P18637 (MBHS_RHILV) Reviewed, UniProtKB/Swiss-Prot
Last modified
September 21, 2011.
Version 90.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Uptake hydrogenase small subunit EC=1.12.99.6 Alternative name(s): Hydrogenlyase Membrane-bound hydrogenase small subunit | ||||
| Gene names |
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| Organism | Rhizobium leguminosarum bv. viciae | ||||
| Taxonomic identifier | 387 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium |
Protein attributes
| Sequence length | 360 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions. |
| Catalytic activity | H2 + A = AH2. |
| Cofactor | Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | |
| Post-translational modification | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | Signal |
| Ligand | 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Cellular component | ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster bindingInferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activityInferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activityInferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW quinone bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 46 | 46 | Tat-type signal Potential | ||||||
| Chain | 47 – 360 | 314 | Uptake hydrogenase small subunit | PRO_0000013434 | |||||
Sites | |||||||||
| Metal binding | 62 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 65 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 160 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 194 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 232 | 1 | Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity | ||||||
| Metal binding | 235 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 260 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 266 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 275 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 294 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
| Metal binding | 297 | 1 | Iron-sulfur 3 (3Fe-4S) By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 160 | 1 | C → S in CAA85430. Ref.1 | ||||||
| Sequence conflict | 222 | 1 | R → P in CAA85430. Ref.1 | ||||||
| Sequence conflict | 228 – 230 | 3 | SQR → AQP in CAA85430. Ref.1 | ||||||
Sequences
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References
| [1] | "DNA sequence encoding the two structural genes for the uptake hydrogenase of Rhizobium leguminosarum bv. viciae B10." Schneider C.G., Schmitt H.J., Schild C., Tichy H.V., Lotz W. Nucleic Acids Res. 18:5285-5285(1990) [PubMed: 2402452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B10. |
| [2] | "Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum." Hidalgo E., Leyva A., Ruiz-Argueso T. Plant Mol. Biol. 15:367-370(1990) [PubMed: 2103457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 128c53. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z36981 Genomic DNA. Translation: CAA85430.1. X52974 Genomic DNA. Translation: CAA37148.1. |
| PIR | S11968. |
3D structure databases | |
| ProteinModelPortal | P18637. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. [Graphical view] |
| Gene3D | G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit. |
| Pfam | PF01058. Oxidored_q6. 1 hit. [Graphical view] |
| PIRSF | PIRSF000310. NiFe_hyd_ssu. 1 hit. |
| PRINTS | PR00614. NIHGNASESMLL. |
| TIGRFAMs | TIGR00391. HydA. 1 hit. |
| PROSITE | PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHS_RHILV | ||||||||
| Accession | Primary (citable) accession number: P18637 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with