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P18548

- CEFE_STRC2

UniProt

P18548 - CEFE_STRC2

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Protein
Deacetoxycephalosporin C synthase
Gene
cefE
Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.

Catalytic activityi

Penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O.

Cofactori

Iron.
Ascorbate.

Pathwayi

GO - Molecular functioni

  1. L-ascorbic acid binding Source: UniProtKB-KW
  2. deacetoxycephalosporin-C synthase activity Source: UniProtKB-EC
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Vitamin C

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13407.
BRENDAi1.14.20.1. 5988.
SABIO-RKP18548.
UniPathwayiUPA00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Deacetoxycephalosporin C synthase (EC:1.14.20.1)
Short name:
DAOCS
Alternative name(s):
Expandase
Gene namesi
Name:cefE
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Deacetoxycephalosporin C synthase
PRO_0000219511Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93
Helixi10 – 145
Turni15 – 184
Helixi19 – 2810
Beta strandi31 – 388
Helixi41 – 5717
Helixi60 – 656
Beta strandi73 – 797
Helixi94 – 963
Beta strandi99 – 1035
Beta strandi105 – 1073
Helixi113 – 14028
Helixi149 – 1535
Beta strandi158 – 1647
Beta strandi180 – 1856
Beta strandi187 – 1959
Beta strandi204 – 2085
Beta strandi211 – 2144
Beta strandi222 – 2265
Helixi228 – 2336
Turni234 – 2363
Beta strandi243 – 2453
Helixi250 – 2523
Beta strandi258 – 2658
Beta strandi272 – 2743
Helixi275 – 2806
Beta strandi289 – 2935
Helixi294 – 2985
Beta strandi305 – 3073

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCSX-ray1.30A1-311[»]
1E5HX-ray1.96A1-308[»]
1E5IX-ray2.10A1-306[»]
1HJFX-ray1.60A1-311[»]
1HJGX-ray1.50A1-311[»]
1RXFX-ray1.50A1-311[»]
1RXGX-ray1.50A1-311[»]
1UNBX-ray1.50A1-311[»]
1UO9X-ray1.50A1-311[»]
1UOBX-ray1.70A1-311[»]
1UOFX-ray1.60A1-311[»]
1UOGX-ray1.70A1-311[»]
1W28X-ray2.30A1-311[»]
1W2AX-ray2.51X1-311[»]
1W2NX-ray2.70A1-311[»]
1W2OX-ray3.00A1-311[»]
2JB8X-ray1.53A1-311[»]
ProteinModelPortaliP18548.
SMRiP18548. Positions 1-308.

Miscellaneous databases

EvolutionaryTraceiP18548.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 267114Fe2OG dioxygenase
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18548-1 [UniParc]FASTAAdd to Basket

« Hide

MDTTVPTFSL AELQQGLHQD EFRRCLRDKG LFYLTDCGLT DTELKSAKDL    50
VIDFFEHGSE AEKRAVTSPV PTMRRGFTGL ESESTAQITN TGSYSDYSMC 100
YSMGTADNLF PSGDFERIWT QYFDRQYTAS RAVAREVLRA TGTEPDGGVE 150
AFLDCEPLLR FRYFPQVPEH RSAEEQPLRM APHYDLSMVT LIQQTPCANG 200
FVSLQAEVGG AFTDLPYRPD AVLVFCGAIA TLVTGGQVKA PRHHVAAPRR 250
DQIAGSSRTS SVFFLRPNAD FTFSVPLARE CGFDVSLDGE TATFQDWIGG 300
NYVNIRRTSK A 311
Length:311
Mass (Da):34,556
Last modified:November 1, 1990 - v1
Checksum:i9C64E1FC37F524BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32324 Genomic DNA. Translation: AAA26715.1.
PIRiA32043.
T52312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32324 Genomic DNA. Translation: AAA26715.1 .
PIRi A32043.
T52312.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DCS X-ray 1.30 A 1-311 [» ]
1E5H X-ray 1.96 A 1-308 [» ]
1E5I X-ray 2.10 A 1-306 [» ]
1HJF X-ray 1.60 A 1-311 [» ]
1HJG X-ray 1.50 A 1-311 [» ]
1RXF X-ray 1.50 A 1-311 [» ]
1RXG X-ray 1.50 A 1-311 [» ]
1UNB X-ray 1.50 A 1-311 [» ]
1UO9 X-ray 1.50 A 1-311 [» ]
1UOB X-ray 1.70 A 1-311 [» ]
1UOF X-ray 1.60 A 1-311 [» ]
1UOG X-ray 1.70 A 1-311 [» ]
1W28 X-ray 2.30 A 1-311 [» ]
1W2A X-ray 2.51 X 1-311 [» ]
1W2N X-ray 2.70 A 1-311 [» ]
1W2O X-ray 3.00 A 1-311 [» ]
2JB8 X-ray 1.53 A 1-311 [» ]
ProteinModelPortali P18548.
SMRi P18548. Positions 1-308.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00172 .
BioCyci MetaCyc:MONOMER-13407.
BRENDAi 1.14.20.1. 5988.
SABIO-RK P18548.

Miscellaneous databases

EvolutionaryTracei P18548.

Family and domain databases

Gene3Di 2.60.120.330. 1 hit.
InterProi IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view ]
Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view ]
PROSITEi PS51471. FE2OG_OXY. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase."
    Kovacevic S., Weigel B.J., Tobin M.B., Ingolia T.D., Miller J.R.
    J. Bacteriol. 171:754-760(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  2. "The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus."
    Kovacevic S., Tobin M.B., Miller J.R.
    J. Bacteriol. 172:3952-3958(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Entry informationi

Entry nameiCEFE_STRC2
AccessioniPrimary (citable) accession number: P18548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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