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P18670

- LECA_ARTIN

UniProt

P18670 - LECA_ARTIN

Protein

Agglutinin alpha chain

Gene
N/A
Organism
Artocarpus integer (Jack fruit) (Artocarpus integrifolia)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.

    GO - Molecular functioni

    1. carbohydrate binding Source: UniProtKB
    2. IgA binding Source: UniProtKB

    Keywords - Ligandi

    IgA-binding protein, Lectin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Agglutinin alpha chain
    Alternative name(s):
    Jacalin alpha chain
    OrganismiArtocarpus integer (Jack fruit) (Artocarpus integrifolia)
    Taxonomic identifieri3490 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesMoraceaeArtocarpus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 133133Agglutinin alpha chainPRO_0000072798Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi43 – 431N-linked (GlcNAc...); when associated with variant T-45; partial1 Publication
    Glycosylationi74 – 741N-linked (GlcNAc...); partial1 Publication

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Tetramer of four alpha chains associated with two or four beta chains.1 Publication

    Structurei

    Secondary structure

    1
    133
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 54
    Beta strandi10 – 1910
    Beta strandi21 – 233
    Beta strandi25 – 3410
    Beta strandi37 – 404
    Beta strandi52 – 576
    Turni60 – 623
    Beta strandi65 – 7511
    Beta strandi78 – 9013
    Beta strandi92 – 976
    Beta strandi101 – 1077
    Beta strandi112 – 13221

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JACX-ray2.43A/C/E/G1-133[»]
    1KU8X-ray1.75A/C/E/G1-133[»]
    1KUJX-ray2.00A/C/E/G1-133[»]
    1M26X-ray1.62A/C/E/G1-133[»]
    1PXDX-ray1.80A1-133[»]
    1TOQX-ray2.50A/C/E/G1-133[»]
    1TP8X-ray3.00A/C/E/G1-133[»]
    1UGWX-ray1.70A/C/E/G1-133[»]
    1UGXX-ray1.60A1-133[»]
    1UGYX-ray2.40A/C/E/G1-133[»]
    1UH0X-ray2.80A/C/E/G1-133[»]
    1UH1X-ray2.80A/C/E/G1-133[»]
    1WS4X-ray1.90A/C/E/G1-133[»]
    1WS5X-ray1.90A/C/E/G1-133[»]
    4AK4X-ray1.65A/C/E/G/I/K/M/O1-133[»]
    4AKBX-ray1.95A/C/E/G1-133[»]
    4AKCX-ray2.30A/C/E/G1-133[»]
    ProteinModelPortaliP18670.
    SMRiP18670. Positions 1-133.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18670.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati7 – 6458Add
    BLAST
    Repeati76 – 13055Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni68 – 8922IgA-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the jacalin lectin family.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di2.100.10.30. 1 hit.
    InterProiIPR001229. Mannose-bd_lectin.
    [Graphical view]
    PfamiPF01419. Jacalin. 1 hit.
    [Graphical view]
    SMARTiSM00915. Jacalin. 1 hit.
    [Graphical view]
    SUPFAMiSSF51101. SSF51101. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18670-1 [UniParc]FASTAAdd to Basket

    « Hide

    GKAFDDGAFT GIREINLSYN KETAIGDFQV VYDLNGSPYV GQNHKSFITG    50
    FTPVKISLDF PSEYIMEVSG YTGNVSGYVV VRSLTFKTNK KTYGPYGVTS 100
    GTPFNLPIEN GLIVGFKGSI GYWLDYFSMY LSL 133
    Length:133
    Mass (Da):14,663
    Last modified:August 1, 1991 - v2
    Checksum:iFF10513379CB2E10
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191Y → I AA sequence (PubMed:8427879)Curated
    Sequence conflicti75 – 751V → Y AA sequence (PubMed:1599414)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311V → I.1 Publication
    Natural varianti34 – 341L → G.
    Natural varianti45 – 451K → L.
    Natural varianti45 – 451K → T.1 Publication
    Natural varianti66 – 661M → D.
    Natural varianti66 – 661M → V.1 Publication
    Natural varianti67 – 671E → M.
    Natural varianti72 – 721T → I.
    Natural varianti74 – 741N → K.
    Natural varianti102 – 1021T → D.
    Natural varianti113 – 1131I → V.
    Natural varianti131 – 1311L → N.

