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Protein

Cationic amino acid transporter 2

Gene

Slc7a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1 functions as low-affinity, high capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine). Isoform 2 also functions as permease that mediates the transport of the cationic amino acids (arginine, lysine and ornithine), but it has much higher affinity for arginine than isoform 1. May play a role in classical or alternative activation of macrophages via its role in arginine transport.3 Publications

GO - Molecular functioni

  • antiporter activity Source: GO_Central
  • arginine transmembrane transporter activity Source: MGI
  • high-affinity arginine transmembrane transporter activity Source: MGI
  • high-affinity L-ornithine transmembrane transporter activity Source: MGI
  • high-affinity lysine transmembrane transporter activity Source: MGI
  • L-arginine transmembrane transporter activity Source: MGI
  • L-lysine transmembrane transporter activity Source: MGI
  • L-ornithine transmembrane transporter activity Source: MGI
  • low-affinity L-arginine transmembrane transporter activity Source: MGI

GO - Biological processi

  • arginine transport Source: Ensembl
  • L-arginine import across plasma membrane Source: MGI
  • L-arginine transport Source: MGI
  • L-lysine import across plasma membrane Source: MGI
  • L-ornithine import across plasma membrane Source: MGI
  • L-ornithine transmembrane transport Source: MGI
  • macrophage activation Source: MGI
  • nitric oxide biosynthetic process Source: MGI
  • nitric oxide production involved in inflammatory response Source: MGI
  • regulation of inflammatory response Source: MGI
  • regulation of macrophage activation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-352230. Amino acid transport across the plasma membrane.

Protein family/group databases

TCDBi2.A.3.3.2. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cationic amino acid transporter 2
Short name:
CAT-2
Short name:
CAT2
Alternative name(s):
20.5
Low affinity cationic amino acid transporter 21 Publication
Solute carrier family 7 member 2
T-cell early activation protein1 Publication
Short name:
TEA1 Publication
Gene namesi
Name:Slc7a2
Synonyms:Atrc2, Tea
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:99828. Slc7a2.

Subcellular locationi

  • Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 59HelicalSequence analysisAdd BLAST22
Topological domaini60 – 63ExtracellularSequence analysis4
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Topological domaini85 – 104CytoplasmicSequence analysisAdd BLAST20
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 163ExtracellularSequence analysisAdd BLAST38
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Topological domaini185 – 192CytoplasmicSequence analysis8
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Topological domaini214 – 248ExtracellularSequence analysisAdd BLAST35
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Topological domaini270 – 289CytoplasmicSequence analysisAdd BLAST20
Transmembranei290 – 309HelicalSequence analysisAdd BLAST20
Topological domaini310 – 339ExtracellularSequence analysisAdd BLAST30
Transmembranei340 – 360HelicalSequence analysisAdd BLAST21
Topological domaini361 – 385CytoplasmicSequence analysisAdd BLAST25
Transmembranei386 – 406HelicalSequence analysisAdd BLAST21
Topological domaini407 – 409ExtracellularSequence analysis3
Transmembranei410 – 430HelicalSequence analysisAdd BLAST21
Topological domaini431 – 489CytoplasmicSequence analysisAdd BLAST59
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 523ExtracellularSequence analysisAdd BLAST13
Transmembranei524 – 548HelicalSequence analysisAdd BLAST25
Topological domaini549 – 556CytoplasmicSequence analysis8
Transmembranei557 – 577HelicalSequence analysisAdd BLAST21
Topological domaini578 – 581ExtracellularSequence analysis4
Transmembranei582 – 602HelicalSequence analysisAdd BLAST21
Topological domaini603 – 657CytoplasmicSequence analysisAdd BLAST55

GO - Cellular componenti

  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542651 – 657Cationic amino acid transporter 2Add BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Glycosylationi157N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1
Modified residuei463PhosphoserineBy similarity1
Modified residuei645PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP18581.
PaxDbiP18581.
PRIDEiP18581.

PTM databases

iPTMnetiP18581.
PhosphoSitePlusiP18581.

Expressioni

Tissue specificityi

Detected in liver (at protein level) (PubMed:8385111). Highest expression in liver and T-cells. Also expressed in brain and lung.2 Publications

Inductioni

By macrophage activation.1 Publication

Gene expression databases

BgeeiENSMUSG00000031596.
ExpressionAtlasiP18581. baseline and differential.
GenevisibleiP18581. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096414.

