Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P18632

- PLY1_CRYJA

UniProt

P18632 - PLY1_CRYJA

Protein

Pectate lyase 1

Gene
N/A
Organism
Cryptomeria japonica (Japanese cedar) (Cupressus japonica)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 3 (16 Apr 2014)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Has pectate lyase activity.1 Publication

    Catalytic activityi

    Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.1 Publication

    Cofactori

    Binds 1 calcium ion.1 Publication

    pH dependencei

    Optimum pH is 10.1 Publication

    Temperature dependencei

    Optimum temperature is 60-70 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi170 – 1701CalciumBy similarity
    Metal bindingi194 – 1941CalciumBy similarity
    Metal bindingi198 – 1981CalciumBy similarity
    Active sitei250 – 2501Sequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pectate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pectin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00545; UER00824.

    Protein family/group databases

    CAZyiPL1. Polysaccharide Lyase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectate lyase 1 (EC:4.2.2.2)
    Alternative name(s):
    Allergen Cry j I
    Major allergen Cry j 1
    Sugi basic protein
    Short name:
    SBP
    Allergen: Cry j 1
    OrganismiCryptomeria japonica (Japanese cedar) (Cupressus japonica)
    Taxonomic identifieri3369 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeCryptomeria

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. This is one of the major allergens of Japanese cedar pollen, the most common pollen allergen in Japan.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei248. Cry j 1.
    3223. Cry j 1.0101.
    3224. Cry j 1.0102.
    3225. Cry j 1.0103.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21211 PublicationAdd
    BLAST
    Chaini22 – 374353Pectate lyase 1PRO_0000024906Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 45By similarity
    Disulfide bondi128 ↔ 147By similarity
    Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi191 – 1911N-linked (GlcNAc...) (complex)1 PublicationCAR_000135
    Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi306 ↔ 312By similarity
    Glycosylationi354 – 3541N-linked (GlcNAc...) (complex)1 PublicationCAR_000136

    Post-translational modificationi

    N-glycosylated; contains fucose and xylose.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    PTM databases

    UniCarbKBiP18632.

    Structurei

    3D structure databases

    ProteinModelPortaliP18632.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    PfamiPF00544. Pec_lyase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00807. AMBALLERGEN.
    SMARTiSM00656. Amb_all. 1 hit.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18632-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSPCLVALL VFSFVIGSCF SDNPIDSCWR GDSNWAQNRM KLADCAVGFG    50
    SSTMGGKGGD LYTVTNSDDD PVNPAPGTLR YGATRDRPLW IIFSGNMNIK 100
    LKMPMYIAGY KTFDGRGAQV YIGNGGPCVF IKRVSNVIIH GLYLYGCSTS 150
    VLGNVLINES FGVEPVHPQD GDALTLRTAT NIWIDHNSFS NSSDGLVDVT 200
    LTSTGVTISN NLFFNHHKVM LLGHDDAYSD DKSMKVTVAF NQFGPNCGQR 250
    MPRARYGLVH VANNNYDPWT IYAIGGSSNP TILSEGNSFT APNESYKKQV 300
    TIRIGCKTSS SCSNWVWQST QDVFYNGAYF VSSGKYEGGN IYTKKEAFNV 350
    ENGNATPQLT KNAGVLTCSL SKRC 374
    Length:374
    Mass (Da):40,720
    Last modified:April 16, 2014 - v3
    Checksum:i90D0085D24BF2BD4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121F → L in BAA05542. (PubMed:8135802)Curated
    Sequence conflicti143 – 1431Y → H in BAA05542. (PubMed:8135802)Curated
    Sequence conflicti202 – 2021T → S in BAA05542. (PubMed:8135802)Curated
    Sequence conflicti221 – 2211L → S in BAA05543. (PubMed:8135802)Curated
    Sequence conflicti358 – 3581Q → H in BAA05543. (PubMed:8135802)Curated
    Sequence conflicti361 – 3611K → Q in BAA05543. (PubMed:8135802)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26544 mRNA. Translation: BAA05542.1.
    D26545 mRNA. Translation: BAA05543.1.
    D34639 mRNA. Translation: BAA07020.1.
    AB081309 mRNA. Translation: BAB86286.1.
    AB081310 mRNA. Translation: BAB86287.1.
    PIRiA44773.
    JC2123.
    JC2124.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26544 mRNA. Translation: BAA05542.1 .
    D26545 mRNA. Translation: BAA05543.1 .
    D34639 mRNA. Translation: BAA07020.1 .
    AB081309 mRNA. Translation: BAB86286.1 .
    AB081310 mRNA. Translation: BAB86287.1 .
    PIRi A44773.
    JC2123.
    JC2124.

    3D structure databases

    ProteinModelPortali P18632.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 248. Cry j 1.
    3223. Cry j 1.0101.
    3224. Cry j 1.0102.
    3225. Cry j 1.0103.
    CAZyi PL1. Polysaccharide Lyase Family 1.

    PTM databases

    UniCarbKBi P18632.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00545 ; UER00824 .

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    Pfami PF00544. Pec_lyase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00807. AMBALLERGEN.
    SMARTi SM00656. Amb_all. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of cDNA coding for Cry j I, a major allergen of Japanese cedar pollen."
      Sone T., Komiyama N., Shimizu K., Kusakabe T., Morikubo K., Kino K.
      Biochem. Biophys. Res. Commun. 199:619-625(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Pollen.
    2. Namba M., Kurose M., Torigoe K., Fukuda S., Kurimoto M.
      Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pollen.
    3. "Isolation and characterization of cDNAs encoding major allergen Cry j 1 from Cryptomeria japonica pollen."
      Futamura N., Shinohara K.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pollen.
    4. "N-terminal amino acid sequence of a major allergen of Japanese cedar pollen (Cry j I)."
      Taniai M., Ando S., Usui M., Kurimoto M., Sakaguchi M., Inouye S., Matuhasi T.
      FEBS Lett. 239:329-332(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-41.
      Tissue: Pollen.
    5. "Cry j I, a major allergen of Japanese cedar pollen, has pectate lyase enzyme activity."
      Taniguchi Y., Ono A., Sawatani M., Nanba M., Kohno K., Usui M., Kurimoto M., Matuhasi T.
      Allergy 50:90-93(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 250-266, FUNCTION AS A PECTATE LYASE, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
      Tissue: Pollen.
    6. "Antigenicity of the oligosaccharide moiety of the Japanese cedar (Cryptomeria japonica) pollen allergen, Cry jI."
      Hijikata A., Matsumoto I., Kojima K., Ogawa H.
      Int. Arch. Allergy Immunol. 105:198-202(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-191 AND ASN-354.
      Tissue: Pollen.
    7. "Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen."
      Hino K., Yamamoto S., Sano O., Taniguchi Y., Kohno K., Usui M., Fukuda S., Hanzawa H., Haruyama H., Kurimoto M.
      J. Biochem. 117:289-295(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE OF CARBOHYDRATES.
      Tissue: Pollen.

    Entry informationi

    Entry nameiPLY1_CRYJA
    AccessioniPrimary (citable) accession number: P18632
    Secondary accession number(s): Q8RUR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 79 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3