P18649 (APOE_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apolipoprotein E
Short name=Apo-E

Gene names

Name:

APOE

Organism

Canis familiaris (Dog) (Canis lupus familiaris)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Subcellular location	Secreted.
Tissue specificity	Present in multiple tissues including liver, jejunum, urinary bladder, ileum, colon, brain, kidney, spleen, pancreas and testis. Secreted in plasma.
Post-translational modification	Phosphorylation sites are present in the extracelllular medium By similarity.
Sequence similarities	Belongs to the apolipoprotein A1/A4/E family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Chylomicron HDL Secreted VLDL
Domain	Repeat
Ligand	Heparin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	induction of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	chylomicron Inferred from electronic annotation. Source: UniProtKB-KW high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW very-low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-amyloid binding Inferred from sequence or structural similarity. Source: UniProtKB heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Apolipoprotein E		PRO_0000191636
Regions
	Repeat	68 – 89	22	1
	Repeat	90 – 111	22	2
	Repeat	112 – 133	22	3
	Repeat	134 – 155	22	4
	Repeat	156 – 177	22	5
	Repeat	178 – 199	22	6
	Repeat	200 – 221	22	7
	Repeat	222 – 243	22	8
	Region	68 – 243	176	8 X 22 AA approximate tandem repeats
	Region	146 – 156	11	LDL receptor binding By similarity
	Region	150 – 153	4	Heparin-binding By similarity
	Region	217 – 224	8	Heparin-binding By similarity
Amino acid modifications
	Modified residue	135	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18649 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 8524E4D3A162F9B1	FASTA	305	35,354
	10 20 30 40 50 60 DVQPEPELER ELEPKVQQEL EPEAGWQTGQ PWEAALARFW DYLRWVQTLS DQVQEGVLNT 70 80 90 100 110 120 QVTQELTALM DETMKEVKAY KAELDEQLGP MTSETQARVA KELQAAQARL RADMEDVRNR 130 140 150 160 170 180 LTQYRGELQA MLGQSSEELR ARFASHMRKL RKRVLRDAED LQRRLAVYKA GVREGAERSV 190 200 210 220 230 240 SSIRERLWPL LEQARERNAK VGALATQPLL ERADAWGQQL RGQLEEMSSR ARGHLEEMRE 250 260 270 280 290 300 QIQEVRVKME EQADQIRQKA EAFQARLKSW FEPLLEDMQR QWDGLVEKVQ AAVATIPTSK PVEEP

References

[1]	"Comparison of the human, canine and swine E apoproteins." Weisgraber K.H., Troxler R.F., Rall S.C., Mahley R.W. Biochem. Biophys. Res. Commun. 95:374-380(1980) [PubMed: 7417263] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20.
[2]	"Structure and expression of dog apolipoprotein A-I, E, and C-I mRNAs: implications for the evolution and functional constraints of apolipoprotein structure." Luo C.-C., Li W.-H., Chan L. J. Lipid Res. 30:1735-1746(1989) [PubMed: 2515239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-305. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	A05310. C60940.
3D structure databases
ProteinModelPortal	P18649.
ModBase	Search...
Protein-protein interaction databases
STRING	P18649.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	maNOG14435.
HOVERGEN	HBG010582.
InParanoid	P18649.
OrthoDB	EOG4GB76S.
Family and domain databases
InterPro	IPR013326. ApoA/E_ApoLp. IPR000074. ApoA1_A4_E. [Graphical view]
Gene3D	G3DSA:1.20.120.20. ApoA/E_ApoLp. 2 hits.
Pfam	PF01442. Apolipoprotein. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APOE_CANFA

Accession

Primary (citable) accession number: P18649

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 15, 1998
Last modified:	September 21, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program