Reviewed, UniProtKB/Swiss-Prot P18627 (LAG3_HUMAN)

Names and origin

Protein names

Recommended name:
    Lymphocyte activation gene 3 protein
      Short name=LAG-3
Alternative name(s):
    FDC protein
    CD_antigen=CD223

Gene names

Name:	LAG3
Synonyms:	FDC

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in lymphocyte activation. Binds to HLA class-II antigens.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	On cell surface of activated NK and T-lymphocytes.
Sequence similarities	Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Repeat Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	MHC class II protein binding Inferred from direct assay. Source: MGI antigen binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28
	Chain	29 – 525	497	Lymphocyte activation gene 3 protein		PRO_0000014631
Regions
	Topological domain	29 – 450	422	Extracellular Potential
	Transmembrane	451 – 471	21	Potential
	Topological domain	472 – 525	54	Cytoplasmic Potential
	Domain	37 – 167	131	Ig-like V-type
	Domain	168 – 252	85	Ig-like C2-type 1
	Domain	265 – 343	79	Ig-like C2-type 2
	Domain	348 – 419	72	Ig-like C2-type 3
	Region	501 – 524	24	12 X 2 AA tandem repeats of E-X
Amino acid modifications
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	343	1	N-linked (GlcNAc...) Potential
	Disulfide bond	44 ↔ 160		Potential
	Disulfide bond	189 ↔ 241		Potential
	Disulfide bond	282 ↔ 333		Potential
	Disulfide bond	369 ↔ 412		Potential

Sequences

	Sequence		Length	Mass (Da)
	P18627-1 [UniParc]. Last modified December 19, 2001. Version 4. Checksum: C711EDDB0E9E2733	FASTA	525	57,437
	10 20 30 40 50 60 MWEAQFLGLL FLQPLWVAPV KPLQPGAEVP VVWAQEGAPA QLPCSPTIPL QDLSLLRRAG 70 80 90 100 110 120 VTWQHQPDSG PPAAAPGHPL APGPHPAAPS SWGPRPRRYT VLSVGPGGLR SGRLPLQPRV 130 140 150 160 170 180 QLDERGRQRG DFSLWLRPAR RADAGEYRAA VHLRDRALSC RLRLRLGQAS MTASPPGSLR 190 200 210 220 230 240 ASDWVILNCS FSRPDRPASV HWFRNRGQGR VPVRESPHHH LAESFLFLPQ VSPMDSGPWG 250 260 270 280 290 300 CILTYRDGFN VSIMYNLTVL GLEPPTPLTV YAGAGSRVGL PCRLPAGVGT RSFLTAKWTP 310 320 330 340 350 360 PGGGPDLLVT GDNGDFTLRL EDVSQAQAGT YTCHIHLQEQ QLNATVTLAI ITVTPKSFGS 370 380 390 400 410 420 PGSLGKLLCE VTPVSGQERF VWSSLDTPSQ RSFSGPWLEA QEAQLLSQPW QCQLYQGERL 430 440 450 460 470 480 LGAAVYFTEL SSPGAQRSGR APGALPAGHL LLFLTLGVLS LLLLVTGAFG FHLWRRQWRP 490 500 510 520 RRFSALEQGI HPPQAQSKIE ELEQEPEPEP EPEPEPEPEP EPEQL

References

[1]	"LAG-3, a novel lymphocyte activation gene closely related to CD4." Triebel F., Jitsukawa S., Baixeras E., Roman-Roman S., Genevee C., Viegas-Pequignot E., Hercend T. J. Exp. Med. 171:1393-1405(1990) [PubMed: 1692078] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Triebel F. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens." Baixeras E., Huard B., Miossec C., Jitsukawa S., Martin M., Hercend T., Auffray C., Triebel F., Piatier-Tonneau D. J. Exp. Med. 176:327-337(1992) [PubMed: 1380059] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51985 mRNA. Translation: CAA36243.3.
IPI	IPI00000881.
PIR	S11246.
RefSeq	NP_002277.4.
UniGene	Hs.409523
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P18627.
Genome annotation databases
Ensembl	ENSG00000089692. Homo sapiens. [Contig view]
GeneID	3902.
KEGG	hsa:3902.
Organism-specific databases
GeneCards	GC12P006751.
H-InvDB	HIX0026391.
HGNC	HGNC:6476. LAG3.
HPA	CAB002301. HPA013967.
MIM	153337. gene.
PharmGKB	PA30265.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P18627.
HOVERGEN	P18627.
Gene expression databases
ArrayExpress	P18627.
Bgee	P18627.
CleanEx	HS_LAG3.
GermOnline	ENSG00000089692. Homo sapiens.
Family and domain databases
InterPro	IPR013151. Ig. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. IPR015621. IL1-receptor. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR11890. IL1-receptor. 1 hit.
Pfam	PF00047. ig. 1 hit. PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 3 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	15317.
SOURCE	Search...

Entry information

Entry name

LAG3_HUMAN

Accession

Primary (citable) accession number: P18627

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 19, 2001
Last modified:	March 3, 2009
This is version 85 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)