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P18555

- KITH_ASFB7

UniProt

P18555 - KITH_ASFB7

Protein

Thymidine kinase

Gene

Ba71V-050

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine By similarity.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Proton acceptorSequence Analysis
    Binding sitei121 – 1211Substrate; via amide nitrogenBy similarity
    Metal bindingi146 – 1461ZincBy similarity
    Metal bindingi149 – 1491ZincBy similarity
    Metal bindingi179 – 1791ZincBy similarity
    Metal bindingi182 – 1821ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 248ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis, Virulence

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Short name:
    TDK
    Gene namesi
    Ordered Locus Names:Ba71V-050
    ORF Names:K196R
    OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
    Taxonomic identifieri10498 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
    Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000624: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196Thymidine kinasePRO_0000174927Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP18555.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1705Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18555-1 [UniParc]FASTAAdd to Basket

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    MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT    50
    RDKTIKTHSG IQLRPKQCKI IESTQLSDVG SLTDIHAVVV DEAHFFDDLI 100
    KCRTWAEEEK IIILAGLNAS FEQKMFPPIV RIFPYCSWVK YIGRTCMKCN 150
    QHNACFNVRK NADKTLILAG GSELYVTCCN NCLKNTFIKQ LQPIKY 196
    Length:196
    Mass (Da):22,421
    Last modified:November 1, 1990 - v1
    Checksum:i94AA5C64C98985A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21490 Genomic DNA. Translation: CAG33722.1.
    M63119 Genomic DNA. Translation: AAA42737.1.
    U18466 Genomic DNA. Translation: AAA65280.1.
    PIRiA37079. KIXFAS.
    RefSeqiNP_042744.1. NC_001659.1.

    Genome annotation databases

    GeneIDi1488815.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21490 Genomic DNA. Translation: CAG33722.1 .
    M63119 Genomic DNA. Translation: AAA42737.1 .
    U18466 Genomic DNA. Translation: AAA65280.1 .
    PIRi A37079. KIXFAS.
    RefSeqi NP_042744.1. NC_001659.1.

    3D structure databases

    ProteinModelPortali P18555.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1488815.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and evolutionary relationships of African swine fever virus thymidine kinase."
      Blasco R., Lopez-Otin C., Munoz M., Bockamp E.-O., Simon-Mateo C., Vinuela E.
      Virology 178:301-304(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Expression and characterization of the thymidine kinase gene of African swine fever virus."
      Hernandez A.M., Taberes E.
      J. Virol. 65:1046-1052(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Analysis of the complete nucleotide sequence of African swine fever virus."
      Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
      Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiKITH_ASFB7
    AccessioniPrimary (citable) accession number: P18555
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3