Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P18511

- CO16A_CONTE

UniProt

P18511 - CO16A_CONTE

Protein

Delta-conotoxin TxVIA

Gene
N/A
Organism
Conus textile (Cloth-of-gold cone)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Delta-conotoxins bind to site 6 of voltage-gated sodium channels (Nav) and inhibit the inactivation process. Binding of this toxin is strongly calcium-dependent but not voltage-dependent. The binding site is most likely on the extracellular side of the sodium channel. Binds receptor sites on both mollusk and rat central nervous system, but despite its high affinity binding to rat sodium channel, it has no functional effect in vivo and in vitro on it. Has also no effect on Gambusia fish. Is important in mollusk for the paralysis of the prey. Upon injection of the peptide, a subordinate lobster assumes an exaggerated dominant posture (of a 'King-Kong' lobster!).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei59 – 591Susceptible to oxidation

    GO - Molecular functioni

    1. sodium channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-conotoxin TxVIA
    Short name:
    TxIA
    Alternative name(s):
    Conotoxin King-Kong 0
    Short name:
    KK-0
    OrganismiConus textile (Cloth-of-gold cone)
    Taxonomic identifieri6494 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

    Organism-specific databases

    ConoServeri598. TxVIA precursor.

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Propeptidei23 – 4927PRO_0000034900Add
    BLAST
    Peptidei52 – 7827Delta-conotoxin TxVIAPRO_0000034901Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi53 ↔ 68
    Disulfide bondi60 ↔ 72
    Disulfide bondi67 ↔ 77

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom duct.1 Publication

    Structurei

    Secondary structure

    1
    78
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi62 – 643
    Beta strandi67 – 704

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FU3NMR-A52-78[»]
    ProteinModelPortaliP18511.
    SMRiP18511. Positions 52-78.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18511.

    Family & Domainsi

    Domaini

    The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
    The cysteine framework is VI/VII (C-C-CC-C-C).

    Sequence similaritiesi

    Belongs to the conotoxin O1 superfamily.Curated

    Keywords - Domaini

    Knottin, Signal

    Family and domain databases

    InterProiIPR004214. Conotoxin.
    IPR012322. Conotoxin_d-typ_CS.
    [Graphical view]
    PfamiPF02950. Conotoxin. 1 hit.
    [Graphical view]
    PROSITEiPS60005. DELTA_CONOTOXIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18511-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLTCMMIVA VLFLTAWTFA TADDPRNGLG NLFSNAHHEM KNPEASKLNK   50
    RWCKQSGEMC NLLDQNCCDG YCIVLVCT 78
    Length:78
    Mass (Da):8,760
    Last modified:November 1, 1990 - v1
    Checksum:iC5CBFD462AD40A35
    GO

    Mass spectrometryi

    Molecular mass is 3034.2±0.05 Da from positions 52 - 78. Determined by ESI. Without oxidation at Met-59.1 Publication
    Molecular mass is 3050.189±0.05 Da from positions 52 - 78. Determined by ESI. With oxidation at Met-59.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53283 mRNA. Translation: CAA37377.1.
    PIRiS12513.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53283 mRNA. Translation: CAA37377.1 .
    PIRi S12513.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FU3 NMR - A 52-78 [» ]
    ProteinModelPortali P18511.
    SMRi P18511. Positions 52-78.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ConoServeri 598. TxVIA precursor.

    Miscellaneous databases

    EvolutionaryTracei P18511.

    Family and domain databases

    InterProi IPR004214. Conotoxin.
    IPR012322. Conotoxin_d-typ_CS.
    [Graphical view ]
    Pfami PF02950. Conotoxin. 1 hit.
    [Graphical view ]
    PROSITEi PS60005. DELTA_CONOTOXIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Constant and hypervariable regions in conotoxin propeptides."
      Woodward S.R., Cruz L.J., Olivera B.M., Hillyard D.R.
      EMBO J. 9:1015-1020(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A molluscivorous Conus toxin: conserved frameworks in conotoxins."
      Hillyard D.R., Olivera B.M., Woodward S.R., Corpuz G.P., Gray W.R., Ramilo C.A., Cruz L.J.
      Biochemistry 28:358-361(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 52-78.
    3. "Mollusc-specific toxins from the venom of Conus textile neovicarius."
      Fainzilber M., Gordon D., Hasson A., Spira M.E., Zlotkin E.
      Eur. J. Biochem. 202:589-595(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 52-78.
      Strain: Neovicarius.
    4. "Rapid sensitive analysis of cysteine rich peptide venom components."
      Ueberheide B.M., Fenyo D., Alewood P.F., Chait B.T.
      Proc. Natl. Acad. Sci. U.S.A. 106:6910-6915(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 52-78, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, DISULFIDE BONDS, OXIDATION AT MET-59.
      Tissue: Venom.
    5. "Alteration of sodium currents by new peptide toxins from the venom of a molluscivorous Conus snail."
      Hasson A., Fainzilber M., Gordon D., Zlotkin E., Spira M.E.
      Eur. J. Neurosci. 5:56-64(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "A new neurotoxin receptor site on sodium channels is identified by a conotoxin that affects sodium channel inactivation in molluscs and acts as an antagonist in rat brain."
      Fainzilber M., Kofman O., Zlotkin E., Gordon D.
      J. Biol. Chem. 269:2574-2580(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Delta-conotoxin GmVIA, a novel peptide from the venom of Conus gloriamaris."
      Shon K.-J., Hasson A., Spira M.E., Cruz L.J., Gray W.R., Olivera B.M.
      Biochemistry 33:11420-11425(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.
      Tissue: Venom.
    8. "Three-dimensional solution structure of the sodium channel agonist/antagonist delta-conotoxin TxVIA."
      Kohno T., Sasaki T., Kobayashi K., Fainzilber M., Sato K.
      J. Biol. Chem. 277:36387-36391(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

    Entry informationi

    Entry nameiCO16A_CONTE
    AccessioniPrimary (citable) accession number: P18511
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    Veratridine increases the rate of dissociation in a dose-dependent manner.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3