P18636 (MBHL_RHILV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uptake hydrogenase large subunit
EC=1.12.99.6

Alternative name(s):
Hydrogenlyase
Membrane-bound hydrogenase large subunit

Gene names

Name:	hupB
Synonyms:	hupL

Organism

Rhizobium leguminosarum bv. viciae

Taxonomic identifier

387 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Uptake hydrogenase large subunit		PRO_0000199715
Sites
	Metal binding	75	1	Nickel Potential
	Metal binding	78	1	Nickel Potential
	Metal binding	575	1	Nickel Potential
	Metal binding	578	1	Nickel Potential
Experimental info
	Sequence conflict	510	1	R → A in CAA37149. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18636 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 474EA9B9FD2CBF68	FASTA	596	66,193
	10 20 30 40 50 60 MTIQTPNGFT LDNSGKRIVV DPVTRIEGHM RVEVNVDENN IIRNAVSTGT MWRGIEVILK 70 80 90 100 110 120 NRDPRDAWAF TERICGVCTG THALTSVRAV ENALGITIPD NANSIRNLMQ LALQVHDHVV 130 140 150 160 170 180 HFYHLHALDW VDVVSALSAD PKATSALAQS ISDWPLSSPG YFKDIQTRLK KFVESGQLGP 190 200 210 220 230 240 FKNGYWGNAS YKLPPEANLM AVAHYLEALD FQKEIVKIHT IFGGKNPHPN WLVGGVPCPI 250 260 270 280 290 300 NVDGTGAVGA INMERLNMVT SIIDQLIEFN DKVYVPDIMA IGSFYKDWLY GGGLSGKNVL 310 320 330 340 350 360 AYGDVPEHAN DYSEASLKLP RGAIINGNLA EVFPVDHADP EQIQEFVTHS WYKYPDESKG 370 380 390 400 410 420 LHPWDGITEP HYELGPNAKG TKTNIEQLDE GAKYSWIKAP RWRGNAMEVG PLARWVIGYA 430 440 450 460 470 480 QNKAEFKDPV DKVLKDLGLP VTALFSTLGR TAARALESQW AGYQMRYFQN KLIANIKAGD 490 500 510 520 530 540 SNTAFVDKWK PETWPKEVKG VGFTEAPRGR LAHWIRIKDG KIDNYQCVVP TTWNGSPRDP 550 560 570 580 590 TGNIGAFEAS LMDTPMSNPT QPLEILRTIH SFDPCLACST HVMSPDGQEM ARVQVR

References

[1]	"DNA sequence encoding the two structural genes for the uptake hydrogenase of Rhizobium leguminosarum bv. viciae B10." Schneider C.G., Schmitt H.J., Schild C., Tichy H.V., Lotz W. Nucleic Acids Res. 18:5285-5285(1990) [PubMed: 2402452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B10.
[2]	"Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum." Hidalgo E., Leyva A., Ruiz-Argueso T. Plant Mol. Biol. 15:367-370(1990) [PubMed: 2103457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 128c53.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36981 Genomic DNA. Translation: CAA85431.1. X52974 Genomic DNA. Translation: CAA37149.1.
PIR	S11969.
3D structure databases
ProteinModelPortal	P18636.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_RHILV

Accession

Primary (citable) accession number: P18636

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program