P18632 (SBP_CRYJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sugi basic protein Short name=SBP Alternative name(s): Allergen Cry j I Major allergen Cry j 1 Allergen=Cry j 1
Organism	Cryptomeria japonica (Japanese cedar) (Cupressus japonica)
Taxonomic identifier	3369 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Coniferophyta › Coniferopsida › Coniferales › Cupressaceae › Cryptomeria

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Post-translational modification	N-glycosylated; contains fucose and xylose. Ref.4 Ref.5
Allergenic properties	Causes an allergic reaction in human. This is one of the major allergens of Japanese cedar pollen, the most common pollen allergen in Japan.
Miscellaneous	The sequence of Cry j 1 form A is shown here. Form B differs in six positions.
Sequence similarities	Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
Disease	Allergen
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.3
	Chain	22 – 374	353	Sugi basic protein		PRO_0000024906
Amino acid modifications
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) (complex) Ref.4		CAR_000135
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	354	1	N-linked (GlcNAc...) (complex) Ref.4		CAR_000136
	Disulfide bond	28 ↔ 45		By similarity
	Disulfide bond	128 ↔ 147		By similarity
	Disulfide bond	306 ↔ 312		By similarity
Natural variations
	Natural variant	12	1	L → F in Cry j 1-B.
	Natural variant	143	1	H → Y in Cry j 1-B.
	Natural variant	202	1	S → T in Cry j 1-B.
	Natural variant	221	1	L → S in Cry j 1-B.
	Natural variant	358	1	Q → H in Cry j 1-B.
	Natural variant	361	1	K → Q in Cry j 1-B.

Sequences

	Sequence		Length	Mass (Da)
	P18632 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 74AB25950248F56F	FASTA	374	40,646
	10 20 30 40 50 60 MDSPCLVALL VLSFVIGSCF SDNPIDSCWR GDSNWAQNRM KLADCAVGFG SSTMGGKGGD 70 80 90 100 110 120 LYTVTNSDDD PVNPAPGTLR YGATRDRPLW IIFSGNMNIK LKMPMYIAGY KTFDGRGAQV 130 140 150 160 170 180 YIGNGGPCVF IKRVSNVIIH GLHLYGCSTS VLGNVLINES FGVEPVHPQD GDALTLRTAT 190 200 210 220 230 240 NIWIDHNSFS NSSDGLVDVT LSSTGVTISN NLFFNHHKVM LLGHDDAYSD DKSMKVTVAF 250 260 270 280 290 300 NQFGPNCGQR MPRARYGLVH VANNNYDPWT IYAIGGSSNP TILSEGNSFT APNESYKKQV 310 320 330 340 350 360 TIRIGCKTSS SCSNWVWQST QDVFYNGAYF VSSGKYEGGN IYTKKEAFNV ENGNATPQLT 370 KNAGVLTCSL SKRC

References

[1]	"Cloning and sequencing of cDNA coding for Cry j I, a major allergen of Japanese cedar pollen." Sone T., Komiyama N., Shimizu K., Kusakabe T., Morikubo K., Kino K. Biochem. Biophys. Res. Commun. 199:619-625(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Pollen.
[2]	Namba M., Kurose M., Torigoe K., Fukuda S., Kurimoto M. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.
[3]	"N-terminal amino acid sequence of a major allergen of Japanese cedar pollen (Cry j I)." Taniai M., Ando S., Usui M., Kurimoto M., Sakaguchi M., Inouye S., Matuhasi T. FEBS Lett. 239:329-332(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-41. Tissue: Pollen.
[4]	"Antigenicity of the oligosaccharide moiety of the Japanese cedar (Cryptomeria japonica) pollen allergen, Cry jI." Hijikata A., Matsumoto I., Kojima K., Ogawa H. Int. Arch. Allergy Immunol. 105:198-202(1994) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-191 AND ASN-354. Tissue: Pollen.
[5]	"Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen." Hino K., Yamamoto S., Sano O., Taniguchi Y., Kohno K., Usui M., Fukuda S., Hanzawa H., Haruyama H., Kurimoto M. J. Biochem. 117:289-295(1995) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATES. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26544 mRNA. Translation: BAA05542.1. D26545 mRNA. Translation: BAA05543.1. D34639 mRNA. Translation: BAA07020.1.
PIR	A44773. JC2123. JC2124.
3D structure databases
ProteinModelPortal	P18632.
SMR	P18632. Positions 22-367.
ModBase	Search...
Protein family/group databases
Allergome	248. Cry j 1. 3223. Cry j 1.0101. 3224. Cry j 1.0102.
CAZy	PL1. Polysaccharide Lyase Family 1.
PTM databases
GlycoSuiteDB	P18632.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.160.20.10. 1 hit.
InterPro	IPR002022. Amb_allergen_dom. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SBP_CRYJA

Accession

Primary (citable) accession number: P18632

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program