Structurei

3D structure databases

ProteinModelPortaliP18581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1286. Eukaryota.
COG0531. LUCA.
GeneTreeiENSGT00760000119151.
HOGENOMiHOG000250623.
HOVERGENiHBG000280.
InParanoidiP18581.
KOiK13864.
TreeFamiTF315212.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004755. Cat_AA_permease.
IPR029485. CAT_C.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 3 hits.
PfamiPF13520. AA_permease_2. 1 hit.
PF13906. AA_permease_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00906. 2A0303. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P18581-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIPCRAVLTF ARCLIRRKIV TLDSLEDSKL CRCLTTVDLI ALGVGSTLGA
60 70 80 90 100
GVYVLAGEVA KADSGPSIVV SFLIAALASV MAGLCYAEFG ARVPKTGSAY
110 120 130 140 150
LYTYVTVGEL WAFITGWNLI LSYVIGTSSV ARAWSGTFDE LLNKQIGQFF
160 170 180 190 200
KTYFKMNYTG LAEYPDFFAV CLVLLLAGLL SFGVKESAWV NKFFTAINIL
210 220 230 240 250
VLLFVMVAGF VKGNVANWKI SEEFLKNISA SAREPPSENG TSIYGAGGFM
260 270 280 290 300
PYGFTGTLAG AATCFYAFVG FDCIATTGEE VRNPQKAIPI GIVTSLLVCF
310 320 330 340 350
MAYFGVSAAL TLMMPYYLLD EKSPLPVAFE YVRWSPAKYV VSAGSLCALS
360 370 380 390 400
TSLLGSMFPL PRILFAMARD GLLFRFLARV SKRQSPVAAT MTAGVISAVM
410 420 430 440 450
AFLFDLKALV DMMSIGTLMA YSLVAACVLI LRYQPGLCYD QPKYTPEKET
460 470 480 490 500
LESCTNATLK SESQVTMLQG QGFSLRTLFS PSALPTRQSA SLVSFLVGFL
510 520 530 540 550
AFLILGLSIL TTYGVQAIAR LEAWSLALLA LFLVLCVAVI LTIWRQPQNQ
560 570 580 590 600
QKVAFMVPFL PFLPAFSILV NIYLMVQLSA DTWIRFSIWM ALGFLIYFAY
610 620 630 640 650
GIRHSLEGNP RDEEDDEDAF SDNINAATEE KSAMQANDHH QRNLSLPFIL

HEKTSEC
Length:657
Mass (Da):71,856
Last modified:October 3, 2012 - v3
Checksum:i140CC99D3AB96082
GO
Isoform 2 (identifier: P18581-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     357-397: MFPLPRILFA...AATMTAGVIS → IFPMPRVIYA...IATLSSGAVA

Note: Affinity of isoform 2 for arginine uptake is 70-fold higher than for isoform 1.1 Publication
Show »
Length:658
Mass (Da):71,786
Checksum:i4DF5DF9EE57BB7EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142L → P in BAE32720 (PubMed:16141072).Curated1
Sequence conflicti242S → R in BAE32720 (PubMed:16141072).Curated1
Sequence conflicti280E → K in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti335S → G in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti335S → G in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti335S → G in AAA37350 (PubMed:8385111).Curated1
Sequence conflicti335S → G in AAA20397 (PubMed:8195186).Curated1
Sequence conflicti335S → G in AAY87029 (PubMed:16239143).Curated1
Sequence conflicti335S → G in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti335S → G in AAI27083 (PubMed:15489334).Curated1
Sequence conflicti342S → A in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti342S → A in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti342S → A in AAA37350 (PubMed:8385111).Curated1
Sequence conflicti342S → A in AAA20397 (PubMed:8195186).Curated1
Sequence conflicti342S → A in AAY87029 (PubMed:16239143).Curated1
Sequence conflicti342S → A in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti342S → A in AAI27083 (PubMed:15489334).Curated1
Sequence conflicti357M → I in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti357M → I in BAE32720 (PubMed:16141072).Curated1
Sequence conflicti357M → I in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti369R → E in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti369R → E in BAE32720 (PubMed:16141072).Curated1
Sequence conflicti369R → E in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti430I → M in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti440D → E in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti440D → E in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti440D → E in AAA37350 (PubMed:8385111).Curated1
Sequence conflicti440D → E in AAA20397 (PubMed:8195186).Curated1
Sequence conflicti440D → E in AAY87029 (PubMed:16239143).Curated1
Sequence conflicti440D → E in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti440D → E in AAI27083 (PubMed:15489334).Curated1
Sequence conflicti537V → A in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti537V → A in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti537V → A in AAA37350 (PubMed:8385111).Curated1
Sequence conflicti537V → A in AAA20397 (PubMed:8195186).Curated1
Sequence conflicti537V → A in AAY87029 (PubMed:16239143).Curated1
Sequence conflicti537V → A in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti537V → A in AAI27083 (PubMed:15489334).Curated1
Sequence conflicti622D → E in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti622D → E in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti622D → E in AAA37350 (PubMed:8385111).Curated1
Sequence conflicti622D → E in AAA20397 (PubMed:8195186).Curated1
Sequence conflicti622D → E in AAY87029 (PubMed:16239143).Curated1
Sequence conflicti622D → E in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti622D → E in AAI27083 (PubMed:15489334).Curated1
Sequence conflicti626A → V in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti626A → V in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti626A → V in AAA37350 (PubMed:8385111).Curated1
Sequence conflicti626A → V in AAA20397 (PubMed:8195186).Curated1
Sequence conflicti626A → V in AAY87029 (PubMed:16239143).Curated1
Sequence conflicti626A → V in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti626A → V in AAI27083 (PubMed:15489334).Curated1
Sequence conflicti633A → V in AAA75250 (PubMed:1694015).Curated1
Sequence conflicti633A → V in AAA37372 (PubMed:8385111).Curated1
Sequence conflicti633A → V in AAA37350 (PubMed:8385111).Curated1
Sequence conflicti633A → V in AAA20397 (PubMed:8195186).Curated1
Sequence conflicti633A → V in AAY87029 (PubMed:16239143).Curated1
Sequence conflicti633A → V in BAE42415 (PubMed:16141072).Curated1
Sequence conflicti633A → V in AAI27083 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000025357 – 397MFPLP…AGVIS → IFPMPRVIYAMAEDGLLFKC LAQINSKTKTPVIATLSSGA VA in isoform 2. 4 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62838 mRNA. Translation: AAA75250.1.
L03290 mRNA. Translation: AAA37372.1.
L11600 mRNA. Translation: AAA37350.1.
L29006 mRNA. Translation: AAA20397.1.
DQ086834 mRNA. Translation: AAY87029.1.
AK154621 mRNA. Translation: BAE32720.1.
AK171369 mRNA. Translation: BAE42415.1.
AC116511 Genomic DNA. No translation available.
BC127082 mRNA. Translation: AAI27083.1.
CCDSiCCDS22258.1. [P18581-1]
CCDS40327.1. [P18581-2]
PIRiA54011.
RefSeqiNP_001038205.1. NM_001044740.2. [P18581-2]
NP_031540.2. NM_007514.3. [P18581-1]
XP_006509316.1. XM_006509253.3. [P18581-2]
UniGeneiMm.4676.

Genome annotation databases

EnsembliENSMUST00000057784; ENSMUSP00000058866; ENSMUSG00000031596. [P18581-1]
ENSMUST00000098816; ENSMUSP00000096414; ENSMUSG00000031596. [P18581-2]
ENSMUST00000117077; ENSMUSP00000113729; ENSMUSG00000031596. [P18581-2]
GeneIDi11988.
KEGGimmu:11988.
UCSCiuc009lnf.1. mouse. [P18581-1]
uc009lng.1. mouse. [P18581-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62838 mRNA. Translation: AAA75250.1.
L03290 mRNA. Translation: AAA37372.1.
L11600 mRNA. Translation: AAA37350.1.
L29006 mRNA. Translation: AAA20397.1.
DQ086834 mRNA. Translation: AAY87029.1.
AK154621 mRNA. Translation: BAE32720.1.
AK171369 mRNA. Translation: BAE42415.1.
AC116511 Genomic DNA. No translation available.
BC127082 mRNA. Translation: AAI27083.1.
CCDSiCCDS22258.1. [P18581-1]
CCDS40327.1. [P18581-2]
PIRiA54011.
RefSeqiNP_001038205.1. NM_001044740.2. [P18581-2]
NP_031540.2. NM_007514.3. [P18581-1]
XP_006509316.1. XM_006509253.3. [P18581-2]
UniGeneiMm.4676.

3D structure databases

ProteinModelPortaliP18581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096414.

Protein family/group databases

TCDBi2.A.3.3.2. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP18581.
PhosphoSitePlusiP18581.

Proteomic databases

MaxQBiP18581.
PaxDbiP18581.
PRIDEiP18581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057784; ENSMUSP00000058866; ENSMUSG00000031596. [P18581-1]
ENSMUST00000098816; ENSMUSP00000096414; ENSMUSG00000031596. [P18581-2]
ENSMUST00000117077; ENSMUSP00000113729; ENSMUSG00000031596. [P18581-2]
GeneIDi11988.
KEGGimmu:11988.
UCSCiuc009lnf.1. mouse. [P18581-1]
uc009lng.1. mouse. [P18581-2]

Organism-specific databases

CTDi6542.
MGIiMGI:99828. Slc7a2.

Phylogenomic databases

eggNOGiKOG1286. Eukaryota.
COG0531. LUCA.
GeneTreeiENSGT00760000119151.
HOGENOMiHOG000250623.
HOVERGENiHBG000280.
InParanoidiP18581.
KOiK13864.
TreeFamiTF315212.

Enzyme and pathway databases

ReactomeiR-MMU-352230. Amino acid transport across the plasma membrane.

Miscellaneous databases

ChiTaRSiSlc7a2. mouse.
PROiP18581.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031596.
ExpressionAtlasiP18581. baseline and differential.
GenevisibleiP18581. MM.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004755. Cat_AA_permease.
IPR029485. CAT_C.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 3 hits.
PfamiPF13520. AA_permease_2. 1 hit.
PF13906. AA_permease_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00906. 2A0303. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCTR2_MOUSE
AccessioniPrimary (citable) accession number: P18581
Secondary accession number(s): E9QPL9
, Q38RA6, Q3TB99, Q3U3R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.