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Protein

Apolipoprotein A-I

Gene

APOA1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-I
Short name:
Apo-AI
Short name:
ApoA-I
Alternative name(s):
Apolipoprotein A1
Cleaved into the following 2 chains:
Gene namesi
Name:APOA1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000042533619 – 265Proapolipoprotein A-IAdd BLAST247
ChainiPRO_000000195025 – 265Apolipoprotein A-IAdd BLAST241
ChainiPRO_000041657825 – 264Truncated apolipoprotein A-IBy similarityAdd BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109Methionine sulfoxideBy similarity1
Modified residuei135Methionine sulfoxideBy similarity1

Post-translational modificationi

Glycosylated.By similarity
Palmitoylated.By similarity
Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP18648.
PeptideAtlasiP18648.
PRIDEiP18648.

Expressioni

Tissue specificityi

Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.1 Publication

Developmental stagei

Liver apoa-I expressed in fetal, newborn and suckling animals. Intestinal apoA-I only expressed in postpartum animals.

Gene expression databases

GenevisibleiP18648. SS.

Interactioni

Subunit structurei

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015994.

Structurei

3D structure databases

ProteinModelPortaliP18648.
SMRiP18648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati67 – 881Add BLAST22
Repeati89 – 1102Add BLAST22
Repeati111 – 1213; half-lengthAdd BLAST11
Repeati122 – 1424Add BLAST21
Repeati144 – 1655Add BLAST22
Repeati166 – 1876Add BLAST22
Repeati188 – 2097Add BLAST22
Repeati210 – 2308Add BLAST21
Repeati231 – 2419; half-lengthAdd BLAST11
Repeati242 – 26510Add BLAST24

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 26510 X approximate tandem repeatsAdd BLAST199

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IWKR. Eukaryota.
ENOG410YGQ6. LUCA.
HOGENOMiHOG000033998.
HOVERGENiHBG105708.
InParanoidiP18648.
KOiK08757.
OrthoDBiEOG091G0IA5.
TreeFamiTF334458.

Family and domain databases

InterProiView protein in InterPro
IPR000074. ApoA_E.
PfamiView protein in Pfam
PF01442. Apolipoprotein. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVVLTLAV LFLTGSQARH FWQQDDPQSP WDRVKDFATV YVDAIKDSGR
60 70 80 90 100
DYVAQFEASA LGKHLNLKLL DNWDSLGSTF TKVREQLGPV TQEFWDNLEK
110 120 130 140 150
ETEALRQEMS KDLEEVKKKV QPYLDDFQNK WQEEMETYRQ KMAPLGAEFR
160 170 180 190 200
EGARQKVQEL QEKLSPLAEE LRDRLRAHVE ALRQHVAPYS DDLRQRMAAR
210 220 230 240 250
FEALKEGGGS LAEYQAKAQE QLKALGEKAK PALEDLRQGL LPVLENLKVS
260
ILAAIDEASK KLNAQ
Length:265
Mass (Da):30,325
Last modified:February 1, 1994 - v4
Checksum:i2C6E578318ECF69C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108E → K in X17057 (PubMed:2105375).Curated1
Sequence conflicti143Missing in CAA49234 (PubMed:8294940).Curated1
Sequence conflicti143Missing in X17057 (PubMed:2105375).Curated1
Sequence conflicti173D → S in CAA42050 (PubMed:8428656).Curated1
Sequence conflicti180E → A in CAA49234 (PubMed:8294940).Curated1
Sequence conflicti180E → A in X17057 (PubMed:2105375).Curated1
Sequence conflicti185 – 186HV → QL in AAA30992 (PubMed:8468059).Curated2
Sequence conflicti185 – 186HV → QL AA sequence (Ref. 3) Curated2
Sequence conflicti209G → D in CAA49234 (PubMed:8294940).Curated1
Sequence conflicti209G → D in X17057 (PubMed:2105375).Curated1
Sequence conflicti224A → G in CAA42050 (PubMed:8428656).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00626 Genomic DNA. Translation: AAA30992.1.
X69477 mRNA. Translation: CAA49234.1.
X17057 mRNA. No translation available.
X59414 mRNA. Translation: CAA42050.1.
PIRiA46018.
JT0672.
S21830.
S31394.
RefSeqiNP_999563.1. NM_214398.1.
UniGeneiSsc.807.

Genome annotation databases

GeneIDi397691.
KEGGissc:397691.

Similar proteinsi

Entry informationi

Entry nameiAPOA1_PIG
AccessioniPrimary (citable) accession number: P18648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1994
Last modified: August 30, 2017
This is version 126 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families