Nitrogen regulatory protein GLN3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P18494 (GLN3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 119. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nitrogen regulatory protein GLN3

Gene names

Name:	GLN3
Ordered Locus Names:	YER040W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.
Subcellular location	Nucleus.
Domain	the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.4
Miscellaneous	Present with 589 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 GATA-type zinc finger.

Ontologies

Keywords
Biological process	Nitrate assimilation Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW nitrogen catabolite activation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 7568152. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 2153652. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay PubMed 12140287. Source: SGD nucleus Inferred from direct assay PubMed 10604478. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay PubMed 7608102. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype PubMed 8655543. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 730

730

Nitrogen regulatory protein GLN3

PRO_0000083477

Regions

Zinc finger

306 – 330

GATA-type

Motif

129 – 137

9aaTAD

Compositional bias

351 – 361

Arg/Lys-rich (basic)

Amino acid modifications

Modified residue

223

Phosphoserine Ref.7

Modified residue

224

Phosphoserine Ref.7

Modified residue

225

Phosphoserine Ref.7

Modified residue

249

Phosphoserine Ref.6

Modified residue

251

Phosphoserine Ref.7

Modified residue

257

Phosphoserine Ref.7

Modified residue

275

Phosphothreonine Ref.7

Modified residue

285

Phosphoserine Ref.7

Modified residue

469

Phosphoserine Ref.6 Ref.7

Modified residue

471

Phosphothreonine Ref.7

Modified residue

531

Phosphoserine Ref.7

Modified residue

552

Phosphoserine Ref.7

Modified residue

562

Phosphoserine Ref.7

Modified residue

689

Phosphoserine Ref.6 Ref.7

Experimental info

Sequence conflict

474

P → G in AAA34645. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P18494 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 3159E1844469942E
Show »

FASTA

730

79,383

        10         20         30         40         50         60 
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA FASGLNGGTF 

        70         80         90        100        110        120 
DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT NHMDDDIAMF DSLATTQPID 

       130        140        150        160        170        180 
IAASNQQNGE IAQLWDFNVD QFNMTPSNSS GSATISAPNS FTSDIPQYNH GSLGNSVSKS 

       190        200        210        220        230        240 
SLFPYNSSTS NSNINQPSIN NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN 

       250        260        270        280        290        300 
IQHPFLKKSD SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK 

       310        320        330        340        350        360 
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI KKRISKKRAK 

       370        380        390        400        410        420 
QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS LSRVIPEEII RDNIGNTNNI 

       430        440        450        460        470        480 
LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN FNGASNANLN SNNLMRHNSN TVTPNFRRSS 

       490        500        510        520        530        540 
RRSSTSSNTS SSSKSSSRSV VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP 

       550        560        570        580        590        600 
RIISSANFNS NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD 

       610        620        630        640        650        660 
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR PSSRKSHTSL 

       670        680        690        700        710        720 
LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG GKISEDNSTK GSSKESSAIA 

       730 
DELDWLKFGI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain." Minehart P.L., Magasanik B. Mol. Cell. Biol. 11:6216-6228(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Nine-amino-acid transactivation domain: establishment and prediction utilities." Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M. Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249; SER-469 AND SER-689, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223; SER-224; SER-225; SER-251; SER-257; THR-275; SER-285; SER-469; THR-471; SER-531; SER-552; SER-562 AND SER-689, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35267 Genomic DNA. Translation: AAA34645.1. U18796 Genomic DNA. Translation: AAB64575.1. BK006939 Genomic DNA. Translation: DAA07694.1.
PIR	S50543.
RefSeq	NP_010958.3. NM_001178931.3.
3D structure databases
ProteinModelPortal	P18494.
SMR	P18494. Positions 306-353.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2353N.
IntAct	P18494. 9 interactions.
MINT	MINT-599718.
STRING	4932.YER040W.
Proteomic databases
PaxDb	P18494.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER040W; YER040W; YER040W.
GeneID	856763.
KEGG	sce:YER040W. sce:YER043C.
Organism-specific databases
CYGD	YER040w.
SGD	S000000842. GLN3.
Phylogenomic databases
eggNOG	COG5641.
GeneTree	ENSGT00550000074470.
HOGENOM	HOG000112739.
KO	K01251. K09184.
OMA	VVPILPK.
OrthoDB	EOG4W11PS.
Gene expression databases
Genevestigator	P18494.
GermOnline	YER040W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.50.10. 1 hit.
InterPro	IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00320. GATA. 1 hit. [Graphical view]
PRINTS	PR00619. GATAZNFINGER.
SMART	SM00401. ZnF_GATA. 1 hit. [Graphical view]
PROSITE	PS00344. GATA_ZN_FINGER_1. 1 hit. PS50114. GATA_ZN_FINGER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982938.

Entry information

Entry name

GLN3_YEAST

Accession

Primary (citable) accession number: P18494
Secondary accession number(s): D3DLU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents