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Protein

Nitrogen regulatory protein GLN3

Gene

GLN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri306 – 330GATA-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • nitrate assimilation Source: UniProtKB-KW
  • nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Nitrate assimilation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30221-MONOMER.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein GLN3
Gene namesi
Name:GLN3
Ordered Locus Names:YER040W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER040W.
SGDiS000000842. GLN3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834771 – 730Nitrogen regulatory protein GLN3Add BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei285PhosphoserineCombined sources1
Modified residuei469PhosphoserineCombined sources1
Modified residuei552PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18494.
PRIDEiP18494.

PTM databases

iPTMnetiP18494.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36776. 152 interactors.
DIPiDIP-2353N.
IntActiP18494. 11 interactors.
MINTiMINT-599718.

Structurei

3D structure databases

ProteinModelPortaliP18494.
SMRiP18494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi129 – 1379aaTAD9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi351 – 361Arg/Lys-rich (basic)Add BLAST11

Domaini

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri306 – 330GATA-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000119221.
HOGENOMiHOG000112739.
InParanoidiP18494.
KOiK09184.
OMAiDELDWLK.
OrthoDBiEOG092C43RR.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA
60 70 80 90 100
FASGLNGGTF DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT
110 120 130 140 150
NHMDDDIAMF DSLATTQPID IAASNQQNGE IAQLWDFNVD QFNMTPSNSS
160 170 180 190 200
GSATISAPNS FTSDIPQYNH GSLGNSVSKS SLFPYNSSTS NSNINQPSIN
210 220 230 240 250
NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN IQHPFLKKSD
260 270 280 290 300
SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK
310 320 330 340 350
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI
360 370 380 390 400
KKRISKKRAK QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS
410 420 430 440 450
LSRVIPEEII RDNIGNTNNI LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN
460 470 480 490 500
FNGASNANLN SNNLMRHNSN TVTPNFRRSS RRSSTSSNTS SSSKSSSRSV
510 520 530 540 550
VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP RIISSANFNS
560 570 580 590 600
NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD
610 620 630 640 650
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR
660 670 680 690 700
PSSRKSHTSL LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG
710 720 730
GKISEDNSTK GSSKESSAIA DELDWLKFGI
Length:730
Mass (Da):79,383
Last modified:February 1, 1995 - v2
Checksum:i3159E1844469942E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti474P → G in AAA34645 (PubMed:1682800).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35267 Genomic DNA. Translation: AAA34645.1.
U18796 Genomic DNA. Translation: AAB64575.1.
BK006939 Genomic DNA. Translation: DAA07694.1.
PIRiS50543.
RefSeqiNP_010958.3. NM_001178931.3.

Genome annotation databases

EnsemblFungiiYER040W; YER040W; YER040W.
GeneIDi856763.
KEGGisce:YER040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35267 Genomic DNA. Translation: AAA34645.1.
U18796 Genomic DNA. Translation: AAB64575.1.
BK006939 Genomic DNA. Translation: DAA07694.1.
PIRiS50543.
RefSeqiNP_010958.3. NM_001178931.3.

3D structure databases

ProteinModelPortaliP18494.
SMRiP18494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36776. 152 interactors.
DIPiDIP-2353N.
IntActiP18494. 11 interactors.
MINTiMINT-599718.

PTM databases

iPTMnetiP18494.

Proteomic databases

MaxQBiP18494.
PRIDEiP18494.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER040W; YER040W; YER040W.
GeneIDi856763.
KEGGisce:YER040W.

Organism-specific databases

EuPathDBiFungiDB:YER040W.
SGDiS000000842. GLN3.

Phylogenomic databases

GeneTreeiENSGT00760000119221.
HOGENOMiHOG000112739.
InParanoidiP18494.
KOiK09184.
OMAiDELDWLK.
OrthoDBiEOG092C43RR.

Enzyme and pathway databases

BioCyciYEAST:G3O-30221-MONOMER.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.

Miscellaneous databases

PROiP18494.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLN3_YEAST
AccessioniPrimary (citable) accession number: P18494
Secondary accession number(s): D3DLU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.