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Protein

Synaptobrevin

Gene

Syb

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Unknown, but synaptobrevins are presumed to be involved in targeting and fusion of synaptic vesicles with the presynaptic membrane as well as in neurotransmitter release.

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: GO_Central

GO - Biological processi

  • exocytosis Source: GO_Central
  • lumen formation, open tracheal system Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • synaptic vesicle docking Source: FlyBase
  • vesicle fusion Source: GO_Central
  • vesicle-mediated transport Source: FlyBase

Enzyme and pathway databases

ReactomeiR-DME-210500. Glutamate Neurotransmitter Release Cycle.
R-DME-264642. Acetylcholine Neurotransmitter Release Cycle.
R-DME-6811440. Retrograde transport at the Trans-Golgi-Network.
R-DME-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-DME-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptobrevin
Gene namesi
Name:Syb
ORF Names:CG12210
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003660. Syb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 110CytoplasmicSequence analysisAdd BLAST110
Transmembranei111 – 130Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST20
Topological domaini131 – 152VesicularSequence analysisAdd BLAST22

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067411 – 152SynaptobrevinAdd BLAST152

Proteomic databases

PaxDbiP18489.
PRIDEiP18489.

Expressioni

Tissue specificityi

Not nervous system specific; abundant in cells of the gut and Malpighian tubules.1 Publication

Gene expression databases

BgeeiFBgn0003660.
ExpressionAtlasiP18489. differential.
GenevisibleiP18489. DM.

Interactioni

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi61901. 62 interactors.
IntActiP18489. 2 interactors.
STRINGi7227.FBpp0271782.

Structurei

3D structure databases

ProteinModelPortaliP18489.
SMRiP18489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 107v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 31Asn-richAdd BLAST31

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
InParanoidiP18489.
KOiK13504.
OMAiVPYDPYI.
OrthoDBiEOG091G0MPE.
PhylomeDBiP18489.

Family and domain databases

InterProiView protein in InterPro
IPR001388. Synaptobrevin.
PfamiView protein in Pfam
PF00957. Synaptobrevin. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiView protein in PROSITE
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: The ratio of isoform Syb-A to isoform Syb-B is highly regulated during development.
Isoform Syb-A (identifier: P18489-1) [UniParc]FASTAAdd to basket
Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENNEAPSPS GSNNNDFPIL PPPPNANDNY NQFGDHQIRN NNAAQKKLQQ
60 70 80 90 100
TQAKVDEVVG IMRVNVEKVL ERDQKLSELG ERADQLEQGA SQFEQQAGKL
110 120 130 140 150
KRKQWWANMK MMIILGVIAV VLLIIVLVSV WPSSSDSGSG GGNKAITQAP

PH
Length:152
Mass (Da):16,712
Last modified:November 8, 2005 - v3
Checksum:iFB5E7DE5789DAA09
GO
Isoform Syb-B (identifier: P18489-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     130-132: VWP → LFN
     133-152: Missing.

Show »
Length:132
Mass (Da):14,867
Checksum:i46AD06996D28D4D2
GO
Isoform A (identifier: P18489-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-39: DFPILPPPPNANDNYNQFGDHQIR → E

Note: No experimental confirmation available.
Show »
Length:129
Mass (Da):14,079
Checksum:i7F6F037279763CE3
GO
Isoform D (identifier: P18489-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-39: DFPILPPPPNANDNYNQFGDHQIR → E
     130-132: VWP → LFN
     133-152: Missing.

Note: No experimental confirmation available.
Show »
Length:109
Mass (Da):12,234
Checksum:i175CD3BACCC32DF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93F → S in AAA28924 (PubMed:8406010).Curated1
Sequence conflicti93F → S in AAA28923 (PubMed:8406010).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01607416 – 39DFPIL…DHQIR → E in isoform A and isoform D. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_006327130 – 132VWP → LFN in isoform D and isoform Syb-B. 1 Publication3
Alternative sequenceiVSP_006328133 – 152Missing in isoform D and isoform Syb-B. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76200 mRNA. Translation: CAA53793.1.
L14270 Genomic DNA. Translation: AAA28923.1.
L14270 Genomic DNA. Translation: AAA28924.1.
AE013599 Genomic DNA. Translation: AAF58820.1.
AE013599 Genomic DNA. Translation: AAM68777.1.
AE013599 Genomic DNA. Translation: ACL83087.1.
AE013599 Genomic DNA. Translation: ACL83088.1.
BT003769 mRNA. Translation: AAO41448.1.
BT009942 mRNA. Translation: AAQ22411.1.
PIRiJC1521.
JC1522.
RefSeqiNP_001137633.1. NM_001144161.2. [P18489-1]
NP_001137634.1. NM_001144162.2. [P18489-4]
NP_001286278.1. NM_001299349.1. [P18489-3]
NP_523680.1. NM_078956.4. [P18489-3]
NP_724906.1. NM_165756.3. [P18489-2]
UniGeneiDm.19412.

Genome annotation databases

EnsemblMetazoaiFBtr0088362; FBpp0087450; FBgn0003660. [P18489-3]
FBtr0088363; FBpp0087451; FBgn0003660. [P18489-2]
FBtr0273274; FBpp0271782; FBgn0003660. [P18489-1]
FBtr0273275; FBpp0271783; FBgn0003660. [P18489-4]
FBtr0339455; FBpp0308541; FBgn0003660. [P18489-3]
GeneIDi36080.
KEGGidme:Dmel_CG12210.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYB_DROME
AccessioniPrimary (citable) accession number: P18489
Secondary accession number(s): B7YZD5
, B7YZD6, Q3HKB4, Q8MKX1, Q9V5I6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 8, 2005
Last modified: October 25, 2017
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families