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Protein

Lymphocyte activation gene 3 protein

Gene

LAG3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in lymphocyte activation. Binds to HLA class-II antigens.

GO - Molecular functioni

  • antigen binding Source: ProtInc
  • MHC class II protein binding Source: MGI
  • transmembrane signaling receptor activity Source: Ensembl

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-2132295. MHC class II antigen presentation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte activation gene 3 protein
Short name:
LAG-3
Alternative name(s):
Protein FDC
CD_antigen: CD223
Gene namesi
Name:LAG3
Synonyms:FDC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000089692.8.
HGNCiHGNC:6476. LAG3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 450ExtracellularSequence analysisAdd BLAST422
Transmembranei451 – 471HelicalSequence analysisAdd BLAST21
Topological domaini472 – 525CytoplasmicSequence analysisAdd BLAST54

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi3902.
OpenTargetsiENSG00000089692.
PharmGKBiPA30265.

Polymorphism and mutation databases

BioMutaiLAG3.
DMDMi251757512.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Add BLAST28
ChainiPRO_000001463129 – 525Lymphocyte activation gene 3 proteinAdd BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44 ↔ 160PROSITE-ProRule annotation
Glycosylationi188N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi189 ↔ 241PROSITE-ProRule annotation
Glycosylationi250N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi282 ↔ 333PROSITE-ProRule annotation
Glycosylationi343N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi369 ↔ 412PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP18627.
PeptideAtlasiP18627.
PRIDEiP18627.

PTM databases

PhosphoSitePlusiP18627.

Expressioni

Tissue specificityi

On cell surface of activated NK and T-lymphocytes.

Gene expression databases

BgeeiENSG00000089692.
CleanExiHS_LAG3.
GenevisibleiP18627. HS.

Organism-specific databases

HPAiCAB002301.
HPA013967.

Interactioni

GO - Molecular functioni

  • MHC class II protein binding Source: MGI

Protein-protein interaction databases

BioGridi110097. 14 interactors.
IntActiP18627. 3 interactors.
STRINGi9606.ENSP00000203629.

Structurei

3D structure databases

ProteinModelPortaliP18627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 167Ig-like V-typeAdd BLAST131
Domaini168 – 252Ig-like C2-type 1Add BLAST85
Domaini265 – 343Ig-like C2-type 2Add BLAST79
Domaini348 – 419Ig-like C2-type 3Add BLAST72

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni501 – 52412 X 2 AA tandem repeats of E-XAdd BLAST24

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFPP. Eukaryota.
ENOG410Z3KP. LUCA.
GeneTreeiENSGT00390000017331.
HOGENOMiHOG000113275.
HOVERGENiHBG004952.
InParanoidiP18627.
KOiK06565.
OMAiERFVWSP.
OrthoDBiEOG091G0PJF.
PhylomeDBiP18627.
TreeFamiTF335942.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiView protein in InterPro
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR015621. IL-1_rcpt_fam.
PANTHERiPTHR11890. PTHR11890. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 3 hits.
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P18627-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWEAQFLGLL FLQPLWVAPV KPLQPGAEVP VVWAQEGAPA QLPCSPTIPL
60 70 80 90 100
QDLSLLRRAG VTWQHQPDSG PPAAAPGHPL APGPHPAAPS SWGPRPRRYT
110 120 130 140 150
VLSVGPGGLR SGRLPLQPRV QLDERGRQRG DFSLWLRPAR RADAGEYRAA
160 170 180 190 200
VHLRDRALSC RLRLRLGQAS MTASPPGSLR ASDWVILNCS FSRPDRPASV
210 220 230 240 250
HWFRNRGQGR VPVRESPHHH LAESFLFLPQ VSPMDSGPWG CILTYRDGFN
260 270 280 290 300
VSIMYNLTVL GLEPPTPLTV YAGAGSRVGL PCRLPAGVGT RSFLTAKWTP
310 320 330 340 350
PGGGPDLLVT GDNGDFTLRL EDVSQAQAGT YTCHIHLQEQ QLNATVTLAI
360 370 380 390 400
ITVTPKSFGS PGSLGKLLCE VTPVSGQERF VWSSLDTPSQ RSFSGPWLEA
410 420 430 440 450
QEAQLLSQPW QCQLYQGERL LGAAVYFTEL SSPGAQRSGR APGALPAGHL
460 470 480 490 500
LLFLILGVLS LLLLVTGAFG FHLWRRQWRP RRFSALEQGI HPPQAQSKIE
510 520
ELEQEPEPEP EPEPEPEPEP EPEQL
Length:525
Mass (Da):57,449
Last modified:July 7, 2009 - v5
Checksum:i5E0BDEF20E9E36D2
GO
Isoform 2 (identifier: P18627-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-360: VTPKSFGS → GQPQVGKE
     361-525: Missing.

Note: No experimental confirmation available.
Show »
Length:360
Mass (Da):39,103
Checksum:iC3FDD5C8A4EB4215
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_058295455I → T3 PublicationsCorresponds to variant dbSNP:rs870849Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056311353 – 360VTPKSFGS → GQPQVGKE in isoform 2. 1 Publication8
Alternative sequenceiVSP_056312361 – 525Missing in isoform 2. 1 PublicationAdd BLAST165

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51985 mRNA. Translation: CAA36243.3.
AK292104 mRNA. Translation: BAF84793.1.
AC125494 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88741.1.
BC052589 mRNA. Translation: AAH52589.1.
CCDSiCCDS8561.1. [P18627-1]
PIRiS11246.
RefSeqiNP_002277.4. NM_002286.5. [P18627-1]
UniGeneiHs.409523.

Genome annotation databases

EnsembliENST00000203629; ENSP00000203629; ENSG00000089692. [P18627-1]
ENST00000441671; ENSP00000413825; ENSG00000089692. [P18627-2]
GeneIDi3902.
KEGGihsa:3902.
UCSCiuc001qqs.4. human. [P18627-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiLAG3_HUMAN
AccessioniPrimary (citable) accession number: P18627
Secondary accession number(s): A8K7T9, Q7Z643
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 7, 2009
Last modified: September 27, 2017
This is version 157 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot