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Protein

Gamma-aminobutyric acid receptor subunit gamma-2

Gene

Gabrg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.1 Publication

GO - Molecular functioni

  • chloride channel activity Source: RGD
  • GABA-A receptor activity Source: RGD
  • inhibitory extracellular ligand-gated ion channel activity Source: RGD

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • chloride transmembrane transport Source: GOC
  • chloride transport Source: RGD
  • gamma-aminobutyric acid signaling pathway Source: RGD
  • ion transmembrane transport Source: GOC
  • synaptic transmission Source: Reactome
  • synaptic transmission, GABAergic Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-RNO-112314. Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell.
R-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit gamma-2
Alternative name(s):
GABA(A) receptor subunit gamma-2
Gene namesi
Name:Gabrg2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi61966. Gabrg2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini39 – 272234ExtracellularCuratedAdd
BLAST
Transmembranei273 – 29523HelicalCuratedAdd
BLAST
Transmembranei299 – 32123HelicalCuratedAdd
BLAST
Transmembranei333 – 35523HelicalCuratedAdd
BLAST
Topological domaini356 – 44287CytoplasmicCuratedAdd
BLAST
Transmembranei443 – 46523HelicalCuratedAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • cytoplasm Source: RGD
  • dendrite membrane Source: BHF-UCL
  • GABA-A receptor complex Source: UniProtKB
  • GABA receptor complex Source: BHF-UCL
  • inhibitory synapse Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907607.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3838Sequence analysisAdd
BLAST
Chaini39 – 466428Gamma-aminobutyric acid receptor subunit gamma-2PRO_0000000479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence analysis
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Disulfide bondi189 ↔ 203By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP18508.
PRIDEiP18508.

PTM databases

iPTMnetiP18508.
SwissPalmiP18508.

Expressioni

Gene expression databases

ExpressionAtlasiP18508. baseline and differential.

Interactioni

Subunit structurei

Interacts with GABARAP (By similarity). Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains.By similarity1 Publication

Protein-protein interaction databases

BioGridi248326. 1 interaction.
IntActiP18508. 3 interactions.
MINTiMINT-3371576.
STRINGi10116.ENSRNOP00000004619.

Chemistry

BindingDBiP18508.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PR9X-ray2.51P400-409[»]
ProteinModelPortaliP18508.
SMRiP18508. Positions 300-357.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18508.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP18508.
KOiK05186.
OMAiYPGFTSQ.
OrthoDBiEOG7JX342.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR005439. GABBAg2_rcpt.
IPR005437. GABBAg_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01620. GABAARGAMMA.
PR01622. GABAARGAMMA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPNTWSTG STVYSPVFSQ KMTLWILLLL SLYPGFTSQK SDDDYEDYAS
60 70 80 90 100
NKTWVLTPKV PEGDVTVILN NLLEGYDNKL RPDIGVKPTL IHTDMYVNSI
110 120 130 140 150
GPVNAINMEY TIDIFFAQTW YDRRLKFNST IKVLRLNSNM VGKIWIPDTF
160 170 180 190 200
FRNSKKADAH WITTPNRMLR IWNDGRVLYT LRLTIDAECQ LQLHNFPMDE
210 220 230 240 250
HSCPLEFSSY GYPREEIVYQ WKRSSVEVGD TRSWRLYQFS FVGLRNTTEV
260 270 280 290 300
VKTTSGDYVV MSVYFDLSRR MGYFTIQTYI PCTLIVVLSW VSFWINKDAV
310 320 330 340 350
PARTSLGITT VLTMTTLSTI ARKSLPKVSY VTAMDLFVSV CFIFVFSALV
360 370 380 390 400
EYGTLHYFVS NRKPSKDKDK KKKNPAPTID IRPRSATIQM NNATHLQERD
410 420 430 440 450
EEYGYECLDG KDCASFFCCF EDCRTGAWRH GRIHIRIAKM DSYARIFFPT
460
AFCLFNLVYW VSYLYL
Length:466
Mass (Da):54,077
Last modified:November 1, 1990 - v1
Checksum:i3C60B66E0D23072F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221M → I no nucleotide entry (PubMed:2165521).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08497 Genomic DNA. Translation: AAC42036.1.
PIRiJQ0077.
RefSeqiNP_899156.1. NM_183327.1.
UniGeneiRn.230132.

Genome annotation databases

EnsembliENSRNOT00000004619; ENSRNOP00000004619; ENSRNOG00000003241.
GeneIDi29709.
KEGGirno:29709.
UCSCiRGD:61966. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08497 Genomic DNA. Translation: AAC42036.1.
PIRiJQ0077.
RefSeqiNP_899156.1. NM_183327.1.
UniGeneiRn.230132.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PR9X-ray2.51P400-409[»]
ProteinModelPortaliP18508.
SMRiP18508. Positions 300-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248326. 1 interaction.
IntActiP18508. 3 interactions.
MINTiMINT-3371576.
STRINGi10116.ENSRNOP00000004619.

Chemistry

BindingDBiP18508.
ChEMBLiCHEMBL1907607.

PTM databases

iPTMnetiP18508.
SwissPalmiP18508.

Proteomic databases

PaxDbiP18508.
PRIDEiP18508.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004619; ENSRNOP00000004619; ENSRNOG00000003241.
GeneIDi29709.
KEGGirno:29709.
UCSCiRGD:61966. rat.

Organism-specific databases

CTDi2566.
RGDi61966. Gabrg2.

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP18508.
KOiK05186.
OMAiYPGFTSQ.
OrthoDBiEOG7JX342.

Enzyme and pathway databases

ReactomeiR-RNO-112314. Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell.
R-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Miscellaneous databases

EvolutionaryTraceiP18508.
PROiP18508.

Gene expression databases

ExpressionAtlasiP18508. baseline and differential.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR005439. GABBAg2_rcpt.
IPR005437. GABBAg_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01620. GABAARGAMMA.
PR01622. GABAARGAMMA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two novel GABAA receptor subunits exist in distinct neuronal subpopulations."
    Shivers B.D., Killisch I., Sprengel R., Sontheimer H., Koehler M., Schofield P.R., Seeburg P.H.
    Neuron 3:327-337(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
    Tissue: Brain.
  2. "Functional characteristics and sites of gene expression of the alpha 1, beta 1, gamma 2-isoform of the rat GABAA receptor."
    Malherbe P., Sigel E., Baur R., Persohn E., Richards J.G., Mohler H.
    J. Neurosci. 10:2330-2337(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGBRG2_RAT
AccessioniPrimary (citable) accession number: P18508
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This subunit carries the benzodiazepine binding site.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.