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P18596 (AT2A3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Short name=SERCA3
Short name=SR Ca(2+)-ATPase 3
EC=3.6.3.8
Alternative name(s):
Calcium pump 3
Gene names
Name:Atp2a3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1061 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Ref.2

Catalytic activity

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Nucleus membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Found in most tissues. Most abundant in large and small intestine, spleen and lung. Also detected in PC12 cells. Ref.1 Ref.2

Induction

Isoform SERCA3BC is down-regulated in all tissues except pancreas in a rat hypertension model. Ref.2

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform SERCA3BC (identifier: P18596-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform SERCA3A (identifier: P18596-2)

The sequence of this isoform differs from the canonical sequence as follows:
     994-1061: GVLETFMQAWCKQPLPGPHTTRGWLPGCHFNGWEQTEEFVFIQERWTVSGLGPEKKARERLGLVSAAS → EKKDLK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10611061Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
PRO_0000046205

Regions

Topological domain1 – 4848Cytoplasmic By similarity
Transmembrane49 – 6921Helical; Name=1; By similarity
Topological domain70 – 8920Lumenal By similarity
Transmembrane90 – 11021Helical; Name=2; By similarity
Topological domain111 – 253143Cytoplasmic By similarity
Transmembrane254 – 27320Helical; Name=3; By similarity
Topological domain274 – 29522Lumenal By similarity
Transmembrane296 – 31318Helical; Name=4; By similarity
Topological domain314 – 757444Cytoplasmic By similarity
Transmembrane758 – 77720Helical; Name=5; By similarity
Topological domain778 – 78710Lumenal By similarity
Transmembrane788 – 80821Helical; Name=6; By similarity
Topological domain809 – 82820Cytoplasmic By similarity
Transmembrane829 – 85123Helical; Name=7; By similarity
Topological domain852 – 89746Lumenal By similarity
Transmembrane898 – 91720Helical; Name=8; By similarity
Topological domain918 – 93013Cytoplasmic By similarity
Transmembrane931 – 94919Helical; Name=9; By similarity
Topological domain950 – 96415Lumenal By similarity
Transmembrane965 – 98521Helical; Name=10; By similarity
Topological domain986 – 106176Cytoplasmic By similarity

Sites

Active site35114-aspartylphosphate intermediate By similarity
Metal binding3041Calcium 2; via carbonyl oxygen By similarity
Metal binding3051Calcium 2; via carbonyl oxygen By similarity
Metal binding3071Calcium 2; via carbonyl oxygen By similarity
Metal binding3091Calcium 2 By similarity
Metal binding7031Magnesium By similarity
Metal binding7071Magnesium By similarity
Metal binding7681Calcium 1 By similarity
Metal binding7711Calcium 1 By similarity
Metal binding7961Calcium 2 By similarity
Metal binding7991Calcium 1 By similarity
Metal binding8001Calcium 1 By similarity
Metal binding8001Calcium 2 By similarity
Metal binding9081Calcium 1 By similarity
Binding site5151ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue6621Phosphoserine By similarity

Natural variations

Alternative sequence994 – 106168GVLET…VSAAS → EKKDLK in isoform SERCA3A.
VSP_042297

Sequences

Sequence LengthMass (Da)Tools
Isoform SERCA3BC [UniParc].

Last modified February 22, 2012. Version 2.
Checksum: B907C7FA73750D14

FASTA1,061116,284
        10         20         30         40         50         60 
MEEAHLLSAA DVLRRFSVTA EGGLTLEQVT DARERYGPNE LPTEEGKSLW ELVVEQFEDL 

        70         80         90        100        110        120 
LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA NAIVGVWQER NAESAIEALK 

       130        140        150        160        170        180 
EYEPEMGKVI RSDRKGVQRI RARDIVPGDI VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL 

       190        200        210        220        230        240 
TGESVSVTKH TDAIPDPRAV NQDKKNMLFS GTNIASGKAL GVAVATGLHT ELGKIRSQMA 

