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P18494 (GLN3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrogen regulatory protein GLN3
Gene names
Name:GLN3
Ordered Locus Names:YER040W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length730 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.

Subcellular location

Nucleus.

Domain

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.4

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 GATA-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 730730Nitrogen regulatory protein GLN3
PRO_0000083477

Regions

Zinc finger306 – 33025GATA-type
Motif129 – 13799aaTAD
Compositional bias351 – 36111Arg/Lys-rich (basic)

Amino acid modifications

Modified residue2511Phosphoserine Ref.7
Modified residue2671Phosphoserine Ref.8
Modified residue2851Phosphoserine Ref.8
Modified residue4691Phosphoserine Ref.7 Ref.8
Modified residue5521Phosphoserine Ref.7 Ref.8
Modified residue5621Phosphoserine Ref.6 Ref.8

Experimental info

Sequence conflict4741P → G in AAA34645. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P18494 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 3159E1844469942E

FASTA73079,383
        10         20         30         40         50         60 
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA FASGLNGGTF 

        70         80         90        100        110        120 
DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT NHMDDDIAMF DSLATTQPID 

       130        140        150        160        170        180 
IAASNQQNGE IAQLWDFNVD QFNMTPSNSS GSATISAPNS FTSDIPQYNH GSLGNSVSKS 

       190        200        210        220        230        240 
SLFPYNSSTS NSNINQPSIN NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN 

       250        260        270        280        290        300 
IQHPFLKKSD SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK 

       310        320        330        340        350        360 
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI KKRISKKRAK 

       370        380        390        400        410        420 
QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS LSRVIPEEII RDNIGNTNNI 

       430        440        450        460        470        480 
LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN FNGASNANLN SNNLMRHNSN TVTPNFRRSS 

       490        500        510        520        530        540 
RRSSTSSNTS SSSKSSSRSV VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP 

       550        560        570        580        590        600 
RIISSANFNS NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD 

       610        620        630        640        650        660 
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR PSSRKSHTSL 

       670        680        690        700        710        720 
LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG GKISEDNSTK GSSKESSAIA 

       730 
DELDWLKFGI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain."
Minehart P.L., Magasanik B.
Mol. Cell. Biol. 11:6216-6228(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Nine-amino-acid transactivation domain: establishment and prediction utilities."
Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251; SER-469 AND SER-552, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267; SER-285; SER-469; SER-552 AND SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35267 Genomic DNA. Translation: AAA34645.1.
U18796 Genomic DNA. Translation: AAB64575.1.
BK006939 Genomic DNA. Translation: DAA07694.1.
PIRS50543.
RefSeqNP_010958.3. NM_001178931.3.

3D structure databases

ProteinModelPortalP18494.
SMRP18494. Positions 305-372.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36776. 149 interactions.
DIPDIP-2353N.
IntActP18494. 10 interactions.
MINTMINT-599718.
STRING4932.YER040W.

Proteomic databases

PaxDbP18494.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER040W; YER040W; YER040W.
GeneID856763.
KEGGsce:YER040W.

Organism-specific databases

CYGDYER040w.
SGDS000000842. GLN3.

Phylogenomic databases

eggNOGCOG5641.
GeneTreeENSGT00750000117454.
HOGENOMHOG000112739.
KOK09184.
OMAVVPILPK.
OrthoDBEOG7TQVCN.

Enzyme and pathway databases

BioCycYEAST:G3O-30221-MONOMER.

Gene expression databases

GenevestigatorP18494.

Family and domain databases

Gene3D3.30.50.10. 1 hit.
InterProIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00320. GATA. 1 hit.
[Graphical view]
PRINTSPR00619. GATAZNFINGER.
SMARTSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982938.

Entry information

Entry nameGLN3_YEAST
AccessionPrimary (citable) accession number: P18494
Secondary accession number(s): D3DLU0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families