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Reviewed, UniProtKB/Swiss-Prot P18636 (MBHL_RHILV)

Last modified November 4, 2008. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Uptake hydrogenase large subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase large subunit
Gene names
Name: hupB
Synonyms: hupL
OrganismRhizobium leguminosarum bv. viciae
Taxonomic identifier387 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

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Protein attributes

Sequence length596 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.

Catalytic activity

H(2) + A = AH(2).

Cofactor

Binds 1 nickel ion per subunit By similarity.

Subunit structure

Heterodimer of a large and a small subunit.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

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Ontologies

Keywords

   Cellular componentCell membrane
Membrane
   LigandMetal-binding
Nickel
   Molecular functionOxidoreductase

Gene Ontology (GO)

   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionhydrogenase (acceptor) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 596596Uptake hydrogenase large subunit
PRO_0000199715

Sites

Metal binding751Nickel Potential
Metal binding781Nickel Potential
Metal binding5751Nickel Potential
Metal binding5781Nickel Potential

Experimental info

Sequence conflict5101R → A in CAA37149. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P18636-1 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 474EA9B9FD2CBF68

FASTA59666,193
        10         20         30         40         50         60 
MTIQTPNGFT LDNSGKRIVV DPVTRIEGHM RVEVNVDENN IIRNAVSTGT MWRGIEVILK 

        70         80         90        100        110        120 
NRDPRDAWAF TERICGVCTG THALTSVRAV ENALGITIPD NANSIRNLMQ LALQVHDHVV 

       130        140        150        160        170        180 
HFYHLHALDW VDVVSALSAD PKATSALAQS ISDWPLSSPG YFKDIQTRLK KFVESGQLGP 

       190        200        210        220        230        240 
FKNGYWGNAS YKLPPEANLM AVAHYLEALD FQKEIVKIHT IFGGKNPHPN WLVGGVPCPI 

       250        260        270        280        290        300 
NVDGTGAVGA INMERLNMVT SIIDQLIEFN DKVYVPDIMA IGSFYKDWLY GGGLSGKNVL 

       310        320        330        340        350        360 
AYGDVPEHAN DYSEASLKLP RGAIINGNLA EVFPVDHADP EQIQEFVTHS WYKYPDESKG 

       370        380        390        400        410        420 
LHPWDGITEP HYELGPNAKG TKTNIEQLDE GAKYSWIKAP RWRGNAMEVG PLARWVIGYA 

       430        440        450        460        470        480 
QNKAEFKDPV DKVLKDLGLP VTALFSTLGR TAARALESQW AGYQMRYFQN KLIANIKAGD 

       490        500        510        520        530        540 
SNTAFVDKWK PETWPKEVKG VGFTEAPRGR LAHWIRIKDG KIDNYQCVVP TTWNGSPRDP 

       550        560        570        580        590 
TGNIGAFEAS LMDTPMSNPT QPLEILRTIH SFDPCLACST HVMSPDGQEM ARVQVR

« Hide

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References

[1]"DNA sequence encoding the two structural genes for the uptake hydrogenase of Rhizobium leguminosarum bv. viciae B10."
Schneider C.G., Schmitt H.J., Schild C., Tichy H.V., Lotz W.
Nucleic Acids Res. 18:5285-5285(1990) [PubMed: 2402452] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: B10.
[2]"Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum."
Hidalgo E., Leyva A., Ruiz-Argueso T.
Plant Mol. Biol. 15:367-370(1990) [PubMed: 2103457] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 128c53.

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Cross-references

Sequence databases

Z36981 Genomic DNA. Translation: CAA85431.1.
X52974 Genomic DNA. Translation: CAA37149.1.
PIRS11969.

3D structure databases

HSSPHSSP built from PDB template 1H2R based on UniProtKB P21852.
ModBaseSearch...

Family and domain databases

InterProIPR001501. Ni-dep_hyd_lsu.
[Graphical view]
PfamPF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
PROSITEPS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

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Entry information

Entry nameMBHL_RHILV
AccessionPrimary (citable) accession number: P18636
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 4, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents