P18547 (VNCS_PAVPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-capsid protein NS-1

Alternative name(s):
Non-structural protein NS1

Gene names

Name:

NS1

Organism

Porcine parvovirus (strain NADL-2) (PPV) [Complete proteome]

Taxonomic identifier

10797 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Parvoviridae › Parvovirinae › Parvovirus

Virus host

Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Seems necessary for viral DNA replication.
Sequence similarities	Belongs to the parvoviruses non-capsid protein family. Contains 1 SF3 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Initiation of viral infection Viral penetration into host cytoplasm Viral penetration via permeabilization of host organellar membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Non-capsid protein NS-1		PRO_0000222473
Regions
	Domain	365 – 527	163	SF3 helicase
	Nucleotide binding	398 – 405	8	ATP Potential
Experimental info
	Sequence conflict	86	1	G → R in AAA46920. Ref.2
	Sequence conflict	274	1	K → R in AAA46920. Ref.2
	Sequence conflict	376	1	C → V in AAA46920. Ref.2
	Sequence conflict	376	1	C → V in AAA46916. Ref.2
	Sequence conflict	621 – 634	14	TALTQ…SNTDT → NLHLTPTPPDSAIRTP in AAA46920. Ref.2
	Sequence conflict	621 – 634	14	TALTQ…SNTDT → NLHLTPTPPDSAIRTP in AAA46916. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18547 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: C0B1DF2226A2EF0A	FASTA	660	75,300
	10 20 30 40 50 60 MAAGNTYSEE VLKATNWLQD NAQKEAFSYV FKTQKVNLNG KEIAWNNYNK DTTDAEMINL 70 80 90 100 110 120 QRGAETSWDQ ATDMEWESEI DSLTKGQVLI FDSLVKKCLF EGILQKNLSP SDCYWFIQHE 130 140 150 160 170 180 HGQDTGYHCH VLLGGKGLQQ AMGKWFRKQL NNLWSRWLIM QCKVPLTPVE RIKLRELAED 190 200 210 220 230 240 GEWVSLLTYT HKQTKKQYTK MTHFGNMIAY YFLNKKRKTT EREHGYYLSS DSGFMTNFLK 250 260 270 280 290 300 EGERHLVSHL FTEANKPETV ETTVTTAQEA KRGKIQTKKE VSIKCTIRDL VNKRCTSIED 310 320 330 340 350 360 WMMTDPDSYI EMMAQTGGEN LIKNTLEITT LTLARTKTAY DLILEKAKPS MLPTFNISNT 370 380 390 400 410 420 RTCKIFSMHN WNYIKCCHAI TCVLNRQGGK RNTILFHGPA STGKSIIAQH IANLVGNVGC 430 440 450 460 470 480 YNAANVNFPF NDCTNKNLIW IEEAGNFSNQ VNQFKAICSG QTIRIDQKGK GSKQIEPTPV 490 500 510 520 530 540 IMTTNEDITK VRIGCEERPE HTQPIRDRML NINLTRKLPG DFGLLEETEW PLICAWLVKK 550 560 570 580 590 600 GYQATMASYM HHWGNVPDWS EKWEEPKMQT PINTPTDSQI STSVKTSPAD NNYAATPIQE 610 620 630 640 650 660 DLDLALALEP WSEPTTPTFT TALTQHARFS NTDTSPTWSE IETDIRACFG ENCAPTTNLE

References

[1]	"Porcine parvovirus: DNA sequence and genome organization." Ranz A.I., Manclus J.J., Diaz-Aroca E., Casal J.I. J. Gen. Virol. 70:2541-2553(1989) [PubMed: 2794971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete nucleotide sequence of an infectious clone of porcine parvovirus, strain NADL-2." Vasudevacharya J., Basak S., Srinivas R.V., Compans R.W. Virology 178:611-616(1990) [PubMed: 2219713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide sequence analysis of the capsid genes and the right-hand terminal palindrome of porcine parvovirus, strain NADL-2." Vasudevacharya J., Basak S., Srinivas R.V., Compans R.W. Virology 173:368-377(1989) [PubMed: 2596019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 367-660.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00623 Genomic DNA. Translation: BAA00501.1. M38367 Genomic DNA. Translation: AAA46920.1. M32787 Genomic DNA. Translation: AAA46916.1.
PIR	UYPVPP. A33302.
3D structure databases
ProteinModelPortal	P18547.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014015. Helicase_SF3_DNA-vir. IPR021972. Parvovirus_NS1_C. IPR001257. Parvovirus_NS1_helicase. IPR021076. Parvovirus_NS1_N. [Graphical view]
Pfam	PF12117. DUF3580. 1 hit. PF01057. Parvo_NS1. 1 hit. PF12433. PV_NSP1. 1 hit. [Graphical view]
PROSITE	PS51206. SF3_HELICASE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VNCS_PAVPN

Accession

Primary (citable) accession number: P18547
Secondary accession number(s): P22965

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1991
Last modified:	September 21, 2011
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program