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P18548

- CEFE_STRC2

UniProt

P18548 - CEFE_STRC2

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Protein

Deacetoxycephalosporin C synthase

Gene

cefE

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.

Catalytic activityi

Penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

GO - Molecular functioni

  1. deacetoxycephalosporin-C synthase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro
  3. L-ascorbic acid binding Source: UniProtKB-KW
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Vitamin C

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13407.
BRENDAi1.14.20.1. 5988.
SABIO-RKP18548.
UniPathwayiUPA00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Deacetoxycephalosporin C synthase (EC:1.14.20.1)
Short name:
DAOCS
Alternative name(s):
Expandase
Gene namesi
Name:cefE
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Deacetoxycephalosporin C synthasePRO_0000219511Add
BLAST

Structurei

Secondary structure

1
311
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi10 – 145Combined sources
Turni15 – 184Combined sources
Helixi19 – 2810Combined sources
Beta strandi31 – 388Combined sources
Helixi41 – 5717Combined sources
Helixi60 – 656Combined sources
Beta strandi73 – 797Combined sources
Helixi94 – 963Combined sources
Beta strandi99 – 1035Combined sources
Beta strandi105 – 1073Combined sources
Helixi113 – 14028Combined sources
Helixi149 – 1535Combined sources
Beta strandi158 – 1647Combined sources
Beta strandi180 – 1856Combined sources
Beta strandi187 – 1959Combined sources
Beta strandi204 – 2085Combined sources
Beta strandi211 – 2144Combined sources
Beta strandi222 – 2265Combined sources
Helixi228 – 2336Combined sources
Turni234 – 2363Combined sources
Beta strandi243 – 2453Combined sources
Helixi250 – 2523Combined sources
Beta strandi258 – 2658Combined sources
Beta strandi272 – 2743Combined sources
Helixi275 – 2806Combined sources
Beta strandi289 – 2935Combined sources
Helixi294 – 2985Combined sources
Beta strandi305 – 3073Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCSX-ray1.30A1-311[»]
1E5HX-ray1.96A1-308[»]
1E5IX-ray2.10A1-306[»]
1HJFX-ray1.60A1-311[»]
1HJGX-ray1.50A1-311[»]
1RXFX-ray1.50A1-311[»]
1RXGX-ray1.50A1-311[»]
1UNBX-ray1.50A1-311[»]
1UO9X-ray1.50A1-311[»]
1UOBX-ray1.70A1-311[»]
1UOFX-ray1.60A1-311[»]
1UOGX-ray1.70A1-311[»]
1W28X-ray2.30A1-311[»]
1W2AX-ray2.51X1-311[»]
1W2NX-ray2.70A1-311[»]
1W2OX-ray3.00A1-311[»]
2JB8X-ray1.53A1-311[»]
ProteinModelPortaliP18548.
SMRiP18548. Positions 1-308.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18548.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 267114Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18548-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTTVPTFSL AELQQGLHQD EFRRCLRDKG LFYLTDCGLT DTELKSAKDL
60 70 80 90 100
VIDFFEHGSE AEKRAVTSPV PTMRRGFTGL ESESTAQITN TGSYSDYSMC
110 120 130 140 150
YSMGTADNLF PSGDFERIWT QYFDRQYTAS RAVAREVLRA TGTEPDGGVE
160 170 180 190 200
AFLDCEPLLR FRYFPQVPEH RSAEEQPLRM APHYDLSMVT LIQQTPCANG
210 220 230 240 250
FVSLQAEVGG AFTDLPYRPD AVLVFCGAIA TLVTGGQVKA PRHHVAAPRR
260 270 280 290 300
DQIAGSSRTS SVFFLRPNAD FTFSVPLARE CGFDVSLDGE TATFQDWIGG
310
NYVNIRRTSK A
Length:311
Mass (Da):34,556
Last modified:November 1, 1990 - v1
Checksum:i9C64E1FC37F524BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32324 Genomic DNA. Translation: AAA26715.1.
PIRiA32043.
T52312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32324 Genomic DNA. Translation: AAA26715.1 .
PIRi A32043.
T52312.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DCS X-ray 1.30 A 1-311 [» ]
1E5H X-ray 1.96 A 1-308 [» ]
1E5I X-ray 2.10 A 1-306 [» ]
1HJF X-ray 1.60 A 1-311 [» ]
1HJG X-ray 1.50 A 1-311 [» ]
1RXF X-ray 1.50 A 1-311 [» ]
1RXG X-ray 1.50 A 1-311 [» ]
1UNB X-ray 1.50 A 1-311 [» ]
1UO9 X-ray 1.50 A 1-311 [» ]
1UOB X-ray 1.70 A 1-311 [» ]
1UOF X-ray 1.60 A 1-311 [» ]
1UOG X-ray 1.70 A 1-311 [» ]
1W28 X-ray 2.30 A 1-311 [» ]
1W2A X-ray 2.51 X 1-311 [» ]
1W2N X-ray 2.70 A 1-311 [» ]
1W2O X-ray 3.00 A 1-311 [» ]
2JB8 X-ray 1.53 A 1-311 [» ]
ProteinModelPortali P18548.
SMRi P18548. Positions 1-308.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00172 .
BioCyci MetaCyc:MONOMER-13407.
BRENDAi 1.14.20.1. 5988.
SABIO-RK P18548.

Miscellaneous databases

EvolutionaryTracei P18548.

Family and domain databases

Gene3Di 2.60.120.330. 1 hit.
InterProi IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view ]
Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view ]
PROSITEi PS51471. FE2OG_OXY. 1 hit.
PS00186. IPNS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase."
    Kovacevic S., Weigel B.J., Tobin M.B., Ingolia T.D., Miller J.R.
    J. Bacteriol. 171:754-760(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  2. "The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus."
    Kovacevic S., Tobin M.B., Miller J.R.
    J. Bacteriol. 172:3952-3958(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Entry informationi

Entry nameiCEFE_STRC2
AccessioniPrimary (citable) accession number: P18548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 26, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3