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P18596

- AT2A3_RAT

UniProt

P18596 - AT2A3_RAT

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Protein

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Gene
Atp2a3
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi304 – 3041Calcium 2; via carbonyl oxygen By similarity
Metal bindingi305 – 3051Calcium 2; via carbonyl oxygen By similarity
Metal bindingi307 – 3071Calcium 2; via carbonyl oxygen By similarity
Metal bindingi309 – 3091Calcium 2 By similarity
Active sitei351 – 35114-aspartylphosphate intermediate By similarity
Binding sitei515 – 5151ATP By similarity
Metal bindingi703 – 7031Magnesium By similarity
Metal bindingi707 – 7071Magnesium By similarity
Metal bindingi768 – 7681Calcium 1 By similarity
Metal bindingi771 – 7711Calcium 1 By similarity
Metal bindingi796 – 7961Calcium 2 By similarity
Metal bindingi799 – 7991Calcium 1 By similarity
Metal bindingi800 – 8001Calcium 1 By similarity
Metal bindingi800 – 8001Calcium 2 By similarity
Metal bindingi908 – 9081Calcium 1 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium-transporting ATPase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transmembrane transport Source: GOC
  2. calcium ion transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 (EC:3.6.3.8)
Short name:
SERCA3
Short name:
SR Ca(2+)-ATPase 3
Alternative name(s):
Calcium pump 3
Gene namesi
Name:Atp2a3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2175. Atp2a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848Cytoplasmic By similarityAdd
BLAST
Transmembranei49 – 6921Helical; Name=1; By similarityAdd
BLAST
Topological domaini70 – 8920Lumenal By similarityAdd
BLAST
Transmembranei90 – 11021Helical; Name=2; By similarityAdd
BLAST
Topological domaini111 – 253143Cytoplasmic By similarityAdd
BLAST
Transmembranei254 – 27320Helical; Name=3; By similarityAdd
BLAST
Topological domaini274 – 29522Lumenal By similarityAdd
BLAST
Transmembranei296 – 31318Helical; Name=4; By similarityAdd
BLAST
Topological domaini314 – 757444Cytoplasmic By similarityAdd
BLAST
Transmembranei758 – 77720Helical; Name=5; By similarityAdd
BLAST
Topological domaini778 – 78710Lumenal By similarity
Transmembranei788 – 80821Helical; Name=6; By similarityAdd
BLAST
Topological domaini809 – 82820Cytoplasmic By similarityAdd
BLAST
Transmembranei829 – 85123Helical; Name=7; By similarityAdd
BLAST
Topological domaini852 – 89746Lumenal By similarityAdd
BLAST
Transmembranei898 – 91720Helical; Name=8; By similarityAdd
BLAST
Topological domaini918 – 93013Cytoplasmic By similarityAdd
BLAST
Transmembranei931 – 94919Helical; Name=9; By similarityAdd
BLAST
Topological domaini950 – 96415Lumenal By similarityAdd
BLAST
Transmembranei965 – 98521Helical; Name=10; By similarityAdd
BLAST
Topological domaini986 – 106176Cytoplasmic By similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10611061Sarcoplasmic/endoplasmic reticulum calcium ATPase 3PRO_0000046205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei662 – 6621Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP18596.
PRIDEiP18596.

PTM databases

PhosphoSiteiP18596.

Expressioni

Tissue specificityi

Found in most tissues. Most abundant in large and small intestine, spleen and lung. Also detected in PC12 cells.2 Publications

Inductioni

Isoform SERCA3BC is down-regulated in all tissues except pancreas in a rat hypertension model.1 Publication

Gene expression databases

GenevestigatoriP18596.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060212.

