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P18670 (LECA_ARTIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Agglutinin alpha chain
Alternative name(s):
Jacalin alpha chain
OrganismArtocarpus integer (Jack fruit) (Artocarpus integrifolia)
Taxonomic identifier3490 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesMoraceaeArtocarpus

Protein attributes

Sequence length133 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.

Subunit structure

Tetramer of four alpha chains associated with two or four beta chains. Ref.3

Sequence similarities

Belongs to the jacalin lectin family.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   DomainRepeat
   LigandIgA-binding protein
Lectin
   PTMGlycoprotein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Molecular_functionIgA binding

Inferred from direct assay Ref.5. Source: UniProtKB

carbohydrate binding

Inferred from direct assay Ref.7. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 133133Agglutinin alpha chain
PRO_0000072798

Regions

Repeat7 – 6458
Repeat76 – 13055
Region68 – 8922IgA-binding

Amino acid modifications

Glycosylation431N-linked (GlcNAc...); when associated with variant T-45; partial Ref.3
Glycosylation741N-linked (GlcNAc...); partial Ref.3

Natural variations

Natural variant311V → I. Ref.3
Natural variant341L → G.
Natural variant451K → L.
Natural variant451K → T. Ref.3
Natural variant661M → D.
Natural variant661M → V. Ref.3
Natural variant671E → M.
Natural variant721T → I.
Natural variant741N → K.
Natural variant1021T → D.
Natural variant1131I → V.
Natural variant1311L → N.

Experimental info

Sequence conflict191Y → I AA sequence Ref.6
Sequence conflict751V → Y AA sequence Ref.2

Secondary structure

......................... 133
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18670 [UniParc].

Last modified August 1, 1991. Version 2.
Checksum: FF10513379CB2E10

FASTA13314,663
        10         20         30         40         50         60 
GKAFDDGAFT GIREINLSYN KETAIGDFQV VYDLNGSPYV GQNHKSFITG FTPVKISLDF 

        70         80         90        100        110        120 
PSEYIMEVSG YTGNVSGYVV VRSLTFKTNK KTYGPYGVTS GTPFNLPIEN GLIVGFKGSI 

       130 
GYWLDYFSMY LSL 

« Hide

References

[1]"The amino acid sequences of jacalin and the Maclura pomifera agglutinin."
Young N.M., Johnston R.A.Z., Watson D.C.
FEBS Lett. 282:382-384(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Seed.
[2]"Primary structure of a Thomsen-Friedenreich-antigen-specific lectin, jacalin [Artocarpus integrifolia (jack fruit) agglutinin]. Evidence for the presence of an internal repeat."
Mahanta S.K., Sanker S., Prasad Rao N.V.S.A.V., Swamy M.J., Surolia A.
Biochem. J. 284:95-101(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Fruit.
[3]"Structural and electron-microscopic studies of jacalin from jackfruit (Artocarpus integrifolia) show that this lectin is a 65 kDa tetramer."
Ruffet E., Paquet N., Frutiger S., Hughes G.J., Jaton J.-C.
Biochem. J. 286:131-134(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, VARIANTS ILE-31; THR-45 AND VAL-66, GLYCOSYLATION AT ASN-43 AND ASN-74, SUBUNIT.
Tissue: Seed.
[4]"Homology of the D-galactose-specific lectins from Artocarpus integrifolia and Maclura pomifera and the role of an unusual small polypeptide subunit."
Young N.M., Johnston R.A.Z., Szabo A.G., Watson D.C.
Arch. Biochem. Biophys. 270:596-603(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-33.
Tissue: Seed.
[5]"Identification of a novel 4 kDa immunoglobulin-A-binding peptide obtained by the limited proteolysis of jacalin."
Kabir S., Aebersold R., Daar A.S.
Biochim. Biophys. Acta 1161:194-200(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-29 AND 68-89.
Tissue: Seed.
[6]"The alpha- and beta-subunits of the jacalins are cleavage products from a 17-kDa precursor."
Ngoc L.D., Brillard M., Hoebeke J.
Biochim. Biophys. Acta 1156:219-222(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-27.
[7]"A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold."
Sakaranarayanan R., Sekar S., Banerjee R., Sharma V., Surolia A., Vijayan M.
Nat. Struct. Biol. 3:596-603(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.43 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRS29641.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JACX-ray2.43A/C/E/G1-133[»]
1KU8X-ray1.75A/C/E/G1-133[»]
1KUJX-ray2.00A/C/E/G1-133[»]
1M26X-ray1.62A/C/E/G1-133[»]
1PXDX-ray1.80A1-133[»]
1TOQX-ray2.50A/C/E/G1-133[»]
1TP8X-ray3.00A/C/E/G1-133[»]
1UGWX-ray1.70A/C/E/G1-133[»]
1UGXX-ray1.60A1-133[»]
1UGYX-ray2.40A/C/E/G1-133[»]
1UH0X-ray2.80A/C/E/G1-133[»]
1UH1X-ray2.80A/C/E/G1-133[»]
1WS4X-ray1.90A/C/E/G1-133[»]
1WS5X-ray1.90A/C/E/G1-133[»]
4AK4X-ray1.65A/C/E/G/I/K/M/O1-133[»]
4AKBX-ray1.95A/C/E/G1-133[»]
4AKCX-ray2.30A/C/E/G1-133[»]
ProteinModelPortalP18670.
SMRP18670. Positions 1-133.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.100.10.30. 1 hit.
InterProIPR001229. Mannose-bd_lectin.
[Graphical view]
PfamPF01419. Jacalin. 1 hit.
[Graphical view]
SMARTSM00915. Jacalin. 1 hit.
[Graphical view]
SUPFAMSSF51101. SSF51101. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP18670.

Entry information

Entry nameLECA_ARTIN
AccessionPrimary (citable) accession number: P18670
Secondary accession number(s): P80023, Q9S788
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: August 1, 1991
Last modified: February 19, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references