Uptake hydrogenase small subunit precursor - Rhizobium leguminosarum bv. viciae

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Reviewed, UniProtKB/Swiss-Prot P18637 (MBHS_RHILV)

Last modified October 13, 2009. Version 77. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit

Gene names

Name:	hupA
Synonyms:	hupS

Organism

Rhizobium leguminosarum bv. viciae

Taxonomic identifier

387 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

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Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	This enzyme recycles the H₂ produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O₂ under carbon- or phosphate-limited conditions.
Catalytic activity	H₂ + A = AH₂.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 46

Tat-type signal Potential

Chain

47 – 360

314

Uptake hydrogenase small subunit

PRO_0000013434

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

160

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

194

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

232

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

235

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

260

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

266

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

275

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

294

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

297

Iron-sulfur 3 (3Fe-4S) By similarity

Experimental info

Sequence conflict

160

C → S in CAA85430. Ref.1

Sequence conflict

222

R → P in CAA85430. Ref.1

Sequence conflict

228 – 230

SQR → AQP in CAA85430. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P18637-1 [UniParc].

Last modified February 28, 2003. Version 2.
Checksum: 85304E046F3DAAAF
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FASTA

360

39,149

        10         20         30         40         50         60 
MATAETFYDV IRRQGITRRS FTKFCSLTAA SLGFGPGAAT AMAEALETKE RVPVIWMHGL 

        70         80         90        100        110        120 
ECTCCSESFI RSAHPLVKDV VLSMISLDYD DTIMAAAGHQ AESILAETKE KYKGKYILAV 

       130        140        150        160        170        180 
EGNPPLNEGG MFCIDGGKPF VEKLKWMAED AMAIIAWGAC ASWGCVQAAK PNPTQATPID 

       190        200        210        220        230        240 
KVILDKPIIK VPGCPPIAEV MTGVVTFITT FGKLPELDRQ GRPKMFYSQR IHDKCYRRPH 

       250        260        270        280        290        300 
FDAGQFVEEW DDEGARKGYC LYKMGCKGPT TYNACSTVRW NGGVSFPIQS GHGCIGCSED 

       310        320        330        340        350        360 
GFWDNGSFYD RLTNIHQFGI EANADKVGMT AAGVVGGAIA AHAAVTAVKR LTTKREKADA

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References

[1]	"DNA sequence encoding the two structural genes for the uptake hydrogenase of Rhizobium leguminosarum bv. viciae B10." Schneider C.G., Schmitt H.J., Schild C., Tichy H.V., Lotz W. Nucleic Acids Res. 18:5285-5285(1990) [PubMed: 2402452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B10.
[2]	"Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum." Hidalgo E., Leyva A., Ruiz-Argueso T. Plant Mol. Biol. 15:367-370(1990) [PubMed: 2103457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 128c53.

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Cross-references

Sequence databases
	Z36981 Genomic DNA. Translation: CAA85430.1. X52974 Genomic DNA. Translation: CAA37148.1.
PIR	S11968.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 267396.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MBHS_RHILV

Accession

Primary (citable) accession number: P18637

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 28, 2003
Last modified:	October 13, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents