Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Deacetoxycephalosporin C synthase

Gene

cefE

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.

Catalytic activityi

Penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: cephalosporin C biosynthesis

This protein is involved in the pathway cephalosporin C biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cephalosporin C biosynthesis and in Antibiotic biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Vitamin C

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13407.
BRENDAi1.14.20.1. 5988.
SABIO-RKP18548.
UniPathwayiUPA00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Deacetoxycephalosporin C synthase (EC:1.14.20.1)
Short name:
DAOCS
Alternative name(s):
Expandase
Gene namesi
Name:cefE
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002195111 – 311Deacetoxycephalosporin C synthaseAdd BLAST311

Interactioni

Protein-protein interaction databases

STRINGi443255.SclaA2_010100020198.

Structurei

Secondary structure

1311
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Helixi10 – 14Combined sources5
Turni15 – 18Combined sources4
Helixi19 – 28Combined sources10
Beta strandi31 – 38Combined sources8
Helixi41 – 57Combined sources17
Helixi60 – 65Combined sources6
Beta strandi73 – 79Combined sources7
Helixi94 – 96Combined sources3
Beta strandi99 – 103Combined sources5
Beta strandi105 – 107Combined sources3
Helixi113 – 140Combined sources28
Helixi149 – 153Combined sources5
Beta strandi158 – 164Combined sources7
Beta strandi180 – 185Combined sources6
Beta strandi187 – 195Combined sources9
Beta strandi204 – 208Combined sources5
Beta strandi211 – 214Combined sources4
Beta strandi222 – 226Combined sources5
Helixi228 – 233Combined sources6
Turni234 – 236Combined sources3
Beta strandi243 – 245Combined sources3
Helixi250 – 252Combined sources3
Beta strandi258 – 265Combined sources8
Beta strandi272 – 274Combined sources3
Helixi275 – 280Combined sources6
Beta strandi289 – 293Combined sources5
Helixi294 – 298Combined sources5
Beta strandi305 – 307Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCSX-ray1.30A1-311[»]
1E5HX-ray1.96A1-308[»]
1E5IX-ray2.10A1-306[»]
1HJFX-ray1.60A1-311[»]
1HJGX-ray1.50A1-311[»]
1RXFX-ray1.50A1-311[»]
1RXGX-ray1.50A1-311[»]
1UNBX-ray1.50A1-311[»]
1UO9X-ray1.50A1-311[»]
1UOBX-ray1.70A1-311[»]
1UOFX-ray1.60A1-311[»]
1UOGX-ray1.70A1-311[»]
1W28X-ray2.30A1-311[»]
1W2AX-ray2.51X1-311[»]
1W2NX-ray2.70A1-311[»]
1W2OX-ray3.00A1-311[»]
2JB8X-ray1.53A1-311[»]
ProteinModelPortaliP18548.
SMRiP18548.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18548.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini154 – 267Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST114

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTTVPTFSL AELQQGLHQD EFRRCLRDKG LFYLTDCGLT DTELKSAKDL
60 70 80 90 100
VIDFFEHGSE AEKRAVTSPV PTMRRGFTGL ESESTAQITN TGSYSDYSMC
110 120 130 140 150
YSMGTADNLF PSGDFERIWT QYFDRQYTAS RAVAREVLRA TGTEPDGGVE
160 170 180 190 200
AFLDCEPLLR FRYFPQVPEH RSAEEQPLRM APHYDLSMVT LIQQTPCANG
210 220 230 240 250
FVSLQAEVGG AFTDLPYRPD AVLVFCGAIA TLVTGGQVKA PRHHVAAPRR
260 270 280 290 300
DQIAGSSRTS SVFFLRPNAD FTFSVPLARE CGFDVSLDGE TATFQDWIGG
310
NYVNIRRTSK A
Length:311
Mass (Da):34,556
Last modified:November 1, 1990 - v1
Checksum:i9C64E1FC37F524BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32324 Genomic DNA. Translation: AAA26715.1.
PIRiA32043.
T52312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32324 Genomic DNA. Translation: AAA26715.1.
PIRiA32043.
T52312.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCSX-ray1.30A1-311[»]
1E5HX-ray1.96A1-308[»]
1E5IX-ray2.10A1-306[»]
1HJFX-ray1.60A1-311[»]
1HJGX-ray1.50A1-311[»]
1RXFX-ray1.50A1-311[»]
1RXGX-ray1.50A1-311[»]
1UNBX-ray1.50A1-311[»]
1UO9X-ray1.50A1-311[»]
1UOBX-ray1.70A1-311[»]
1UOFX-ray1.60A1-311[»]
1UOGX-ray1.70A1-311[»]
1W28X-ray2.30A1-311[»]
1W2AX-ray2.51X1-311[»]
1W2NX-ray2.70A1-311[»]
1W2OX-ray3.00A1-311[»]
2JB8X-ray1.53A1-311[»]
ProteinModelPortaliP18548.
SMRiP18548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi443255.SclaA2_010100020198.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00172.
BioCyciMetaCyc:MONOMER-13407.
BRENDAi1.14.20.1. 5988.
SABIO-RKP18548.

Miscellaneous databases

EvolutionaryTraceiP18548.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEFE_STRC2
AccessioniPrimary (citable) accession number: P18548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.