ID   LAG3_HUMAN              Reviewed;         525 AA.
AC   P18627; A8K7T9;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 5.
DT   25-JAN-2012, entry version 109.
DE   RecName: Full=Lymphocyte activation gene 3 protein;
DE            Short=LAG-3;
DE   AltName: Full=Protein FDC;
DE   AltName: CD_antigen=CD223;
DE   Flags: Precursor;
GN   Name=LAG3; Synonyms=FDC;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT THR-455.
RX   MEDLINE=90237736; PubMed=1692078; DOI=10.1084/jem.171.5.1393;
RA   Triebel F., Jitsukawa S., Baixeras E., Roman-Roman S., Genevee C.,
RA   Viegas-Pequignot E., Hercend T.;
RT   "LAG-3, a novel lymphocyte activation gene closely related to CD4.";
RL   J. Exp. Med. 171:1393-1405(1990).
RN   [2]
RP   SEQUENCE REVISION TO C-TERMINUS.
RA   Triebel F.;
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-455.
RC   TISSUE=Synovium;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16541075; DOI=10.1038/nature04569;
RA   Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA   Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA   Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M.,
RA   Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA   Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B.,
RA   Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D.,
RA   Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z.,
RA   Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z.,
RA   Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H.,
RA   Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H.,
RA   Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V.,
RA   Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J.,
RA   Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A.,
RA   Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M.,
RA   Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E.,
RA   Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA   Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA   Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M.,
RA   Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R.,
RA   Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J.,
RA   Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C.,
RA   Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M.,
RA   Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M.,
RA   Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P.,
RA   Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L.,
RA   Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E.,
RA   Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C.,
RA   Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F.,
RA   Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M.,
RA   Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S.,
RA   Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA   Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA   Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA   Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA   Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA   Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D.,
RA   Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I.,
RA   Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T.,
RA   Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S.,
RA   Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D.,
RA   Kucherlapati R., Weinstock G., Gibbs R.A.;
RT   "The finished DNA sequence of human chromosome 12.";
RL   Nature 440:346-351(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT THR-455.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   CHARACTERIZATION.
RX   MEDLINE=92364535; PubMed=1380059; DOI=10.1084/jem.176.2.327;
RA   Baixeras E., Huard B., Miossec C., Jitsukawa S., Martin M.,
RA   Hercend T., Auffray C., Triebel F., Piatier-Tonneau D.;
RT   "Characterization of the lymphocyte activation gene 3-encoded protein.
RT   A new ligand for human leukocyte antigen class II antigens.";
RL   J. Exp. Med. 176:327-337(1992).
CC   -!- FUNCTION: Involved in lymphocyte activation. Binds to HLA class-II
CC       antigens.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane
CC       protein.
CC   -!- TISSUE SPECIFICITY: On cell surface of activated NK and T-
CC       lymphocytes.
CC   -!- SIMILARITY: Contains 3 Ig-like C2-type (immunoglobulin-like)
CC       domains.
CC   -!- SIMILARITY: Contains 1 Ig-like V-type (immunoglobulin-like)
CC       domain.
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DR   EMBL; X51985; CAA36243.3; -; mRNA.
DR   EMBL; AK292104; BAF84793.1; -; mRNA.
DR   EMBL; AC125494; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471116; EAW88741.1; -; Genomic_DNA.
DR   IPI; IPI00000881; -.
DR   PIR; S11246; S11246.
DR   RefSeq; NP_002277.4; NM_002286.5.
DR   UniGene; Hs.409523; -.
DR   ProteinModelPortal; P18627; -.
DR   SMR; P18627; 33-66, 128-261, 266-384.
DR   IntAct; P18627; 3.
DR   STRING; P18627; -.
DR   PhosphoSite; P18627; -.
DR   DMDM; 251757512; -.
DR   PRIDE; P18627; -.
DR   Ensembl; ENST00000203629; ENSP00000203629; ENSG00000089692.
DR   GeneID; 3902; -.
DR   KEGG; hsa:3902; -.
DR   CTD; 3902; -.
DR   GeneCards; GC12P006881; -.
DR   H-InvDB; HIX0201912; -.
DR   HGNC; HGNC:6476; LAG3.
DR   HPA; CAB002301; -.
