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Protein

Synaptobrevin

Gene

Syb

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Unknown, but synaptobrevins are presumed to be involved in targeting and fusion of synaptic vesicles with the presynaptic membrane as well as in neurotransmitter release.

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: GO_Central

GO - Biological processi

  • exocytosis Source: GO_Central
  • lumen formation, open tracheal system Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • synaptic vesicle docking Source: FlyBase
  • synaptic vesicle transport Source: FlyBase
  • vesicle fusion Source: GO_Central
  • vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-DME-210500. Glutamate Neurotransmitter Release Cycle.
R-DME-264642. Acetylcholine Neurotransmitter Release Cycle.
R-DME-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptobrevin
Gene namesi
Name:Syb
ORF Names:CG12210
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003660. Syb.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 110110CytoplasmicSequence analysisAdd
BLAST
Transmembranei111 – 13020Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini131 – 15222VesicularSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB-KW
  • neuron projection Source: UniProtKB-SubCell
  • plasma membrane Source: FlyBase
  • synaptic vesicle Source: FlyBase
  • synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152SynaptobrevinPRO_0000206741Add
BLAST

Proteomic databases

PaxDbiP18489.
PRIDEiP18489.

Expressioni

Tissue specificityi

Not nervous system specific; abundant in cells of the gut and Malpighian tubules.1 Publication

Gene expression databases

BgeeiFBgn0003660.
ExpressionAtlasiP18489. differential.
GenevisibleiP18489. DM.

Interactioni

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi61901. 60 interactions.
IntActiP18489. 2 interactions.
STRINGi7227.FBpp0271782.

Structurei

3D structure databases

ProteinModelPortaliP18489.
SMRiP18489. Positions 46-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 10761v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 3131Asn-richAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
InParanoidiP18489.
OMAiNANDNHY.
OrthoDBiEOG091G0MPE.
PhylomeDBiP18489.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: The ratio of isoform Syb-A to isoform Syb-B is highly regulated during development.
Isoform Syb-A (identifier: P18489-1) [UniParc]FASTAAdd to basket
Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENNEAPSPS GSNNNDFPIL PPPPNANDNY NQFGDHQIRN NNAAQKKLQQ
60 70 80 90 100
TQAKVDEVVG IMRVNVEKVL ERDQKLSELG ERADQLEQGA SQFEQQAGKL
110 120 130 140 150
KRKQWWANMK MMIILGVIAV VLLIIVLVSV WPSSSDSGSG GGNKAITQAP

PH
Length:152
Mass (Da):16,712
Last modified:November 8, 2005 - v3
Checksum:iFB5E7DE5789DAA09
GO
Isoform Syb-B (identifier: P18489-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     130-132: VWP → LFN
     133-152: Missing.

Show »
Length:132
Mass (Da):14,867
Checksum:i46AD06996D28D4D2
GO
Isoform A (identifier: P18489-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-39: DFPILPPPPNANDNYNQFGDHQIR → E

Note: No experimental confirmation available.
Show »
Length:129
Mass (Da):14,079
Checksum:i7F6F037279763CE3
GO
Isoform D (identifier: P18489-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-39: DFPILPPPPNANDNYNQFGDHQIR → E
     130-132: VWP → LFN
     133-152: Missing.

Note: No experimental confirmation available.
Show »
Length:109
Mass (Da):12,234
Checksum:i175CD3BACCC32DF3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931F → S in AAA28924 (PubMed:8406010).Curated
Sequence conflicti93 – 931F → S in AAA28923 (PubMed:8406010).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei16 – 3924DFPIL…DHQIR → E in isoform A and isoform D. 1 PublicationVSP_016074Add
BLAST
Alternative sequencei130 – 1323VWP → LFN in isoform D and isoform Syb-B. 1 PublicationVSP_006327
Alternative sequencei133 – 15220Missing in isoform D and isoform Syb-B. 1 PublicationVSP_006328Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76200 mRNA. Translation: CAA53793.1.
L14270 Genomic DNA. Translation: AAA28923.1.
L14270 Genomic DNA. Translation: AAA28924.1.
AE013599 Genomic DNA. Translation: AAF58820.1.
AE013599 Genomic DNA. Translation: AAM68777.1.
AE013599 Genomic DNA. Translation: ACL83087.1.
AE013599 Genomic DNA. Translation: ACL83088.1.
BT003769 mRNA. Translation: AAO41448.1.
BT009942 mRNA. Translation: AAQ22411.1.
PIRiJC1521.
JC1522.
RefSeqiNP_001137633.1. NM_001144161.2. [P18489-1]
NP_001137634.1. NM_001144162.2. [P18489-4]
NP_001286278.1. NM_001299349.1. [P18489-3]
NP_523680.1. NM_078956.4. [P18489-3]
NP_724906.1. NM_165756.3. [P18489-2]
UniGeneiDm.19412.

Genome annotation databases

EnsemblMetazoaiFBtr0273274; FBpp0271782; FBgn0003660. [P18489-1]
GeneIDi36080.
KEGGidme:Dmel_CG12210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76200 mRNA. Translation: CAA53793.1.
L14270 Genomic DNA. Translation: AAA28923.1.
L14270 Genomic DNA. Translation: AAA28924.1.
AE013599 Genomic DNA. Translation: AAF58820.1.
AE013599 Genomic DNA. Translation: AAM68777.1.
AE013599 Genomic DNA. Translation: ACL83087.1.
AE013599 Genomic DNA. Translation: ACL83088.1.
BT003769 mRNA. Translation: AAO41448.1.
BT009942 mRNA. Translation: AAQ22411.1.
PIRiJC1521.
JC1522.
RefSeqiNP_001137633.1. NM_001144161.2. [P18489-1]
NP_001137634.1. NM_001144162.2. [P18489-4]
NP_001286278.1. NM_001299349.1. [P18489-3]
NP_523680.1. NM_078956.4. [P18489-3]
NP_724906.1. NM_165756.3. [P18489-2]
UniGeneiDm.19412.

3D structure databases

ProteinModelPortaliP18489.
SMRiP18489. Positions 46-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61901. 60 interactions.
IntActiP18489. 2 interactions.
STRINGi7227.FBpp0271782.

Proteomic databases

PaxDbiP18489.
PRIDEiP18489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0273274; FBpp0271782; FBgn0003660. [P18489-1]
GeneIDi36080.
KEGGidme:Dmel_CG12210.

Organism-specific databases

CTDi36080.
FlyBaseiFBgn0003660. Syb.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
InParanoidiP18489.
OMAiNANDNHY.
OrthoDBiEOG091G0MPE.
PhylomeDBiP18489.

Enzyme and pathway databases

ReactomeiR-DME-210500. Glutamate Neurotransmitter Release Cycle.
R-DME-264642. Acetylcholine Neurotransmitter Release Cycle.
R-DME-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

GenomeRNAii36080.
PROiP18489.

Gene expression databases

BgeeiFBgn0003660.
ExpressionAtlasiP18489. differential.
GenevisibleiP18489. DM.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYB_DROME
AccessioniPrimary (citable) accession number: P18489
Secondary accession number(s): B7YZD5
, B7YZD6, Q3HKB4, Q8MKX1, Q9V5I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.