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Reviewed, UniProtKB/Swiss-Prot P18596 (AT2A3_RAT)

Last modified October 13, 2009. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
      Short name=SERCA3
    EC=3.6.3.8
Alternative name(s):
    Calcium pump 3
    SR Ca(2+)-ATPase 3
Gene names
Name: Atp2a3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length999 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Catalytic activity

ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Nucleus membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Found in most tissues. Most abundant in large and small intestine, spleen and lung. Ref.1

Sequence similarities

Belongs to the cation transport ATPase (P-type) family. Type IIA subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 999999Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
PRO_0000046205

Regions

Topological domain1 – 4848Cytoplasmic By similarity
Transmembrane49 – 69211 By similarity
Topological domain70 – 8920Lumenal By similarity
Transmembrane90 – 110212 By similarity
Topological domain111 – 253143Cytoplasmic By similarity
Transmembrane254 – 273203 By similarity
Topological domain274 – 29522Lumenal By similarity
Transmembrane296 – 313184 By similarity
Topological domain314 – 757444Cytoplasmic By similarity
Transmembrane758 – 777205 By similarity
Topological domain778 – 78710Lumenal By similarity
Transmembrane788 – 808216 By similarity
Topological domain809 – 82820Cytoplasmic By similarity
Transmembrane829 – 851237 By similarity
Topological domain852 – 89746Lumenal By similarity
Transmembrane898 – 917208 By similarity
Topological domain918 – 93013Cytoplasmic By similarity
Transmembrane931 – 949199 By similarity
Topological domain950 – 96415Lumenal By similarity
Transmembrane965 – 9852110 By similarity
Topological domain986 – 99914Cytoplasmic By similarity

Sites

Active site35114-aspartylphosphate intermediate By similarity
Metal binding3041Calcium 2; via carbonyl oxygen By similarity
Metal binding3051Calcium 2; via carbonyl oxygen By similarity
Metal binding3071Calcium 2; via carbonyl oxygen By similarity
Metal binding3091Calcium 2 By similarity
Metal binding7031Magnesium By similarity
Metal binding7071Magnesium By similarity
Metal binding7681Calcium 1 By similarity
Metal binding7711Calcium 1 By similarity
Metal binding7961Calcium 2 By similarity
Metal binding7991Calcium 1 By similarity
Metal binding8001Calcium 1 By similarity
Metal binding8001Calcium 2 By similarity
Metal binding9081Calcium 1 By similarity
Binding site5151ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

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Sequences

Sequence LengthMass (Da)Tools
P18596-1 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 8A6AEE29021AA379

FASTA999109,359
        10         20         30         40         50         60 
MEEAHLLSAA DVLRRFSVTA EGGLTLEQVT DARERYGPNE LPTEEGKSLW ELVVEQFEDL 

        70         80         90        100        110        120 
LVRILLLAAL VSFVLAWFEE GEETTTAFVE PLVIMLILVA NAIVGVWQER NAESAIEALK 

       130        140        150        160        170        180 
EYEPEMGKVI RSDRKGVQRI RARDIVPGDI VEVAVGDKVP ADLRLIEIKS TTLRVDQSIL 

       190        200        210        220        230        240 
TGESVSVTKH TDAIPDPRAV NQDKKNMLFS GTNIASGKAL GVAVATGLHT ELGKIRSQMA 

       250        260        270        280        290        300 
AVEPERTPLQ RKLDEFGRQL SHAISVICVA VWVINIGHFA DPAHGGSWLR GAVYYFKIAV 

       310        320        330        340        350        360 
ALAVAAIPEG LPAVITTCLA LGTRRMARKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 

       370        380        390        400        410        420 
MSVCRMFVVA EAEAGACRLH EFTISGTTYT PEGEVRQGEQ LVRCGQFDGL VELATICALC 

       430        440        450        460        470        480 
NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTDLK GLSRVERAGA CNSVIKQLMQ 

       490        500        510        520        530        540 
KEFTLEFSRD RKSMSVYCTP TRADPKAQGS KMFVKGAPES VIERCSSVRV GSRTVPLSAT 

       550        560        570        580        590        600 
SREHILAKIR DWGSGSHTLR CLALATRDTP PRKEDMQLDD CSQFVQYETG LTFVGCVGML 

       610        620        630        640        650        660 
DPPRPEVAAC ITRCSRAGIR VVMITGDNKG TAVAICRRLG IFGDTEDVLG KAYTGREFDD 

       670        680        690        700        710        720 
LSPEQQRQAC RTARCFARVE PAHKSRIVEN LQSFNEITAM TGDGVNDAPA LKKAEIGIAM 

       730        740        750        760        770        780 
GSGTAVAKSA AEMVLSDDNF ASIVAAVEEG RAIYNNMKQF IRYLISSNVG EVVCIFLTAI 

       790        800        810        820        830        840 
LGLPEALIPV QLLWVNLVTD GLPATALGFN PPDLDIMEKL PRNPREALIS GWLFFRYLAI 

       850        860        870        880        890        900 
GVYVGLATVA AATWWFLYDA EGPQVTFHQL RNFLKCSEDN PLFAGIDCEV FESRFPTTMA 

       910        920        930        940        950        960 
LSVLVTIEMC NALNSVSENQ SLLRMPPWLN PWLLGAVVMS MALHFLILLV PPLPLIFQVT 

       970        980        990 
PLSGRQWGVV LQMSLPVILL DEALKYLSRH HVDEKKDLK

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References

[1]"cDNA cloning, functional expression, and mRNA tissue distribution of a third organellar Ca2+ pump."
Burk S.E., Lytton J., McLennan D.H., Shull G.E.
J. Biol. Chem. 264:18561-18568(1989) [PubMed: 2553713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY.
Tissue: Kidney.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M30581 mRNA. Translation: AAA42131.1.
IPIIPI00192914.
PIRA34307.
RefSeqNP_037046.1.
UniGeneRn.9920

3D structure databases

HSSPHSSP built from PDB template 1EUL based on UniProtKB P04191.
SMRP18596. Positions 1-993.
ModBaseSearch...

Protein-protein interaction databases

STRINGP18596.

PTM databases

PhosphoSiteP18596.

Proteomic databases

PRIDEP18596.

Genome annotation databases

EnsemblENSRNOT00000024318; ENSRNOP00000024318; ENSRNOG00000017912; Rattus norvegicus. [Genome view]
GeneID25391.
KEGGrno:25391.
UCSCNM_012914. rat.

Organism-specific databases

CTD25391.
RGD2175. Atp2a3.

Phylogenomic databases

HOVERGENP18596.

Enzyme and pathway databases

BRENDA3.6.3.8. 248.

Gene expression databases

ArrayExpressP18596.
GenevestigatorP18596.
GermOnlineENSRNOG00000017912. Rattus norvegicus.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-reg.
IPR005782. ATPase_P-typ_Ca-transp.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]
PANTHERPTHR11939. ATPase_P. 1 hit.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
TIGRFAMsTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 4 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio606461.

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Entry information

Entry nameAT2A3_RAT
AccessionPrimary (citable) accession number: P18596
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: October 13, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents