autocatalysis2 Publications1
Modified residuei1007Phosphoserine; by autocatalysis2 Publications1
Modified residuei1018Phosphoserine; by PKA3 Publications1
Modified residuei1020Phosphoserine2 Publications1
Modified residuei1023Phosphoserine2 Publications1
Modified residuei1030Phosphoserine2 Publications1
Modified residuei1127PhosphoserineBy similarity1
Lipidationi1234S-farnesyl cysteine1 Publication1

Post-translational modificationi

Phosphorylation of Ser-1018 by PKA stimulates the dephosphorylation of the beta subunit and, thus, reverses the initial stimulation of PHK by the faster beta-subunit phosphorylation by PKA, that occurs in muscle in response to adrenaline.2 Publications
Cys-1234 is farnesylated, but the C-terminal tripeptide is not removed and the cysteine carboxyl is not methylated.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1234Not methylated1 Publication1

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PRIDEiP18688

PTM databases

iPTMnetiP18688

Expressioni

Tissue specificityi

Isoform 1 predominates in muscle, heart, brain and testis. Isoforms 1 and 2 are expressed in similar quantities in the other tissues. Isoform 3 is highly expressed in slow muscle and heart.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-44274N
IntActiP18688, 1 interactor
MINTiP18688
STRINGi9986.ENSOCUP00000024040

Chemistry databases

BindingDBiP18688

Structurei

3D structure databases

ProteinModelPortaliP18688
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni810 – 840Calmodulin-bindingSequence analysisAdd BLAST31
Regioni1060 – 1100Calmodulin-bindingSequence analysisAdd BLAST41

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3635 Eukaryota
ENOG410XPJZ LUCA
HOGENOMiHOG000231478
HOVERGENiHBG000273
InParanoidiP18688
KOiK07190

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR011613 Glyco_hydro_15/PHK
IPR008734 PHK_A/B_su
PANTHERiPTHR10749 PTHR10749, 1 hit
PfamiView protein in Pfam
PF00723 Glyco_hydro_15, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P18688-1) [UniParc]FASTAAdd to basket
Also known as: ABC, Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSRSNSGVR LDSYARLVQQ TILCHQNPVT GLLPASYDQK DAWVRDNVYS
60 70 80 90 100
ILAVWGLGLA YRKNADRDED KAKAYELEQS VVKLMRGLLH CMIRQVDKVE
110 120 130 140 150
SFKYSQSTKD SLHAKYNTKT CATVVGDDQW GHLQLDATSV YLLFLAQMTA
160 170 180 190 200
SGLHIIHSLD EVNFIQNLVF YIEAAYKTAD FGIWERGDKT NQGISELNAS
210 220 230 240 250
SVGMAKAALE ALDELDLFGV KGGPQSVIHV LADEVQHCQS ILNSLLPRAS
260 270 280 290 300
TSKEVDASLL SVISFPAFAV EDSKLVEITK QEIITKLQGR YGCCRFLRDG
310 320 330 340 350
YKTPKEDPNR LYYEPAELKL FENIECEWPL FWTYFILDGV FSGNAEQVQE
360 370 380 390 400
YREALEAVLI KGKNGVPLLP ELYSVPPDKV DEEYQNPHTV DRVPMGKLPH
410 420 430 440 450
MWGQSLYILG SLMAEGFLAP GEIDPLNRRF STVPKPDVVV QVSILAETEE
460 470 480 490 500
IKAILKDKGI NVETIAEVYP IRVQPARILS HIYSSLGCNN RMKLSGRPYR
510 520 530 540 550
HMGVLGTSKL YDIRKTIFTF TPQFIDQQQF YLALDNKMIV EMLRTDLSYL
560 570 580 590 600
CSRWRMTGQP TITFPISQTM LDEDGTSLNS SILAALRKMQ DGYFGGARIQ
610 620 630 640 650
TGKLSEFLTT SCCTHLSFMD PGPEGKLYSE DYDDNYDELE SGDWMDGYNS
660 670 680 690 700
TSTARCGDEV ARYLDHLLAH TAPHPKLAPA SQKGGLNRFR AAVQTTCDLM
710 720 730 740 750
SLVTKAKELH VQNVHMYLPT KLFQASRPSL NLLDSSHPSQ EDQVPTVRVE
760 770 780 790 800
VHLPRDQSGE VDFQALVLQL KETSSLQEQA DILYMLYTMK GPDWDTELYE
810 820 830 840 850
EGSATVRELL TELYGKVGKI RHWGLIRYIS GILRKKVEAL DEACTDLLSH
860 870 880 890 900
QKHLTVGLPP EPREKTISAP LPYEALTRLI EEACEGDMNI SILTQEIMVY
910 920 930 940 950
LAMYMRTQPG LFAEMFRLRI GLIIQVMATE LAHSLRCSAE EATEGLMNLS
960 970 980 990 1000
PSAMKNLLHH ILSGKEFGVE RSVRPTDSNV SPAISIHEIG AVGATKTERT
1010 1020 1030 1040 1050
GIMQLKSEIK QVEFRRLSIS TESQPNGGHS LGADLMSPSF LSPGTSVTPS
1060 1070 1080 1090 1100
SGSFPGHHTS KDSRQGQWQR RRRLDGALNR VPIGFYQKVW KVLQKCHGLS
1110 1120 1130 1140 1150
VEGFVLPSST TREMTPGEIK FSVHVESVLN RVPQPEYRQL LVEAILVLTM
1160 1170 1180 1190 1200
LADIEIHSIG SIIAVEKIVH IANDLFLQEQ KTLGADDIML AKDPASGICT
1210 1220 1230
LLYDSAPSGR FGTMTYLSKA AATYVQEFLP HSICAMQ
Length:1,237
Mass (Da):138,413
Last modified:November 1, 1990 - v1
Checksum:i9B53EBA29D4B33FF
GO
Isoform 2 (identifier: P18688-2) [UniParc]FASTAAdd to basket
Also known as: BC

The sequence of this isoform differs from the canonical sequence as follows:
     1012-1024: Missing.

Show »
Length:1,224
Mass (Da):136,880
Checksum:i65024910AF8BFAF0
GO
Isoform 3 (identifier: P18688-3) [UniParc]FASTAAdd to basket
Also known as: Alpha'

The sequence of this isoform differs from the canonical sequence as follows:
     654-712: Missing.
     713-713: N → D

Show »
Length:1,178
Mass (Da):131,996
Checksum:i7D5DDCA1107A1CA1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004699654 – 712Missing in isoform 3. 1 PublicationAdd BLAST59
Alternative sequenceiVSP_004700713N → D in isoform 3. 1 Publication1
Alternative sequenceiVSP_0047011012 – 1024Missing in isoform 2. CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03247 mRNA Translation: AAA31446.1 Sequence problems.
M64656 mRNA Translation: AAC23909.1
PIRiA31334
RefSeqiNP_001159389.1, NM_001165917.1 [P18688-3]
UniGeneiOcu.2209

Genome annotation databases

GeneIDi100303771
KEGGiocu:100303771

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKPB1_RABIT
AccessioniPrimary (citable) accession number: P18688
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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