DR   HPA; HPA013967; -.
DR   MIM; 153337; gene.
DR   neXtProt; NX_P18627; -.
DR   PharmGKB; PA30265; -.
DR   eggNOG; prNOG09709; -.
DR   GeneTree; ENSGT00390000017331; -.
DR   HOGENOM; HBG126621; -.
DR   HOVERGEN; HBG004952; -.
DR   InParanoid; P18627; -.
DR   OMA; ESPHHHL; -.
DR   OrthoDB; EOG480HWM; -.
DR   PhylomeDB; P18627; -.
DR   NextBio; 15317; -.
DR   ArrayExpress; P18627; -.
DR   Bgee; P18627; -.
DR   CleanEx; HS_LAG3; -.
DR   Genevestigator; P18627; -.
DR   GermOnline; ENSG00000089692; Homo sapiens.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003823; F:antigen binding; TAS:ProtInc.
DR   GO; GO:0042289; F:MHC class II protein binding; IDA:MGI.
DR   InterPro; IPR007110; Ig-like.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR015621; IL1-receptor.
DR   Gene3D; G3DSA:2.60.40.10; Ig-like_fold; 2.
DR   KO; K06565; -.
DR   PANTHER; PTHR11890; IL1-receptor; 1.
DR   SMART; SM00409; IG; 3.
DR   PROSITE; PS50835; IG_LIKE; 2.
PE   1: Evidence at protein level;
KW   Complete proteome; Disulfide bond; Glycoprotein;
KW   Immunoglobulin domain; Membrane; Polymorphism; Reference proteome;
KW   Repeat; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL        1     28
FT   CHAIN        29    525       Lymphocyte activation gene 3 protein.
FT                                /FTId=PRO_0000014631.
FT   TOPO_DOM     29    450       Extracellular (Potential).
FT   TRANSMEM    451    471       Helical; (Potential).
FT   TOPO_DOM    472    525       Cytoplasmic (Potential).
FT   DOMAIN       37    167       Ig-like V-type.
FT   DOMAIN      168    252       Ig-like C2-type 1.
FT   DOMAIN      265    343       Ig-like C2-type 2.
FT   DOMAIN      348    419       Ig-like C2-type 3.
FT   REGION      501    524       12 X 2 AA tandem repeats of E-X.
FT   CARBOHYD    188    188       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    250    250       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    256    256       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    343    343       N-linked (GlcNAc...) (Potential).
FT   DISULFID     44    160       Potential.
FT   DISULFID    189    241       Potential.
FT   DISULFID    282    333       Potential.
FT   DISULFID    369    412       Potential.
FT   VARIANT     455    455       I -> T (in dbSNP:rs870849).
FT                                /FTId=VAR_058295.
SQ   SEQUENCE   525 AA;  57449 MW;  5E0BDEF20E9E36D2 CRC64;
     MWEAQFLGLL FLQPLWVAPV KPLQPGAEVP VVWAQEGAPA QLPCSPTIPL QDLSLLRRAG
     VTWQHQPDSG PPAAAPGHPL APGPHPAAPS SWGPRPRRYT VLSVGPGGLR SGRLPLQPRV
     QLDERGRQRG DFSLWLRPAR RADAGEYRAA VHLRDRALSC RLRLRLGQAS MTASPPGSLR
     ASDWVILNCS FSRPDRPASV HWFRNRGQGR VPVRESPHHH LAESFLFLPQ VSPMDSGPWG
     CILTYRDGFN VSIMYNLTVL GLEPPTPLTV YAGAGSRVGL PCRLPAGVGT RSFLTAKWTP
     PGGGPDLLVT GDNGDFTLRL EDVSQAQAGT YTCHIHLQEQ QLNATVTLAI ITVTPKSFGS
     PGSLGKLLCE VTPVSGQERF VWSSLDTPSQ RSFSGPWLEA QEAQLLSQPW QCQLYQGERL
     LGAAVYFTEL SSPGAQRSGR APGALPAGHL LLFLILGVLS LLLLVTGAFG FHLWRRQWRP
     RRFSALEQGI HPPQAQSKIE ELEQEPEPEP EPEPEPEPEP EPEQL
//