P18502 (PTC_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein patched

Alternative name(s):
Hedgehog receptor

Gene names

Name:	ptc
ORF Names:	CG2411

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1286 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Segmentation polarity protein. Acts as a receptor for the hedgehog protein (HH). Associates with the smoothened protein (SMO) to transduce the hedgehog signal leading to the activation of wingless, decapentaplegic and patched itself. Participates in cell interactions that establish pattern within the segment and the imaginal disks during development. In the absence of HH, represses the constitutive signaling activity of smo through fused (FU).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the patched family. Contains 1 SSD (sterol-sensing) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Developmental protein Receptor Segmentation polarity protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Bolwig's organ morphogenesis Inferred from mutant phenotype PubMed 10704398. Source: FlyBase anterior/posterior lineage restriction, imaginal disc Traceable author statement PubMed 10625531. Source: FlyBase axon guidance Inferred from mutant phenotype PubMed 15754211. Source: FlyBase dephosphorylation Traceable author statement PubMed 11932020. Source: FlyBase determination of genital disc primordium Inferred from mutant phenotype PubMed 15893978. Source: FlyBase embryonic pattern specification Traceable author statement PubMed 11102367. Source: FlyBase eye-antennal disc morphogenesis Inferred from mutant phenotype PubMed 11934850. Source: FlyBase germ-line stem cell division Traceable author statement PubMed 12459723. Source: FlyBase gonad development Inferred from mutant phenotype PubMed 21377458. Source: FlyBase imaginal disc-derived wing morphogenesis Inferred from mutant phenotype PubMed 16648592. Source: FlyBase lipid homeostasis Inferred from mutant phenotype PubMed 18198278. Source: FlyBase negative regulation of smoothened signaling pathway Inferred from genetic interaction PubMed 9874371. Source: FlyBase ovarian follicle cell development Traceable author statement PubMed 10822261. Source: FlyBase peripheral nervous system development Traceable author statement PubMed 11102367. Source: FlyBase proteolysis Traceable author statement PubMed 11932020. Source: FlyBase regulation of mitotic cell cycle Inferred from mutant phenotype PubMed 11279500. Source: FlyBase regulation of protein import into nucleus Inferred from mutant phenotype PubMed 14597576. Source: FlyBase segment polarity determination Inferred from electronic annotation. Source: UniProtKB-KW smoothened signaling pathway Inferred from mutant phenotype PubMed 14597576PubMed 8898207. Source: FlyBase somatic stem cell division Traceable author statement PubMed 12459723. Source: FlyBase wing disc anterior/posterior pattern formation Traceable author statement PubMed 11253649. Source: FlyBase
Cellular_component	endocytic vesicle Inferred from direct assay PubMed 18198278. Source: FlyBase integral to plasma membrane Traceable author statement PubMed 8706127. Source: FlyBase perinuclear region of cytoplasm Inferred from direct assay PubMed 8898240. Source: FlyBase
Molecular_function	hedgehog receptor activity Inferred from mutant phenotype PubMed 8898207. Source: FlyBase lipoprotein particle receptor activity Inferred from direct assay PubMed 18198278. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1286	1286	Protein patched		PRO_0000205973
Regions
	Topological domain	1 – 76	76	Cytoplasmic Potential
	Transmembrane	77 – 92	16	Helical; Potential
	Topological domain	93 – 427	335	Extracellular Potential
	Transmembrane	428 – 448	21	Helical; Potential
	Topological domain	449 – 465	17	Cytoplasmic Potential
	Transmembrane	466 – 486	21	Helical; Potential
	Topological domain	487 – 492	6	Extracellular Potential
	Transmembrane	493 – 511	19	Helical; Potential
	Topological domain	512 – 532	21	Cytoplasmic Potential
	Transmembrane	533 – 553	21	Helical; Potential
	Topological domain	554 – 562	9	Extracellular Potential
	Transmembrane	563 – 583	21	Helical; Potential
	Topological domain	584 – 677	94	Cytoplasmic Potential
	Transmembrane	678 – 699	22	Helical; Potential
	Topological domain	700 – 931	232	Extracellular Potential
	Transmembrane	932 – 952	21	Helical; Potential
	Topological domain	953 – 955	3	Cytoplasmic Potential
	Transmembrane	956 – 976	21	Helical; Potential
	Topological domain	977 – 1007	31	Extracellular Potential
	Transmembrane	1008 – 1028	21	Helical; Potential
	Topological domain	1029 – 1056	28	Cytoplasmic Potential
	Transmembrane	1057 – 1077	21	Helical; Potential
