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Protein

Apolipoprotein A-I

Gene

APOA1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-I
Short name:
Apo-AI
Short name:
ApoA-I
Alternative name(s):
Apolipoprotein A1
Cleaved into the following 2 chains:
Gene namesi
Name:APOA1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 265247Proapolipoprotein A-IPRO_0000425336Add
BLAST
Chaini25 – 265241Apolipoprotein A-IPRO_0000001950Add
BLAST
Chaini25 – 264240Truncated apolipoprotein A-IBy similarityPRO_0000416578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091Methionine sulfoxideBy similarity
Modified residuei135 – 1351Methionine sulfoxideBy similarity

Post-translational modificationi

Glycosylated.By similarity
Palmitoylated.By similarity
Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP18648.
PRIDEiP18648.

Expressioni

Tissue specificityi

Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.1 Publication

Developmental stagei

Liver apoa-I expressed in fetal, newborn and suckling animals. Intestinal apoA-I only expressed in postpartum animals.

Gene expression databases

GenevisibleiP18648. SS.

Interactioni

Subunit structurei

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015994.

Structurei

3D structure databases

ProteinModelPortaliP18648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati67 – 88221Add
BLAST
Repeati89 – 110222Add
BLAST
Repeati111 – 121113; half-lengthAdd
BLAST
Repeati122 – 142214Add
BLAST
Repeati144 – 165225Add
BLAST
Repeati166 – 187226Add
BLAST
Repeati188 – 209227Add
BLAST
Repeati210 – 230218Add
BLAST
Repeati231 – 241119; half-lengthAdd
BLAST
Repeati242 – 2652410Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 26519910 X approximate tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IWKR. Eukaryota.
ENOG410YGQ6. LUCA.
HOGENOMiHOG000033998.
HOVERGENiHBG105708.
InParanoidiP18648.
KOiK08757.
OrthoDBiEOG7TBC3N.
TreeFamiTF334458.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVVLTLAV LFLTGSQARH FWQQDDPQSP WDRVKDFATV YVDAIKDSGR
60 70 80 90 100
DYVAQFEASA LGKHLNLKLL DNWDSLGSTF TKVREQLGPV TQEFWDNLEK
110 120 130 140 150
ETEALRQEMS KDLEEVKKKV QPYLDDFQNK WQEEMETYRQ KMAPLGAEFR
160 170 180 190 200
EGARQKVQEL QEKLSPLAEE LRDRLRAHVE ALRQHVAPYS DDLRQRMAAR
210 220 230 240 250
FEALKEGGGS LAEYQAKAQE QLKALGEKAK PALEDLRQGL LPVLENLKVS
260
ILAAIDEASK KLNAQ
Length:265
Mass (Da):30,325
Last modified:February 1, 1994 - v4
Checksum:i2C6E578318ECF69C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081E → K in X17057 (PubMed:2105375).Curated
Sequence conflicti143 – 1431Missing in CAA49234 (PubMed:8294940).Curated
Sequence conflicti143 – 1431Missing in X17057 (PubMed:2105375).Curated
Sequence conflicti173 – 1731D → S in CAA42050 (PubMed:8428656).Curated
Sequence conflicti180 – 1801E → A in CAA49234 (PubMed:8294940).Curated
Sequence conflicti180 – 1801E → A in X17057 (PubMed:2105375).Curated
Sequence conflicti185 – 1862HV → QL in AAA30992 (PubMed:8468059).Curated
Sequence conflicti185 – 1862HV → QL AA sequence (Ref. 3) Curated
Sequence conflicti209 – 2091G → D in CAA49234 (PubMed:8294940).Curated
Sequence conflicti209 – 2091G → D in X17057 (PubMed:2105375).Curated
Sequence conflicti224 – 2241A → G in CAA42050 (PubMed:8428656).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00626 Genomic DNA. Translation: AAA30992.1.
X69477 mRNA. Translation: CAA49234.1.
X17057 mRNA. No translation available.
X59414 mRNA. Translation: CAA42050.1.
PIRiA46018.
JT0672.
S21830.
S31394.
RefSeqiNP_999563.1. NM_214398.1.
UniGeneiSsc.807.

Genome annotation databases

GeneIDi397691.
KEGGissc:397691.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00626 Genomic DNA. Translation: AAA30992.1.
X69477 mRNA. Translation: CAA49234.1.
X17057 mRNA. No translation available.
X59414 mRNA. Translation: CAA42050.1.
PIRiA46018.
JT0672.
S21830.
S31394.
RefSeqiNP_999563.1. NM_214398.1.
UniGeneiSsc.807.

3D structure databases

ProteinModelPortaliP18648.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015994.

Proteomic databases

PaxDbiP18648.
PRIDEiP18648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397691.
KEGGissc:397691.

Organism-specific databases

CTDi335.

Phylogenomic databases

eggNOGiENOG410IWKR. Eukaryota.
ENOG410YGQ6. LUCA.
HOGENOMiHOG000033998.
HOVERGENiHBG105708.
InParanoidiP18648.
KOiK08757.
OrthoDBiEOG7TBC3N.
TreeFamiTF334458.

Gene expression databases

GenevisibleiP18648. SS.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the apolipoprotein AI and CIII genes in the domestic pig."
    Birchbauer A., Knipping G., Juritsch B., Aschauer H., Zechner R.
    Genomics 15:643-652(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Expression of apolipoprotein A-I in porcine brain endothelium in vitro."
    Moeckel B., Zinke H., Flach R., Weiss B., Weiler-Guettler H., Gassen H.
    J. Neurochem. 62:788-798(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain and Liver.
  3. Cited for: PROTEIN SEQUENCE OF 25-265.
  4. "Characterization of the plasma lipoproteins and apoproteins of the Erythrocebus patas monkey."
    Mahley R.W., Weisgraber K.H., Innerarity T., Brewer H.B. Jr.
    Biochemistry 15:1928-1933(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-34.
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 34-265.
    Tissue: Brain.
  6. "Sequences and expression of the porcine apolipoprotein A-I and C-III mRNAs."
    Trieu V.N., Hasler-Rapacz J., Rapacz J., Black D.D.
    Gene 123:173-179(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-265, TISSUE SPECIFICITY.
    Tissue: Liver.
  7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 105-265.

Entry informationi

Entry nameiAPOA1_PIG
AccessioniPrimary (citable) accession number: P18648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1994
Last modified: June 8, 2016
This is version 121 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.