Structurei

3D structure databases

ProteinModelPortaliP18596.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265621.
HOVERGENiHBG105648.
InParanoidiP18596.
KOiK05853.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform SERCA3BC (identifier: P18596-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEEAHLLSAA DVLRRFSVTA EGGLTLEQVT DARERYGPNE LPTEEGKSLW     50
ELVVEQFEDL LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA 100
NAIVGVWQER NAESAIEALK EYEPEMGKVI RSDRKGVQRI RARDIVPGDI 150
VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL TGESVSVTKH TDAIPDPRAV 200
NQDKKNMLFS GTNIASGKAL GVAVATGLHT ELGKIRSQMA AVEPERTPLQ 250
RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 300
ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS 350
DKTGTLTTNQ MSVCRMFVVA EAEAGACRLH EFTISGTTYT PEGEVRQGEQ 400
LVRCGQFDGL VELATICALC NDSALDYNEA KGVYEKVGEA TETALTCLVE 450
KMNVFDTDLK GLSRVERAGA CNSVIKQLMQ KEFTLEFSRD RKSMSVYCTP 500
TRADPKAQGS KMFVKGAPES VIERCSSVRV GSRTVPLSAT SREHILAKIR 550
DWGSGSHTLR CLALATRDTP PRKEDMQLDD CSQFVQYETG LTFVGCVGML 600
DPPRPEVAAC ITRCSRAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVLG 650
KAYTGREFDD LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM 700
TGDGVNDAPA LKKAEIGIAM GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG 750
RAIYNNMKQF IRYLISSNVG EVVCIFLTAI LGLPEALIPV QLLWVNLVTD 800
GLPATALGFN PPDLDIMEKL PRNPREALIS GWLFFRYLAI GVYVGLATVA 850
AATWWFLYDA EGPQVTFHQL RNFLKCSEDN PLFAGIDCEV FESRFPTTMA 900
LSVLVTIEMC NALNSVSENQ SLLRMPPWLN PWLLGAVVMS MALHFLILLV 950
PPLPLIFQVT PLSGRQWGVV LQMSLPVILL DEALKYLSRH HVDGVLETFM 1000
QAWCKQPLPG PHTTRGWLPG CHFNGWEQTE EFVFIQERWT VSGLGPEKKA 1050
RERLGLVSAA S 1061
Length:1,061
Mass (Da):116,284
Last modified:February 22, 2012 - v2
Checksum:iB907C7FA73750D14
GO
Isoform SERCA3A (identifier: P18596-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     994-1061: GVLETFMQAWCKQPLPGPHTTRGWLPGCHFNGWEQTEEFVFIQERWTVSGLGPEKKARERLGLVSAAS → EKKDLK

Show »
Length:999
Mass (Da):109,359
Checksum:i8A6AEE29021AA379
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei994 – 106168GVLET…VSAAS → EKKDLK in isoform SERCA3A. VSP_042297Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30581 mRNA. Translation: AAA42131.1.
AF458230 mRNA. Translation: AAL78969.1.
PIRiA34307.
RefSeqiNP_037046.1. NM_012914.1. [P18596-2]
UniGeneiRn.9920.

Genome annotation databases

GeneIDi25391.
KEGGirno:25391.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30581 mRNA. Translation: AAA42131.1 .
AF458230 mRNA. Translation: AAL78969.1 .
PIRi A34307.
RefSeqi NP_037046.1. NM_012914.1. [P18596-2 ]
UniGenei Rn.9920.

3D structure databases

ProteinModelPortali P18596.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000060212.

PTM databases

PhosphoSitei P18596.

Proteomic databases

PaxDbi P18596.
PRIDEi P18596.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25391.
KEGGi rno:25391.

Organism-specific databases

CTDi 489.
RGDi 2175. Atp2a3.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265621.
HOVERGENi HBG105648.
InParanoidi P18596.
KOi K05853.

Miscellaneous databases

NextBioi 606461.
PROi P18596.

Gene expression databases

Genevestigatori P18596.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning, functional expression, and mRNA tissue distribution of a third organellar Ca2+ pump."
    Burk S.E., Lytton J., McLennan D.H., Shull G.E.
    J. Biol. Chem. 264:18561-18568(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SERCA3A), CHARACTERIZATION, TISSUE SPECIFICITY.
    Tissue: Kidney.
  2. "Platelet Ca(2+)ATPases: a plural, species-specific, and multiple hypertension-regulated expression system."
    Martin V., Bredoux R., Corvazier E., Papp B., Enouf J.
    Hypertension 35:91-102(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 869-1061 (ISOFORM SERCA3BC), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: Wistar Kyoto.

Entry informationi

Entry nameiAT2A3_RAT
AccessioniPrimary (citable) accession number: P18596
Secondary accession number(s): Q8R5I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 22, 2012
Last modified: May 14, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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