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Protein

UDP-glucose 4-epimerase

Gene

Gale

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-glucose = UDP-alpha-D-galactose.By similarity
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-galactosamine.By similarity

Cofactori

NAD+By similarity

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881NAD; via carbonyl oxygenBy similarity
Binding sitei92 – 921NADBy similarity
Active sitei156 – 1561Proton acceptorBy similarity
Binding sitei160 – 1601NADBy similarity
Binding sitei184 – 1841NADBy similarity
Binding sitei238 – 2381SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 143NADBy similarity
Nucleotide bindingi33 – 375NADBy similarity
Nucleotide bindingi66 – 672NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 4-epimeraseBy similarity (EC:5.1.3.2By similarity)
Alternative name(s):
GalactowaldenaseBy similarity
UDP-N-acetylglucosamine 4-epimeraseBy similarity (EC:5.1.3.7By similarity)
Short name:
UDP-GlcNAc 4-epimeraseBy similarity
UDP-galactosamine 4-epimeraseBy similarity
Short name:
UDP-GalNAc 4-epimeraseBy similarity
UDP-galactose 4-epimeraseBy similarity
Gene namesi
Name:Gale
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621493. Gale.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347UDP-glucose 4-epimerasePRO_0000183191Add
BLAST

Proteomic databases

PaxDbiP18645.
PRIDEiP18645.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013322.

Structurei

3D structure databases

ProteinModelPortaliP18645.
SMRiP18645. Positions 1-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1343Substrate bindingBy similarity
Regioni184 – 1863Substrate bindingBy similarity
Regioni205 – 2073Substrate bindingBy similarity
Regioni223 – 2253Substrate bindingBy similarity
Regioni299 – 3024Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1371. Eukaryota.
COG1087. LUCA.
HOVERGENiHBG001396.
InParanoidiP18645.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
IPR008089. Nuc_sugar_epim.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
PRINTSiPR01713. NUCEPIMERASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01179. galE. 1 hit.

Sequencei

Sequence statusi: Complete.

P18645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKVLVTGG AGYIGSHTVL ELLEAGYSPV VIDNFHNSIR GEDSMPESLR
60 70 80 90 100
RVQELTGRSV EFEEMDILDQ AALQHLFKKH NFKAVIHFAG LKAVGESVQK
110 120 130 140 150
PLDYYRVNLT GTIQLLEIMR AMGVKSLVFS SSATVYGKPV PASGRGPPHR
160 170 180 190 200
GCTKPYGKSK FFIEEMIQDL CRADTAWNAV LLRYFIPIGA HRSARIGEDP
210 220 230 240 250
QGIPNNLMPY VSQVAIGRRE ALNVFGDDYA TEDGTGVRDY IHVVDLAKGH
260 270 280 290 300
IAALKKLKEQ CGCRIYNLGT GTGYSVLQMV QAMEKASGKK IPYKVVARRE
310 320 330 340
GDVAACYANP SLAHEELGWT AALGLDRMCE DLWRWQKQNP SGLGAHG
Length:347
Mass (Da):38,225
Last modified:November 1, 1990 - v1
Checksum:i625B81546E699143
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53949 mRNA. Translation: CAA37897.1.
PIRiS11223.
UniGeneiRn.29908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53949 mRNA. Translation: CAA37897.1.
PIRiS11223.
UniGeneiRn.29908.

3D structure databases

ProteinModelPortaliP18645.
SMRiP18645. Positions 1-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013322.

Proteomic databases

PaxDbiP18645.
PRIDEiP18645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi621493. Gale.

Phylogenomic databases

eggNOGiKOG1371. Eukaryota.
COG1087. LUCA.
HOVERGENiHBG001396.
InParanoidiP18645.

Enzyme and pathway databases

UniPathwayiUPA00214.

Miscellaneous databases

PROiP18645.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
IPR008089. Nuc_sugar_epim.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
PRINTSiPR01713. NUCEPIMERASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01179. galE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGALE_RAT
AccessioniPrimary (citable) accession number: P18645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: December 9, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.