       250        260        270        280        290        300 
AVEPERTPLQ RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 

       310        320        330        340        350        360 
ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 

       370        380        390        400        410        420 
MSVCRMFVVA EAEAGACRLH EFTISGTTYT PEGEVRQGEQ LVRCGQFDGL VELATICALC 

       430        440        450        460        470        480 
NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTDLK GLSRVERAGA CNSVIKQLMQ 

       490        500        510        520        530        540 
KEFTLEFSRD RKSMSVYCTP TRADPKAQGS KMFVKGAPES VIERCSSVRV GSRTVPLSAT 

       550        560        570        580        590        600 
SREHILAKIR DWGSGSHTLR CLALATRDTP PRKEDMQLDD CSQFVQYETG LTFVGCVGML 

       610        620        630        640        650        660 
DPPRPEVAAC ITRCSRAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVLG KAYTGREFDD 

       670        680        690        700        710        720 
LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM TGDGVNDAPA LKKAEIGIAM 

       730        740        750        760        770        780 
GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG RAIYNNMKQF IRYLISSNVG EVVCIFLTAI 

       790        800        810        820        830        840 
LGLPEALIPV QLLWVNLVTD GLPATALGFN PPDLDIMEKL PRNPREALIS GWLFFRYLAI 

       850        860        870        880        890        900 
GVYVGLATVA AATWWFLYDA EGPQVTFHQL RNFLKCSEDN PLFAGIDCEV FESRFPTTMA 

       910        920        930        940        950        960 
LSVLVTIEMC NALNSVSENQ SLLRMPPWLN PWLLGAVVMS MALHFLILLV PPLPLIFQVT 

       970        980        990       1000       1010       1020 
PLSGRQWGVV LQMSLPVILL DEALKYLSRH HVDGVLETFM QAWCKQPLPG PHTTRGWLPG 

      1030       1040       1050       1060 
CHFNGWEQTE EFVFIQERWT VSGLGPEKKA RERLGLVSAA S 

« Hide

Isoform SERCA3A [UniParc].

Checksum: 8A6AEE29021AA379
Show »

FASTA999109,359

References

[1]"cDNA cloning, functional expression, and mRNA tissue distribution of a third organellar Ca2+ pump."
Burk S.E., Lytton J., McLennan D.H., Shull G.E.
J. Biol. Chem. 264:18561-18568(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SERCA3A), CHARACTERIZATION, TISSUE SPECIFICITY.
Tissue: Kidney.
[2]"Platelet Ca(2+)ATPases: a plural, species-specific, and multiple hypertension-regulated expression system."
Martin V., Bredoux R., Corvazier E., Papp B., Enouf J.
Hypertension 35:91-102(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 869-1061 (ISOFORM SERCA3BC), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Strain: Wistar Kyoto.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30581 mRNA. Translation: AAA42131.1.
AF458230 mRNA. Translation: AAL78969.1.
PIRA34307.
RefSeqNP_037046.1. NM_012914.1.
UniGeneRn.9920.

3D structure databases

ProteinModelPortalP18596.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000060212.

PTM databases

PhosphoSiteP18596.

Proteomic databases

PaxDbP18596.
PRIDEP18596.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25391.
KEGGrno:25391.

Organism-specific databases

CTD489.
RGD2175. Atp2a3.

Phylogenomic databases

eggNOGCOG0474.
HOGENOMHOG000265621.
HOVERGENHBG105648.
InParanoidP18596.
KOK05853.

Gene expression databases

GenevestigatorP18596.

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR005782. ATPase_P-typ_Ca-transp_IIA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio606461.
PROP18596.

Entry information

Entry nameAT2A3_RAT
AccessionPrimary (citable) accession number: P18596
Secondary accession number(s): Q8R5I9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 22, 2012
Last modified: February 19, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families