	Topological domain	1078 – 1082	5	Extracellular Potential
	Transmembrane	1083 – 1103	21	Helical; Potential
	Topological domain	1104 – 1286	183	Cytoplasmic Potential
	Domain	428 – 583	156	SSD
	Compositional bias	1227 – 1232	6	Poly-Pro
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	807	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	111	1	R → G in CAA35591. Ref.2
	Sequence conflict	274	1	G → A in CAA35591. Ref.2
	Sequence conflict	332	1	A → R in AAA28696. Ref.1
	Sequence conflict	636	1	P → A in CAA35591. Ref.2
	Sequence conflict	862 – 864	3	DVF → ASSPTELLRANCIRNR Ref.2
	Sequence conflict	866	1	Y → N Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18502 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 5F22A956F8BE0EC6	FASTA	1,286	142,831
	10 20 30 40 50 60 MDRDSLPRVP DTHGDVVDEK LFSDLYIRTS WVDAQVALDQ IDKGKARGSR TAIYLRSVFQ 70 80 90 100 110 120 SHLETLGSSV QKHAGKVLFV AILVLSTFCV GLKSAQIHSK VHQLWIQEGG RLEAELAYTQ 130 140 150 160 170 180 KTIGEDESAT HQLLIQTTHD PNASVLHPQA LLAHLEVLVK ATAVKVHLYD TEWGLRDMCN 190 200 210 220 230 240 MPSTPSFEGI YYIEQILRHL IPCSIITPLD CFWEGSQLLG PESAVVIPGL NQRLLWTTLN 250 260 270 280 290 300 PASVMQYMKQ KMSEEKISFD FETVEQYMKR AAIGSGYMEK PCLNPLNPNC PDTAPNKNST 310 320 330 340 350 360 QPPDVGAILS GGCYGYAAKH MHWPEELIVG GAKRNRSGHL RKAQALQSVV QLMTEKEMYD 370 380 390 400 410 420 QWQDNYKVHH LGWTQEKAAE VLNAWQRNFS REVEQLLRKQ SRIATNYDIY VFSSAALDDI 430 440 450 460 470 480 LAKFSHPSAL SIVIGVAVTV LYAFCTLLRW RDPVRGQSSV GVAGVLLMCF STAAGLGLSA 490 500 510 520 530 540 LLGIVFNAAS TQVVPFLALG LGVDHIFMLT AAYAESNRRE QTKLILKKVG PSILFSACST 550 560 570 580 590 600 AGSFFAAAFI PVPALKVFCL QAAIVMCSNL AAALLVFPAM ISLDLRRRTA GRADIFCCCF 610 620 630 640 650 660 PVWKEQPKVA PPVLPLNNNN GRGARHPKSC NNNRVPLPAQ NPLLEQRADI PGSSHSLASF 670 680 690 700 710 720 SLATFAFQHY TPFLMRSWVK FLTVMGFLAA LISSLYASTR LQDGLDIIDL VPKDSNEHKF 730 740 750 760 770 780 LDAQTRLFGF YSMYAVTQGN FEYPTQQQLL RDYHDSFVRV PHVIKNDNGG LPDFWLLLFS 790 800 810 820 830 840 EWLGNLQKIF DEEYRDGRLT KECWFPNASS DAILAYKLIV QTGHVDNPVD KELVLTNRLV 850 860 870 880 890 900 NSDGIINQRA FYNYLSAWAT NDVFAYGASQ GKLYPEPRQY FHQPNEYDLK IPKSLPLVYA 910 920 930 940 950 960 QMPFYLHGLT DTSQIKTLIG HIRDLSVKYE GFGLPNYPSG IPFIFWEQYM TLRSSLAMIL 970 980 990 1000 1010 1020 ACVLLAALVL VSLLLLSVWA AVLVILSVLA SLAQIFGAMT LLGIKLSAIP AVILILSVGM 1030 1040 1050 1060 1070 1080 MLCFNVLISL GFMTSVGNRQ RRVQLSMQMS LGPLVHGMLT SGVAVFMLST SPFEFVIRHF 1090 1100 1110 1120 1130 1140 CWLLLVVLCV GACNSLLVFP ILLSMVGPEA ELVPLEHPDR ISTPSPLPVR SSKRSGKSYV 1150 1160 1170 1180 1190 1200 VQGSRSSRGS CQKSHHHHHK DLNDPSLTTI TEEPQSWKSS NSSIQMPNDW TYQPREQRPA 1210 1220 1230 1240 1250 1260 SYAAPPPAYH KAAAQQHHQH QGPPTTPPPP FPTAYPPELQ SIVVQPEVTV ETTHSDSNTT 1270 1280 KVTATANIKV ELAMPGRAVR SYNFTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila patched gene encodes a putative membrane protein required for segmental patterning." Hooper J.E., Scott M.P. Cell 59:751-765(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A protein with several possible membrane-spanning domains encoded by the Drosophila segment polarity gene patched." Nakano Y., Guerrero I., Hidalgo A., Taylor A., Whittle J.R.S., Ingham P.W. Nature 341:508-513(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28999, M28418 Genomic DNA. Translation: AAA28696.1. X17558 mRNA. Translation: CAA35591.1. AE013599 Genomic DNA. Translation: AAF59062.1.
PIR	S06119.
RefSeq	NP_523661.2. NM_078937.3.
UniGene	Dm.2630.
3D structure databases
ProteinModelPortal	P18502.
SMR	P18502. Positions 390-427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-634N.
Protein family/group databases
TCDB	2.A.6.6.2. resistance-nodulation-cell division (RND) superfamily.
Proteomic databases
PaxDb	P18502.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089427; FBpp0088443; FBgn0003892.
GeneID	35851.
KEGG	dme:Dmel_CG2411.
UCSC	CG2411-RA. d. melanogaster.
Organism-specific databases
CTD	35851.
FlyBase	FBgn0003892. ptc.
Phylogenomic databases
eggNOG	NOG313603.
GeneTree	ENSGT00680000099777.
HOGENOM	HOG000228313.
InParanoid	P18502.
KO	K06225.
OMA	APDRDYL.
OrthoDB	EOG4BRV1N.
PhylomeDB	P18502.
Gene expression databases
Bgee	P18502.
GermOnline	CG2411. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003392. Patched. IPR000731. SSD. IPR004766. TM_rcpt_patched. [Graphical view]
Pfam	PF02460. Patched. 1 hit. [Graphical view]
TIGRFAMs	TIGR00918. 2A060602. 1 hit.
PROSITE	PS50156. SSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	35851.
NextBio	795513.

Entry information

Entry name

PTC_DROME

Accession

Primary (citable) accession number: P18502
Secondary accession number(s): Q9V4W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 2, 2001
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project