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P18494

- GLN3_YEAST

UniProt

P18494 - GLN3_YEAST

Protein

Nitrogen regulatory protein GLN3

Gene

GLN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri306 – 33025GATA-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    2. sequence-specific DNA binding transcription factor activity Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. nitrate assimilation Source: UniProtKB-KW
    2. nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Nitrate assimilation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30221-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrogen regulatory protein GLN3
    Gene namesi
    Name:GLN3
    Ordered Locus Names:YER040W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER040w.
    SGDiS000000842. GLN3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 730730Nitrogen regulatory protein GLN3PRO_0000083477Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei251 – 2511Phosphoserine1 Publication
    Modified residuei267 – 2671Phosphoserine1 Publication
    Modified residuei285 – 2851Phosphoserine1 Publication
    Modified residuei469 – 4691Phosphoserine2 Publications
    Modified residuei552 – 5521Phosphoserine2 Publications
    Modified residuei562 – 5621Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP18494.
    PaxDbiP18494.

    Expressioni

    Gene expression databases

    GenevestigatoriP18494.

    Interactioni

    Protein-protein interaction databases

    BioGridi36776. 149 interactions.
    DIPiDIP-2353N.
    IntActiP18494. 10 interactions.
    MINTiMINT-599718.
    STRINGi4932.YER040W.

    Structurei

    3D structure databases

    ProteinModelPortaliP18494.
    SMRiP18494. Positions 305-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi129 – 13799aaTAD

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi351 – 36111Arg/Lys-rich (basic)Add
    BLAST

    Domaini

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

    Sequence similaritiesi

    Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri306 – 33025GATA-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5641.
    GeneTreeiENSGT00750000117454.
    HOGENOMiHOG000112739.
    KOiK09184.
    OMAiVVPILPK.
    OrthoDBiEOG7TQVCN.

    Family and domain databases

    Gene3Di3.30.50.10. 1 hit.
    InterProiIPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00320. GATA. 1 hit.
    [Graphical view]
    PRINTSiPR00619. GATAZNFINGER.
    SMARTiSM00401. ZnF_GATA. 1 hit.
    [Graphical view]
    PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
    PS50114. GATA_ZN_FINGER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18494-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA    50
    FASGLNGGTF DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT 100
    NHMDDDIAMF DSLATTQPID IAASNQQNGE IAQLWDFNVD QFNMTPSNSS 150
    GSATISAPNS FTSDIPQYNH GSLGNSVSKS SLFPYNSSTS NSNINQPSIN 200
    NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN IQHPFLKKSD 250
    SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK 300
    KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI 350
    KKRISKKRAK QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS 400
    LSRVIPEEII RDNIGNTNNI LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN 450
    FNGASNANLN SNNLMRHNSN TVTPNFRRSS RRSSTSSNTS SSSKSSSRSV 500
    VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP RIISSANFNS 550
    NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD 600
    VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR 650
    PSSRKSHTSL LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG 700
    GKISEDNSTK GSSKESSAIA DELDWLKFGI 730
    Length:730
    Mass (Da):79,383
    Last modified:February 1, 1995 - v2
    Checksum:i3159E1844469942E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti474 – 4741P → G in AAA34645. (PubMed:1682800)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35267 Genomic DNA. Translation: AAA34645.1.
    U18796 Genomic DNA. Translation: AAB64575.1.
    BK006939 Genomic DNA. Translation: DAA07694.1.
    PIRiS50543.
    RefSeqiNP_010958.3. NM_001178931.3.

    Genome annotation databases

    EnsemblFungiiYER040W; YER040W; YER040W.
    GeneIDi856763.
    KEGGisce:YER040W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35267 Genomic DNA. Translation: AAA34645.1 .
    U18796 Genomic DNA. Translation: AAB64575.1 .
    BK006939 Genomic DNA. Translation: DAA07694.1 .
    PIRi S50543.
    RefSeqi NP_010958.3. NM_001178931.3.

    3D structure databases

    ProteinModelPortali P18494.
    SMRi P18494. Positions 305-372.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36776. 149 interactions.
    DIPi DIP-2353N.
    IntActi P18494. 10 interactions.
    MINTi MINT-599718.
    STRINGi 4932.YER040W.

    Proteomic databases

    MaxQBi P18494.
    PaxDbi P18494.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER040W ; YER040W ; YER040W .
    GeneIDi 856763.
    KEGGi sce:YER040W.

    Organism-specific databases

    CYGDi YER040w.
    SGDi S000000842. GLN3.

    Phylogenomic databases

    eggNOGi COG5641.
    GeneTreei ENSGT00750000117454.
    HOGENOMi HOG000112739.
    KOi K09184.
    OMAi VVPILPK.
    OrthoDBi EOG7TQVCN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30221-MONOMER.

    Miscellaneous databases

    NextBioi 982938.

    Gene expression databases

    Genevestigatori P18494.

    Family and domain databases

    Gene3Di 3.30.50.10. 1 hit.
    InterProi IPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00320. GATA. 1 hit.
    [Graphical view ]
    PRINTSi PR00619. GATAZNFINGER.
    SMARTi SM00401. ZnF_GATA. 1 hit.
    [Graphical view ]
    PROSITEi PS00344. GATA_ZN_FINGER_1. 1 hit.
    PS50114. GATA_ZN_FINGER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain."
      Minehart P.L., Magasanik B.
      Mol. Cell. Biol. 11:6216-6228(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Nine-amino-acid transactivation domain: establishment and prediction utilities."
      Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
      Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251; SER-469 AND SER-552, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267; SER-285; SER-469; SER-552 AND SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLN3_YEAST
    AccessioniPrimary (citable) accession number: P18494
    Secondary accession number(s): D3DLU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 589 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3