P18549 (CEFD_STRC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isopenicillin N epimerase
EC=5.1.1.17

Gene names

Name:

cefD

Organism

Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)

Taxonomic identifier

443255 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces clavuligerus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible isomerization between isopenicillin N and penicillin N.
Catalytic activity	Isopenicillin N = penicillin N.
Cofactor	Pyridoxal phosphate.
Pathway	Antibiotic biosynthesis; cephalosporin C biosynthesis.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	isopenicillin-N epimerase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 398	397	Isopenicillin N epimerase		PRO_0000150241
Amino acid modifications
	Modified residue	217	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18549 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EDE15C08246DA77A	FASTA	398	43,498
	10 20 30 40 50 60 MAVADWEEAR GRMLLDPTVV NLNTGSGGPL PRSAFERVTG FRAHLAAEPM DFLLREVPAL 70 80 90 100 110 120 LWQARESLAR LIGGDPLRLA LATNVTAAVN LVASSLRLEA PGEILLSDDE YTPMRWCWER 130 140 150 160 170 180 VARRHGLELR TFRLPELPSD PAEITAAAVA AMGPRTRLFF FSHVVSTTGL ILPAAELCEE 190 200 210 220 230 240 ARARGITTVV DGAHAPGFLD LDLSRIPCDF YAGSGHKWLL APTGVGFLHL APGRLEELEP 250 260 270 280 290 300 TQVSWAYEPP EGSGPPAARD RFGSTPGLRR LECEGTRDIC PWLATPESID FQAELGPGAI 310 320 330 340 350 360 RARRRELTDH ARRLLADRPG RTLLTPDSPE LSGGMVAYRL PPGTDAAELR RGLWERFRIE 370 380 390 AAVAEQPPGP VLRISANFYT TEEEIDRLAD ALDALTGE

References

[1]

"The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus." Kovacevic S., Tobin M.B., Miller J.R. J. Bacteriol. 172:3952-3958(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

[2]

"Purification and properties of isopenicillin N epimerase from Streptomyces clavuligerus." Usui S., Yu C.-A. Biochim. Biophys. Acta 999:78-85(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32324 Genomic DNA. Translation: AAA26714.1.
PIR	T52311.
3D structure databases
ProteinModelPortal	P18549.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13381.
UniPathway	UPA00172.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CEFD_STRC2

Accession

Primary (citable) accession number: P18549

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program