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Protein

23S rRNA (adenosine(1067)-2'-O)-methyltransferase

Gene

tsnR

Organism
Streptomyces azureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the adenosine-1067 in 23S ribosomal RNA. Confers resistance to antibiotic thiostrepton.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + adenosine(1067) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyladenosine(1067) in 23S rRNA.1 Publication

Kineticsi

  1. KM=0.15 mM for S-adenosyl-L-methionine1 Publication

    pH dependencei

    Optimum pH is 7.5-7.6.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei135 – 1351S-adenosyl-L-methionineCombined sources
    Binding sitei165 – 1651S-adenosyl-L-methionineCombined sources
    Binding sitei195 – 1951S-adenosyl-L-methionine; via carbonyl oxygenCombined sources
    Binding sitei238 – 2381S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenCombined sources
    Binding sitei252 – 2521S-adenosyl-L-methionineCombined sources

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16697.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    23S rRNA (adenosine(1067)-2'-O)-methyltransferaseCurated (EC:2.1.1.2301 Publication)
    Alternative name(s):
    23S rRNA [AM1067] 2'-O-methyltransferase
    Short name:
    23S rRNA methylase
    Thiostrepton-resistance methylase1 Publication
    rRNA (adenosine-2'-O)-methyltransferase
    Gene namesi
    Name:tsnR
    Synonyms:tsr1 Publication
    OrganismiStreptomyces azureus
    Taxonomic identifieri146537 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi88 – 881F → A: Defective in RNA binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 26926923S rRNA (adenosine(1067)-2'-O)-methyltransferasePRO_0000065669Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1
    269
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi16 – 183Combined sources
    Helixi19 – 224Combined sources
    Beta strandi23 – 253Combined sources
    Beta strandi30 – 345Combined sources
    Helixi37 – 448Combined sources
    Turni45 – 473Combined sources
    Beta strandi49 – 5911Combined sources
    Turni63 – 664Combined sources
    Helixi67 – 704Combined sources
    Turni71 – 733Combined sources
    Beta strandi76 – 794Combined sources
    Helixi81 – 844Combined sources
    Turni85 – 873Combined sources
    Beta strandi96 – 1016Combined sources
    Helixi108 – 1136Combined sources
    Beta strandi118 – 1236Combined sources
    Helixi127 – 13913Combined sources
    Beta strandi143 – 1497Combined sources
    Helixi158 – 1636Combined sources
    Turni164 – 1663Combined sources
    Helixi167 – 1693Combined sources
    Beta strandi173 – 1764Combined sources
    Helixi178 – 1869Combined sources
    Turni187 – 1893Combined sources
    Beta strandi191 – 1955Combined sources
    Beta strandi200 – 2023Combined sources
    Helixi203 – 2086Combined sources
    Beta strandi213 – 2208Combined sources
    Helixi226 – 2283Combined sources
    Turni229 – 2313Combined sources
    Beta strandi234 – 2374Combined sources
    Helixi249 – 25810Combined sources
    Helixi261 – 2644Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3GYQX-ray2.45A/B1-269[»]
    ProteinModelPortaliP18644.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18644.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2203S-adenosyl-L-methionine bindingCombined sources
    Regioni246 – 2472S-adenosyl-L-methionine bindingCombined sources

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.1280.10. 1 hit.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR006795. Thiostrepton-R_Mease_TSNR_N.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view]
    PfamiPF00588. SpoU_methylase. 1 hit.
    PF04705. TSNR_N. 1 hit.
    [Graphical view]
    ProDomiPD407686. Thiostrepton-R_Mease_TSNR_N. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF75217. SSF75217. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18644-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTELDTIANP SDPAVQRIID VTKPSRSNIK TTLIEDVEPL MHSIAAGVEF
    60 70 80 90 100
    IEVYGSDSSP FPSELLDLCG RQNIPVRLID SSIVNQLFKG ERKAKTFGIA
    110 120 130 140 150
    RVPRPARFGD IASRRGDVVV LDGVKIVGNI GAIVRTSLAL GASGIILVDS
    160 170 180 190 200
    DITSIADRRL QRASRGYVFS LPVVLSGREE AIAFIRDSGM QLMTLKADGD
    210 220 230 240 250
    ISVKELGDNP DRLALLFGSE KGGPSDLFEE ASSASVSIPM MSQTESLNVS
    260
    VSLGIALHER IDRNLAANR
    Length:269
    Mass (Da):28,901
    Last modified:November 1, 1990 - v1
    Checksum:i66B1976014A05636
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X02392 Genomic DNA. Translation: CAA26234.1.
    X54219 Genomic DNA. Translation: CAA38132.1.
    PIRiS28369.
    RefSeqiWP_059415847.1. NZ_DF968219.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X02392 Genomic DNA. Translation: CAA26234.1.
    X54219 Genomic DNA. Translation: CAA38132.1.
    PIRiS28369.
    RefSeqiWP_059415847.1. NZ_DF968219.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3GYQX-ray2.45A/B1-269[»]
    ProteinModelPortaliP18644.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16697.

    Miscellaneous databases

    EvolutionaryTraceiP18644.

    Family and domain databases

    Gene3Di3.40.1280.10. 1 hit.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR006795. Thiostrepton-R_Mease_TSNR_N.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view]
    PfamiPF00588. SpoU_methylase. 1 hit.
    PF04705. TSNR_N. 1 hit.
    [Graphical view]
    ProDomiPD407686. Thiostrepton-R_Mease_TSNR_N. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF75217. SSF75217. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces."
      Bibb M.J., Bibb M.J., Ward J.M., Cohen S.N.
      Mol. Gen. Genet. 199:26-36(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 14921 / DSM 40106 / CBS 467.68 / ETH 28555 / JCM 4564 / NBRC 12744 / NRRL B-2655 / VKM Ac-719.
    2. "Tandem promoters, tsrp1 and tsrp2, direct transcription of the thiostrepton resistance gene (tsr) of Streptomyces azureus: transcriptional initiation from tsrp2 occurs after deletion of the -35 region."
      Janssen G.R., Bibb M.J.
      Mol. Gen. Genet. 221:339-346(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
      Strain: ATCC 14921 / DSM 40106 / CBS 467.68 / ETH 28555 / JCM 4564 / NBRC 12744 / NRRL B-2655 / VKM Ac-719.
    3. "Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton."
      Thompson J., Cundliffe E.
      J. Gen. Microbiol. 124:291-297(1981)
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    4. "Site of action of a ribosomal RNA methylase conferring resistance to thiostrepton."
      Thompson J., Schmidt F., Cundliffe E.
      J. Biol. Chem. 257:7915-7917(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    5. "Structure of the thiostrepton resistance methyltransferase.S-adenosyl-L-methionine complex and its interaction with ribosomal RNA."
      Dunstan M.S., Hang P.C., Zelinskaya N.V., Honek J.F., Conn G.L.
      J. Biol. Chem. 284:17013-17020(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE, SUBUNIT, MUTAGENESIS OF PHE-88.

    Entry informationi

    Entry nameiTSNR_STRAJ
    AccessioniPrimary (citable) accession number: P18644
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: May 11, 2016
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.