P18670 (LECA_ARTIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Agglutinin alpha chain Alternative name(s): Jacalin alpha chain
Organism	Artocarpus integer (Jack fruit) (Artocarpus integrifolia)
Taxonomic identifier	3490 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Moraceae › Artocarpus

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.
Subunit structure	Tetramer of four alpha chains associated with two or four beta chains. Ref.3
Sequence similarities	Belongs to the jacalin lectin family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	IgA-binding protein Lectin
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	IgA binding Inferred from direct assay Ref.5. Source: UniProtKB carbohydrate binding Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 133

133

Agglutinin alpha chain

PRO_0000072798

Regions

Repeat

7 – 64

58

Repeat

76 – 130

55

Region

68 – 89

22

IgA-binding

Amino acid modifications

Glycosylation

43

1

N-linked (GlcNAc...); when associated with variant T-45; partial Ref.3

Glycosylation

74

1

N-linked (GlcNAc...); partial Ref.3

Natural variations

Natural variant

31

1

V → I. Ref.3

Natural variant

34

1

L → G.

Natural variant

45

1

K → L.

Natural variant

45

1

K → T. Ref.3

Natural variant

66

1

M → D.

Natural variant

66

1

M → V. Ref.3

Natural variant

67

1

E → M.

Natural variant

72

1

T → I.

Natural variant

74

1

N → K.

Natural variant

102

1

T → D.

Natural variant

113

1

I → V.

Natural variant

131

1

L → N.

Experimental info

Sequence conflict

19

1

Y → I AA sequence Ref.6

Sequence conflict

75

1

V → Y AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

.

.

.

.

.

133

Helix Strand Turn

Details...

Beta strand

2 – 5

4

Beta strand

10 – 19

10

Beta strand

21 – 23

3

Beta strand

25 – 34

10

Beta strand

37 – 40

4

Beta strand

52 – 57

6

Turn

60 – 62

3

Beta strand

65 – 75

11

Beta strand

78 – 90

13

Beta strand

92 – 97

6

Beta strand

101 – 107

7

Beta strand

112 – 132

21

Sequences

	Sequence		Length	Mass (Da)
	P18670 [UniParc]. Last modified August 1, 1991. Version 2. Checksum: FF10513379CB2E10	FASTA	133	14,663
	10 20 30 40 50 60 GKAFDDGAFT GIREINLSYN KETAIGDFQV VYDLNGSPYV GQNHKSFITG FTPVKISLDF 70 80 90 100 110 120 PSEYIMEVSG YTGNVSGYVV VRSLTFKTNK KTYGPYGVTS GTPFNLPIEN GLIVGFKGSI 130 GYWLDYFSMY LSL

References

[1]	"The amino acid sequences of jacalin and the Maclura pomifera agglutinin." Young N.M., Johnston R.A.Z., Watson D.C. FEBS Lett. 282:382-384(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed.
[2]	"Primary structure of a Thomsen-Friedenreich-antigen-specific lectin, jacalin [Artocarpus integrifolia (jack fruit) agglutinin]. Evidence for the presence of an internal repeat." Mahanta S.K., Sanker S., Prasad Rao N.V.S.A.V., Swamy M.J., Surolia A. Biochem. J. 284:95-101(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Fruit.
[3]	"Structural and electron-microscopic studies of jacalin from jackfruit (Artocarpus integrifolia) show that this lectin is a 65 kDa tetramer." Ruffet E., Paquet N., Frutiger S., Hughes G.J., Jaton J.-C. Biochem. J. 286:131-134(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, VARIANTS ILE-31; THR-45 AND VAL-66, GLYCOSYLATION AT ASN-43 AND ASN-74, SUBUNIT. Tissue: Seed.
[4]	"Homology of the D-galactose-specific lectins from Artocarpus integrifolia and Maclura pomifera and the role of an unusual small polypeptide subunit." Young N.M., Johnston R.A.Z., Szabo A.G., Watson D.C. Arch. Biochem. Biophys. 270:596-603(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-33. Tissue: Seed.
[5]	"Identification of a novel 4 kDa immunoglobulin-A-binding peptide obtained by the limited proteolysis of jacalin." Kabir S., Aebersold R., Daar A.S. Biochim. Biophys. Acta 1161:194-200(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-29 AND 68-89. Tissue: Seed.
[6]	"The alpha- and beta-subunits of the jacalins are cleavage products from a 17-kDa precursor." Ngoc L.D., Brillard M., Hoebeke J. Biochim. Biophys. Acta 1156:219-222(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-27.
[7]	"A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold." Sakaranarayanan R., Sekar S., Banerjee R., Sharma V., Surolia A., Vijayan M. Nat. Struct. Biol. 3:596-603(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.43 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S29641.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JAC	X-ray	2.43	A/C/E/G	1-133	[»]
1KU8	X-ray	1.75	A/C/E/G	1-133	[»]
1KUJ	X-ray	2.00	A/C/E/G	1-133	[»]
1M26	X-ray	1.62	A/C/E/G	1-133	[»]
1PXD	X-ray	1.80	A	1-133	[»]
1TOQ	X-ray	2.50	A/C/E/G	1-133	[»]
1TP8	X-ray	3.00	A/C/E/G	1-133	[»]
1UGW	X-ray	1.70	A/C/E/G	1-133	[»]
1UGX	X-ray	1.60	A	1-133	[»]
1UGY	X-ray	2.40	A/C/E/G	1-133	[»]
1UH0	X-ray	2.80	A/C/E/G	1-133	[»]
1UH1	X-ray	2.80	A/C/E/G	1-133	[»]
1WS4	X-ray	1.90	A/C/E/G	1-133	[»]
1WS5	X-ray	1.90	A/C/E/G	1-133	[»]
4AK4	X-ray	1.65	A/C/E/G/I/K/M/O	1-133	[»]
4AKB	X-ray	1.95	A/C/E/G	1-133	[»]
4AKC	X-ray	2.30	A/C/E/G	1-133	[»]

ProteinModelPortal

P18670.

SMR

P18670. Positions 1-133.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.100.10.30. 1 hit.

InterPro

IPR001229. Mannose-bd_lectin.
[Graphical view]

Pfam

PF01419. Jacalin. 1 hit.
[Graphical view]

SMART

SM00915. Jacalin. 1 hit.
[Graphical view]

SUPFAM

SSF51101. Mannose-bd_lectin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P18670.

Entry information

Entry name

LECA_ARTIN

Accession

Primary (citable) accession number: P18670
Secondary accession number(s): P80023, Q9S788

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	August 1, 1991
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program