Reviewed, UniProtKB/Swiss-Prot P18548 (CEFE_STRCL)

Names and origin

Protein names

Recommended name:
    Deacetoxycephalosporin C synthetase
      Short name=DAOCS
    EC=1.14.20.1
Alternative name(s):
    Expandase

Gene names

Name:

cefE

Organism

Streptomyces clavuligerus

Taxonomic identifier

1901 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.
Catalytic activity	Penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; cephalosporin C biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Vitamin C
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW deacetoxycephalosporin-C synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 311

311

Deacetoxycephalosporin C synthetase

PRO_0000219511

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

311

Helix Strand Turn

Details...

Beta strand

7 – 9

3

Helix

10 – 14

5

Helix

19 – 28

10

Beta strand

31 – 38

8

Helix

41 – 57

17

Helix

60 – 65

6

Beta strand

73 – 79

7

Beta strand

99 – 103

5

Beta strand

105 – 107

3

Helix

113 – 140

28

Helix

149 – 153

5

Beta strand

158 – 164

7

Beta strand

180 – 185

6

Beta strand

187 – 195

9

Beta strand

204 – 208

5

Beta strand

211 – 214

4

Beta strand

222 – 226

5

Helix

228 – 233

6

Beta strand

243 – 245

3

Beta strand

258 – 265

8

Beta strand

272 – 274

3

Helix

275 – 280

6

Beta strand

289 – 293

5

Helix

294 – 298

5

Sequences

	Sequence		Length	Mass (Da)
	P18548-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 9C64E1FC37F524BC	FASTA	311	34,556
	10 20 30 40 50 60 MDTTVPTFSL AELQQGLHQD EFRRCLRDKG LFYLTDCGLT DTELKSAKDL VIDFFEHGSE 70 80 90 100 110 120 AEKRAVTSPV PTMRRGFTGL ESESTAQITN TGSYSDYSMC YSMGTADNLF PSGDFERIWT 130 140 150 160 170 180 QYFDRQYTAS RAVAREVLRA TGTEPDGGVE AFLDCEPLLR FRYFPQVPEH RSAEEQPLRM 190 200 210 220 230 240 APHYDLSMVT LIQQTPCANG FVSLQAEVGG AFTDLPYRPD AVLVFCGAIA TLVTGGQVKA 250 260 270 280 290 300 PRHHVAAPRR DQIAGSSRTS SVFFLRPNAD FTFSVPLARE CGFDVSLDGE TATFQDWIGG 310 NYVNIRRTSK A

References

[1]	"Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase." Kovacevic S., Weigel B.J., Tobin M.B., Ingolia T.D., Miller J.R. J. Bacteriol. 171:754-760(1989) [PubMed: 2644235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27064 / DSM 738 / IFO 13307 / JCM 4710 / NRRL 3585.
[2]	"The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus." Kovacevic S., Tobin M.B., Miller J.R. J. Bacteriol. 172:3952-3958(1990) [PubMed: 1694525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27064 / DSM 738 / IFO 13307 / JCM 4710 / NRRL 3585.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M32324 Genomic DNA. Translation: AAA26715.1.

PIR

A32043.
T52312.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DCS	X-ray	1.30	A	1-311	[»]
1E5H	X-ray	1.96	A	1-307	[»]
1E5I	X-ray	2.10	A	1-306	[»]
1HJF	X-ray	1.60	A	1-311	[»]
1HJG	X-ray	1.50	A	1-311	[»]
1RXF	X-ray	1.50	A	1-311	[»]
1RXG	X-ray	1.50	A	1-311	[»]
1UNB	X-ray	1.50	A	1-311	[»]
1UO9	X-ray	1.50	A	1-311	[»]
1UOB	X-ray	1.70	A	1-311	[»]
1UOF	X-ray	1.60	A	1-311	[»]
1UOG	X-ray	1.70	A	1-311	[»]
1W28	X-ray	2.30	A	1-311	[»]
1W2A	X-ray	2.51	X	1-311	[»]
1W2N	X-ray	2.70	A	1-311	[»]
1W2O	X-ray	3.00	A	1-311	[»]
2JB8	X-ray	1.53	A	1-311	[»]

ModBase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MON-13407.

BRENDA

1.14.20.1. 229786.

Family and domain databases

InterPro

IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglutarate/Fe-dep_Oase.
[Graphical view]

Pfam

PF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]

PROSITE

PS00185. IPNS_1. False negative.
PS00186. IPNS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CEFE_STRCL

Accession

Primary (citable) accession number: P18548

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)