P18637 (MBHS_RHILV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uptake hydrogenase small subunit
EC=1.12.99.6

Alternative name(s):
Hydrogenlyase
Membrane-bound hydrogenase small subunit

Gene names

Name:	hupA
Synonyms:	hupS

Organism

Rhizobium leguminosarum bv. viciae

Taxonomic identifier

387 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 46	46	Tat-type signal Potential
	Chain	47 – 360	314	Uptake hydrogenase small subunit		PRO_0000013434
Sites
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	65	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	160	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	194	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	232	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	235	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	260	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	266	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	275	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	294	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	297	1	Iron-sulfur 3 (3Fe-4S) By similarity
Experimental info
	Sequence conflict	160	1	C → S in CAA85430. Ref.1
	Sequence conflict	222	1	R → P in CAA85430. Ref.1
	Sequence conflict	228 – 230	3	SQR → AQP in CAA85430. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P18637 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: 85304E046F3DAAAF	FASTA	360	39,149
	10 20 30 40 50 60 MATAETFYDV IRRQGITRRS FTKFCSLTAA SLGFGPGAAT AMAEALETKE RVPVIWMHGL 70 80 90 100 110 120 ECTCCSESFI RSAHPLVKDV VLSMISLDYD DTIMAAAGHQ AESILAETKE KYKGKYILAV 130 140 150 160 170 180 EGNPPLNEGG MFCIDGGKPF VEKLKWMAED AMAIIAWGAC ASWGCVQAAK PNPTQATPID 190 200 210 220 230 240 KVILDKPIIK VPGCPPIAEV MTGVVTFITT FGKLPELDRQ GRPKMFYSQR IHDKCYRRPH 250 260 270 280 290 300 FDAGQFVEEW DDEGARKGYC LYKMGCKGPT TYNACSTVRW NGGVSFPIQS GHGCIGCSED 310 320 330 340 350 360 GFWDNGSFYD RLTNIHQFGI EANADKVGMT AAGVVGGAIA AHAAVTAVKR LTTKREKADA

References

[1]	"DNA sequence encoding the two structural genes for the uptake hydrogenase of Rhizobium leguminosarum bv. viciae B10." Schneider C.G., Schmitt H.J., Schild C., Tichy H.V., Lotz W. Nucleic Acids Res. 18:5285-5285(1990) [PubMed: 2402452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B10.
[2]	"Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum." Hidalgo E., Leyva A., Ruiz-Argueso T. Plant Mol. Biol. 15:367-370(1990) [PubMed: 2103457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 128c53.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36981 Genomic DNA. Translation: CAA85430.1. X52974 Genomic DNA. Translation: CAA37148.1.
PIR	S11968.
3D structure databases
ProteinModelPortal	P18637.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. HydA. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHS_RHILV

Accession

Primary (citable) accession number: P18637

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 28, 2003
Last modified:	September 21, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program