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P18548

- CEFE_STRC2

UniProt

P18548 - CEFE_STRC2

Protein

Deacetoxycephalosporin C synthase

Gene

cefE

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.

    Catalytic activityi

    Penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O.

    Cofactori

    Iron.
    Ascorbate.

    Pathwayi

    GO - Molecular functioni

    1. deacetoxycephalosporin-C synthase activity Source: UniProtKB-EC
    2. iron ion binding Source: InterPro
    3. L-ascorbic acid binding Source: UniProtKB-KW
    4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Source: InterPro

    GO - Biological processi

    1. antibiotic biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Keywords - Ligandi

    Iron, Vitamin C

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13407.
    BRENDAi1.14.20.1. 5988.
    SABIO-RKP18548.
    UniPathwayiUPA00172.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deacetoxycephalosporin C synthase (EC:1.14.20.1)
    Short name:
    DAOCS
    Alternative name(s):
    Expandase
    Gene namesi
    Name:cefE
    OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
    Taxonomic identifieri443255 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Deacetoxycephalosporin C synthasePRO_0000219511Add
    BLAST

    Structurei

    Secondary structure

    1
    311
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 93
    Helixi10 – 145
    Turni15 – 184
    Helixi19 – 2810
    Beta strandi31 – 388
    Helixi41 – 5717
    Helixi60 – 656
    Beta strandi73 – 797
    Helixi94 – 963
    Beta strandi99 – 1035
    Beta strandi105 – 1073
    Helixi113 – 14028
    Helixi149 – 1535
    Beta strandi158 – 1647
    Beta strandi180 – 1856
    Beta strandi187 – 1959
    Beta strandi204 – 2085
    Beta strandi211 – 2144
    Beta strandi222 – 2265
    Helixi228 – 2336
    Turni234 – 2363
    Beta strandi243 – 2453
    Helixi250 – 2523
    Beta strandi258 – 2658
    Beta strandi272 – 2743
    Helixi275 – 2806
    Beta strandi289 – 2935
    Helixi294 – 2985
    Beta strandi305 – 3073

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DCSX-ray1.30A1-311[»]
    1E5HX-ray1.96A1-308[»]
    1E5IX-ray2.10A1-306[»]
    1HJFX-ray1.60A1-311[»]
    1HJGX-ray1.50A1-311[»]
    1RXFX-ray1.50A1-311[»]
    1RXGX-ray1.50A1-311[»]
    1UNBX-ray1.50A1-311[»]
    1UO9X-ray1.50A1-311[»]
    1UOBX-ray1.70A1-311[»]
    1UOFX-ray1.60A1-311[»]
    1UOGX-ray1.70A1-311[»]
    1W28X-ray2.30A1-311[»]
    1W2AX-ray2.51X1-311[»]
    1W2NX-ray2.70A1-311[»]
    1W2OX-ray3.00A1-311[»]
    2JB8X-ray1.53A1-311[»]
    ProteinModelPortaliP18548.
    SMRiP18548. Positions 1-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18548.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini154 – 267114Fe2OG dioxygenasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.60.120.330. 1 hit.
    InterProiIPR026992. DIOX_N.
    IPR027443. IPNS-like.
    IPR002057. Isopenicillin-N_synth_CS.
    IPR005123. Oxoglu/Fe-dep_dioxygenase.
    [Graphical view]
    PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
    PF14226. DIOX_N. 1 hit.
    [Graphical view]
    PROSITEiPS51471. FE2OG_OXY. 1 hit.
    PS00186. IPNS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18548-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTTVPTFSL AELQQGLHQD EFRRCLRDKG LFYLTDCGLT DTELKSAKDL    50
    VIDFFEHGSE AEKRAVTSPV PTMRRGFTGL ESESTAQITN TGSYSDYSMC 100
    YSMGTADNLF PSGDFERIWT QYFDRQYTAS RAVAREVLRA TGTEPDGGVE 150
    AFLDCEPLLR FRYFPQVPEH RSAEEQPLRM APHYDLSMVT LIQQTPCANG 200
    FVSLQAEVGG AFTDLPYRPD AVLVFCGAIA TLVTGGQVKA PRHHVAAPRR 250
    DQIAGSSRTS SVFFLRPNAD FTFSVPLARE CGFDVSLDGE TATFQDWIGG 300
    NYVNIRRTSK A 311
    Length:311
    Mass (Da):34,556
    Last modified:November 1, 1990 - v1
    Checksum:i9C64E1FC37F524BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32324 Genomic DNA. Translation: AAA26715.1.
    PIRiA32043.
    T52312.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32324 Genomic DNA. Translation: AAA26715.1 .
    PIRi A32043.
    T52312.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DCS X-ray 1.30 A 1-311 [» ]
    1E5H X-ray 1.96 A 1-308 [» ]
    1E5I X-ray 2.10 A 1-306 [» ]
    1HJF X-ray 1.60 A 1-311 [» ]
    1HJG X-ray 1.50 A 1-311 [» ]
    1RXF X-ray 1.50 A 1-311 [» ]
    1RXG X-ray 1.50 A 1-311 [» ]
    1UNB X-ray 1.50 A 1-311 [» ]
    1UO9 X-ray 1.50 A 1-311 [» ]
    1UOB X-ray 1.70 A 1-311 [» ]
    1UOF X-ray 1.60 A 1-311 [» ]
    1UOG X-ray 1.70 A 1-311 [» ]
    1W28 X-ray 2.30 A 1-311 [» ]
    1W2A X-ray 2.51 X 1-311 [» ]
    1W2N X-ray 2.70 A 1-311 [» ]
    1W2O X-ray 3.00 A 1-311 [» ]
    2JB8 X-ray 1.53 A 1-311 [» ]
    ProteinModelPortali P18548.
    SMRi P18548. Positions 1-308.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00172 .
    BioCyci MetaCyc:MONOMER-13407.
    BRENDAi 1.14.20.1. 5988.
    SABIO-RK P18548.

    Miscellaneous databases

    EvolutionaryTracei P18548.

    Family and domain databases

    Gene3Di 2.60.120.330. 1 hit.
    InterProi IPR026992. DIOX_N.
    IPR027443. IPNS-like.
    IPR002057. Isopenicillin-N_synth_CS.
    IPR005123. Oxoglu/Fe-dep_dioxygenase.
    [Graphical view ]
    Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
    PF14226. DIOX_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51471. FE2OG_OXY. 1 hit.
    PS00186. IPNS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase."
      Kovacevic S., Weigel B.J., Tobin M.B., Ingolia T.D., Miller J.R.
      J. Bacteriol. 171:754-760(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
    2. "The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus."
      Kovacevic S., Tobin M.B., Miller J.R.
      J. Bacteriol. 172:3952-3958(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

    Entry informationi

    Entry nameiCEFE_STRC2
    AccessioniPrimary (citable) accession number: P18548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3