P18566 (RBS2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain A, chloroplastic
Short name=RuBisCO small subunit A
EC=4.1.1.39

Gene names

Name:	RBCS-A
Ordered Locus Names:	Os12g0291100, LOC_Os12g19381, LOC_Os12g19394

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW response to blue light Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cold Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to far red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast thylakoid membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene cytosolic ribosome Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid lumen Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	copper ion binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 47	47	Chloroplast By similarity
	Chain	48 – 175	128	Ribulose bisphosphate carboxylase small chain A, chloroplastic		PRO_0000031538
Regions
	Region	60 – 64	5	Interaction with large subunit By similarity
Experimental info
	Sequence conflict	47	1	C → F in BAA00539. Ref.1
	Sequence conflict	145	1	V → I in BAA00539. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P18566 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: BB1F731484324EF9	FASTA	175	19,631
	10 20 30 40 50 60 MAPTVMASSA TSVAPFQGLK STAGLPVSRR STNSGFGNVS NGGRIKCMQV WPIEGIKKFE 70 80 90 100 110 120 TLSYLPPLTV EDLLKQIEYL LRSKWVPCLE FSKVGFVYRE NHRSPGYYDG RYWTMWKLPM 130 140 150 160 170 FGCTDATQVL KELEEAKKAY PDAFVRIIGF DNVRQVQLIS FIAYKPPGCE ESGGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Classification and nucleotide sequence of cDNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase from rice." Matsuoka M., Kano-Murakami Y., Tanaka Y., Ozeki Y., Yamamoto N. Plant Cell Physiol. 29:1015-1022(1988) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Cloning and characterization of a full-length cDNA encoding the RuBPCase small subunit (rbcS) in rice (Oryza sativa L.)." Xiang T. J. Zhejiang Univ. Sci. Ed. 32:314-319(2005) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[6]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00644 mRNA. Translation: BAA00539.1. AY445627 mRNA. Translation: AAR19268.1. DP000011 Genomic DNA. Translation: ABA97169.1. AP008218 Genomic DNA. Translation: BAF29623.1. CM000149 Genomic DNA. Translation: EEE53069.1.
PIR	RKRZS6. JU0005.
RefSeq	NP_001066604.1. NM_001073136.1.
UniGene	Os.20927.
3D structure databases
ProteinModelPortal	P18566.
SMR	P18566. Positions 49-169.
ModBase	Search...
Proteomic databases
PaxDb	P18566.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os12g19381.1; LOC_Os12g19381.1; LOC_Os12g19381.
GeneID	4352014.
KEGG	osa:4352014.
Organism-specific databases
Gramene	P18566.
Phylogenomic databases
eggNOG	COG4451.
HOGENOM	HOG000141332.
KO	K01602.
OMA	APTVMAS.
ProtClustDB	PLN02289.
Gene expression databases
ArrayExpress	P18566.
Family and domain databases
Gene3D	3.30.190.10. 1 hit.
InterPro	IPR024681. RuBisCO_sc. IPR000894. RuBisCO_sc_dom. IPR024680. RuBisCO_ssu_N. [Graphical view]
Pfam	PF12338. RbcS. 1 hit. PF00101. RuBisCO_small. 1 hit. [Graphical view]
PRINTS	PR00152. RUBISCOSMALL.
SUPFAM	SSF55239. RuBisCO_small. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RBS2_ORYSJ

Accession

Primary (citable) accession number: P18566
Secondary accession number(s): Q0INU2, Q6SYB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	June 27, 2006
Last modified:	April 3, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program