    Sequence databases

    PIRiS29641.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    PIRi S29641.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JAC X-ray 2.43 A/C/E/G 1-133 [» ]
    1KU8 X-ray 1.75 A/C/E/G 1-133 [» ]
    1KUJ X-ray 2.00 A/C/E/G 1-133 [» ]
    1M26 X-ray 1.62 A/C/E/G 1-133 [» ]
    1PXD X-ray 1.80 A 1-133 [» ]
    1TOQ X-ray 2.50 A/C/E/G 1-133 [» ]
    1TP8 X-ray 3.00 A/C/E/G 1-133 [» ]
    1UGW X-ray 1.70 A/C/E/G 1-133 [» ]
    1UGX X-ray 1.60 A 1-133 [» ]
    1UGY X-ray 2.40 A/C/E/G 1-133 [» ]
    1UH0 X-ray 2.80 A/C/E/G 1-133 [» ]
    1UH1 X-ray 2.80 A/C/E/G 1-133 [» ]
    1WS4 X-ray 1.90 A/C/E/G 1-133 [» ]
    1WS5 X-ray 1.90 A/C/E/G 1-133 [» ]
    4AK4 X-ray 1.65 A/C/E/G/I/K/M/O 1-133 [» ]
    4AKB X-ray 1.95 A/C/E/G 1-133 [» ]
    4AKC X-ray 2.30 A/C/E/G 1-133 [» ]
    ProteinModelPortali P18670.
    SMRi P18670. Positions 1-133.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P18670.

    Family and domain databases

    Gene3Di 2.100.10.30. 1 hit.
    InterProi IPR001229. Mannose-bd_lectin.
    [Graphical view ]
    Pfami PF01419. Jacalin. 1 hit.
    [Graphical view ]
    SMARTi SM00915. Jacalin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51101. SSF51101. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The amino acid sequences of jacalin and the Maclura pomifera agglutinin."
      Young N.M., Johnston R.A.Z., Watson D.C.
      FEBS Lett. 282:382-384(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Seed.
    2. "Primary structure of a Thomsen-Friedenreich-antigen-specific lectin, jacalin [Artocarpus integrifolia (jack fruit) agglutinin]. Evidence for the presence of an internal repeat."
      Mahanta S.K., Sanker S., Prasad Rao N.V.S.A.V., Swamy M.J., Surolia A.
      Biochem. J. 284:95-101(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Fruit.
    3. "Structural and electron-microscopic studies of jacalin from jackfruit (Artocarpus integrifolia) show that this lectin is a 65 kDa tetramer."
      Ruffet E., Paquet N., Frutiger S., Hughes G.J., Jaton J.-C.
      Biochem. J. 286:131-134(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, VARIANTS ILE-31; THR-45 AND VAL-66, GLYCOSYLATION AT ASN-43 AND ASN-74, SUBUNIT.
      Tissue: Seed.
    4. "Homology of the D-galactose-specific lectins from Artocarpus integrifolia and Maclura pomifera and the role of an unusual small polypeptide subunit."
      Young N.M., Johnston R.A.Z., Szabo A.G., Watson D.C.
      Arch. Biochem. Biophys. 270:596-603(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-33.
      Tissue: Seed.
    5. "Identification of a novel 4 kDa immunoglobulin-A-binding peptide obtained by the limited proteolysis of jacalin."
      Kabir S., Aebersold R., Daar A.S.
      Biochim. Biophys. Acta 1161:194-200(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-29 AND 68-89.
      Tissue: Seed.
    6. "The alpha- and beta-subunits of the jacalins are cleavage products from a 17-kDa precursor."
      Ngoc L.D., Brillard M., Hoebeke J.
      Biochim. Biophys. Acta 1156:219-222(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-27.
    7. "A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold."
      Sakaranarayanan R., Sekar S., Banerjee R., Sharma V., Surolia A., Vijayan M.
      Nat. Struct. Biol. 3:596-603(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.43 ANGSTROMS).

    Entry informationi

    Entry nameiLECA_ARTIN
    AccessioniPrimary (citable) accession number: P18670
    Secondary accession number(s): P80023, Q9S788
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3