P18598 (ATP4B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium-transporting ATPase subunit beta

Alternative name(s):
Gastric H(+)/K(+) ATPase subunit beta
Proton pump beta chain

Gene names

Name:

Atp4b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subunit structure	Composed of two subunits: alpha (catalytic) and beta.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Stomach.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro gastric acid secretion Traceable author statement Ref.1. Source: RGD response to lipopolysaccharide Inferred from expression pattern. Source: RGD response to organic nitrogen Inferred from expression pattern. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrogen:potassium-exchanging ATPase activity Traceable author statement Ref.1. Source: RGD sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Potassium-transporting ATPase subunit beta		PRO_0000219095
Regions
	Topological domain	1 – 36	36	Cytoplasmic Potential
	Transmembrane	37 – 57	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	58 – 294	237	Extracellular Potential
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Disulfide bond	131 ↔ 152		By similarity
	Disulfide bond	162 ↔ 178		By similarity
	Disulfide bond	201 ↔ 266		By similarity
Experimental info
	Sequence conflict	136	1	Y → H in AAA41330. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P18598 [UniParc]. Last modified April 1, 1993. Version 3. Checksum: EE640D88835AD8AF	FASTA	294	33,689
	10 20 30 40 50 60 MAALQEKKSC SQRMAEFRQY CWNPDTGQML GRTPARWVWI SLYYAAFYVV MTGLFALCIY 70 80 90 100 110 120 VLMQTIDPYT PDYQDQLKSP GVTLRPDVYG ERGLQISYNI SENSSWAGLT HTLHSFLAGY 130 140 150 160 170 180 TPASQQDSIN CSSEKYFFQE TFSAPNHTKF SCKFTADMLQ NCSGLVDPSF GFEEGKPCFI 190 200 210 220 230 240 IKMNRIVKFL PSNNTAPRVD CTFQDDPQKP RKDIEPLQVQ YYPPNGTFSL HYFPYYGKKA 250 260 270 280 290 QPHYSNPLVA AKFLNVPKNT QVLIVCKIMA DHVTFDNPHD PYEGKVEFKL TIQK

References

[1]	"cDNA cloning of the beta-subunit of the rat gastric H,K-ATPase." Shull G.E. J. Biol. Chem. 265:12123-12126(1990) [PubMed: 2165052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Stomach.
[2]	"Rat gastric H,K-ATPase beta-subunit gene: intron/exon organization, identification of multiple transcription initiation sites, and analysis of the 5'-flanking region." Newman P.R., Shull G.E. Genomics 11:252-262(1991) [PubMed: 1663070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of the H,K-ATPase beta subunit. Tissue-specific expression, chromosomal assignment, and relationship to Na,K-ATPase beta subunits." Canfield V.A., Okamoto C.T., Chow D., Dorfman J., Gros P., Forte J.G., Levenson R. J. Biol. Chem. 265:19878-19884(1990) [PubMed: 1978834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gastric mucosa.
[4]	"The rat H+/K(+)-ATPase beta subunit gene and recognition of its control region by gastric DNA binding protein." Maeda M., Oshiman K.I., Tamura S., Kaya S., Mahmood S., Reuben M.A., Lasater L.S., Sachs G., Futai M. J. Biol. Chem. 266:21584-21588(1991) [PubMed: 1657972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L34666 , L34661, L34662, L34663, L34664, L34665 Genomic DNA. Translation: AAA41332.1. S76404 Genomic DNA. Translation: AAB21120.1. M55655 mRNA. Translation: AAA41330.1. M35535 mRNA. Translation: AAA63482.1.
IPI	IPI00324511.
PIR	A41199.
RefSeq	NP_036642.2. NM_012510.2.
UniGene	Rn.10033.
3D structure databases
ProteinModelPortal	P18598.
ModBase	Search...
Protein-protein interaction databases
STRING	P18598.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025958; ENSRNOP00000025957; ENSRNOG00000018543.
GeneID	24217.
KEGG	rno:24217.
UCSC	NM_012510. rat.
Organism-specific databases
CTD	496.
RGD	2178. Atp4b.
Phylogenomic databases
eggNOG	roNOG06059.
GeneTree	ENSGT00550000074530.
HOVERGEN	HBG050603.
InParanoid	P18598.
OMA	MNRIVKF.
OrthoDB	EOG4TXBS9.
PhylomeDB	P18598.
Gene expression databases
ArrayExpress	P18598.
Genevestigator	P18598.
GermOnline	ENSRNOG00000018543. Rattus norvegicus.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
KO	K01543.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	602643.

Entry information

Entry name

ATP4B_RAT

Accession

Primary (citable) accession number: P18598

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	April 1, 1993
Last modified:	November 16, 2011
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program