P18539 (AMPC_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase
EC=3.5.2.6

Alternative name(s):
Cephalosporinase

Gene names

Name:

ampC

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 376	355	Beta-lactamase		PRO_0000016965
Regions
	Region	328 – 330	3	Substrate binding By similarity
Sites
	Active site	79	1	Acyl-ester intermediate By similarity
	Active site	164	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18539 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 890CEE7C27925150	FASTA	376	41,096
	10 20 30 40 50 60 MTKMNRCAAL IAALILPTAH AAQQQDIDAV IQPLMKKYGV PGMAIAVSVD GKQQIYPYGV 70 80 90 100 110 120 ASKQTGKPIT EQTLFEVGSL SKTFTATLAV YAQQQSKLSF KDPASHYLPD VRGSAFDGVS 130 140 150 160 170 180 LLNLATHTSG LPLFVPDDVT NNAQLMAYYR AWQPKHPAGS YRVYSNLGIG MLGMIAAKSL 190 200 210 220 230 240 DQPFIQAMEQ GMLPALGMSH TYVQVPAAQM ANYAQGYSKD DKPVRVNPGP LDAESYGIKS 250 260 270 280 290 300 NARDLIRYLD ANLQQVKVAS VARRWPRRTS VITSAGAFTQ DLMWENYPYP VKLSRLIEGN 310 320 330 340 350 360 NAGMIMNGTP ATAITPPQPE LRAGWYNKTG STGGFSTYAV FIPAKNIAVE MLANKWFPND 370 DRVEAAYHII QALEKR

References

[1]	"Nucleotide sequence of the Serratia marcescens SR50 chromosomal ampC beta-lactamase gene." Nomura K., Yoshida T. FEMS Microbiol. Lett. 58:295-299(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sr50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52964 Genomic DNA. Translation: CAA37137.1.
PIR	QKSE. A48176.
3D structure databases
ProteinModelPortal	P18539.
ModBase	Search...
Protein family/group databases
MEROPS	S12.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P18539.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR001586. Beta-lactam_class-C_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00336. BETA_LACTAMASE_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5693.

Entry information

Entry name

AMPC_SERMA

Accession

Primary (citable) accession number: P18539

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program