P18555 (KITH_ASFB7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
Short name=TDK
EC=2.7.1.21

Gene names

Ordered Locus Names:	Ba71V-050
ORF Names:	K196R

Organism

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) [Reference proteome]

Taxonomic identifier

10498 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus › African swine fever virus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine By similarity.
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis Virulence
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Thymidine kinase		PRO_0000174927
Regions
	Nucleotide binding	17 – 24	8	ATP Probable
	Region	166 – 170	5	Substrate binding By similarity
Sites
	Active site	92	1	Proton acceptor Potential
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	182	1	Zinc By similarity
	Binding site	121	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18555 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 94AA5C64C98985A0	FASTA	196	22,421
	10 20 30 40 50 60 MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT RDKTIKTHSG 70 80 90 100 110 120 IQLRPKQCKI IESTQLSDVG SLTDIHAVVV DEAHFFDDLI KCRTWAEEEK IIILAGLNAS 130 140 150 160 170 180 FEQKMFPPIV RIFPYCSWVK YIGRTCMKCN QHNACFNVRK NADKTLILAG GSELYVTCCN 190 NCLKNTFIKQ LQPIKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and evolutionary relationships of African swine fever virus thymidine kinase." Blasco R., Lopez-Otin C., Munoz M., Bockamp E.-O., Simon-Mateo C., Vinuela E. Virology 178:301-304(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Expression and characterization of the thymidine kinase gene of African swine fever virus." Hernandez A.M., Taberes E. J. Virol. 65:1046-1052(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of the complete nucleotide sequence of African swine fever virus." Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E. Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21490 Genomic DNA. Translation: CAG33722.1. M63119 Genomic DNA. Translation: AAA42737.1. U18466 Genomic DNA. Translation: AAA65280.1.
PIR	KIXFAS. A37079.
RefSeq	NP_042744.1. NC_001659.1.
3D structure databases
ProteinModelPortal	P18555.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1488815.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_ASFB7

Accession

Primary (citable) accession number: P18555

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program