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Protein

23S rRNA (adenosine(1067)-2'-O)-methyltransferase

Gene

tsnR

Organism
Streptomyces azureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the adenosine-1067 in 23S ribosomal RNA. Confers resistance to antibiotic thiostrepton.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + adenosine(1067) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyladenosine(1067) in 23S rRNA.1 Publication

Kineticsi

  1. KM=0.15 mM for S-adenosyl-L-methionine1 Publication

    pH dependencei

    Optimum pH is 7.5-7.6.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei135S-adenosyl-L-methionineCombined sources1
    Binding sitei165S-adenosyl-L-methionineCombined sources1
    Binding sitei195S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1
    Binding sitei238S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenCombined sources1
    Binding sitei252S-adenosyl-L-methionineCombined sources1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16697.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    23S rRNA (adenosine(1067)-2'-O)-methyltransferaseCurated (EC:2.1.1.2301 Publication)
    Alternative name(s):
    23S rRNA [AM1067] 2'-O-methyltransferase
    Short name:
    23S rRNA methylase
    Thiostrepton-resistance methylase1 Publication
    rRNA (adenosine-2'-O)-methyltransferase
    Gene namesi
    Name:tsnR
    Synonyms:tsr1 Publication
    OrganismiStreptomyces azureus
    Taxonomic identifieri146537 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi88F → A: Defective in RNA binding. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000656691 – 26923S rRNA (adenosine(1067)-2'-O)-methyltransferaseAdd BLAST269

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1269
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi16 – 18Combined sources3
    Helixi19 – 22Combined sources4
    Beta strandi23 – 25Combined sources3
    Beta strandi30 – 34Combined sources5
    Helixi37 – 44Combined sources8
    Turni45 – 47Combined sources3
    Beta strandi49 – 59Combined sources11
    Turni63 – 66Combined sources4
    Helixi67 – 70Combined sources4
    Turni71 – 73Combined sources3
    Beta strandi76 – 79Combined sources4
    Helixi81 – 84Combined sources4
    Turni85 – 87Combined sources3
    Beta strandi96 – 101Combined sources6
    Helixi108 – 113Combined sources6
    Beta strandi118 – 123Combined sources6
    Helixi127 – 139Combined sources13
    Beta strandi143 – 149Combined sources7
    Helixi158 – 163Combined sources6
    Turni164 – 166Combined sources3
    Helixi167 – 169Combined sources3
    Beta strandi173 – 176Combined sources4
    Helixi178 – 186Combined sources9
    Turni187 – 189Combined sources3
    Beta strandi191 – 195Combined sources5
    Beta strandi200 – 202Combined sources3
    Helixi203 – 208Combined sources6
    Beta strandi213 – 220Combined sources8
    Helixi226 – 228Combined sources3
    Turni229 – 231Combined sources3
    Beta strandi234 – 237Combined sources4
    Helixi249 – 258Combined sources10
    Helixi261 – 264Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3GYQX-ray2.45A/B1-269[»]
    ProteinModelPortaliP18644.
    SMRiP18644.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18644.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni218 – 220S-adenosyl-L-methionine bindingCombined sources3
    Regioni246 – 247S-adenosyl-L-methionine bindingCombined sources2

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.1280.10. 1 hit.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR006795. Thiostrepton-R_Mease_TSNR_N.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view]
    PfamiPF00588. SpoU_methylase. 1 hit.
    PF04705. TSNR_N. 1 hit.
    [Graphical view]
    ProDomiPD407686. Thiostrepton-R_Mease_TSNR_N. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF75217. SSF75217. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18644-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTELDTIANP SDPAVQRIID VTKPSRSNIK TTLIEDVEPL MHSIAAGVEF
    60 70 80 90 100
    IEVYGSDSSP FPSELLDLCG RQNIPVRLID SSIVNQLFKG ERKAKTFGIA
    110 120 130 140 150
    RVPRPARFGD IASRRGDVVV LDGVKIVGNI GAIVRTSLAL GASGIILVDS
    160 170 180 190 200
    DITSIADRRL QRASRGYVFS LPVVLSGREE AIAFIRDSGM QLMTLKADGD
    210 220 230 240 250
    ISVKELGDNP DRLALLFGSE KGGPSDLFEE ASSASVSIPM MSQTESLNVS
    260
    VSLGIALHER IDRNLAANR
    Length:269
    Mass (Da):28,901
    Last modified:November 1, 1990 - v1
    Checksum:i66B1976014A05636
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X02392 Genomic DNA. Translation: CAA26234.1.
    X54219 Genomic DNA. Translation: CAA38132.1.
    PIRiS28369.
    RefSeqiWP_059415847.1. NZ_DF968219.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X02392 Genomic DNA. Translation: CAA26234.1.
    X54219 Genomic DNA. Translation: CAA38132.1.
    PIRiS28369.
    RefSeqiWP_059415847.1. NZ_DF968219.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3GYQX-ray2.45A/B1-269[»]
    ProteinModelPortaliP18644.
    SMRiP18644.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16697.

    Miscellaneous databases

    EvolutionaryTraceiP18644.

    Family and domain databases

    Gene3Di3.40.1280.10. 1 hit.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR006795. Thiostrepton-R_Mease_TSNR_N.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view]
    PfamiPF00588. SpoU_methylase. 1 hit.
    PF04705. TSNR_N. 1 hit.
    [Graphical view]
    ProDomiPD407686. Thiostrepton-R_Mease_TSNR_N. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF75217. SSF75217. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTSNR_STRAJ
    AccessioniPrimary (citable) accession number: P18644
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: November 2, 2016
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.