P18608 (HMGN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-histone chromosomal protein HMG-14

Alternative name(s):
High mobility group nucleosome-binding domain-containing protein 1

Gene names

Name:	Hmgn1
Synonyms:	Hmg-14, Hmg14

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2. Ref.5
Subcellular location	Nucleus. Cytoplasm By similarity. Note: Cytoplasmic enrichment upon phosphorylation By similarity.
Post-translational modification	Phosphorylation favors cytoplasmic localization By similarity. Phosphorylation on Ser-20 and Ser-24 weakens binding to nucleosomes and increases the rate of H3 phosphorylation.
Sequence similarities	Belongs to the HMGN family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chromatin organization Inferred from mutant phenotype PubMed 12660172. Source: MGI post-embryonic camera-type eye morphogenesis Inferred from mutant phenotype PubMed 16466397. Source: MGI pyrimidine dimer repair by nucleotide-excision repair Inferred from mutant phenotype PubMed 12660172. Source: MGI regulation of development, heterochronic Inferred from genetic interaction PubMed 11133167. Source: MGI regulation of epithelial cell proliferation Inferred from mutant phenotype PubMed 16466397. Source: MGI regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 21278158. Source: MGI response to UV-B Inferred from mutant phenotype PubMed 12660172. Source: MGI response to UV-C Inferred from direct assay PubMed 12660172. Source: MGI transcription-coupled nucleotide-excision repair Inferred from mutant phenotype PubMed 12660172. Source: MGI
Cellular_component	chromatin Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay PubMed 11133167PubMed 16466397. Source: MGI
Molecular_function	chromatin binding Inferred from direct assay PubMed 12660172. Source: MGI nucleosomal DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 96	95	Non-histone chromosomal protein HMG-14		PRO_0000206692
Amino acid modifications
	Modified residue	7	1	Phosphoserine; by RPS6KA5 Ref.4 Ref.5 Ref.7
	Modified residue	13	1	N6-acetyllysine By similarity
	Modified residue	20	1	Phosphoserine; by RPS6KA5 Ref.5
	Modified residue	24	1	Phosphoserine; by RPS6KA5 Ref.5
	Modified residue	80	1	Phosphothreonine Ref.6
	Modified residue	84	1	Phosphoserine Ref.6
	Modified residue	87	1	Phosphoserine Ref.6 Ref.7
	Modified residue	95	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P18608 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 65C4C09DE0973786	FASTA	96	10,152
	10 20 30 40 50 60 MPKRKVSADG AAKAEPKRRS ARLSAKPAPA KVDAKPKKAA GKDKASDKKV QIKGKRGAKG 70 80 90 KQADVADQQT TELPAENGET ENQSPASEEE KEAKSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse non-histone chromosomal protein HMG-14 cDNA sequence." Landsman D., Bustin M. Nucleic Acids Res. 18:5311-5311(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye and Limb.
[3]	"Selective decrease in low-Mr HMG proteins HMG I and HMG Y during differentiation of mouse teratocarcinoma cells." Vartiainen E., Palvimo J., Mahonen A., Linnala-Kankkunen A., Maeenpaeae P.H. FEBS Lett. 228:45-48(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-19.
[4]	"A mitogen- and anisomycin-stimulated kinase phosphorylates HMG-14 in its basic amino-terminal domain in vivo and on isolated mononucleosomes." Barratt M.J., Hazzalin C.A., Zhelev N., Mahadevan L.C. EMBO J. 13:4524-4535(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-7.
[5]	"Chromosomal protein HMGN1 modulates histone H3 phosphorylation." Lim J.-H., Catez F., Birger Y., West K.L., Prymakowska-Bosak M., Postnikov Y.V., Bustin M. Mol. Cell 15:573-584(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT SER-7; SER-20 AND SER-24.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-80; SER-84; SER-87 AND SER-95, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND SER-87, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53476 mRNA. Translation: CAA37569.1. BC083138 mRNA. Translation: AAH83138.1. BC088834 mRNA. Translation: AAH88834.1.
IPI	IPI00338745.
PIR	S11219.
RefSeq	NP_032277.3. NM_008251.3. XP_001472107.1. XM_001472057.3.
UniGene	Mm.2756.
3D structure databases
ProteinModelPortal	P18608.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000061012.
PTM databases
PhosphoSite	P18608.
Proteomic databases
PaxDb	P18608.
PRIDE	P18608.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050884; ENSMUSP00000061012; ENSMUSG00000040681.
GeneID	100044391. 15312.
KEGG	mmu:100044391. mmu:15312.
Organism-specific databases
CTD	3150.
MGI	MGI:96120. Hmgn1.
Phylogenomic databases
eggNOG	NOG29368.
GeneTree	ENSGT00700000104489.
HOGENOM	HOG000116394.
HOVERGEN	HBG073479.
InParanoid	P18608.
KO	K11299.
OMA	TKTEENP.
Gene expression databases
ArrayExpress	P18608.
Bgee	P18608.
CleanEx	MM_HMGN1.
Genevestigator	P18608.
GermOnline	ENSMUSG00000040681. Mus musculus.
Family and domain databases
InterPro	IPR000079. HMGN_fam. [Graphical view]
PANTHER	PTHR23087. PTHR23087. 1 hit.
Pfam	PF01101. HMG14_17. 1 hit. [Graphical view]
PRINTS	PR00925. NONHISHMG17.
SMART	SM00527. HMG17. 1 hit. [Graphical view]
PROSITE	PS00355. HMG14_17. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HMGN1. mouse.
NextBio	287951.
SOURCE	Search...

Entry information

Entry name

HMGN1_MOUSE

Accession

Primary (citable) accession number: P18608
Secondary accession number(s): Q5HZY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program