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P18494

- GLN3_YEAST

UniProt

P18494 - GLN3_YEAST

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Protein

Nitrogen regulatory protein GLN3

Gene

GLN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri306 – 33025GATA-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. sequence-specific DNA binding transcription factor activity Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
  2. nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Nitrate assimilation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30221-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein GLN3
Gene namesi
Name:GLN3
Ordered Locus Names:YER040W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER040w.
SGDiS000000842. GLN3.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Nitrogen regulatory protein GLN3PRO_0000083477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei251 – 2511Phosphoserine1 Publication
Modified residuei267 – 2671Phosphoserine1 Publication
Modified residuei285 – 2851Phosphoserine1 Publication
Modified residuei469 – 4691Phosphoserine2 Publications
Modified residuei552 – 5521Phosphoserine2 Publications
Modified residuei562 – 5621Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18494.
PaxDbiP18494.

Expressioni

Gene expression databases

GenevestigatoriP18494.

Interactioni

Protein-protein interaction databases

BioGridi36776. 149 interactions.
DIPiDIP-2353N.
IntActiP18494. 10 interactions.
MINTiMINT-599718.
STRINGi4932.YER040W.

Structurei

3D structure databases

ProteinModelPortaliP18494.
SMRiP18494. Positions 305-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi129 – 13799aaTAD

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi351 – 36111Arg/Lys-rich (basic)Add
BLAST

Domaini

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri306 – 33025GATA-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5641.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000112739.
InParanoidiP18494.
KOiK09184.
OMAiVVPILPK.
OrthoDBiEOG7TQVCN.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18494-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA
60 70 80 90 100
FASGLNGGTF DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT
110 120 130 140 150
NHMDDDIAMF DSLATTQPID IAASNQQNGE IAQLWDFNVD QFNMTPSNSS
160 170 180 190 200
GSATISAPNS FTSDIPQYNH GSLGNSVSKS SLFPYNSSTS NSNINQPSIN
210 220 230 240 250
NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN IQHPFLKKSD
260 270 280 290 300
SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK
310 320 330 340 350
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI
360 370 380 390 400
KKRISKKRAK QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS
410 420 430 440 450
LSRVIPEEII RDNIGNTNNI LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN
460 470 480 490 500
FNGASNANLN SNNLMRHNSN TVTPNFRRSS RRSSTSSNTS SSSKSSSRSV
510 520 530 540 550
VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP RIISSANFNS
560 570 580 590 600
NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD
610 620 630 640 650
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR
660 670 680 690 700
PSSRKSHTSL LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG
710 720 730
GKISEDNSTK GSSKESSAIA DELDWLKFGI
Length:730
Mass (Da):79,383
Last modified:February 1, 1995 - v2
Checksum:i3159E1844469942E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti474 – 4741P → G in AAA34645. (PubMed:1682800)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35267 Genomic DNA. Translation: AAA34645.1.
U18796 Genomic DNA. Translation: AAB64575.1.
BK006939 Genomic DNA. Translation: DAA07694.1.
PIRiS50543.
RefSeqiNP_010958.3. NM_001178931.3.

Genome annotation databases

EnsemblFungiiYER040W; YER040W; YER040W.
GeneIDi856763.
KEGGisce:YER040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35267 Genomic DNA. Translation: AAA34645.1 .
U18796 Genomic DNA. Translation: AAB64575.1 .
BK006939 Genomic DNA. Translation: DAA07694.1 .
PIRi S50543.
RefSeqi NP_010958.3. NM_001178931.3.

3D structure databases

ProteinModelPortali P18494.
SMRi P18494. Positions 305-372.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36776. 149 interactions.
DIPi DIP-2353N.
IntActi P18494. 10 interactions.
MINTi MINT-599718.
STRINGi 4932.YER040W.

Proteomic databases

MaxQBi P18494.
PaxDbi P18494.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER040W ; YER040W ; YER040W .
GeneIDi 856763.
KEGGi sce:YER040W.

Organism-specific databases

CYGDi YER040w.
SGDi S000000842. GLN3.

Phylogenomic databases

eggNOGi COG5641.
GeneTreei ENSGT00760000119221.
HOGENOMi HOG000112739.
InParanoidi P18494.
KOi K09184.
OMAi VVPILPK.
OrthoDBi EOG7TQVCN.

Enzyme and pathway databases

BioCyci YEAST:G3O-30221-MONOMER.

Miscellaneous databases

NextBioi 982938.

Gene expression databases

Genevestigatori P18494.

Family and domain databases

Gene3Di 3.30.50.10. 1 hit.
InterProi IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00320. GATA. 1 hit.
[Graphical view ]
PRINTSi PR00619. GATAZNFINGER.
SMARTi SM00401. ZnF_GATA. 1 hit.
[Graphical view ]
PROSITEi PS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain."
    Minehart P.L., Magasanik B.
    Mol. Cell. Biol. 11:6216-6228(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Nine-amino-acid transactivation domain: establishment and prediction utilities."
    Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
    Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251; SER-469 AND SER-552, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267; SER-285; SER-469; SER-552 AND SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLN3_YEAST
AccessioniPrimary (citable) accession number: P18494
Secondary accession number(s): D3DLU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3