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Protein

Thymidine kinase

Gene

Ba71V-050

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Proton acceptorSequence Analysis
Binding sitei121 – 1211Substrate; via amide nitrogenBy similarity
Metal bindingi146 – 1461ZincBy similarity
Metal bindingi149 – 1491ZincBy similarity
Metal bindingi179 – 1791ZincBy similarity
Metal bindingi182 – 1821ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 248ATPCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis, Virulence

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Short name:
TDK
Gene namesi
Ordered Locus Names:Ba71V-050
ORF Names:K196R
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000624 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Thymidine kinasePRO_0000174927Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP18555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 1705Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

KOiK00857.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT
60 70 80 90 100
RDKTIKTHSG IQLRPKQCKI IESTQLSDVG SLTDIHAVVV DEAHFFDDLI
110 120 130 140 150
KCRTWAEEEK IIILAGLNAS FEQKMFPPIV RIFPYCSWVK YIGRTCMKCN
160 170 180 190
QHNACFNVRK NADKTLILAG GSELYVTCCN NCLKNTFIKQ LQPIKY
Length:196
Mass (Da):22,421
Last modified:November 1, 1990 - v1
Checksum:i94AA5C64C98985A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21490 Genomic DNA. Translation: CAG33722.1.
M63119 Genomic DNA. Translation: AAA42737.1.
U18466 Genomic DNA. Translation: AAA65280.1.
PIRiA37079. KIXFAS.
RefSeqiNP_042744.1. NC_001659.2.

Genome annotation databases

GeneIDi22220432.
KEGGivg:22220432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21490 Genomic DNA. Translation: CAG33722.1.
M63119 Genomic DNA. Translation: AAA42737.1.
U18466 Genomic DNA. Translation: AAA65280.1.
PIRiA37079. KIXFAS.
RefSeqiNP_042744.1. NC_001659.2.

3D structure databases

ProteinModelPortaliP18555.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22220432.
KEGGivg:22220432.

Phylogenomic databases

KOiK00857.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and evolutionary relationships of African swine fever virus thymidine kinase."
    Blasco R., Lopez-Otin C., Munoz M., Bockamp E.-O., Simon-Mateo C., Vinuela E.
    Virology 178:301-304(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Expression and characterization of the thymidine kinase gene of African swine fever virus."
    Hernandez A.M., Taberes E.
    J. Virol. 65:1046-1052(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of the complete nucleotide sequence of African swine fever virus."
    Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
    Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKITH_ASFB7
AccessioniPrimary (citable) accession number: P18555
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 1, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.