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P18555 (KITH_ASFB7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

Short name=TDK
EC=2.7.1.21
Gene names
Ordered Locus Names:Ba71V-050
ORF Names:K196R
OrganismAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) [Reference proteome]
Taxonomic identifier10498 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine By similarity.

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sequence similarities

Belongs to the thymidine kinase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 196196Thymidine kinase
PRO_0000174927

Regions

Nucleotide binding17 – 248ATP Probable
Region166 – 1705Substrate binding By similarity

Sites

Active site921Proton acceptor Potential
Metal binding1461Zinc By similarity
Metal binding1491Zinc By similarity
Metal binding1791Zinc By similarity
Metal binding1821Zinc By similarity
Binding site1211Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P18555 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 94AA5C64C98985A0

FASTA19622,421
        10         20         30         40         50         60 
MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT RDKTIKTHSG 

        70         80         90        100        110        120 
IQLRPKQCKI IESTQLSDVG SLTDIHAVVV DEAHFFDDLI KCRTWAEEEK IIILAGLNAS 

       130        140        150        160        170        180 
FEQKMFPPIV RIFPYCSWVK YIGRTCMKCN QHNACFNVRK NADKTLILAG GSELYVTCCN 

       190 
NCLKNTFIKQ LQPIKY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and evolutionary relationships of African swine fever virus thymidine kinase."
Blasco R., Lopez-Otin C., Munoz M., Bockamp E.-O., Simon-Mateo C., Vinuela E.
Virology 178:301-304(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Expression and characterization of the thymidine kinase gene of African swine fever virus."
Hernandez A.M., Taberes E.
J. Virol. 65:1046-1052(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Analysis of the complete nucleotide sequence of African swine fever virus."
Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z21490 Genomic DNA. Translation: CAG33722.1.
M63119 Genomic DNA. Translation: AAA42737.1.
U18466 Genomic DNA. Translation: AAA65280.1.
PIRKIXFAS. A37079.
RefSeqNP_042744.1. NC_001659.1.

3D structure databases

ProteinModelPortalP18555.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1488815.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKITH_ASFB7
AccessionPrimary (citable) accession number: P18555
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families