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P18562

- UPP_YEAST

UniProt

P18562 - UPP_YEAST

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Protein

Uracil phosphoribosyltransferase

Gene

FUR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.2 Publications

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP By similarity.By similarity

Enzyme regulationi

Allosterically activated by GTP.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411GTPBy similarity
Binding sitei77 – 771GTPBy similarity
Binding sitei85 – 8515-phospho-alpha-D-ribose 1-diphosphateBy similarity
Binding sitei102 – 1021GTPBy similarity
Binding sitei110 – 11015-phospho-alpha-D-ribose 1-diphosphateBy similarity
Binding sitei131 – 1311GTPBy similarity
Binding sitei137 – 13715-phospho-alpha-D-ribose 1-diphosphateBy similarity
Binding sitei201 – 2011Ribose-5-phosphateBy similarity
Binding sitei202 – 2021Uracil; via amide nitrogenBy similarity
Binding sitei208 – 20815-phospho-alpha-D-ribose 1-diphosphateBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. uracil phosphoribosyltransferase activity Source: SGD

GO - Biological processi

  1. pyrimidine-containing compound salvage Source: SGD
  2. UMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YHR128W-MONOMER.
YEAST:YHR128W-MONOMER.
UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferase (EC:2.4.2.9)
Short name:
UPRTase
Alternative name(s):
UMP pyrophosphorylase
Gene namesi
Name:FUR1
Ordered Locus Names:YHR128W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR128w.
SGDiS000001170. FUR1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi26 – 261R → S in FUR1-8; causes resistance to 5-fluorouracil (5FU). 1 Publication
Mutagenesisi99 – 991R → S in FUR1-5; causes resistance to 5-fluorouracil (5FU). 1 Publication
Mutagenesisi106 – 1061I → N in FUR1-7; causes resistance to 5-fluorouracil (5FU). 1 Publication
Mutagenesisi173 – 1731E → G in FUR1-9; causes resistance to 5-fluorouracil (5FU). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Uracil phosphoribosyltransferasePRO_0000120788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei82 – 821Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18562.
PaxDbiP18562.
PeptideAtlasiP18562.

Expressioni

Inductioni

Induced by uracil.1 Publication

Gene expression databases

GenevestigatoriP18562.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
URK1P275154EBI-20122,EBI-20151

Protein-protein interaction databases

BioGridi36561. 22 interactions.
DIPiDIP-1323N.
IntActiP18562. 16 interactions.
MINTiMINT-377162.
STRINGi4932.YHR128W.

Structurei

3D structure databases

ProteinModelPortaliP18562.
SMRiP18562. Positions 6-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 14595-phospho-alpha-D-ribose 1-diphosphate bindingBy similarity
Regioni207 – 2093Uracil bindingBy similarity

Sequence similaritiesi

Belongs to the UPRTase family.Curated

Phylogenomic databases

eggNOGiCOG0035.
GeneTreeiENSGT00510000047272.
HOGENOMiHOG000262755.
InParanoidiP18562.
KOiK00761.
OMAiPKLFYEK.
OrthoDBiEOG7BKD5H.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR029057. PRTase-like.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.

Sequencei

Sequence statusi: Complete.

P18562-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSEPFKNVY LLPQTNQLLG LYTIIRNKNT TRPDFIFYSD RIIRLLVEEG
60 70 80 90 100
LNHLPVQKQI VETDTNENFE GVSFMGKICG VSIVRAGESM EQGLRDCCRS
110 120 130 140 150
VRIGKILIQR DEETALPKLF YEKLPEDISE RYVFLLDPML ATGGSAIMAT
160 170 180 190 200
EVLIKRGVKP ERIYFLNLIC SKEGIEKYHA AFPEVRIVTG ALDRGLDENK
210
YLVPGLGDFG DRYYCV
Length:216
Mass (Da):24,594
Last modified:September 5, 2006 - v2
Checksum:iE02179D26A53732A
GO

Sequence cautioni

The sequence AAA34611.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAB19947.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAB68405.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAT93101.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence CAA56207.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041Missing in AAB19947. (PubMed:1913872)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36485 Genomic DNA. Translation: AAA34611.1. Different initiation.
S57516 Genomic DNA. Translation: AAB19947.2. Different initiation.
X79811 Genomic DNA. Translation: CAA56207.1. Different initiation.
U10398 Genomic DNA. Translation: AAB68405.1. Different initiation.
AY693082 Genomic DNA. Translation: AAT93101.1. Different initiation.
BK006934 Genomic DNA. Translation: DAA06821.1.
PIRiJH0147.
RefSeqiNP_011996.2. NM_001179258.1.

Genome annotation databases

EnsemblFungiiYHR128W; YHR128W; YHR128W.
GeneIDi856529.
KEGGisce:YHR128W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36485 Genomic DNA. Translation: AAA34611.1 . Different initiation.
S57516 Genomic DNA. Translation: AAB19947.2 . Different initiation.
X79811 Genomic DNA. Translation: CAA56207.1 . Different initiation.
U10398 Genomic DNA. Translation: AAB68405.1 . Different initiation.
AY693082 Genomic DNA. Translation: AAT93101.1 . Different initiation.
BK006934 Genomic DNA. Translation: DAA06821.1 .
PIRi JH0147.
RefSeqi NP_011996.2. NM_001179258.1.

3D structure databases

ProteinModelPortali P18562.
SMRi P18562. Positions 6-214.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36561. 22 interactions.
DIPi DIP-1323N.
IntActi P18562. 16 interactions.
MINTi MINT-377162.
STRINGi 4932.YHR128W.

Proteomic databases

MaxQBi P18562.
PaxDbi P18562.
PeptideAtlasi P18562.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR128W ; YHR128W ; YHR128W .
GeneIDi 856529.
KEGGi sce:YHR128W.

Organism-specific databases

CYGDi YHR128w.
SGDi S000001170. FUR1.

Phylogenomic databases

eggNOGi COG0035.
GeneTreei ENSGT00510000047272.
HOGENOMi HOG000262755.
InParanoidi P18562.
KOi K00761.
OMAi PKLFYEK.
OrthoDBi EOG7BKD5H.

Enzyme and pathway databases

UniPathwayi UPA00574 ; UER00636 .
BioCyci MetaCyc:YHR128W-MONOMER.
YEAST:YHR128W-MONOMER.

Miscellaneous databases

NextBioi 982301.
PROi P18562.

Gene expression databases

Genevestigatori P18562.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR029057. PRTase-like.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The FUR1 gene of Saccharomyces cerevisiae: cloning, structure and expression of wild-type and mutant alleles."
    Kern L., de Montigny J., Jund R., Lacroute F.
    Gene 88:149-157(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ARG-26; ILE-106 AND GLU-173.
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "Regulation of the pyrimidine salvage pathway by the FUR1 gene product of Saccharomyces cerevisiae."
    Kern L., de Montigny J., Lacroute F., Jund R.
    Curr. Genet. 19:333-337(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ARG-99, INDUCTION.
  3. "ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle."
    Clark S.W., Meyer D.I.
    J. Cell Biol. 127:129-138(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26109 / X2180 / NCYC 826.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUPP_YEAST
AccessioniPrimary (citable) accession number: P18562
Secondary accession number(s): D3DL77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3