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P18548 (CEFE_STRC2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deacetoxycephalosporin C synthase

Short name=DAOCS
EC=1.14.20.1
Alternative name(s):
Expandase
Gene names
Name:cefE
OrganismStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifier443255 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.

Catalytic activity

Penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O.

Cofactor

Iron.

Ascorbate.

Pathway

Antibiotic biosynthesis; cephalosporin C biosynthesis.

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family.

Contains 1 Fe2OG dioxygenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Deacetoxycephalosporin C synthase
PRO_0000219511

Regions

Domain154 – 267114Fe2OG dioxygenase

Secondary structure

..................................................... 311
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18548 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 9C64E1FC37F524BC

FASTA31134,556
        10         20         30         40         50         60 
MDTTVPTFSL AELQQGLHQD EFRRCLRDKG LFYLTDCGLT DTELKSAKDL VIDFFEHGSE 

        70         80         90        100        110        120 
AEKRAVTSPV PTMRRGFTGL ESESTAQITN TGSYSDYSMC YSMGTADNLF PSGDFERIWT 

       130        140        150        160        170        180 
QYFDRQYTAS RAVAREVLRA TGTEPDGGVE AFLDCEPLLR FRYFPQVPEH RSAEEQPLRM 

       190        200        210        220        230        240 
APHYDLSMVT LIQQTPCANG FVSLQAEVGG AFTDLPYRPD AVLVFCGAIA TLVTGGQVKA 

       250        260        270        280        290        300 
PRHHVAAPRR DQIAGSSRTS SVFFLRPNAD FTFSVPLARE CGFDVSLDGE TATFQDWIGG 

       310 
NYVNIRRTSK A 

« Hide

References

[1]"Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase."
Kovacevic S., Weigel B.J., Tobin M.B., Ingolia T.D., Miller J.R.
J. Bacteriol. 171:754-760(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
[2]"The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus."
Kovacevic S., Tobin M.B., Miller J.R.
J. Bacteriol. 172:3952-3958(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32324 Genomic DNA. Translation: AAA26715.1.
PIRA32043.
T52312.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCSX-ray1.30A1-311[»]
1E5HX-ray1.96A1-308[»]
1E5IX-ray2.10A1-306[»]
1HJFX-ray1.60A1-311[»]
1HJGX-ray1.50A1-311[»]
1RXFX-ray1.50A1-311[»]
1RXGX-ray1.50A1-311[»]
1UNBX-ray1.50A1-311[»]
1UO9X-ray1.50A1-311[»]
1UOBX-ray1.70A1-311[»]
1UOFX-ray1.60A1-311[»]
1UOGX-ray1.70A1-311[»]
1W28X-ray2.30A1-311[»]
1W2AX-ray2.51X1-311[»]
1W2NX-ray2.70A1-311[»]
1W2OX-ray3.00A1-311[»]
2JB8X-ray1.53A1-311[»]
ProteinModelPortalP18548.
SMRP18548. Positions 1-308.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13407.
BRENDA1.14.20.1. 5988.
SABIO-RKP18548.
UniPathwayUPA00172.

Family and domain databases

Gene3D2.60.120.330. 1 hit.
InterProIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP18548.

Entry information

Entry nameCEFE_STRC2
AccessionPrimary (citable) accession number: P18548
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways